Rh7DG455200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0002163
Physical Location & Seq
Forward (+)
64741769 .. 64743139
1371 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG455200.1

Sequence Viewer

Length: 441 bp
ATGGTATATGGCCAAGTTCCACGTCTTCTCCTCTTCTCACAGTCCCAAAAAATTTTCAGAACCAAAATCCTTCCCTCCGATGAACCAGGTCAAGAATTTGACGACCCATGTCCAAAATTTGATATCAAACACGTCTTCTCTTCTCAATACCCAGAAGAATTGCACAACCCAGTTCTCATTCCGGATTCGTTCCCGCCGGAGACCCAAGATAATAATAGCCTTCGTGGTCGGAGCCGCTCTCCAGCTCCGACGCCGTCGCTTTCCACCACCTATCAGATCATTGTTTTGACTCTTAACTGTAATCTGCAGAAACTTGCTGGCATGGGAGGTGAAGGTGGAATGGGTGGGACGGATTTCTCGAAACTTGCTGGCATGGGAGGTGATGATGCAATGGGTGACTTTGATGATAGCGATGATGAGTTCATGCATTCTTGGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

146

Amino Acids

16.15

Weight (kDa)

4.5

Isoelectric Point (pI)

59.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 237
AccIII TCCGGA 1 cut(s) 181
AciI CCGC 2 cut(s) 194, 235
AcoI YGGCCR 1 cut(s) 10
AcsI RAATTY 3 cut(s) 51, 95, 116
AcyI GRCGYC 1 cut(s) 251
AflIII ACRYGT 1 cut(s) 130
AjiI CACGTC 2 cut(s) 23, 133
AjnI CCWGG 1 cut(s) 85
AluBI AGCT 1 cut(s) 245
AluI AGCT 1 cut(s) 245
Alw26I GTCTC 1 cut(s) 194
Aor13HI TCCGGA 1 cut(s) 181
AoxI GGCC 1 cut(s) 10
ApoI RAATTY 3 cut(s) 51, 95, 116
AsuHPI GGTGA 3 cut(s) 341, 392, 407
BalI TGGCCA 1 cut(s) 12
BbsI GAAGAC 2 cut(s) 17, 127
BceAI ACGGC 1 cut(s) 238
BciT130I CCWGG 1 cut(s) 87
BcoDI GTCTC 1 cut(s) 194
BfmI CTRYAG 1 cut(s) 305
BisI GCNGC 1 cut(s) 235
BlsI GCNGC 1 cut(s) 236
Bme1390I CCNGG 1 cut(s) 87
BmgBI CACGTC 2 cut(s) 23, 133
BmiI GGNNCC 1 cut(s) 233
BmrFI CCNGG 1 cut(s) 87
BmrI ACTGGG 1 cut(s) 164
BmsI GCATC 1 cut(s) 376
BmuI ACTGGG 1 cut(s) 164
BoxI GACNNNNGTC 1 cut(s) 108
BpiI GAAGAC 2 cut(s) 17, 127
BplI GAGNNNNNCTC 2 cut(s) 223, 255
BpmI CTGGAG 1 cut(s) 225
BsaHI GRCGYC 1 cut(s) 251
BsaI GGTCTC 1 cut(s) 194
BsaWI WCCGGW 1 cut(s) 181
BsaXI ACNNNNNCTCC 2 cut(s) 12, 42
Bse1I ACTGG 1 cut(s) 170
Bse3DI GCAATG 1 cut(s) 396
BseAI TCCGGA 1 cut(s) 181
BseBI CCWGG 1 cut(s) 87
BseMI GCAATG 1 cut(s) 396
BseNI ACTGG 1 cut(s) 170
BseRI GAGGAG 1 cut(s) 20
BshFI GGCC 1 cut(s) 12
BsiSI CCGG 2 cut(s) 182, 197
BslFI GGGAC 2 cut(s) 28, 361
BsmAI GTCTC 1 cut(s) 194
BsmFI GGGAC 2 cut(s) 28, 361
BsmI GAATGC 1 cut(s) 427
BsnI GGCC 1 cut(s) 12
Bso31I GGTCTC 1 cut(s) 194
Bsp13I TCCGGA 1 cut(s) 181
Bsp143I GATC 1 cut(s) 276
BspACI CCGC 2 cut(s) 194, 235
BspANI GGCC 1 cut(s) 12
BspEI TCCGGA 1 cut(s) 181
BspLI GGNNCC 1 cut(s) 233
BspMAI CTGCAG 1 cut(s) 309
BspTNI GGTCTC 1 cut(s) 194
BsrBI CCGCTC 1 cut(s) 237
BsrDI GCAATG 1 cut(s) 396
BsrI ACTGG 1 cut(s) 170
BssMI GATC 1 cut(s) 276
BssNI GRCGYC 1 cut(s) 251
Bst2UI CCWGG 1 cut(s) 87
Bst4CI ACNGT 2 cut(s) 42, 299
Bst6I CTCTTC 2 cut(s) 38, 145
BstACI GRCGYC 1 cut(s) 251
BstC8I GCNNGC 2 cut(s) 319, 370
BstKTI GATC 1 cut(s) 279
BstMAI GTCTC 1 cut(s) 194
BstMBI GATC 1 cut(s) 276
BstNI CCWGG 1 cut(s) 87
BstPAI GACNNNNGTC 1 cut(s) 108
BstSCI CCNGG 1 cut(s) 85
BstSFI CTRYAG 1 cut(s) 305
BstV2I GAAGAC 2 cut(s) 17, 127
BsuRI GGCC 1 cut(s) 12
BtgZI GCGATG 1 cut(s) 426
BtrI CACGTC 2 cut(s) 23, 133
Cac8I GCNNGC 2 cut(s) 319, 370
CseI GACGC 1 cut(s) 259
CsiI ACCWGGT 1 cut(s) 85
CviAII CATG 4 cut(s) 108, 322, 373, 424
CviJI RGCY 4 cut(s) 12, 219, 234, 245
CviKI_1 RGCY 4 cut(s) 12, 219, 234, 245
DpnI GATC 1 cut(s) 278
DpnII GATC 1 cut(s) 276
EaeI YGGCCR 1 cut(s) 10
Eam1104I CTCTTC 2 cut(s) 38, 145
EarI CTCTTC 2 cut(s) 38, 145
Eco31I GGTCTC 1 cut(s) 194
Eco32I GATATC 1 cut(s) 124
EcoRII CCWGG 1 cut(s) 85
EcoRV GATATC 1 cut(s) 124
EcoT22I ATGCAT 1 cut(s) 429
FaeI CATG 4 cut(s) 111, 325, 376, 427
FaiI YATR 6 cut(s) 7, 9, 109, 323, 374, 425
FaqI GGGAC 2 cut(s) 28, 361
FatI CATG 4 cut(s) 107, 321, 372, 423
FauI CCCGC 1 cut(s) 201
Fnu4HI GCNGC 1 cut(s) 235
Fsp4HI GCNGC 1 cut(s) 235
GluI GCNGC 1 cut(s) 235
GsuI CTGGAG 1 cut(s) 225
HaeIII GGCC 1 cut(s) 12
HapII CCGG 2 cut(s) 182, 197
HgaI GACGC 1 cut(s) 259
Hin1I GRCGYC 1 cut(s) 251
Hin1II CATG 4 cut(s) 111, 325, 376, 427
HinfI GANTC 2 cut(s) 185, 289
HpaII CCGG 2 cut(s) 182, 197
HphI GGTGA 3 cut(s) 341, 392, 407
Hpy188I TCNGA 5 cut(s) 59, 79, 231, 249, 276
Hpy188III TCNNGA 3 cut(s) 92, 182, 358
Hpy99I CGWCG 2 cut(s) 253, 259
HpyAV CCTTC 3 cut(s) 80, 230, 326
HpyCH4III ACNGT 2 cut(s) 42, 299
HpyCH4IV ACGT 2 cut(s) 22, 132
HpyCH4V TGCA 4 cut(s) 163, 307, 389, 427
HpySE526I ACGT 2 cut(s) 22, 132
Hsp92I GRCGYC 1 cut(s) 251
Hsp92II CATG 4 cut(s) 111, 325, 376, 427
Kpn2I TCCGGA 1 cut(s) 181
Kzo9I GATC 1 cut(s) 276
LmnI GCTCC 2 cut(s) 231, 250
LpnPI CCDG 9 cut(s) 72, 99, 165, 183, 195, 210, 255, 303, 354
LweI GCATC 1 cut(s) 376
MabI ACCWGGT 1 cut(s) 85
MaeII ACGT 2 cut(s) 22, 132
MaeIII GTNAC 1 cut(s) 395
MalI GATC 1 cut(s) 278
MbiI CCGCTC 1 cut(s) 237
MboI GATC 1 cut(s) 276
MboII GAAGA 5 cut(s) 17, 25, 127, 132, 167
MlsI TGGCCA 1 cut(s) 12
MluCI AATT 4 cut(s) 51, 95, 116, 158
MluNI TGGCCA 1 cut(s) 12
MlyI GAGTC 1 cut(s) 283
MmeI TCCRAC 2 cut(s) 209, 272
MnlI CCTC 4 cut(s) 41, 85, 320, 371
Mox20I TGGCCA 1 cut(s) 12
Mph1103I ATGCAT 1 cut(s) 429
MroI TCCGGA 1 cut(s) 181
MscI TGGCCA 1 cut(s) 12
MseI TTAA 1 cut(s) 294
Msp20I TGGCCA 1 cut(s) 12
MspI CCGG 2 cut(s) 182, 197
MspR9I CCNGG 1 cut(s) 87
Mva1269I GAATGC 1 cut(s) 427
MvaI CCWGG 1 cut(s) 87
NdeII GATC 1 cut(s) 276
NlaIII CATG 4 cut(s) 111, 325, 376, 427
NlaIV GGNNCC 1 cut(s) 233
NmuCI GTSAC 1 cut(s) 395
NsiI ATGCAT 1 cut(s) 429
PcsI WCGNNNNNNNCGW 1 cut(s) 356
PctI GAATGC 1 cut(s) 427
PfeI GAWTC 1 cut(s) 185
PflFI GACNNNGTC 1 cut(s) 253
PkrI GCNGC 1 cut(s) 236
PleI GAGTC 1 cut(s) 283
PpsI GAGTC 1 cut(s) 283
PshAI GACNNNNGTC 1 cut(s) 108
Psp6I CCWGG 1 cut(s) 85
PspGI CCWGG 1 cut(s) 85
PspN4I GGNNCC 1 cut(s) 233
PstI CTGCAG 1 cut(s) 309
PsyI GACNNNGTC 1 cut(s) 253
SaqAI TTAA 1 cut(s) 294
SatI GCNGC 1 cut(s) 235
Sau3AI GATC 1 cut(s) 276
SchI GAGTC 1 cut(s) 283
ScrFI CCNGG 1 cut(s) 87
SetI ASST 8 cut(s) 25, 91, 135, 247, 272, 331, 337, 382
SexAI ACCWGGT 1 cut(s) 85
SfaNI GCATC 1 cut(s) 376
SfcI CTRYAG 1 cut(s) 305
Sse9I AATT 4 cut(s) 51, 95, 116, 158
SsiI CCGC 2 cut(s) 194, 235
StyD4I CCNGG 1 cut(s) 85
TaaI ACNGT 2 cut(s) 42, 299
TaiI ACGT 2 cut(s) 25, 135
TaqI TCGA 1 cut(s) 359
TasI AATT 4 cut(s) 51, 95, 116, 158
TauI GCSGC 1 cut(s) 237
TfiI GAWTC 1 cut(s) 185
Tru1I TTAA 1 cut(s) 294
Tru9I TTAA 1 cut(s) 294
TseFI GTSAC 1 cut(s) 395
Tsp45I GTSAC 1 cut(s) 395
TspDTI ATGAA 2 cut(s) 96, 412
TspGWI ACGGA 1 cut(s) 365
Tth111I GACNNNGTC 1 cut(s) 253
XapI RAATTY 3 cut(s) 51, 95, 116
Zsp2I ATGCAT 1 cut(s) 429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.