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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AgsI | TTSAA | 1 cut(s) | 39 |
| AluBI | AGCT | 1 cut(s) | 168 |
| AluI | AGCT | 1 cut(s) | 168 |
| AsuHPI | GGTGA | 4 cut(s) | 20, 71, 125, 140 |
| BbsI | GAAGAC | 1 cut(s) | 164 |
| BccI | CCATC | 3 cut(s) | 22, 73, 143 |
| BmsI | GCATC | 1 cut(s) | 109 |
| BpiI | GAAGAC | 1 cut(s) | 164 |
| Bse3DI | GCAATG | 1 cut(s) | 129 |
| BseGI | GGATG | 2 cut(s) | 33, 84 |
| BseMI | GCAATG | 1 cut(s) | 129 |
| BseRI | GAGGAG | 1 cut(s) | 123 |
| BsrDI | GCAATG | 1 cut(s) | 129 |
| BstC8I | GCNNGC | 2 cut(s) | 49, 100 |
| BstF5I | GGATG | 2 cut(s) | 33, 84 |
| BstV2I | GAAGAC | 1 cut(s) | 164 |
| BtgZI | GCGATG | 1 cut(s) | 159 |
| BtsCI | GGATG | 2 cut(s) | 33, 84 |
| Cac8I | GCNNGC | 2 cut(s) | 49, 100 |
| CviAII | CATG | 2 cut(s) | 52, 103 |
| CviJI | RGCY | 1 cut(s) | 168 |
| CviKI_1 | RGCY | 1 cut(s) | 168 |
| FaeI | CATG | 2 cut(s) | 55, 106 |
| FaiI | YATR | 2 cut(s) | 53, 104 |
| FatI | CATG | 2 cut(s) | 51, 102 |
| FokI | GGATG | 2 cut(s) | 40, 91 |
| Hin1II | CATG | 2 cut(s) | 55, 106 |
| HphI | GGTGA | 4 cut(s) | 20, 71, 125, 140 |
| Hpy188III | TCNNGA | 1 cut(s) | 88 |
| HpyAV | CCTTC | 2 cut(s) | 5, 56 |
| HpyCH4V | TGCA | 1 cut(s) | 122 |
| Hsp92II | CATG | 2 cut(s) | 55, 106 |
| LpnPI | CCDG | 2 cut(s) | 33, 84 |
| LweI | GCATC | 1 cut(s) | 109 |
| MaeIII | GTNAC | 1 cut(s) | 128 |
| MboII | GAAGA | 1 cut(s) | 169 |
| MnlI | CCTC | 3 cut(s) | 50, 101, 104 |
| MseI | TTAA | 1 cut(s) | 170 |
| NlaIII | CATG | 2 cut(s) | 55, 106 |
| NmuCI | GTSAC | 1 cut(s) | 128 |
| SaqAI | TTAA | 1 cut(s) | 170 |
| SetI | ASST | 6 cut(s) | 10, 16, 61, 67, 115, 170 |
| SfaNI | GCATC | 1 cut(s) | 109 |
| SgeI | CNNG | 7 cut(s) | 56, 60, 64, 100, 107, 111, 115 |
| TaqI | TCGA | 1 cut(s) | 89 |
| Tru1I | TTAA | 1 cut(s) | 170 |
| Tru9I | TTAA | 1 cut(s) | 170 |
| TseFI | GTSAC | 1 cut(s) | 128 |
| Tsp45I | GTSAC | 1 cut(s) | 128 |