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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 155 |
| AccII | CGCG | 1 cut(s) | 234 |
| AccIII | TCCGGA | 1 cut(s) | 102 |
| AciI | CCGC | 2 cut(s) | 150, 153 |
| AcsI | RAATTY | 3 cut(s) | 16, 37, 322 |
| AfaI | GTAC | 1 cut(s) | 412 |
| AflIII | ACRYGT | 2 cut(s) | 51, 232 |
| AgsI | TTSAA | 1 cut(s) | 279 |
| AjiI | CACGTC | 1 cut(s) | 54 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 384, 416 |
| AluBI | AGCT | 2 cut(s) | 163, 185 |
| AluI | AGCT | 2 cut(s) | 163, 185 |
| Alw26I | GTCTC | 1 cut(s) | 115 |
| Aor13HI | TCCGGA | 1 cut(s) | 102 |
| ApoI | RAATTY | 3 cut(s) | 16, 37, 322 |
| AsuHPI | GGTGA | 5 cut(s) | 139, 260, 311, 365, 380 |
| BbsI | GAAGAC | 1 cut(s) | 48 |
| BccI | CCATC | 1 cut(s) | 262 |
| BcoDI | GTCTC | 1 cut(s) | 115 |
| BisI | GCNGC | 1 cut(s) | 153 |
| BlsI | GCNGC | 1 cut(s) | 154 |
| BmgBI | CACGTC | 1 cut(s) | 54 |
| BmrI | ACTGGG | 1 cut(s) | 85 |
| BmsI | GCATC | 1 cut(s) | 349 |
| BmuI | ACTGGG | 1 cut(s) | 85 |
| BpiI | GAAGAC | 1 cut(s) | 48 |
| BpmI | CTGGAG | 2 cut(s) | 143, 165 |
| BsaI | GGTCTC | 1 cut(s) | 115 |
| BsaWI | WCCGGW | 1 cut(s) | 102 |
| Bse1I | ACTGG | 1 cut(s) | 91 |
| Bse3DI | GCAATG | 1 cut(s) | 369 |
| BseAI | TCCGGA | 1 cut(s) | 102 |
| BseGI | GGATG | 2 cut(s) | 273, 324 |
| BseMI | GCAATG | 1 cut(s) | 369 |
| BseNI | ACTGG | 1 cut(s) | 91 |
| BseRI | GAGGAG | 1 cut(s) | 363 |
| Bsh1236I | CGCG | 1 cut(s) | 234 |
| BsiSI | CCGG | 1 cut(s) | 103 |
| BsmAI | GTCTC | 1 cut(s) | 115 |
| BsmI | GAATGC | 1 cut(s) | 436 |
| Bso31I | GGTCTC | 1 cut(s) | 115 |
| Bsp13I | TCCGGA | 1 cut(s) | 102 |
| Bsp1407I | TGTACA | 1 cut(s) | 410 |
| BspACI | CCGC | 2 cut(s) | 150, 153 |
| BspEI | TCCGGA | 1 cut(s) | 102 |
| BspFNI | CGCG | 1 cut(s) | 234 |
| BspTNI | GGTCTC | 1 cut(s) | 115 |
| BsrBI | CCGCTC | 1 cut(s) | 155 |
| BsrDI | GCAATG | 1 cut(s) | 369 |
| BsrGI | TGTACA | 1 cut(s) | 410 |
| BsrI | ACTGG | 1 cut(s) | 91 |
| Bst4CI | ACNGT | 1 cut(s) | 173 |
| Bst6I | CTCTTC | 1 cut(s) | 66 |
| BstAUI | TGTACA | 1 cut(s) | 410 |
| BstC8I | GCNNGC | 2 cut(s) | 289, 340 |
| BstEII | GGTNACC | 1 cut(s) | 145 |
| BstF5I | GGATG | 2 cut(s) | 273, 324 |
| BstFNI | CGCG | 1 cut(s) | 234 |
| BstMAI | GTCTC | 1 cut(s) | 115 |
| BstPI | GGTNACC | 1 cut(s) | 145 |
| BstUI | CGCG | 1 cut(s) | 234 |
| BstV2I | GAAGAC | 1 cut(s) | 48 |
| BtgZI | GCGATG | 1 cut(s) | 399 |
| BtrI | CACGTC | 1 cut(s) | 54 |
| BtsCI | GGATG | 2 cut(s) | 273, 324 |
| Cac8I | GCNNGC | 2 cut(s) | 289, 340 |
| Csp6I | GTAC | 1 cut(s) | 411 |
| CviAII | CATG | 6 cut(s) | 8, 29, 241, 292, 343, 433 |
| CviJI | RGCY | 3 cut(s) | 137, 163, 185 |
| CviKI_1 | RGCY | 3 cut(s) | 137, 163, 185 |
| CviQI | GTAC | 1 cut(s) | 411 |
| Eam1104I | CTCTTC | 1 cut(s) | 66 |
| EarI | CTCTTC | 1 cut(s) | 66 |
| Eco31I | GGTCTC | 1 cut(s) | 115 |
| Eco32I | GATATC | 1 cut(s) | 45 |
| Eco91I | GGTNACC | 1 cut(s) | 145 |
| EcoO65I | GGTNACC | 1 cut(s) | 145 |
| EcoRV | GATATC | 1 cut(s) | 45 |
| EcoT22I | ATGCAT | 1 cut(s) | 438 |
| FaeI | CATG | 6 cut(s) | 11, 32, 244, 295, 346, 436 |
| FaiI | YATR | 6 cut(s) | 9, 30, 242, 293, 344, 434 |
| FatI | CATG | 6 cut(s) | 7, 28, 240, 291, 342, 432 |
| Fnu4HI | GCNGC | 1 cut(s) | 153 |
| FokI | GGATG | 2 cut(s) | 280, 331 |
| Fsp4HI | GCNGC | 1 cut(s) | 153 |
| GluI | GCNGC | 1 cut(s) | 153 |
| GsuI | CTGGAG | 2 cut(s) | 143, 165 |
| HapII | CCGG | 1 cut(s) | 103 |
| Hin1II | CATG | 6 cut(s) | 11, 32, 244, 295, 346, 436 |
| HinfI | GANTC | 1 cut(s) | 106 |
| HpaII | CCGG | 1 cut(s) | 103 |
| HphI | GGTGA | 5 cut(s) | 139, 260, 311, 365, 380 |
| Hpy188I | TCNGA | 1 cut(s) | 167 |
| Hpy188III | TCNNGA | 5 cut(s) | 13, 34, 73, 103, 222 |
| Hpy99I | CGWCG | 1 cut(s) | 177 |
| HpyAV | CCTTC | 3 cut(s) | 148, 245, 296 |
| HpyCH4III | ACNGT | 1 cut(s) | 173 |
| HpyCH4IV | ACGT | 1 cut(s) | 53 |
| HpyCH4V | TGCA | 3 cut(s) | 84, 362, 436 |
| HpySE526I | ACGT | 1 cut(s) | 53 |
| Hsp92II | CATG | 6 cut(s) | 11, 32, 244, 295, 346, 436 |
| Kpn2I | TCCGGA | 1 cut(s) | 102 |
| LmnI | GCTCC | 2 cut(s) | 168, 190 |
| LpnPI | CCDG | 9 cut(s) | 86, 104, 116, 131, 173, 195, 207, 273, 324 |
| LweI | GCATC | 1 cut(s) | 349 |
| MaeII | ACGT | 1 cut(s) | 53 |
| MaeIII | GTNAC | 2 cut(s) | 145, 368 |
| MbiI | CCGCTC | 1 cut(s) | 155 |
| MboII | GAAGA | 4 cut(s) | 48, 53, 88, 417 |
| MluCI | AATT | 5 cut(s) | 16, 37, 79, 322, 399 |
| MluI | ACGCGT | 1 cut(s) | 232 |
| MmeI | TCCRAC | 2 cut(s) | 190, 212 |
| MnlI | CCTC | 5 cut(s) | 227, 239, 290, 341, 344 |
| Mph1103I | ATGCAT | 1 cut(s) | 438 |
| MroI | TCCGGA | 1 cut(s) | 102 |
| MspI | CCGG | 1 cut(s) | 103 |
| Mva1269I | GAATGC | 1 cut(s) | 436 |
| MvnI | CGCG | 1 cut(s) | 234 |
| NlaIII | CATG | 6 cut(s) | 11, 32, 244, 295, 346, 436 |
| NmuCI | GTSAC | 2 cut(s) | 145, 368 |
| NsiI | ATGCAT | 1 cut(s) | 438 |
| PctI | GAATGC | 1 cut(s) | 436 |
| PfeI | GAWTC | 1 cut(s) | 106 |
| PflFI | GACNNNGTC | 1 cut(s) | 171 |
| PkrI | GCNGC | 1 cut(s) | 154 |
| PspEI | GGTNACC | 1 cut(s) | 145 |
| PsyI | GACNNNGTC | 1 cut(s) | 171 |
| RsaI | GTAC | 1 cut(s) | 412 |
| RsaNI | GTAC | 1 cut(s) | 411 |
| SatI | GCNGC | 1 cut(s) | 153 |
| SetI | ASST | 9 cut(s) | 56, 165, 187, 212, 250, 256, 301, 307, 355 |
| SfaNI | GCATC | 1 cut(s) | 349 |
| Sse9I | AATT | 5 cut(s) | 16, 37, 79, 322, 399 |
| SsiI | CCGC | 2 cut(s) | 150, 153 |
| TaaI | ACNGT | 1 cut(s) | 173 |
| TaiI | ACGT | 1 cut(s) | 56 |
| TasI | AATT | 5 cut(s) | 16, 37, 79, 322, 399 |
| TatI | WGTACW | 1 cut(s) | 410 |
| TauI | GCSGC | 1 cut(s) | 155 |
| TfiI | GAWTC | 1 cut(s) | 106 |
| TseFI | GTSAC | 2 cut(s) | 145, 368 |
| Tsp45I | GTSAC | 2 cut(s) | 145, 368 |
| TspDTI | ATGAA | 4 cut(s) | 17, 335, 405, 421 |
| Tth111I | GACNNNGTC | 1 cut(s) | 171 |
| XapI | RAATTY | 3 cut(s) | 16, 37, 322 |
| Zsp2I | ATGCAT | 1 cut(s) | 438 |