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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 1 cut(s) | 2 |
| AgsI | TTSAA | 3 cut(s) | 57, 169, 227 |
| AluBI | AGCT | 1 cut(s) | 183 |
| AluI | AGCT | 1 cut(s) | 183 |
| AlwI | GGATC | 1 cut(s) | 2 |
| AoxI | GGCC | 1 cut(s) | 12 |
| AspS9I | GGNCC | 1 cut(s) | 90 |
| AvaII | GGWCC | 1 cut(s) | 90 |
| BfmI | CTRYAG | 1 cut(s) | 98 |
| Bme18I | GGWCC | 1 cut(s) | 90 |
| BmgT120I | GGNCC | 1 cut(s) | 90 |
| Bse1I | ACTGG | 1 cut(s) | 36 |
| BseNI | ACTGG | 1 cut(s) | 36 |
| Bsh1285I | CGRYCG | 1 cut(s) | 126 |
| BshFI | GGCC | 1 cut(s) | 14 |
| BsiEI | CGRYCG | 1 cut(s) | 126 |
| BsiSI | CCGG | 1 cut(s) | 11 |
| BsnI | GGCC | 1 cut(s) | 14 |
| Bsp143I | GATC | 3 cut(s) | 7, 123, 150 |
| BspANI | GGCC | 1 cut(s) | 14 |
| BspHI | TCATGA | 1 cut(s) | 219 |
| BspMAI | CTGCAG | 1 cut(s) | 102 |
| BspPI | GGATC | 1 cut(s) | 2 |
| BsrI | ACTGG | 1 cut(s) | 36 |
| BssMI | GATC | 3 cut(s) | 7, 123, 150 |
| BstC8I | GCNNGC | 1 cut(s) | 181 |
| BstDEI | CTNAG | 2 cut(s) | 86, 147 |
| BstKTI | GATC | 3 cut(s) | 10, 126, 153 |
| BstMBI | GATC | 3 cut(s) | 7, 123, 150 |
| BstMCI | CGRYCG | 1 cut(s) | 126 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 11 |
| BstSFI | CTRYAG | 1 cut(s) | 98 |
| BstX2I | RGATCY | 1 cut(s) | 7 |
| BstYI | RGATCY | 1 cut(s) | 7 |
| BsuRI | GGCC | 1 cut(s) | 14 |
| BtsIMutI | CAGTG | 1 cut(s) | 29 |
| Cac8I | GCNNGC | 1 cut(s) | 181 |
| CciI | TCATGA | 1 cut(s) | 219 |
| Cfr13I | GGNCC | 1 cut(s) | 90 |
| CviAII | CATG | 2 cut(s) | 194, 220 |
| CviJI | RGCY | 3 cut(s) | 14, 159, 183 |
| CviKI_1 | RGCY | 3 cut(s) | 14, 159, 183 |
| DdeI | CTNAG | 2 cut(s) | 86, 147 |
| DpnI | GATC | 3 cut(s) | 9, 125, 152 |
| DpnII | GATC | 3 cut(s) | 7, 123, 150 |
| Eco47I | GGWCC | 1 cut(s) | 90 |
| EcoO109I | RGGNCCY | 1 cut(s) | 90 |
| FaeI | CATG | 2 cut(s) | 197, 223 |
| FaiI | YATR | 4 cut(s) | 72, 141, 195, 221 |
| FatI | CATG | 2 cut(s) | 193, 219 |
| HaeIII | GGCC | 1 cut(s) | 14 |
| HapII | CCGG | 1 cut(s) | 11 |
| Hin1II | CATG | 2 cut(s) | 197, 223 |
| HindIII | AAGCTT | 1 cut(s) | 181 |
| HpaII | CCGG | 1 cut(s) | 11 |
| Hpy188III | TCNNGA | 1 cut(s) | 220 |
| HpyCH4V | TGCA | 2 cut(s) | 100, 193 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 11 |
| HpyF3I | CTNAG | 2 cut(s) | 86, 147 |
| Hsp92II | CATG | 2 cut(s) | 197, 223 |
| Kzo9I | GATC | 3 cut(s) | 7, 123, 150 |
| LmnI | GCTCC | 1 cut(s) | 45 |
| LpnPI | CCDG | 4 cut(s) | 17, 24, 51, 86 |
| MalI | GATC | 3 cut(s) | 9, 125, 152 |
| MboI | GATC | 3 cut(s) | 7, 123, 150 |
| MflI | RGATCY | 1 cut(s) | 7 |
| MseI | TTAA | 1 cut(s) | 77 |
| MspI | CCGG | 1 cut(s) | 11 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 11 |
| NdeII | GATC | 3 cut(s) | 7, 123, 150 |
| NlaIII | CATG | 2 cut(s) | 197, 223 |
| PagI | TCATGA | 1 cut(s) | 219 |
| Ple19I | CGATCG | 1 cut(s) | 126 |
| PpuMI | RGGWCCY | 1 cut(s) | 90 |
| Psp5II | RGGWCCY | 1 cut(s) | 90 |
| PspPI | GGNCC | 1 cut(s) | 90 |
| PspPPI | RGGWCCY | 1 cut(s) | 90 |
| PstI | CTGCAG | 1 cut(s) | 102 |
| PsuI | RGATCY | 1 cut(s) | 7 |
| PvuI | CGATCG | 1 cut(s) | 126 |
| SaqAI | TTAA | 1 cut(s) | 77 |
| Sau3AI | GATC | 3 cut(s) | 7, 123, 150 |
| Sau96I | GGNCC | 1 cut(s) | 90 |
| SetI | ASST | 2 cut(s) | 92, 185 |
| SfcI | CTRYAG | 1 cut(s) | 98 |
| SinI | GGWCC | 1 cut(s) | 90 |
| TaqI | TCGA | 1 cut(s) | 122 |
| Tru1I | TTAA | 1 cut(s) | 77 |
| Tru9I | TTAA | 1 cut(s) | 77 |
| TscAI | CASTG | 1 cut(s) | 36 |
| TspRI | CASTG | 1 cut(s) | 36 |
| VpaK11BI | GGWCC | 1 cut(s) | 90 |