Rh1AG166100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0002163
Physical Location & Seq
Forward (+)
31646733 .. 31664764
18032 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG166100.1

Sequence Viewer

Length: 471 bp
ATGGCAGATCCGGCCTACCAAACTTTCTCACTGGTCGCTGTTGGGAGCAACATTTCAATCAGTTCTGGTTATGAGTTAAAGAAACTTAGGTCCTTACTGCAGGGTGTTTGGCAACATTATTCGATCGGGTCGTTCCACGGCTTCTCCTCTTCTCACAGTTCCAAAAAAATTTTCAGAACCCAAATCCTTCCCTCCAATGAACCATGTCAAGAATTTGACAACCCATGTCAAGAATTTGATATCAAACACGTCTTCTCTTCTCAATACCCAGAAGAATTGCACAACCCAGTTCTCATTCCGGATTCGTTCCCGCCAGAGACCCAAAATAATATCCTCCGCGGTCCCCGCCGCTCTCCACCACCTCCTACACCGTCGCGCTCCACCTCCTACGCCCTTTTTGGAAAGAAACTCGCTGGCATGGGAGGTGAAGGTGGAATGGGTGGGATGGATTTCTCAGTTAGTATATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

156

Amino Acids

17.2

Weight (kDa)

6.96

Isoelectric Point (pI)

67.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 351
AccII CGCG 2 cut(s) 339, 376
AccIII TCCGGA 1 cut(s) 298
AciI CCGC 5 cut(s) 311, 337, 339, 346, 349
AclWI GGATC 1 cut(s) 2
AcsI RAATTY 3 cut(s) 168, 212, 233
AflIII ACRYGT 1 cut(s) 247
AgsI TTSAA 1 cut(s) 57
AjiI CACGTC 1 cut(s) 250
AjuI GAANNNNNNNTTGG 2 cut(s) 155, 187
Alw26I GTCTC 1 cut(s) 311
AlwI GGATC 1 cut(s) 2
Aor13HI TCCGGA 1 cut(s) 298
AoxI GGCC 1 cut(s) 12
ApoI RAATTY 3 cut(s) 168, 212, 233
AspLEI GCGC 1 cut(s) 378
AspS9I GGNCC 2 cut(s) 90, 341
AsuHPI GGTGA 1 cut(s) 437
AvaII GGWCC 2 cut(s) 90, 341
BbsI GAAGAC 1 cut(s) 244
BccI CCATC 1 cut(s) 439
BceAI ACGGC 1 cut(s) 154
BcoDI GTCTC 1 cut(s) 311
BfmI CTRYAG 1 cut(s) 98
BisI GCNGC 1 cut(s) 349
BlsI GCNGC 1 cut(s) 350
Bme18I GGWCC 2 cut(s) 90, 341
BmgBI CACGTC 1 cut(s) 250
BmgT120I GGNCC 2 cut(s) 90, 341
BmiI GGNNCC 1 cut(s) 343
BmrI ACTGGG 1 cut(s) 281
BmuI ACTGGG 1 cut(s) 281
BpiI GAAGAC 1 cut(s) 244
BsaI GGTCTC 1 cut(s) 311
BsaJI CCNNGG 2 cut(s) 136, 337
BsaWI WCCGGW 1 cut(s) 298
BsaXI ACNNNNNCTCC 2 cut(s) 128, 158
Bse1I ACTGG 2 cut(s) 36, 287
BseAI TCCGGA 1 cut(s) 298
BseDI CCNNGG 2 cut(s) 136, 337
BseGI GGATG 1 cut(s) 450
BseMII CTCAG 1 cut(s) 468
BseNI ACTGG 2 cut(s) 36, 287
BseRI GAGGAG 1 cut(s) 136
Bsh1236I CGCG 2 cut(s) 339, 376
Bsh1285I CGRYCG 1 cut(s) 126
BshFI GGCC 1 cut(s) 14
BsiEI CGRYCG 1 cut(s) 126
BsiSI CCGG 2 cut(s) 11, 299
BslFI GGGAC 1 cut(s) 327
BsmAI GTCTC 1 cut(s) 311
BsmFI GGGAC 1 cut(s) 327
BsnI GGCC 1 cut(s) 14
Bso31I GGTCTC 1 cut(s) 311
Bsp13I TCCGGA 1 cut(s) 298
Bsp143I GATC 2 cut(s) 7, 123
BspACI CCGC 5 cut(s) 311, 337, 339, 346, 349
BspANI GGCC 1 cut(s) 14
BspCNI CTCAG 1 cut(s) 467
BspEI TCCGGA 1 cut(s) 298
BspFNI CGCG 2 cut(s) 339, 376
BspLI GGNNCC 1 cut(s) 343
BspMAI CTGCAG 1 cut(s) 102
BspPI GGATC 1 cut(s) 2
BspTNI GGTCTC 1 cut(s) 311
BsrBI CCGCTC 1 cut(s) 351
BsrI ACTGG 2 cut(s) 36, 287
BssECI CCNNGG 2 cut(s) 136, 337
BssMI GATC 2 cut(s) 7, 123
Bst4CI ACNGT 2 cut(s) 158, 372
Bst6I CTCTTC 2 cut(s) 154, 262
BstC8I GCNNGC 1 cut(s) 415
BstDEI CTNAG 2 cut(s) 86, 454
BstDSI CCRYGG 2 cut(s) 136, 337
BstF5I GGATG 1 cut(s) 450
BstFNI CGCG 2 cut(s) 339, 376
BstHHI GCGC 1 cut(s) 378
BstKTI GATC 2 cut(s) 10, 126
BstMAI GTCTC 1 cut(s) 311
BstMBI GATC 2 cut(s) 7, 123
BstMCI CGRYCG 1 cut(s) 126
BstMWI GCNNNNNNNGC 2 cut(s) 11, 345
BstSFI CTRYAG 1 cut(s) 98
BstUI CGCG 2 cut(s) 339, 376
BstV2I GAAGAC 1 cut(s) 244
BstX2I RGATCY 1 cut(s) 7
BstYI RGATCY 1 cut(s) 7
BsuRI GGCC 1 cut(s) 14
BtgI CCRYGG 2 cut(s) 136, 337
BtrI CACGTC 1 cut(s) 250
BtsCI GGATG 1 cut(s) 450
BtsIMutI CAGTG 1 cut(s) 29
Cac8I GCNNGC 1 cut(s) 415
CfoI GCGC 1 cut(s) 378
Cfr13I GGNCC 2 cut(s) 90, 341
Cfr42I CCGCGG 1 cut(s) 340
CviAII CATG 3 cut(s) 204, 225, 418
CviJI RGCY 2 cut(s) 14, 141
CviKI_1 RGCY 2 cut(s) 14, 141
DdeI CTNAG 2 cut(s) 86, 454
DpnI GATC 2 cut(s) 9, 125
DpnII GATC 2 cut(s) 7, 123
Eam1104I CTCTTC 2 cut(s) 154, 262
EarI CTCTTC 2 cut(s) 154, 262
Eco31I GGTCTC 1 cut(s) 311
Eco32I GATATC 1 cut(s) 241
Eco47I GGWCC 2 cut(s) 90, 341
EcoO109I RGGNCCY 1 cut(s) 90
EcoRV GATATC 1 cut(s) 241
FaeI CATG 3 cut(s) 207, 228, 421
FaiI YATR 6 cut(s) 72, 205, 226, 419, 464, 466
FaqI GGGAC 1 cut(s) 327
FatI CATG 3 cut(s) 203, 224, 417
FauI CCCGC 2 cut(s) 318, 353
Fnu4HI GCNGC 1 cut(s) 349
FokI GGATG 1 cut(s) 457
Fsp4HI GCNGC 1 cut(s) 349
GlaI GCGC 1 cut(s) 377
GluI GCNGC 1 cut(s) 349
HaeIII GGCC 1 cut(s) 14
HapII CCGG 2 cut(s) 11, 299
HhaI GCGC 1 cut(s) 378
Hin1II CATG 3 cut(s) 207, 228, 421
Hin6I GCGC 1 cut(s) 376
HinP1I GCGC 1 cut(s) 376
HinfI GANTC 1 cut(s) 302
HpaII CCGG 2 cut(s) 11, 299
HphI GGTGA 1 cut(s) 437
Hpy188I TCNGA 1 cut(s) 176
Hpy188III TCNNGA 3 cut(s) 209, 230, 299
Hpy99I CGWCG 1 cut(s) 376
HpyAV CCTTC 2 cut(s) 197, 422
HpyCH4III ACNGT 2 cut(s) 158, 372
HpyCH4IV ACGT 1 cut(s) 249
HpyCH4V TGCA 2 cut(s) 100, 280
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 345
HpyF3I CTNAG 2 cut(s) 86, 454
HpySE526I ACGT 1 cut(s) 249
Hsp92II CATG 3 cut(s) 207, 228, 421
HspAI GCGC 1 cut(s) 376
Kpn2I TCCGGA 1 cut(s) 298
KspI CCGCGG 1 cut(s) 340
Kzo9I GATC 2 cut(s) 7, 123
LmnI GCTCC 2 cut(s) 45, 383
LpnPI CCDG 9 cut(s) 17, 24, 51, 86, 282, 300, 312, 327, 399
MaeII ACGT 1 cut(s) 249
MalI GATC 2 cut(s) 9, 125
MbiI CCGCTC 1 cut(s) 351
MboI GATC 2 cut(s) 7, 123
MboII GAAGA 4 cut(s) 141, 244, 249, 284
MflI RGATCY 1 cut(s) 7
MluCI AATT 4 cut(s) 168, 212, 233, 275
MnlI CCTC 6 cut(s) 157, 202, 344, 372, 394, 416
MroI TCCGGA 1 cut(s) 298
MseI TTAA 1 cut(s) 77
MspA1I CMGCKG 1 cut(s) 339
MspI CCGG 2 cut(s) 11, 299
MvnI CGCG 2 cut(s) 339, 376
MwoI GCNNNNNNNGC 2 cut(s) 11, 345
NdeII GATC 2 cut(s) 7, 123
NlaIII CATG 3 cut(s) 207, 228, 421
NlaIV GGNNCC 1 cut(s) 343
PcsI WCGNNNNNNNCGW 1 cut(s) 128
PfeI GAWTC 1 cut(s) 302
PkrI GCNGC 1 cut(s) 350
Ple19I CGATCG 1 cut(s) 126
PpuMI RGGWCCY 1 cut(s) 90
Psp5II RGGWCCY 1 cut(s) 90
PspN4I GGNNCC 1 cut(s) 343
PspPI GGNCC 2 cut(s) 90, 341
PspPPI RGGWCCY 1 cut(s) 90
PstI CTGCAG 1 cut(s) 102
PsuI RGATCY 1 cut(s) 7
PvuI CGATCG 1 cut(s) 126
SacII CCGCGG 1 cut(s) 340
SaqAI TTAA 1 cut(s) 77
SatI GCNGC 1 cut(s) 349
Sau3AI GATC 2 cut(s) 7, 123
Sau96I GGNCC 2 cut(s) 90, 341
SetI ASST 6 cut(s) 92, 252, 364, 386, 427, 433
SfcI CTRYAG 1 cut(s) 98
Sfr303I CCGCGG 1 cut(s) 340
SgrBI CCGCGG 1 cut(s) 340
SinI GGWCC 2 cut(s) 90, 341
Sse9I AATT 4 cut(s) 168, 212, 233, 275
SsiI CCGC 5 cut(s) 311, 337, 339, 346, 349
TaaI ACNGT 2 cut(s) 158, 372
TaiI ACGT 1 cut(s) 252
TaqI TCGA 1 cut(s) 122
TasI AATT 4 cut(s) 168, 212, 233, 275
TauI GCSGC 1 cut(s) 351
TfiI GAWTC 1 cut(s) 302
Tru1I TTAA 1 cut(s) 77
Tru9I TTAA 1 cut(s) 77
TscAI CASTG 1 cut(s) 36
TspDTI ATGAA 1 cut(s) 213
TspRI CASTG 1 cut(s) 36
VpaK11BI GGWCC 2 cut(s) 90, 341
XapI RAATTY 3 cut(s) 168, 212, 233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.