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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 134 |
| AccIII | TCCGGA | 1 cut(s) | 81 |
| AciI | CCGC | 3 cut(s) | 94, 129, 132 |
| AcsI | RAATTY | 1 cut(s) | 16 |
| AcyI | GRCGYC | 1 cut(s) | 148 |
| AflIII | ACRYGT | 1 cut(s) | 30 |
| AgsI | TTSAA | 1 cut(s) | 236 |
| AjiI | CACGTC | 1 cut(s) | 33 |
| AluBI | AGCT | 2 cut(s) | 142, 365 |
| AluI | AGCT | 2 cut(s) | 142, 365 |
| Alw26I | GTCTC | 1 cut(s) | 94 |
| Aor13HI | TCCGGA | 1 cut(s) | 81 |
| ApoI | RAATTY | 1 cut(s) | 16 |
| AsuHPI | GGTGA | 4 cut(s) | 217, 268, 322, 337 |
| BbsI | GAAGAC | 2 cut(s) | 27, 361 |
| BccI | CCATC | 3 cut(s) | 219, 270, 340 |
| BceAI | ACGGC | 1 cut(s) | 135 |
| BcoDI | GTCTC | 1 cut(s) | 94 |
| BisI | GCNGC | 2 cut(s) | 129, 132 |
| BlsI | GCNGC | 2 cut(s) | 130, 133 |
| BmgBI | CACGTC | 1 cut(s) | 33 |
| BmrI | ACTGGG | 1 cut(s) | 64 |
| BmsI | GCATC | 1 cut(s) | 306 |
| BmuI | ACTGGG | 1 cut(s) | 64 |
| BpiI | GAAGAC | 2 cut(s) | 27, 361 |
| BpmI | CTGGAG | 1 cut(s) | 122 |
| BsaHI | GRCGYC | 1 cut(s) | 148 |
| BsaI | GGTCTC | 1 cut(s) | 94 |
| BsaWI | WCCGGW | 1 cut(s) | 81 |
| Bse1I | ACTGG | 1 cut(s) | 70 |
| Bse3DI | GCAATG | 1 cut(s) | 326 |
| BseAI | TCCGGA | 1 cut(s) | 81 |
| BseGI | GGATG | 2 cut(s) | 230, 281 |
| BseMI | GCAATG | 1 cut(s) | 326 |
| BseNI | ACTGG | 1 cut(s) | 70 |
| BseRI | GAGGAG | 1 cut(s) | 320 |
| BsiSI | CCGG | 1 cut(s) | 82 |
| BsmAI | GTCTC | 1 cut(s) | 94 |
| Bso31I | GGTCTC | 1 cut(s) | 94 |
| Bsp13I | TCCGGA | 1 cut(s) | 81 |
| BspACI | CCGC | 3 cut(s) | 94, 129, 132 |
| BspEI | TCCGGA | 1 cut(s) | 81 |
| BspTNI | GGTCTC | 1 cut(s) | 94 |
| BsrBI | CCGCTC | 1 cut(s) | 134 |
| BsrDI | GCAATG | 1 cut(s) | 326 |
| BsrI | ACTGG | 1 cut(s) | 70 |
| BssNI | GRCGYC | 1 cut(s) | 148 |
| Bst6I | CTCTTC | 1 cut(s) | 45 |
| BstACI | GRCGYC | 1 cut(s) | 148 |
| BstC8I | GCNNGC | 2 cut(s) | 246, 297 |
| BstF5I | GGATG | 2 cut(s) | 230, 281 |
| BstMAI | GTCTC | 1 cut(s) | 94 |
| BstV2I | GAAGAC | 2 cut(s) | 27, 361 |
| BtgZI | GCGATG | 1 cut(s) | 356 |
| BtrI | CACGTC | 1 cut(s) | 33 |
| BtsCI | GGATG | 2 cut(s) | 230, 281 |
| Cac8I | GCNNGC | 2 cut(s) | 246, 297 |
| CseI | GACGC | 1 cut(s) | 156 |
| CviAII | CATG | 4 cut(s) | 8, 198, 249, 300 |
| CviJI | RGCY | 3 cut(s) | 116, 142, 365 |
| CviKI_1 | RGCY | 3 cut(s) | 116, 142, 365 |
| Eam1104I | CTCTTC | 1 cut(s) | 45 |
| EarI | CTCTTC | 1 cut(s) | 45 |
| Eco31I | GGTCTC | 1 cut(s) | 94 |
| Eco32I | GATATC | 1 cut(s) | 24 |
| EcoRV | GATATC | 1 cut(s) | 24 |
| FaeI | CATG | 4 cut(s) | 11, 201, 252, 303 |
| FaiI | YATR | 4 cut(s) | 9, 199, 250, 301 |
| FatI | CATG | 4 cut(s) | 7, 197, 248, 299 |
| FauI | CCCGC | 1 cut(s) | 101 |
| Fnu4HI | GCNGC | 2 cut(s) | 129, 132 |
| FokI | GGATG | 2 cut(s) | 237, 288 |
| Fsp4HI | GCNGC | 2 cut(s) | 129, 132 |
| GluI | GCNGC | 2 cut(s) | 129, 132 |
| GsuI | CTGGAG | 1 cut(s) | 122 |
| HapII | CCGG | 1 cut(s) | 82 |
| HgaI | GACGC | 1 cut(s) | 156 |
| Hin1I | GRCGYC | 1 cut(s) | 148 |
| Hin1II | CATG | 4 cut(s) | 11, 201, 252, 303 |
| HinfI | GANTC | 1 cut(s) | 85 |
| HpaII | CCGG | 1 cut(s) | 82 |
| HphI | GGTGA | 4 cut(s) | 217, 268, 322, 337 |
| Hpy188I | TCNGA | 1 cut(s) | 146 |
| Hpy188III | TCNNGA | 4 cut(s) | 13, 82, 179, 285 |
| Hpy99I | CGWCG | 2 cut(s) | 150, 156 |
| HpyAV | CCTTC | 3 cut(s) | 127, 202, 253 |
| HpyCH4IV | ACGT | 1 cut(s) | 32 |
| HpyCH4V | TGCA | 2 cut(s) | 63, 319 |
| HpySE526I | ACGT | 1 cut(s) | 32 |
| Hsp92I | GRCGYC | 1 cut(s) | 148 |
| Hsp92II | CATG | 4 cut(s) | 11, 201, 252, 303 |
| Kpn2I | TCCGGA | 1 cut(s) | 81 |
| LmnI | GCTCC | 1 cut(s) | 147 |
| LpnPI | CCDG | 8 cut(s) | 65, 83, 95, 110, 152, 164, 230, 281 |
| LweI | GCATC | 1 cut(s) | 306 |
| MaeII | ACGT | 1 cut(s) | 32 |
| MaeIII | GTNAC | 1 cut(s) | 325 |
| MbiI | CCGCTC | 1 cut(s) | 134 |
| MboII | GAAGA | 4 cut(s) | 27, 32, 67, 366 |
| MluCI | AATT | 2 cut(s) | 16, 58 |
| MmeI | TCCRAC | 1 cut(s) | 169 |
| MnlI | CCTC | 5 cut(s) | 184, 196, 247, 298, 301 |
| MroI | TCCGGA | 1 cut(s) | 81 |
| MseI | TTAA | 1 cut(s) | 367 |
| MspI | CCGG | 1 cut(s) | 82 |
| NlaIII | CATG | 4 cut(s) | 11, 201, 252, 303 |
| NmuCI | GTSAC | 1 cut(s) | 325 |
| PfeI | GAWTC | 1 cut(s) | 85 |
| PflFI | GACNNNGTC | 1 cut(s) | 150 |
| PkrI | GCNGC | 2 cut(s) | 130, 133 |
| PsyI | GACNNNGTC | 1 cut(s) | 150 |
| SaqAI | TTAA | 1 cut(s) | 367 |
| SatI | GCNGC | 2 cut(s) | 129, 132 |
| SetI | ASST | 9 cut(s) | 35, 144, 169, 207, 213, 258, 264, 312, 367 |
| SfaNI | GCATC | 1 cut(s) | 306 |
| Sse9I | AATT | 2 cut(s) | 16, 58 |
| SsiI | CCGC | 3 cut(s) | 94, 129, 132 |
| TaiI | ACGT | 1 cut(s) | 35 |
| TaqI | TCGA | 1 cut(s) | 286 |
| TasI | AATT | 2 cut(s) | 16, 58 |
| TauI | GCSGC | 2 cut(s) | 131, 134 |
| TfiI | GAWTC | 1 cut(s) | 85 |
| Tru1I | TTAA | 1 cut(s) | 367 |
| Tru9I | TTAA | 1 cut(s) | 367 |
| TseFI | GTSAC | 1 cut(s) | 325 |
| Tsp45I | GTSAC | 1 cut(s) | 325 |
| TspDTI | ATGAA | 1 cut(s) | 17 |
| Tth111I | GACNNNGTC | 1 cut(s) | 150 |
| XapI | RAATTY | 1 cut(s) | 16 |