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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 208 |
| AcsI | RAATTY | 2 cut(s) | 87, 130 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 289 |
| AflIII | ACRYGT | 1 cut(s) | 144 |
| AgsI | TTSAA | 6 cut(s) | 51, 93, 315, 347, 376, 390 |
| AjiI | CACGTC | 2 cut(s) | 57, 147 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 73, 105 |
| AluBI | AGCT | 3 cut(s) | 279, 305, 311 |
| AluI | AGCT | 3 cut(s) | 279, 305, 311 |
| Alw26I | GTCTC | 1 cut(s) | 339 |
| ApoI | RAATTY | 2 cut(s) | 87, 130 |
| AsuC2I | CCSGG | 1 cut(s) | 196 |
| AsuHPI | GGTGA | 1 cut(s) | 304 |
| BbsI | GAAGAC | 2 cut(s) | 51, 141 |
| BccI | CCATC | 1 cut(s) | 244 |
| BceAI | ACGGC | 1 cut(s) | 195 |
| BcnI | CCSGG | 1 cut(s) | 196 |
| BcoDI | GTCTC | 1 cut(s) | 339 |
| BfmI | CTRYAG | 1 cut(s) | 270 |
| Bme1390I | CCNGG | 1 cut(s) | 196 |
| BmgBI | CACGTC | 2 cut(s) | 57, 147 |
| BmiI | GGNNCC | 1 cut(s) | 119 |
| BmrFI | CCNGG | 1 cut(s) | 196 |
| BmrI | ACTGGG | 1 cut(s) | 178 |
| BmuI | ACTGGG | 1 cut(s) | 178 |
| BpiI | GAAGAC | 2 cut(s) | 51, 141 |
| BpuMI | CCSGG | 1 cut(s) | 196 |
| BsaI | GGTCTC | 1 cut(s) | 339 |
| BsaWI | WCCGGW | 1 cut(s) | 108 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 46, 76 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 289 |
| Bse1I | ACTGG | 1 cut(s) | 184 |
| BseGI | GGATG | 1 cut(s) | 191 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 289 |
| BseNI | ACTGG | 1 cut(s) | 184 |
| BseRI | GAGGAG | 1 cut(s) | 54 |
| Bsh1285I | CGRYCG | 1 cut(s) | 113 |
| BsiEI | CGRYCG | 1 cut(s) | 113 |
| BsiSI | CCGG | 2 cut(s) | 109, 196 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 289 |
| BsmAI | GTCTC | 1 cut(s) | 339 |
| Bso31I | GGTCTC | 1 cut(s) | 339 |
| BspACI | CCGC | 1 cut(s) | 208 |
| BspLI | GGNNCC | 1 cut(s) | 119 |
| BspTNI | GGTCTC | 1 cut(s) | 339 |
| BsrI | ACTGG | 1 cut(s) | 184 |
| Bst6I | CTCTTC | 3 cut(s) | 72, 159, 391 |
| BstF5I | GGATG | 1 cut(s) | 191 |
| BstMAI | GTCTC | 1 cut(s) | 339 |
| BstMCI | CGRYCG | 1 cut(s) | 113 |
| BstSCI | CCNGG | 1 cut(s) | 194 |
| BstSFI | CTRYAG | 1 cut(s) | 270 |
| BstV2I | GAAGAC | 2 cut(s) | 51, 141 |
| BtgZI | GCGATG | 1 cut(s) | 222 |
| BtrI | CACGTC | 2 cut(s) | 57, 147 |
| BtsCI | GGATG | 1 cut(s) | 191 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 303, 338 |
| CviAII | CATG | 1 cut(s) | 122 |
| CviJI | RGCY | 3 cut(s) | 279, 305, 311 |
| CviKI_1 | RGCY | 3 cut(s) | 279, 305, 311 |
| Eam1104I | CTCTTC | 3 cut(s) | 72, 159, 391 |
| EarI | CTCTTC | 3 cut(s) | 72, 159, 391 |
| Eco31I | GGTCTC | 1 cut(s) | 339 |
| FaeI | CATG | 1 cut(s) | 125 |
| FaiI | YATR | 2 cut(s) | 123, 397 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 356, 388 |
| FatI | CATG | 1 cut(s) | 121 |
| FauI | CCCGC | 1 cut(s) | 215 |
| FokI | GGATG | 1 cut(s) | 178 |
| HapII | CCGG | 2 cut(s) | 109, 196 |
| Hin1II | CATG | 1 cut(s) | 125 |
| HindIII | AAGCTT | 1 cut(s) | 309 |
| HinfI | GANTC | 3 cut(s) | 199, 266, 383 |
| HpaII | CCGG | 2 cut(s) | 109, 196 |
| HphI | GGTGA | 1 cut(s) | 304 |
| Hpy188III | TCNNGA | 2 cut(s) | 127, 373 |
| Hpy99I | CGWCG | 1 cut(s) | 216 |
| HpyAV | CCTTC | 1 cut(s) | 321 |
| HpyCH4IV | ACGT | 2 cut(s) | 56, 146 |
| HpyCH4V | TGCA | 1 cut(s) | 177 |
| HpySE526I | ACGT | 2 cut(s) | 56, 146 |
| Hsp92II | CATG | 1 cut(s) | 125 |
| LmnI | GCTCC | 2 cut(s) | 246, 284 |
| LpnPI | CCDG | 5 cut(s) | 122, 179, 197, 209, 275 |
| MaeII | ACGT | 2 cut(s) | 56, 146 |
| MboII | GAAGA | 6 cut(s) | 30, 51, 59, 141, 146, 378 |
| MfeI | CAATTG | 1 cut(s) | 172 |
| MluCI | AATT | 4 cut(s) | 45, 87, 130, 172 |
| MlyI | GAGTC | 1 cut(s) | 377 |
| MnlI | CCTC | 5 cut(s) | 75, 237, 261, 324, 373 |
| MseI | TTAA | 2 cut(s) | 9, 44 |
| MspI | CCGG | 2 cut(s) | 109, 196 |
| MspR9I | CCNGG | 1 cut(s) | 196 |
| MunI | CAATTG | 1 cut(s) | 172 |
| NciI | CCSGG | 1 cut(s) | 196 |
| NlaIII | CATG | 1 cut(s) | 125 |
| NlaIV | GGNNCC | 1 cut(s) | 119 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 208 |
| PfeI | GAWTC | 2 cut(s) | 199, 266 |
| PfoI | TCCNGGA | 1 cut(s) | 194 |
| PleI | GAGTC | 1 cut(s) | 377 |
| PpsI | GAGTC | 1 cut(s) | 377 |
| PspN4I | GGNNCC | 1 cut(s) | 119 |
| SaqAI | TTAA | 2 cut(s) | 9, 44 |
| SchI | GAGTC | 1 cut(s) | 377 |
| ScrFI | CCNGG | 1 cut(s) | 196 |
| SetI | ASST | 9 cut(s) | 59, 149, 229, 253, 281, 307, 313, 320, 335 |
| SfcI | CTRYAG | 1 cut(s) | 270 |
| Sse9I | AATT | 4 cut(s) | 45, 87, 130, 172 |
| SsiI | CCGC | 1 cut(s) | 208 |
| StyD4I | CCNGG | 1 cut(s) | 194 |
| TaiI | ACGT | 2 cut(s) | 59, 149 |
| TasI | AATT | 4 cut(s) | 45, 87, 130, 172 |
| TfiI | GAWTC | 2 cut(s) | 199, 266 |
| Tru1I | TTAA | 2 cut(s) | 9, 44 |
| Tru9I | TTAA | 2 cut(s) | 9, 44 |
| TspDTI | ATGAA | 2 cut(s) | 358, 365 |
| XapI | RAATTY | 2 cut(s) | 87, 130 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 255 |