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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 44 |
| AciI | CCGC | 2 cut(s) | 39, 42 |
| AcyI | GRCGYC | 2 cut(s) | 58, 80 |
| AgsI | TTSAA | 1 cut(s) | 171 |
| AluBI | AGCT | 2 cut(s) | 52, 74 |
| AluI | AGCT | 2 cut(s) | 52, 74 |
| AsuHPI | GGTGA | 4 cut(s) | 152, 203, 257, 272 |
| AsuNHI | GCTAGC | 1 cut(s) | 230 |
| BccI | CCATC | 3 cut(s) | 91, 154, 205 |
| BceAI | ACGGC | 1 cut(s) | 45 |
| BfaI | CTAG | 1 cut(s) | 231 |
| BisI | GCNGC | 2 cut(s) | 39, 42 |
| BlsI | GCNGC | 2 cut(s) | 40, 43 |
| BmsI | GCATC | 1 cut(s) | 241 |
| BmtI | GCTAGC | 1 cut(s) | 234 |
| BpmI | CTGGAG | 2 cut(s) | 32, 54 |
| BsaHI | GRCGYC | 2 cut(s) | 58, 80 |
| Bse3DI | GCAATG | 1 cut(s) | 261 |
| BseGI | GGATG | 2 cut(s) | 165, 216 |
| BseMI | GCAATG | 1 cut(s) | 261 |
| BseRI | GAGGAG | 1 cut(s) | 255 |
| BsmI | GAATGC | 1 cut(s) | 292 |
| BspACI | CCGC | 2 cut(s) | 39, 42 |
| BspOI | GCTAGC | 1 cut(s) | 234 |
| BsrBI | CCGCTC | 1 cut(s) | 44 |
| BsrDI | GCAATG | 1 cut(s) | 261 |
| BssNI | GRCGYC | 2 cut(s) | 58, 80 |
| BstACI | GRCGYC | 2 cut(s) | 58, 80 |
| BstC8I | GCNNGC | 3 cut(s) | 130, 181, 232 |
| BstF5I | GGATG | 2 cut(s) | 165, 216 |
| BtgZI | GCGATG | 2 cut(s) | 69, 291 |
| BtsCI | GGATG | 2 cut(s) | 165, 216 |
| Cac8I | GCNNGC | 3 cut(s) | 130, 181, 232 |
| CseI | GACGC | 2 cut(s) | 66, 88 |
| CviAII | CATG | 4 cut(s) | 133, 184, 235, 289 |
| CviJI | RGCY | 2 cut(s) | 52, 74 |
| CviKI_1 | RGCY | 2 cut(s) | 52, 74 |
| EcoT22I | ATGCAT | 1 cut(s) | 294 |
| FaeI | CATG | 4 cut(s) | 136, 187, 238, 292 |
| FaiI | YATR | 5 cut(s) | 9, 134, 185, 236, 290 |
| FatI | CATG | 4 cut(s) | 132, 183, 234, 288 |
| Fnu4HI | GCNGC | 2 cut(s) | 39, 42 |
| FokI | GGATG | 2 cut(s) | 172, 223 |
| Fsp4HI | GCNGC | 2 cut(s) | 39, 42 |
| FspBI | CTAG | 1 cut(s) | 231 |
| GluI | GCNGC | 2 cut(s) | 39, 42 |
| GsuI | CTGGAG | 2 cut(s) | 32, 54 |
| HgaI | GACGC | 2 cut(s) | 66, 88 |
| Hin1I | GRCGYC | 2 cut(s) | 58, 80 |
| Hin1II | CATG | 4 cut(s) | 136, 187, 238, 292 |
| HphI | GGTGA | 4 cut(s) | 152, 203, 257, 272 |
| Hpy188I | TCNGA | 2 cut(s) | 56, 78 |
| Hpy188III | TCNNGA | 1 cut(s) | 220 |
| Hpy99I | CGWCG | 3 cut(s) | 60, 66, 82 |
| HpyAV | CCTTC | 3 cut(s) | 37, 137, 188 |
| HpyCH4V | TGCA | 2 cut(s) | 254, 292 |
| Hsp92I | GRCGYC | 2 cut(s) | 58, 80 |
| Hsp92II | CATG | 4 cut(s) | 136, 187, 238, 292 |
| LmnI | GCTCC | 2 cut(s) | 57, 79 |
| LpnPI | CCDG | 4 cut(s) | 62, 84, 114, 165 |
| LweI | GCATC | 1 cut(s) | 241 |
| MaeI | CTAG | 1 cut(s) | 231 |
| MaeIII | GTNAC | 1 cut(s) | 260 |
| MbiI | CCGCTC | 1 cut(s) | 44 |
| MmeI | TCCRAC | 2 cut(s) | 79, 101 |
| MnlI | CCTC | 6 cut(s) | 109, 119, 131, 182, 233, 236 |
| Mph1103I | ATGCAT | 1 cut(s) | 294 |
| Mva1269I | GAATGC | 1 cut(s) | 292 |
| NheI | GCTAGC | 1 cut(s) | 230 |
| NlaIII | CATG | 4 cut(s) | 136, 187, 238, 292 |
| NmuCI | GTSAC | 1 cut(s) | 260 |
| NsiI | ATGCAT | 1 cut(s) | 294 |
| PctI | GAATGC | 1 cut(s) | 292 |
| PflFI | GACNNNGTC | 1 cut(s) | 60 |
| PkrI | GCNGC | 2 cut(s) | 40, 43 |
| PsyI | GACNNNGTC | 1 cut(s) | 60 |
| SatI | GCNGC | 2 cut(s) | 39, 42 |
| SetI | ASST | 8 cut(s) | 54, 76, 101, 142, 148, 193, 199, 247 |
| SfaNI | GCATC | 1 cut(s) | 241 |
| SsiI | CCGC | 2 cut(s) | 39, 42 |
| SspMI | CTAG | 1 cut(s) | 231 |
| TaqI | TCGA | 1 cut(s) | 221 |
| TauI | GCSGC | 2 cut(s) | 41, 44 |
| TseFI | GTSAC | 1 cut(s) | 260 |
| Tsp45I | GTSAC | 1 cut(s) | 260 |
| TspDTI | ATGAA | 2 cut(s) | 17, 277 |
| Tth111I | GACNNNGTC | 1 cut(s) | 60 |
| XspI | CTAG | 1 cut(s) | 231 |
| Zsp2I | ATGCAT | 1 cut(s) | 294 |