RchiOBHm_Chr1g0384661

Belongs to the universal ribosomal protein uS5 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0001359
Physical Location & Seq
Forward (+)
68422463 .. 68427823
5361 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1479 bp
ATGTCCAAATCGCAAGCGTTCTCGTGGTCGCGTCTCATCTTCAATCAAGCAATCAACAAAACCCGCAACGCCCCACTCCCCTTCATCTCCTCCACCTTACCACACTTGCTCAGGTCCTCTGGGCAGACCCATTACTATTCCACAGTATCAGACAGAGTAGTCCGAGACCTTCTGGCCGAGGTCGAGAGAGAAAAGCAGAGAGAAAGGGAACAGAGGAAAAGGGCTGGTCTCGACACCAAAGACATTGACCAAGAAGATGAGGAGGACTACATGCGTGTCACTCCATTGATAGACAAGCTTGAGAAGGAGAAGCTCAAGGCCACTGGGGATCTCAATTCCTATGAGGAGCCCACCGATTCGGATAGTGACGACGACGACGAGCGCTTTACTCCCGATGCCGTCAAGAAGAGGTTTGATGAGTTTCAGAGGAAGTTCACGCGACACGAGGAATTGCTCAAGAATTTCACACAGGCTGAGACACTTGATGATGCTTTTAAATGGATGAATAAAATTGACAAGTTTGAGCAAAAGCATTTCCGGCTTCGCCCTGAATATAGAGTCATTGGTGAATTGATGAACCGTCTAAAAGTAGCTGAGGGCAAGGATAAATTCATTCTGCAGCACAAACTAAACAGGGCTATGCGATTGCTGCAGTGGAAGGAAGCCTATGATCCAAACGATCCTGCCAACTATGGAGTCATTCAGGAACAACTGGAGCCCTCATTTGATGTTCTAGAAGATGCTGGATTTGAAAAAGAAAAAAAAATTATTCGAGGAGTTGATGATGATGATGATGAAGAATTCAATGACATGAAGGAGAAAGATGACATACTATTAGAGAAGCTAAATGCTATTGACAAGAAACTTGAAGAGAAGCTAGCAGAGTTGGATCATACTTTTGGAAAGAAGGGAAAGGTTCTAGAGGAAGAGATCAGAGATCTAGCAGAGGAAAGAAATGATTTGACGGAGAAGAAGAGAAGACCTCTCTATAGGAAAGGTTTCGATGTTAGATTAATTGATGTGAACCGGACTTGTAAAGTTACAAAGGGAGGACAAGTGGTCAAATACACTGCTATATTAGTTTGTGGAAACTATCATGGTGTTGCAGGTTATGCTAAAGCAAAAGGTCCAGCTGTCCCCCTCGCCCTCCAGAAGGCATATGAGAAATGCTTTCAGAATCTCCACTATGTAGAACGACATGAGGAGCATACAATTGCGCATGCAATACAAACTACATATAAAAAAACAAAGGTTTATCTCTGGCCTGCACCCACCAGGACGGGTATGAAAGCAGGAAGAACTGTCGAAACCATTCTAAACTTGGCAGGTTTCAATAATGTCAAGTCTAAGGTTGTTGGTTCAAGGAATCCTCATAATACAGTGAAGGCTGTTTTCAAAGCCCTTAATGCGATTGAAACGCCCAAGGATGTTCAAGAAAAGTTTGGACGCACTGTTGTTGAGAAGTCCCTCGTATCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S5 Ribosomal_S5_C

Protein Analysis

492

Amino Acids

57.15

Weight (kDa)

7.67

Isoelectric Point (pI)

37.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S5 PF00333 334 - 398 7e-18 Ribosomal protein S5, N-terminal domain
Ribosomal_S5_C PF03719 412 - 480 2.7e-23 Ribosomal protein S5, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 158
Acc16I TGCGCA 1 cut(s) 1218
Acc36I ACCTGC 2 cut(s) 1097, 1316
AccII CGCG 2 cut(s) 31, 439
AciI CCGC 1 cut(s) 64
AclWI GGATC 4 cut(s) 336, 665, 674, 897
AcoI YGGCCR 1 cut(s) 174
AcsI RAATTY 3 cut(s) 460, 608, 800
AfeI AGCGCT 1 cut(s) 383
AfiI CCNNNNNNNGG 1 cut(s) 1153
AgsI TTSAA 9 cut(s) 43, 752, 805, 869, 1333, 1362, 1396, 1415, 1433
AhdI GACNNNNNGTC 1 cut(s) 1058
AjnI CCWGG 1 cut(s) 1274
AluBI AGCT 6 cut(s) 298, 313, 593, 844, 877, 1133
AluI AGCT 6 cut(s) 298, 313, 593, 844, 877, 1133
Alw26I GTCTC 4 cut(s) 38, 159, 233, 470
AlwI GGATC 4 cut(s) 336, 665, 674, 897
Aor51HI AGCGCT 1 cut(s) 383
AoxI GGCC 3 cut(s) 174, 318, 1262
ApeKI GCWGC 2 cut(s) 619, 649
ApoI RAATTY 3 cut(s) 460, 608, 800
AseI ATTAAT 1 cut(s) 1013
Asp700I GAANNNNTTC 2 cut(s) 998, 1311
AspLEI GCGC 2 cut(s) 384, 1219
AspS9I GGNCC 2 cut(s) 114, 1127
AsuHPI GGTGA 1 cut(s) 578
AsuNHI GCTAGC 1 cut(s) 877
AvaII GGWCC 2 cut(s) 114, 1127
BanII GRGCYC 2 cut(s) 351, 720
BauI CACGAG 2 cut(s) 22, 443
BbsI GAAGAC 1 cut(s) 985
BbvCI CCTCAGC 1 cut(s) 594
BbvI GCAGC 2 cut(s) 631, 636
BceAI ACGGC 1 cut(s) 383
BciT130I CCWGG 1 cut(s) 1276
BcoDI GTCTC 4 cut(s) 38, 159, 233, 470
BfaI CTAG 4 cut(s) 734, 878, 920, 941
BfmI CTRYAG 3 cut(s) 617, 650, 988
BfoI RGCGCY 1 cut(s) 385
BfuAI ACCTGC 2 cut(s) 1097, 1316
BglII AGATCT 1 cut(s) 937
BisI GCNGC 2 cut(s) 620, 650
BlsI GCNGC 2 cut(s) 621, 651
Bme1390I CCNGG 1 cut(s) 1276
Bme18I GGWCC 2 cut(s) 114, 1127
BmeRI GACNNNNNGTC 1 cut(s) 1058
BmgT120I GGNCC 2 cut(s) 114, 1127
BmiI GGNNCC 2 cut(s) 348, 717
BmrFI CCNGG 1 cut(s) 1276
BmrI ACTGGG 1 cut(s) 333
BmsI GCATC 3 cut(s) 385, 478, 730
BmtI GCTAGC 1 cut(s) 881
BmuI ACTGGG 1 cut(s) 333
BpiI GAAGAC 1 cut(s) 985
BplI GAGNNNNNCTC 2 cut(s) 967, 999
BpmI CTGGAG 2 cut(s) 734, 1133
Bpu10I CCTNAGC 2 cut(s) 110, 594
BpuEI CTTGAG 3 cut(s) 299, 320, 440
BsaI GGTCTC 2 cut(s) 159, 233
BsaJI CCNNGG 2 cut(s) 177, 1422
BsaWI WCCGGW 1 cut(s) 1026
BsaXI ACNNNNNCTCC 2 cut(s) 1041, 1071
Bsc4I CCNNNNNNNGG 1 cut(s) 1153
Bse1I ACTGG 2 cut(s) 328, 717
BseBI CCWGG 1 cut(s) 1276
BseDI CCNNGG 2 cut(s) 177, 1422
BseGI GGATG 2 cut(s) 507, 1432
BseLI CCNNNNNNNGG 1 cut(s) 1153
BseMII CTCAG 3 cut(s) 124, 465, 585
BseNI ACTGG 2 cut(s) 328, 717
BseRI GAGGAG 5 cut(s) 79, 275, 359, 789, 1217
BseXI GCAGC 2 cut(s) 631, 636
BsgI GTGCAG 1 cut(s) 1251
Bsh1236I CGCG 2 cut(s) 31, 439
BshFI GGCC 3 cut(s) 176, 320, 1264
BsiSI CCGG 2 cut(s) 538, 1027
BslFI GGGAC 2 cut(s) 1121, 1450
BslI CCNNNNNNNGG 1 cut(s) 1153
BsmAI GTCTC 4 cut(s) 38, 159, 233, 470
BsmBI CGTCTC 1 cut(s) 38
BsmFI GGGAC 2 cut(s) 1121, 1450
BsnI GGCC 3 cut(s) 176, 320, 1264
Bso31I GGTCTC 2 cut(s) 159, 233
Bsp1286I GDGCHC 2 cut(s) 351, 720
Bsp143I GATC 6 cut(s) 328, 670, 679, 889, 930, 937
BspACI CCGC 1 cut(s) 64
BspANI GGCC 3 cut(s) 176, 320, 1264
BspCNI CTCAG 3 cut(s) 123, 466, 586
BspFNI CGCG 2 cut(s) 31, 439
BspLI GGNNCC 2 cut(s) 348, 717
BspMAI CTGCAG 2 cut(s) 621, 654
BspMI ACCTGC 2 cut(s) 1097, 1316
BspOI GCTAGC 1 cut(s) 881
BspPI GGATC 4 cut(s) 336, 665, 674, 897
BspTNI GGTCTC 2 cut(s) 159, 233
BsrI ACTGG 2 cut(s) 328, 717
BssECI CCNNGG 2 cut(s) 177, 1422
BssMI GATC 6 cut(s) 328, 670, 679, 889, 930, 937
BssSI CACGAG 2 cut(s) 22, 443
BssT1I CCWWGG 1 cut(s) 1422
Bst2BI CACGAG 2 cut(s) 22, 443
Bst2UI CCWGG 1 cut(s) 1276
Bst4CI ACNGT 5 cut(s) 145, 581, 1303, 1381, 1453
Bst6I CTCTTC 4 cut(s) 401, 864, 921, 968
BstAPI GCANNNNNTGC 1 cut(s) 1112
BstC8I GCNNGC 4 cut(s) 15, 879, 1221, 1266
BstDEI CTNAG 4 cut(s) 110, 474, 594, 1347
BstENI CCTNNNNNAGG 1 cut(s) 1151
BstF5I GGATG 2 cut(s) 507, 1432
BstFNI CGCG 2 cut(s) 31, 439
BstH2I RGCGCY 1 cut(s) 385
BstHHI GCGC 2 cut(s) 384, 1219
BstKTI GATC 6 cut(s) 331, 673, 682, 892, 933, 940
BstMAI GTCTC 4 cut(s) 38, 159, 233, 470
BstMBI GATC 6 cut(s) 328, 670, 679, 889, 930, 937
BstMWI GCNNNNNNNGC 4 cut(s) 538, 649, 1112, 1406
BstNI CCWGG 1 cut(s) 1276
BstNSI RCATGY 2 cut(s) 274, 1223
BstSCI CCNGG 1 cut(s) 1274
BstSFI CTRYAG 3 cut(s) 617, 650, 988
BstUI CGCG 2 cut(s) 31, 439
BstV1I GCAGC 2 cut(s) 631, 636
BstV2I GAAGAC 1 cut(s) 985
BstX2I RGATCY 2 cut(s) 328, 937
BstYI RGATCY 2 cut(s) 328, 937
BsuRI GGCC 3 cut(s) 176, 320, 1264
BtsCI GGATG 2 cut(s) 507, 1432
BtsI GCAGTG 2 cut(s) 659, 1068
BtsIMutI CAGTG 5 cut(s) 321, 659, 1068, 1386, 1449
BveI ACCTGC 2 cut(s) 1097, 1316
Cac8I GCNNGC 4 cut(s) 15, 879, 1221, 1266
CfoI GCGC 2 cut(s) 384, 1219
Cfr13I GGNCC 2 cut(s) 114, 1127
CseI GACGC 2 cut(s) 20, 1455
CviAII CATG 5 cut(s) 271, 811, 1097, 1199, 1220
DdeI CTNAG 4 cut(s) 110, 474, 594, 1347
DpnI GATC 6 cut(s) 330, 672, 681, 891, 932, 939
DpnII GATC 6 cut(s) 328, 670, 679, 889, 930, 937
DraI TTTAAA 1 cut(s) 496
DrdI GACNNNNNNGTC 1 cut(s) 158
DriI GACNNNNNGTC 1 cut(s) 1058
DseDI GACNNNNNNGTC 1 cut(s) 158
EaeI YGGCCR 1 cut(s) 174
Eam1104I CTCTTC 4 cut(s) 401, 864, 921, 968
Eam1105I GACNNNNNGTC 1 cut(s) 1058
EarI CTCTTC 4 cut(s) 401, 864, 921, 968
Eco130I CCWWGG 1 cut(s) 1422
Eco24I GRGCYC 2 cut(s) 351, 720
Eco31I GGTCTC 2 cut(s) 159, 233
Eco47I GGWCC 2 cut(s) 114, 1127
Eco47III AGCGCT 1 cut(s) 383
EcoNI CCTNNNNNAGG 1 cut(s) 1151
EcoO109I RGGNCCY 1 cut(s) 114
EcoRI GAATTC 1 cut(s) 800
EcoRII CCWGG 1 cut(s) 1274
EcoT14I CCWWGG 1 cut(s) 1422
EcoT38I GRGCYC 2 cut(s) 351, 720
ErhI CCWWGG 1 cut(s) 1422
Esp3I CGTCTC 1 cut(s) 38
FaeI CATG 5 cut(s) 274, 814, 1100, 1202, 1223
FaqI GGGAC 2 cut(s) 1121, 1450
FatI CATG 5 cut(s) 270, 810, 1096, 1198, 1219
FauI CCCGC 1 cut(s) 71
FauNDI CATATG 1 cut(s) 1159
Fnu4HI GCNGC 2 cut(s) 620, 650
FokI GGATG 2 cut(s) 514, 1439
FriOI GRGCYC 2 cut(s) 351, 720
Fsp4HI GCNGC 2 cut(s) 620, 650
FspBI CTAG 4 cut(s) 734, 878, 920, 941
FspI TGCGCA 1 cut(s) 1218
GlaI GCGC 2 cut(s) 383, 1218
GluI GCNGC 2 cut(s) 620, 650
GsuI CTGGAG 2 cut(s) 734, 1133
HaeII RGCGCY 1 cut(s) 385
HaeIII GGCC 3 cut(s) 176, 320, 1264
HapII CCGG 2 cut(s) 538, 1027
HgaI GACGC 2 cut(s) 20, 1455
HhaI GCGC 2 cut(s) 384, 1219
Hin1II CATG 5 cut(s) 274, 814, 1100, 1202, 1223
Hin6I GCGC 2 cut(s) 382, 1217
HinP1I GCGC 2 cut(s) 382, 1217
HindIII AAGCTT 1 cut(s) 296
HinfI GANTC 5 cut(s) 356, 558, 696, 1177, 1366
HpaII CCGG 2 cut(s) 538, 1027
HphI GGTGA 1 cut(s) 578
Hpy166II GTNNAC 2 cut(s) 435, 1024
Hpy188I TCNGA 6 cut(s) 151, 164, 361, 426, 935, 1176
Hpy8I GTNNAC 2 cut(s) 435, 1024
Hpy99I CGWCG 3 cut(s) 374, 377, 380
HpyAV CCTTC 8 cut(s) 91, 179, 298, 652, 808, 901, 1147, 1378
HpyCH4III ACNGT 5 cut(s) 145, 581, 1303, 1381, 1453
HpyCH4V TGCA 5 cut(s) 619, 652, 1106, 1223, 1268
HpyF10VI GCNNNNNNNGC 4 cut(s) 538, 649, 1112, 1406
HpyF3I CTNAG 4 cut(s) 110, 474, 594, 1347
Hsp92II CATG 5 cut(s) 274, 814, 1100, 1202, 1223
HspAI GCGC 2 cut(s) 382, 1217
Kzo9I GATC 6 cut(s) 328, 670, 679, 889, 930, 937
LmnI GCTCC 3 cut(s) 346, 715, 1204
Lsp1109I GCAGC 2 cut(s) 631, 636
LweI GCATC 3 cut(s) 385, 478, 730
MaeI CTAG 4 cut(s) 734, 878, 920, 941
MaeIII GTNAC 3 cut(s) 277, 365, 1039
MalI GATC 6 cut(s) 330, 672, 681, 891, 932, 939
MboI GATC 6 cut(s) 328, 670, 679, 889, 930, 937
MfeI CAATTG 1 cut(s) 1212
MflI RGATCY 2 cut(s) 328, 937
MhlI GDGCHC 2 cut(s) 351, 720
MlyI GAGTC 2 cut(s) 567, 705
MmeI TCCRAC 1 cut(s) 867
MroXI GAANNNNTTC 2 cut(s) 998, 1311
MseI TTAA 3 cut(s) 495, 1013, 1404
MspA1I CMGCKG 1 cut(s) 1133
MspI CCGG 2 cut(s) 538, 1027
MspR9I CCNGG 1 cut(s) 1276
MunI CAATTG 1 cut(s) 1212
MvaI CCWGG 1 cut(s) 1276
MvnI CGCG 2 cut(s) 31, 439
MwoI GCNNNNNNNGC 4 cut(s) 538, 649, 1112, 1406
NdeI CATATG 1 cut(s) 1159
NdeII GATC 6 cut(s) 328, 670, 679, 889, 930, 937
NheI GCTAGC 1 cut(s) 877
NlaIII CATG 5 cut(s) 274, 814, 1100, 1202, 1223
NlaIV GGNNCC 2 cut(s) 348, 717
NmeAIII GCCGAG 1 cut(s) 202
NmuCI GTSAC 2 cut(s) 277, 365
NsbI TGCGCA 1 cut(s) 1218
NspI RCATGY 2 cut(s) 274, 1223
PaeI GCATGC 1 cut(s) 1223
PcsI WCGNNNNNNNCGW 1 cut(s) 375
PdmI GAANNNNTTC 2 cut(s) 998, 1311
PfeI GAWTC 3 cut(s) 356, 1177, 1366
PkrI GCNGC 2 cut(s) 621, 651
PleI GAGTC 2 cut(s) 566, 704
PpsI GAGTC 2 cut(s) 566, 704
PpuMI RGGWCCY 1 cut(s) 114
PshBI ATTAAT 1 cut(s) 1013
Psp5II RGGWCCY 1 cut(s) 114
Psp6I CCWGG 1 cut(s) 1274
PspGI CCWGG 1 cut(s) 1274
PspN4I GGNNCC 2 cut(s) 348, 717
PspPI GGNCC 2 cut(s) 114, 1127
PspPPI RGGWCCY 1 cut(s) 114
PstI CTGCAG 2 cut(s) 621, 654
PsuI RGATCY 2 cut(s) 328, 937
PvuII CAGCTG 1 cut(s) 1133
SaqAI TTAA 3 cut(s) 495, 1013, 1404
SatI GCNGC 2 cut(s) 620, 650
Sau3AI GATC 6 cut(s) 328, 670, 679, 889, 930, 937
Sau96I GGNCC 2 cut(s) 114, 1127
SchI GAGTC 2 cut(s) 567, 705
ScrFI CCNGG 1 cut(s) 1276
SduI GDGCHC 2 cut(s) 351, 720
SfaNI GCATC 3 cut(s) 385, 478, 730
SfcI CTRYAG 3 cut(s) 617, 650, 988
SinI GGWCC 2 cut(s) 114, 1127
SmlI CTYRAG 3 cut(s) 299, 314, 455
SmoI CTYRAG 3 cut(s) 299, 314, 455
SphI GCATGC 1 cut(s) 1223
SsiI CCGC 1 cut(s) 64
SspMI CTAG 4 cut(s) 734, 878, 920, 941
StyD4I CCNGG 1 cut(s) 1274
StyI CCWWGG 1 cut(s) 1422
TaaI ACNGT 5 cut(s) 145, 581, 1303, 1381, 1453
TaqI TCGA 5 cut(s) 183, 231, 772, 1002, 1305
TfiI GAWTC 3 cut(s) 356, 1177, 1366
Tru1I TTAA 3 cut(s) 495, 1013, 1404
Tru9I TTAA 3 cut(s) 495, 1013, 1404
TscAI CASTG 5 cut(s) 328, 659, 1075, 1386, 1456
TseFI GTSAC 2 cut(s) 277, 365
TseI GCWGC 2 cut(s) 619, 649
Tsp45I GTSAC 2 cut(s) 277, 365
TspDTI ATGAA 7 cut(s) 73, 518, 590, 601, 810, 827, 1301
TspGWI ACGGA 1 cut(s) 980
TspRI CASTG 5 cut(s) 328, 659, 1075, 1386, 1456
VpaK11BI GGWCC 2 cut(s) 114, 1127
VspI ATTAAT 1 cut(s) 1013
XagI CCTNNNNNAGG 1 cut(s) 1151
XapI RAATTY 3 cut(s) 460, 608, 800
XbaI TCTAGA 2 cut(s) 733, 919
XceI RCATGY 2 cut(s) 274, 1223
XcmI CCANNNNNNNNNTGG 1 cut(s) 1318
XmnI GAANNNNTTC 2 cut(s) 998, 1311
XspI CTAG 4 cut(s) 734, 878, 920, 941
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.