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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 1 cut(s) | 265 |
| AcsI | RAATTY | 1 cut(s) | 170 |
| AgsI | TTSAA | 2 cut(s) | 29, 47 |
| AluBI | AGCT | 1 cut(s) | 162 |
| AluI | AGCT | 1 cut(s) | 162 |
| AlwI | GGATC | 1 cut(s) | 265 |
| ApoI | RAATTY | 1 cut(s) | 170 |
| AseI | ATTAAT | 1 cut(s) | 9 |
| BfaI | CTAG | 2 cut(s) | 72, 165 |
| BmsI | GCATC | 1 cut(s) | 195 |
| Bsp143I | GATC | 1 cut(s) | 257 |
| BspPI | GGATC | 1 cut(s) | 265 |
| BssMI | GATC | 1 cut(s) | 257 |
| BstC8I | GCNNGC | 1 cut(s) | 160 |
| BstKTI | GATC | 1 cut(s) | 260 |
| BstMBI | GATC | 1 cut(s) | 257 |
| BstNSI | RCATGY | 1 cut(s) | 190 |
| BstX2I | RGATCY | 1 cut(s) | 257 |
| BstYI | RGATCY | 1 cut(s) | 257 |
| BtsIMutI | CAGTG | 1 cut(s) | 268 |
| Cac8I | GCNNGC | 1 cut(s) | 160 |
| CviAII | CATG | 1 cut(s) | 187 |
| CviJI | RGCY | 2 cut(s) | 113, 162 |
| CviKI_1 | RGCY | 2 cut(s) | 113, 162 |
| DpnI | GATC | 1 cut(s) | 259 |
| DpnII | GATC | 1 cut(s) | 257 |
| FaeI | CATG | 1 cut(s) | 190 |
| FaiI | YATR | 4 cut(s) | 17, 188, 213, 229 |
| FatI | CATG | 1 cut(s) | 186 |
| FspBI | CTAG | 2 cut(s) | 72, 165 |
| Hin1II | CATG | 1 cut(s) | 190 |
| HincII | GTYRAC | 1 cut(s) | 100 |
| HindII | GTYRAC | 1 cut(s) | 100 |
| Hpy166II | GTNNAC | 1 cut(s) | 100 |
| Hpy188I | TCNGA | 1 cut(s) | 79 |
| Hpy188III | TCNNGA | 1 cut(s) | 202 |
| Hpy8I | GTNNAC | 1 cut(s) | 100 |
| HpyCH4V | TGCA | 2 cut(s) | 4, 227 |
| Hsp92II | CATG | 1 cut(s) | 190 |
| Kzo9I | GATC | 1 cut(s) | 257 |
| LpnPI | CCDG | 1 cut(s) | 206 |
| LweI | GCATC | 1 cut(s) | 195 |
| MaeI | CTAG | 2 cut(s) | 72, 165 |
| MaeIII | GTNAC | 3 cut(s) | 35, 145, 266 |
| MalI | GATC | 1 cut(s) | 259 |
| MboI | GATC | 1 cut(s) | 257 |
| MboII | GAAGA | 3 cut(s) | 124, 127, 130 |
| MflI | RGATCY | 1 cut(s) | 257 |
| MluCI | AATT | 3 cut(s) | 6, 10, 170 |
| MnlI | CCTC | 2 cut(s) | 242, 247 |
| MseI | TTAA | 1 cut(s) | 9 |
| NdeII | GATC | 1 cut(s) | 257 |
| NlaIII | CATG | 1 cut(s) | 190 |
| NmuCI | GTSAC | 1 cut(s) | 266 |
| NspI | RCATGY | 1 cut(s) | 190 |
| PshBI | ATTAAT | 1 cut(s) | 9 |
| PsuI | RGATCY | 1 cut(s) | 257 |
| SaqAI | TTAA | 1 cut(s) | 9 |
| Sau3AI | GATC | 1 cut(s) | 257 |
| SetI | ASST | 3 cut(s) | 37, 77, 164 |
| SfaNI | GCATC | 1 cut(s) | 195 |
| SgeI | CNNG | 9 cut(s) | 84, 154, 171, 177, 199, 214, 228, 233, 255 |
| Sse9I | AATT | 3 cut(s) | 6, 10, 170 |
| SspMI | CTAG | 2 cut(s) | 72, 165 |
| TaqI | TCGA | 2 cut(s) | 52, 203 |
| TasI | AATT | 3 cut(s) | 6, 10, 170 |
| Tru1I | TTAA | 1 cut(s) | 9 |
| Tru9I | TTAA | 1 cut(s) | 9 |
| TseFI | GTSAC | 1 cut(s) | 266 |
| Tsp45I | GTSAC | 1 cut(s) | 266 |
| TspDTI | ATGAA | 1 cut(s) | 200 |
| VspI | ATTAAT | 1 cut(s) | 9 |
| XapI | RAATTY | 1 cut(s) | 170 |
| XceI | RCATGY | 1 cut(s) | 190 |
| XspI | CTAG | 2 cut(s) | 72, 165 |