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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 103 |
| AccII | CGCG | 1 cut(s) | 33 |
| AciI | CCGC | 3 cut(s) | 12, 23, 31 |
| AcuI | CTGAAG | 1 cut(s) | 144 |
| AfaI | GTAC | 1 cut(s) | 175 |
| AgsI | TTSAA | 1 cut(s) | 297 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 104 |
| AoxI | GGCC | 2 cut(s) | 245, 317 |
| AspLEI | GCGC | 1 cut(s) | 17 |
| AspS9I | GGNCC | 1 cut(s) | 317 |
| AsuHPI | GGTGA | 1 cut(s) | 293 |
| BfmI | CTRYAG | 1 cut(s) | 96 |
| BfuAI | ACCTGC | 1 cut(s) | 103 |
| BisI | GCNGC | 1 cut(s) | 13 |
| BlsI | GCNGC | 1 cut(s) | 14 |
| BmgT120I | GGNCC | 1 cut(s) | 317 |
| BmiI | GGNNCC | 2 cut(s) | 254, 318 |
| BsaJI | CCNNGG | 1 cut(s) | 248 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 128, 158 |
| Bse3DI | GCAATG | 1 cut(s) | 214 |
| BseDI | CCNNGG | 1 cut(s) | 248 |
| BseGI | GGATG | 1 cut(s) | 152 |
| BseMI | GCAATG | 1 cut(s) | 214 |
| BseMII | CTCAG | 1 cut(s) | 320 |
| Bsh1236I | CGCG | 1 cut(s) | 33 |
| BshFI | GGCC | 2 cut(s) | 247, 319 |
| BsnI | GGCC | 2 cut(s) | 247, 319 |
| BspACI | CCGC | 3 cut(s) | 12, 23, 31 |
| BspANI | GGCC | 2 cut(s) | 247, 319 |
| BspCNI | CTCAG | 1 cut(s) | 319 |
| BspFNI | CGCG | 1 cut(s) | 33 |
| BspLI | GGNNCC | 2 cut(s) | 254, 318 |
| BspMAI | CTGCAG | 1 cut(s) | 100 |
| BspMI | ACCTGC | 1 cut(s) | 103 |
| BsrDI | GCAATG | 1 cut(s) | 214 |
| BssECI | CCNNGG | 1 cut(s) | 248 |
| BssT1I | CCWWGG | 1 cut(s) | 248 |
| Bst4CI | ACNGT | 3 cut(s) | 44, 106, 257 |
| Bst6I | CTCTTC | 1 cut(s) | 163 |
| BstDEI | CTNAG | 1 cut(s) | 306 |
| BstF5I | GGATG | 1 cut(s) | 152 |
| BstFNI | CGCG | 1 cut(s) | 33 |
| BstHHI | GCGC | 1 cut(s) | 17 |
| BstSFI | CTRYAG | 1 cut(s) | 96 |
| BstUI | CGCG | 1 cut(s) | 33 |
| BsuRI | GGCC | 2 cut(s) | 247, 319 |
| BtsCI | GGATG | 1 cut(s) | 152 |
| BveI | ACCTGC | 1 cut(s) | 103 |
| CaiI | CAGNNNCTG | 1 cut(s) | 104 |
| CfoI | GCGC | 1 cut(s) | 17 |
| Cfr13I | GGNCC | 1 cut(s) | 317 |
| Csp6I | GTAC | 1 cut(s) | 174 |
| CviAII | CATG | 3 cut(s) | 83, 200, 341 |
| CviJI | RGCY | 2 cut(s) | 247, 319 |
| CviKI_1 | RGCY | 2 cut(s) | 247, 319 |
| CviQI | GTAC | 1 cut(s) | 174 |
| DdeI | CTNAG | 1 cut(s) | 306 |
| Eam1104I | CTCTTC | 1 cut(s) | 163 |
| EarI | CTCTTC | 1 cut(s) | 163 |
| EciI | GGCGGA | 1 cut(s) | 38 |
| Eco130I | CCWWGG | 1 cut(s) | 248 |
| Eco147I | AGGCCT | 1 cut(s) | 247 |
| Eco57I | CTGAAG | 1 cut(s) | 144 |
| EcoO109I | RGGNCCY | 1 cut(s) | 317 |
| EcoT14I | CCWWGG | 1 cut(s) | 248 |
| ErhI | CCWWGG | 1 cut(s) | 248 |
| FaeI | CATG | 3 cut(s) | 86, 203, 344 |
| FaiI | YATR | 5 cut(s) | 84, 92, 179, 201, 342 |
| FatI | CATG | 3 cut(s) | 82, 199, 340 |
| FauI | CCCGC | 1 cut(s) | 38 |
| Fnu4HI | GCNGC | 1 cut(s) | 13 |
| FokI | GGATG | 1 cut(s) | 139 |
| Fsp4HI | GCNGC | 1 cut(s) | 13 |
| GlaI | GCGC | 1 cut(s) | 16 |
| GluI | GCNGC | 1 cut(s) | 13 |
| HaeIII | GGCC | 2 cut(s) | 247, 319 |
| HhaI | GCGC | 1 cut(s) | 17 |
| Hin1II | CATG | 3 cut(s) | 86, 203, 344 |
| Hin6I | GCGC | 1 cut(s) | 15 |
| HinP1I | GCGC | 1 cut(s) | 15 |
| HincII | GTYRAC | 1 cut(s) | 79 |
| HindII | GTYRAC | 1 cut(s) | 79 |
| HinfI | GANTC | 1 cut(s) | 293 |
| HphI | GGTGA | 1 cut(s) | 293 |
| Hpy166II | GTNNAC | 1 cut(s) | 79 |
| Hpy188I | TCNGA | 3 cut(s) | 163, 184, 309 |
| Hpy8I | GTNNAC | 1 cut(s) | 79 |
| HpyCH4III | ACNGT | 3 cut(s) | 44, 106, 257 |
| HpyCH4IV | ACGT | 1 cut(s) | 172 |
| HpyCH4V | TGCA | 1 cut(s) | 98 |
| HpyF3I | CTNAG | 1 cut(s) | 306 |
| HpySE526I | ACGT | 1 cut(s) | 172 |
| Hsp92II | CATG | 3 cut(s) | 86, 203, 344 |
| HspAI | GCGC | 1 cut(s) | 15 |
| LmnI | GCTCC | 1 cut(s) | 136 |
| LpnPI | CCDG | 5 cut(s) | 108, 111, 125, 245, 299 |
| MaeII | ACGT | 1 cut(s) | 172 |
| MaeIII | GTNAC | 1 cut(s) | 108 |
| MboII | GAAGA | 2 cut(s) | 150, 281 |
| MnlI | CCTC | 5 cut(s) | 133, 166, 259, 271, 330 |
| MseI | TTAA | 2 cut(s) | 39, 345 |
| MslI | CAYNNNNRTG | 1 cut(s) | 345 |
| MvnI | CGCG | 1 cut(s) | 33 |
| NlaIII | CATG | 3 cut(s) | 86, 203, 344 |
| NlaIV | GGNNCC | 2 cut(s) | 254, 318 |
| PceI | AGGCCT | 1 cut(s) | 247 |
| PfeI | GAWTC | 1 cut(s) | 293 |
| PkrI | GCNGC | 1 cut(s) | 14 |
| PspN4I | GGNNCC | 2 cut(s) | 254, 318 |
| PspPI | GGNCC | 1 cut(s) | 317 |
| PstI | CTGCAG | 1 cut(s) | 100 |
| PstNI | CAGNNNCTG | 1 cut(s) | 104 |
| RsaI | GTAC | 1 cut(s) | 175 |
| RsaNI | GTAC | 1 cut(s) | 174 |
| RseI | CAYNNNNRTG | 1 cut(s) | 345 |
| SaqAI | TTAA | 2 cut(s) | 39, 345 |
| SatI | GCNGC | 1 cut(s) | 13 |
| Sau96I | GGNCC | 1 cut(s) | 317 |
| SetI | ASST | 2 cut(s) | 97, 175 |
| SfcI | CTRYAG | 1 cut(s) | 96 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 345 |
| SseBI | AGGCCT | 1 cut(s) | 247 |
| SsiI | CCGC | 3 cut(s) | 12, 23, 31 |
| StuI | AGGCCT | 1 cut(s) | 247 |
| StyI | CCWWGG | 1 cut(s) | 248 |
| TaaI | ACNGT | 3 cut(s) | 44, 106, 257 |
| TaiI | ACGT | 1 cut(s) | 175 |
| TauI | GCSGC | 1 cut(s) | 15 |
| TfiI | GAWTC | 1 cut(s) | 293 |
| Tru1I | TTAA | 2 cut(s) | 39, 345 |
| Tru9I | TTAA | 2 cut(s) | 39, 345 |
| TspDTI | ATGAA | 3 cut(s) | 81, 183, 222 |