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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 2 cut(s) | 95, 123 |
| AcsI | RAATTY | 1 cut(s) | 11 |
| AcyI | GRCGYC | 1 cut(s) | 149 |
| AgsI | TTSAA | 1 cut(s) | 237 |
| AluBI | AGCT | 2 cut(s) | 143, 366 |
| AluI | AGCT | 2 cut(s) | 143, 366 |
| Alw26I | GTCTC | 1 cut(s) | 95 |
| ApoI | RAATTY | 1 cut(s) | 11 |
| AspS9I | GGNCC | 1 cut(s) | 125 |
| AsuHPI | GGTGA | 4 cut(s) | 218, 269, 323, 338 |
| AvaII | GGWCC | 1 cut(s) | 125 |
| BbsI | GAAGAC | 1 cut(s) | 362 |
| BccI | CCATC | 3 cut(s) | 220, 271, 341 |
| BceAI | ACGGC | 1 cut(s) | 136 |
| BcoDI | GTCTC | 1 cut(s) | 95 |
| Bme18I | GGWCC | 1 cut(s) | 125 |
| BmgT120I | GGNCC | 1 cut(s) | 125 |
| BmiI | GGNNCC | 1 cut(s) | 127 |
| BmrI | ACTGGG | 1 cut(s) | 65 |
| BmsI | GCATC | 1 cut(s) | 307 |
| BmuI | ACTGGG | 1 cut(s) | 65 |
| BpiI | GAAGAC | 1 cut(s) | 362 |
| BpmI | CTGGAG | 1 cut(s) | 123 |
| BsaHI | GRCGYC | 1 cut(s) | 149 |
| BsaI | GGTCTC | 1 cut(s) | 95 |
| Bse1I | ACTGG | 1 cut(s) | 71 |
| Bse3DI | GCAATG | 1 cut(s) | 327 |
| BseGI | GGATG | 2 cut(s) | 231, 282 |
| BseMI | GCAATG | 1 cut(s) | 327 |
| BseNI | ACTGG | 1 cut(s) | 71 |
| BseRI | GAGGAG | 1 cut(s) | 321 |
| BslFI | GGGAC | 1 cut(s) | 111 |
| BsmAI | GTCTC | 1 cut(s) | 95 |
| BsmFI | GGGAC | 1 cut(s) | 111 |
| Bso31I | GGTCTC | 1 cut(s) | 95 |
| BspACI | CCGC | 2 cut(s) | 95, 123 |
| BspLI | GGNNCC | 1 cut(s) | 127 |
| BspTNI | GGTCTC | 1 cut(s) | 95 |
| BsrDI | GCAATG | 1 cut(s) | 327 |
| BsrI | ACTGG | 1 cut(s) | 71 |
| BssNI | GRCGYC | 1 cut(s) | 149 |
| BstACI | GRCGYC | 1 cut(s) | 149 |
| BstC8I | GCNNGC | 3 cut(s) | 196, 247, 298 |
| BstF5I | GGATG | 2 cut(s) | 231, 282 |
| BstMAI | GTCTC | 1 cut(s) | 95 |
| BstV2I | GAAGAC | 1 cut(s) | 362 |
| BtgZI | GCGATG | 1 cut(s) | 357 |
| BtsCI | GGATG | 2 cut(s) | 231, 282 |
| Cac8I | GCNNGC | 3 cut(s) | 196, 247, 298 |
| Cfr13I | GGNCC | 1 cut(s) | 125 |
| CseI | GACGC | 1 cut(s) | 157 |
| CviAII | CATG | 3 cut(s) | 199, 250, 301 |
| CviJI | RGCY | 4 cut(s) | 48, 117, 143, 366 |
| CviKI_1 | RGCY | 4 cut(s) | 48, 117, 143, 366 |
| Eco31I | GGTCTC | 1 cut(s) | 95 |
| Eco47I | GGWCC | 1 cut(s) | 125 |
| FaeI | CATG | 3 cut(s) | 202, 253, 304 |
| FaiI | YATR | 3 cut(s) | 200, 251, 302 |
| FaqI | GGGAC | 1 cut(s) | 111 |
| FatI | CATG | 3 cut(s) | 198, 249, 300 |
| FauI | CCCGC | 1 cut(s) | 102 |
| FokI | GGATG | 2 cut(s) | 238, 289 |
| GsuI | CTGGAG | 1 cut(s) | 123 |
| HgaI | GACGC | 1 cut(s) | 157 |
| Hin1I | GRCGYC | 1 cut(s) | 149 |
| Hin1II | CATG | 3 cut(s) | 202, 253, 304 |
| HinfI | GANTC | 1 cut(s) | 86 |
| HphI | GGTGA | 4 cut(s) | 218, 269, 323, 338 |
| Hpy188I | TCNGA | 1 cut(s) | 147 |
| Hpy188III | TCNNGA | 3 cut(s) | 83, 180, 286 |
| Hpy99I | CGWCG | 2 cut(s) | 135, 157 |
| HpyAV | CCTTC | 3 cut(s) | 39, 203, 254 |
| HpyCH4V | TGCA | 3 cut(s) | 19, 64, 320 |
| Hsp92I | GRCGYC | 1 cut(s) | 149 |
| Hsp92II | CATG | 3 cut(s) | 202, 253, 304 |
| LpnPI | CCDG | 8 cut(s) | 68, 84, 111, 153, 165, 180, 231, 282 |
| LweI | GCATC | 1 cut(s) | 307 |
| MaeIII | GTNAC | 1 cut(s) | 326 |
| MboII | GAAGA | 2 cut(s) | 68, 367 |
| MluCI | AATT | 2 cut(s) | 11, 59 |
| MnlI | CCTC | 7 cut(s) | 36, 128, 185, 197, 248, 299, 302 |
| MseI | TTAA | 2 cut(s) | 50, 368 |
| NlaIII | CATG | 3 cut(s) | 202, 253, 304 |
| NlaIV | GGNNCC | 1 cut(s) | 127 |
| NmuCI | GTSAC | 1 cut(s) | 326 |
| PfeI | GAWTC | 1 cut(s) | 86 |
| PflFI | GACNNNGTC | 1 cut(s) | 151 |
| PspN4I | GGNNCC | 1 cut(s) | 127 |
| PspPI | GGNCC | 1 cut(s) | 125 |
| PsyI | GACNNNGTC | 1 cut(s) | 151 |
| SaqAI | TTAA | 2 cut(s) | 50, 368 |
| Sau96I | GGNCC | 1 cut(s) | 125 |
| SetI | ASST | 8 cut(s) | 145, 170, 208, 214, 259, 265, 313, 368 |
| SfaNI | GCATC | 1 cut(s) | 307 |
| SinI | GGWCC | 1 cut(s) | 125 |
| Sse9I | AATT | 2 cut(s) | 11, 59 |
| SsiI | CCGC | 2 cut(s) | 95, 123 |
| TaqI | TCGA | 1 cut(s) | 287 |
| TasI | AATT | 2 cut(s) | 11, 59 |
| TfiI | GAWTC | 1 cut(s) | 86 |
| Tru1I | TTAA | 2 cut(s) | 50, 368 |
| Tru9I | TTAA | 2 cut(s) | 50, 368 |
| TseFI | GTSAC | 1 cut(s) | 326 |
| Tsp45I | GTSAC | 1 cut(s) | 326 |
| TspDTI | ATGAA | 1 cut(s) | 24 |
| Tth111I | GACNNNGTC | 1 cut(s) | 151 |
| VpaK11BI | GGWCC | 1 cut(s) | 125 |
| XapI | RAATTY | 1 cut(s) | 11 |