Rroxscaffold_4G00327060

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0002163
Physical Location & Seq
Reverse (-)
59500727 .. 59505850
5124 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00327060.1

Sequence Viewer

Length: 453 bp
ATGGTGACTGTCCCTTTCTTGAAGCGCTTGAAGAAAGCCATGACCACAAAAGGACGGGAAGCACGATCGCGAGTGCCCGACGATAAGAATAATCCCCGTGTCTTGGGGGGAAAGCATGTTATTACGGAGCTGAACGGTGGCTCCACCCACCTGAGTTTCAATTCATATCAAACACGTCTTTCTTCCGAATACCCGGAAGAATTGTACAACCTAATTCTCATCCCGGATTCATTCCCTCCGCAATCGCCGCTCTCCCCTCCGACGCCGTCGTCGTCCACCTCTTGCGCCCTATCCGGAAAGGTAACAGACAAAGCAAGCTCTAGGAAATTTGTAGTTGAGAGCATGTTTTGGACTTTCTCGATGCATCATACAAGTCTGGCTGCATATTGTTATTCTACTTGTGGAGGTCTACTTTGTGCTTACATAATTTACTTGCAGCAAATTTTTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

150

Amino Acids

16.64

Weight (kDa)

9.25

Isoelectric Point (pI)

54.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 268
AccBSI CCGCTC 1 cut(s) 250
AccI GTMKAC 1 cut(s) 409
AccII CGCG 1 cut(s) 70
AccIII TCCGGA 1 cut(s) 293
AciI CCGC 2 cut(s) 239, 248
AcsI RAATTY 2 cut(s) 326, 441
AcyI GRCGYC 1 cut(s) 263
AfaI GTAC 1 cut(s) 206
AfeI AGCGCT 1 cut(s) 26
AfiI CCNNNNNNNGG 1 cut(s) 103
AflIII ACRYGT 1 cut(s) 173
AgsI TTSAA 3 cut(s) 22, 31, 160
AjiI CACGTC 1 cut(s) 176
AloI GAACNNNNNNTCC 2 cut(s) 125, 157
AluBI AGCT 2 cut(s) 130, 318
AluI AGCT 2 cut(s) 130, 318
Aor13HI TCCGGA 1 cut(s) 293
Aor51HI AGCGCT 1 cut(s) 26
ApeKI GCWGC 2 cut(s) 380, 436
ApoI RAATTY 2 cut(s) 326, 441
AspLEI GCGC 2 cut(s) 27, 287
AsuC2I CCSGG 2 cut(s) 194, 224
AsuHPI GGTGA 1 cut(s) 16
BaeGI GKGCMC 1 cut(s) 78
BbvI GCAGC 2 cut(s) 367, 448
BceAI ACGGC 1 cut(s) 250
BcnI CCSGG 2 cut(s) 194, 224
BfaI CTAG 1 cut(s) 321
BfoI RGCGCY 1 cut(s) 28
BisI GCNGC 3 cut(s) 248, 381, 437
BlsI GCNGC 3 cut(s) 249, 382, 438
Bme1390I CCNGG 2 cut(s) 194, 224
BmgBI CACGTC 1 cut(s) 176
BmiI GGNNCC 1 cut(s) 142
BmrFI CCNGG 2 cut(s) 194, 224
BmsI GCATC 2 cut(s) 351, 373
BpuMI CCSGG 2 cut(s) 194, 224
BsaHI GRCGYC 1 cut(s) 263
BsaWI WCCGGW 1 cut(s) 293
BsaXI ACNNNNNCTCC 2 cut(s) 125, 155
Bsc4I CCNNNNNNNGG 1 cut(s) 103
BseAI TCCGGA 1 cut(s) 293
BseGI GGATG 1 cut(s) 219
BseLI CCNNNNNNNGG 1 cut(s) 103
BseMII CTCAG 1 cut(s) 143
BseSI GKGCMC 1 cut(s) 78
BseXI GCAGC 2 cut(s) 367, 448
Bsh1236I CGCG 1 cut(s) 70
Bsh1285I CGRYCG 1 cut(s) 68
BsiEI CGRYCG 1 cut(s) 68
BsiSI CCGG 3 cut(s) 194, 224, 294
BslI CCNNNNNNNGG 1 cut(s) 103
Bsp1286I GDGCHC 1 cut(s) 78
Bsp13I TCCGGA 1 cut(s) 293
Bsp1407I TGTACA 1 cut(s) 204
Bsp143I GATC 1 cut(s) 65
Bsp68I TCGCGA 1 cut(s) 70
BspACI CCGC 2 cut(s) 239, 248
BspCNI CTCAG 1 cut(s) 144
BspEI TCCGGA 1 cut(s) 293
BspFNI CGCG 1 cut(s) 70
BspLI GGNNCC 1 cut(s) 142
BsrBI CCGCTC 1 cut(s) 250
BsrGI TGTACA 1 cut(s) 204
BssMI GATC 1 cut(s) 65
BssNI GRCGYC 1 cut(s) 263
Bst4CI ACNGT 2 cut(s) 10, 137
BstACI GRCGYC 1 cut(s) 263
BstAUI TGTACA 1 cut(s) 204
BstC8I GCNNGC 1 cut(s) 316
BstDEI CTNAG 2 cut(s) 152, 450
BstF5I GGATG 1 cut(s) 219
BstFNI CGCG 1 cut(s) 70
BstH2I RGCGCY 1 cut(s) 28
BstHHI GCGC 2 cut(s) 27, 287
BstKTI GATC 1 cut(s) 68
BstMBI GATC 1 cut(s) 65
BstMCI CGRYCG 1 cut(s) 68
BstMWI GCNNNNNNNGC 1 cut(s) 247
BstNSI RCATGY 2 cut(s) 119, 346
BstSCI CCNGG 2 cut(s) 192, 222
BstSLI GKGCMC 1 cut(s) 78
BstUI CGCG 1 cut(s) 70
BstV1I GCAGC 2 cut(s) 367, 448
BtrI CACGTC 1 cut(s) 176
BtsCI GGATG 1 cut(s) 219
BtuMI TCGCGA 1 cut(s) 70
Cac8I GCNNGC 1 cut(s) 316
CfoI GCGC 2 cut(s) 27, 287
CseI GACGC 1 cut(s) 271
Csp6I GTAC 1 cut(s) 205
CviAII CATG 3 cut(s) 40, 116, 343
CviJI RGCY 5 cut(s) 38, 130, 141, 318, 380
CviKI_1 RGCY 5 cut(s) 38, 130, 141, 318, 380
CviQI GTAC 1 cut(s) 205
DdeI CTNAG 2 cut(s) 152, 450
DpnI GATC 1 cut(s) 67
DpnII GATC 1 cut(s) 65
DrdI GACNNNNNNGTC 1 cut(s) 268
DseDI GACNNNNNNGTC 1 cut(s) 268
Eco47III AGCGCT 1 cut(s) 26
EcoT22I ATGCAT 1 cut(s) 366
FaeI CATG 3 cut(s) 43, 119, 346
FaiI YATR 7 cut(s) 41, 117, 166, 344, 369, 385, 425
FatI CATG 3 cut(s) 39, 115, 342
FblI GTMKAC 1 cut(s) 409
Fnu4HI GCNGC 3 cut(s) 248, 381, 437
FokI GGATG 1 cut(s) 206
Fsp4HI GCNGC 3 cut(s) 248, 381, 437
FspBI CTAG 1 cut(s) 321
GlaI GCGC 2 cut(s) 26, 286
GluI GCNGC 3 cut(s) 248, 381, 437
HaeII RGCGCY 1 cut(s) 28
HapII CCGG 3 cut(s) 194, 224, 294
HgaI GACGC 1 cut(s) 271
HhaI GCGC 2 cut(s) 27, 287
Hin1I GRCGYC 1 cut(s) 263
Hin1II CATG 3 cut(s) 43, 119, 346
Hin6I GCGC 2 cut(s) 25, 285
HinP1I GCGC 2 cut(s) 25, 285
HinfI GANTC 1 cut(s) 227
HpaII CCGG 3 cut(s) 194, 224, 294
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 2 cut(s) 276, 410
Hpy188I TCNGA 2 cut(s) 187, 261
Hpy188III TCNNGA 4 cut(s) 19, 69, 294, 358
Hpy8I GTNNAC 2 cut(s) 276, 410
Hpy99I CGWCG 4 cut(s) 83, 265, 271, 274
HpyCH4III ACNGT 2 cut(s) 10, 137
HpyCH4IV ACGT 1 cut(s) 175
HpyCH4V TGCA 3 cut(s) 364, 383, 436
HpyF10VI GCNNNNNNNGC 1 cut(s) 247
HpyF3I CTNAG 2 cut(s) 152, 450
HpySE526I ACGT 1 cut(s) 175
Hsp92I GRCGYC 1 cut(s) 263
Hsp92II CATG 3 cut(s) 43, 119, 346
HspAI GCGC 2 cut(s) 25, 285
Kpn2I TCCGGA 1 cut(s) 293
Kzo9I GATC 1 cut(s) 65
LmnI GCTCC 2 cut(s) 127, 146
LpnPI CCDG 5 cut(s) 164, 207, 237, 307, 362
Lsp1109I GCAGC 2 cut(s) 367, 448
LweI GCATC 2 cut(s) 351, 373
MaeI CTAG 1 cut(s) 321
MaeII ACGT 1 cut(s) 175
MaeIII GTNAC 2 cut(s) 4, 301
MalI GATC 1 cut(s) 67
MbiI CCGCTC 1 cut(s) 250
MboI GATC 1 cut(s) 65
MboII GAAGA 3 cut(s) 43, 174, 209
MhlI GDGCHC 1 cut(s) 78
MluCI AATT 6 cut(s) 160, 200, 213, 326, 426, 441
MmeI TCCRAC 1 cut(s) 284
MnlI CCTC 4 cut(s) 246, 267, 289, 398
Mph1103I ATGCAT 1 cut(s) 366
MroI TCCGGA 1 cut(s) 293
MspI CCGG 3 cut(s) 194, 224, 294
MspR9I CCNGG 2 cut(s) 194, 224
MvnI CGCG 1 cut(s) 70
MwoI GCNNNNNNNGC 1 cut(s) 247
NciI CCSGG 2 cut(s) 194, 224
NdeII GATC 1 cut(s) 65
NlaIII CATG 3 cut(s) 43, 119, 346
NlaIV GGNNCC 1 cut(s) 142
NmuCI GTSAC 1 cut(s) 4
NruI TCGCGA 1 cut(s) 70
NsiI ATGCAT 1 cut(s) 366
NspI RCATGY 2 cut(s) 119, 346
PcsI WCGNNNNNNNCGW 2 cut(s) 61, 269
PfeI GAWTC 1 cut(s) 227
PflFI GACNNNGTC 1 cut(s) 265
PfoI TCCNGGA 1 cut(s) 222
PkrI GCNGC 3 cut(s) 249, 382, 438
Ple19I CGATCG 1 cut(s) 68
PspN4I GGNNCC 1 cut(s) 142
PsyI GACNNNGTC 1 cut(s) 265
PvuI CGATCG 1 cut(s) 68
RruI TCGCGA 1 cut(s) 70
RsaI GTAC 1 cut(s) 206
RsaNI GTAC 1 cut(s) 205
SatI GCNGC 3 cut(s) 248, 381, 437
Sau3AI GATC 1 cut(s) 65
ScrFI CCNGG 2 cut(s) 194, 224
SduI GDGCHC 1 cut(s) 78
SetI ASST 8 cut(s) 132, 153, 178, 213, 281, 303, 320, 409
SfaNI GCATC 2 cut(s) 351, 373
Sse9I AATT 6 cut(s) 160, 200, 213, 326, 426, 441
SsiI CCGC 2 cut(s) 239, 248
SspMI CTAG 1 cut(s) 321
StyD4I CCNGG 2 cut(s) 192, 222
TaaI ACNGT 2 cut(s) 10, 137
TaiI ACGT 1 cut(s) 178
TaqI TCGA 1 cut(s) 359
TasI AATT 6 cut(s) 160, 200, 213, 326, 426, 441
TatI WGTACW 1 cut(s) 204
TauI GCSGC 1 cut(s) 250
TfiI GAWTC 1 cut(s) 227
TseFI GTSAC 1 cut(s) 4
TseI GCWGC 2 cut(s) 380, 436
Tsp45I GTSAC 1 cut(s) 4
TspDTI ATGAA 2 cut(s) 153, 219
TspGWI ACGGA 1 cut(s) 140
Tth111I GACNNNGTC 1 cut(s) 265
XapI RAATTY 2 cut(s) 326, 441
XceI RCATGY 2 cut(s) 119, 346
XmiI GTMKAC 1 cut(s) 409
XspI CTAG 1 cut(s) 321
Zsp2I ATGCAT 1 cut(s) 366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.