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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 188 |
| AccB1I | GGYRCC | 1 cut(s) | 188 |
| AccIII | TCCGGA | 1 cut(s) | 102 |
| AciI | CCGC | 1 cut(s) | 115 |
| AcsI | RAATTY | 3 cut(s) | 16, 37, 208 |
| AfaI | GTAC | 1 cut(s) | 190 |
| AflIII | ACRYGT | 1 cut(s) | 51 |
| AgsI | TTSAA | 3 cut(s) | 208, 216, 315 |
| AjiI | CACGTC | 1 cut(s) | 54 |
| AluBI | AGCT | 2 cut(s) | 272, 444 |
| AluI | AGCT | 2 cut(s) | 272, 444 |
| Alw26I | GTCTC | 2 cut(s) | 115, 205 |
| Aor13HI | TCCGGA | 1 cut(s) | 102 |
| ApoI | RAATTY | 3 cut(s) | 16, 37, 208 |
| Asp718I | GGTACC | 1 cut(s) | 188 |
| AspS9I | GGNCC | 1 cut(s) | 145 |
| AsuHPI | GGTGA | 4 cut(s) | 296, 347, 401, 416 |
| AvaII | GGWCC | 1 cut(s) | 145 |
| BanI | GGYRCC | 1 cut(s) | 188 |
| BbsI | GAAGAC | 2 cut(s) | 48, 440 |
| BccI | CCATC | 3 cut(s) | 298, 349, 419 |
| BcoDI | GTCTC | 2 cut(s) | 115, 205 |
| BfaI | CTAG | 1 cut(s) | 269 |
| BfmI | CTRYAG | 1 cut(s) | 260 |
| Bme18I | GGWCC | 1 cut(s) | 145 |
| BmgBI | CACGTC | 1 cut(s) | 54 |
| BmgT120I | GGNCC | 1 cut(s) | 145 |
| BmiI | GGNNCC | 2 cut(s) | 147, 190 |
| BmrI | ACTGGG | 1 cut(s) | 85 |
| BmsI | GCATC | 1 cut(s) | 385 |
| BmuI | ACTGGG | 1 cut(s) | 85 |
| BpiI | GAAGAC | 2 cut(s) | 48, 440 |
| BsaI | GGTCTC | 1 cut(s) | 115 |
| BsaJI | CCNNGG | 1 cut(s) | 141 |
| BsaWI | WCCGGW | 1 cut(s) | 102 |
| Bse1I | ACTGG | 1 cut(s) | 91 |
| Bse3DI | GCAATG | 1 cut(s) | 405 |
| BseAI | TCCGGA | 1 cut(s) | 102 |
| BseDI | CCNNGG | 1 cut(s) | 141 |
| BseGI | GGATG | 2 cut(s) | 309, 360 |
| BseMI | GCAATG | 1 cut(s) | 405 |
| BseNI | ACTGG | 1 cut(s) | 91 |
| BseRI | GAGGAG | 1 cut(s) | 399 |
| BshNI | GGYRCC | 1 cut(s) | 188 |
| BsiSI | CCGG | 1 cut(s) | 103 |
| BslFI | GGGAC | 1 cut(s) | 131 |
| BsmAI | GTCTC | 2 cut(s) | 115, 205 |
| BsmFI | GGGAC | 1 cut(s) | 131 |
| Bso31I | GGTCTC | 1 cut(s) | 115 |
| Bsp13I | TCCGGA | 1 cut(s) | 102 |
| BspACI | CCGC | 1 cut(s) | 115 |
| BspEI | TCCGGA | 1 cut(s) | 102 |
| BspLI | GGNNCC | 2 cut(s) | 147, 190 |
| BspMAI | CTGCAG | 1 cut(s) | 264 |
| BspT107I | GGYRCC | 1 cut(s) | 188 |
| BspTNI | GGTCTC | 1 cut(s) | 115 |
| BsrDI | GCAATG | 1 cut(s) | 405 |
| BsrI | ACTGG | 1 cut(s) | 91 |
| BssECI | CCNNGG | 1 cut(s) | 141 |
| Bst6I | CTCTTC | 1 cut(s) | 66 |
| BstC8I | GCNNGC | 3 cut(s) | 274, 325, 376 |
| BstDSI | CCRYGG | 1 cut(s) | 141 |
| BstF5I | GGATG | 2 cut(s) | 309, 360 |
| BstMAI | GTCTC | 2 cut(s) | 115, 205 |
| BstSFI | CTRYAG | 1 cut(s) | 260 |
| BstV2I | GAAGAC | 2 cut(s) | 48, 440 |
| BtgI | CCRYGG | 1 cut(s) | 141 |
| BtgZI | GCGATG | 1 cut(s) | 435 |
| BtrI | CACGTC | 1 cut(s) | 54 |
| BtsCI | GGATG | 2 cut(s) | 309, 360 |
| Cac8I | GCNNGC | 3 cut(s) | 274, 325, 376 |
| Cfr13I | GGNCC | 1 cut(s) | 145 |
| Csp6I | GTAC | 1 cut(s) | 189 |
| CviAII | CATG | 5 cut(s) | 8, 29, 277, 328, 379 |
| CviJI | RGCY | 3 cut(s) | 137, 272, 444 |
| CviKI_1 | RGCY | 3 cut(s) | 137, 272, 444 |
| CviQI | GTAC | 1 cut(s) | 189 |
| Eam1104I | CTCTTC | 1 cut(s) | 66 |
| EarI | CTCTTC | 1 cut(s) | 66 |
| Eco31I | GGTCTC | 1 cut(s) | 115 |
| Eco32I | GATATC | 1 cut(s) | 45 |
| Eco47I | GGWCC | 1 cut(s) | 145 |
| EcoRV | GATATC | 1 cut(s) | 45 |
| FaeI | CATG | 5 cut(s) | 11, 32, 280, 331, 382 |
| FaiI | YATR | 5 cut(s) | 9, 30, 278, 329, 380 |
| FaqI | GGGAC | 1 cut(s) | 131 |
| FatI | CATG | 5 cut(s) | 7, 28, 276, 327, 378 |
| FauI | CCCGC | 1 cut(s) | 122 |
| FokI | GGATG | 2 cut(s) | 316, 367 |
| FspBI | CTAG | 1 cut(s) | 269 |
| HapII | CCGG | 1 cut(s) | 103 |
| Hin1II | CATG | 5 cut(s) | 11, 32, 280, 331, 382 |
| HinfI | GANTC | 1 cut(s) | 106 |
| HpaII | CCGG | 1 cut(s) | 103 |
| HphI | GGTGA | 4 cut(s) | 296, 347, 401, 416 |
| Hpy188III | TCNNGA | 4 cut(s) | 13, 34, 103, 364 |
| Hpy99I | CGWCG | 1 cut(s) | 155 |
| HpyAV | CCTTC | 3 cut(s) | 202, 281, 332 |
| HpyCH4IV | ACGT | 2 cut(s) | 53, 251 |
| HpyCH4V | TGCA | 3 cut(s) | 84, 262, 398 |
| HpySE526I | ACGT | 2 cut(s) | 53, 251 |
| Hsp92II | CATG | 5 cut(s) | 11, 32, 280, 331, 382 |
| Kpn2I | TCCGGA | 1 cut(s) | 102 |
| KpnI | GGTACC | 1 cut(s) | 192 |
| LpnPI | CCDG | 7 cut(s) | 86, 104, 116, 131, 258, 309, 360 |
| LweI | GCATC | 1 cut(s) | 385 |
| MaeI | CTAG | 1 cut(s) | 269 |
| MaeII | ACGT | 2 cut(s) | 53, 251 |
| MaeIII | GTNAC | 1 cut(s) | 404 |
| MboII | GAAGA | 5 cut(s) | 48, 53, 88, 237, 445 |
| MfeI | CAATTG | 1 cut(s) | 216 |
| MluCI | AATT | 5 cut(s) | 16, 37, 79, 208, 216 |
| MnlI | CCTC | 7 cut(s) | 148, 176, 186, 275, 326, 377, 380 |
| MroI | TCCGGA | 1 cut(s) | 102 |
| MseI | TTAA | 1 cut(s) | 446 |
| MspI | CCGG | 1 cut(s) | 103 |
| MunI | CAATTG | 1 cut(s) | 216 |
| NlaIII | CATG | 5 cut(s) | 11, 32, 280, 331, 382 |
| NlaIV | GGNNCC | 2 cut(s) | 147, 190 |
| NmuCI | GTSAC | 1 cut(s) | 404 |
| PfeI | GAWTC | 1 cut(s) | 106 |
| PspN4I | GGNNCC | 2 cut(s) | 147, 190 |
| PspPI | GGNCC | 1 cut(s) | 145 |
| PstI | CTGCAG | 1 cut(s) | 264 |
| RsaI | GTAC | 1 cut(s) | 190 |
| RsaNI | GTAC | 1 cut(s) | 189 |
| SaqAI | TTAA | 1 cut(s) | 446 |
| Sau96I | GGNCC | 1 cut(s) | 145 |
| SfaNI | GCATC | 1 cut(s) | 385 |
| SfcI | CTRYAG | 1 cut(s) | 260 |
| SinI | GGWCC | 1 cut(s) | 145 |
| Sse9I | AATT | 5 cut(s) | 16, 37, 79, 208, 216 |
| SsiI | CCGC | 1 cut(s) | 115 |
| SspMI | CTAG | 1 cut(s) | 269 |
| TaiI | ACGT | 2 cut(s) | 56, 254 |
| TaqI | TCGA | 1 cut(s) | 365 |
| TasI | AATT | 5 cut(s) | 16, 37, 79, 208, 216 |
| TfiI | GAWTC | 1 cut(s) | 106 |
| Tru1I | TTAA | 1 cut(s) | 446 |
| Tru9I | TTAA | 1 cut(s) | 446 |
| TseFI | GTSAC | 1 cut(s) | 404 |
| Tsp45I | GTSAC | 1 cut(s) | 404 |
| TspDTI | ATGAA | 1 cut(s) | 17 |
| TspGWI | ACGGA | 1 cut(s) | 130 |
| VpaK11BI | GGWCC | 1 cut(s) | 145 |
| XapI | RAATTY | 3 cut(s) | 16, 37, 208 |
| XspI | CTAG | 1 cut(s) | 269 |