Rmu_sc0001750.1_g000005

Domain of unknown function (DUF4283)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001750.1
OG0002163
Physical Location & Seq
Forward (+)
19079 .. 26939
7861 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001750.1_g000005.1.cds

Sequence Viewer

Length: 2487 bp
atgccgcagaagagacctgtcaggggccttcgagcttcagaatggagcccagtcgtcgatgacgaggttgatgtcggggatgcgagcaacgaggccgtcggagaagctgtggccctggtgttccggcaaagtcagttttggagaagtggtctccccaccggatctccgcctcctaatcgaacgccatttcccgctcccataaaatctcaaacaatgtcgttcagcttctcgcctcaatctccggcagcctgtcgaggccgagatcggccagccggtgataggaattcggaggccggtgatcggattcgggcgaaagttggccggattccggcgaccggtgatcggattcgggcgaaagttggcccgattccggtgaccggtgaccggactccggcgaaatgccctatggaaagcgaaaatctgcgtacgcgggtttccgcccttgcgtcgccgatcgccatggcggcttctacctctactgagccaagggcaatctgtccttggccttttctgaatctgccgtcggccacggagaagccttcgccacctttggcacaggccaccacaccgaagactttgcttcagttctttctgggtctgttgagtccacggtggctcttagctaactacctactccagtcattcgtgcctgatttcaagcctggggacactctccctcagacccattcccaaatctgggttaggttttatggactgagtcaagattactggcacccgcagcatctaatagagattgcttgtggagtaggcacacctttgcaattggacagggcaactaaagaaagagaatttggctactttgctagggttcttgtggatgttgatctagcaggtgattttccatctacactgatggttgaaagagaatctcattgttttccaattgaggttatttatgaaaatatgtgtgagaattgtgggattgttggacacacaatggatcgatgtaaacatcttattaaagagcaggctgaggcttctacttattccaagggggttgttggtagtgaacgcccaatgcccaaggctgtgaaccaggaacttaaccgtattgctgatgtggttgtcaatgaaaccattgtcaaccatgtggatatacctgcagaacctgtgttacaacagctttcctcctggagaatggagcaggggtctcattcatcatcatcctcttcagatgatgaacgtactatatctgacacattgggttctcatgtcagcaataccattattaatcaatcaacttcattggtgcgcaggccttggcactgtctcgttgaggaggaacaagagggtgatttgagtgattcaagtgctactcagctacaggggcctcttattcgtttgtcaaatcatgttaatgctgttttagagcaagggaatgttagtgaagcttcctttaatctagctcttgaagcccaggaggtggtaacttcaaaagtgttaacgaagtcccaaagaaaacgtcttgttaatagccccacgagacgaacactccgtagtttggttaagaagcattctccagattttctttgtcttgcagaaccaatggctgccatttcgaaaatcaagcaaactttctggcagtctataggtttctttgggactctactagtaatgggtgattttaatgctgttcttggagctcatgaaaaatttgggggaaacttaccttgtagagtgtcttgcgatgaatttcaagcaatggtcgacacttgtgggttggtgcaaattgatatcaagggttctcctttgacttggacaaatggtagaggggatcaattctggtctaccaaggcacatgaaaaatgggtgcgagatggtgacagaaatacgggttattatcaaaggattgctaaggttcgatgtgctcggtcttgtctttctcgcttacgcatcggttctgtgcttcatgatgatgtaaatattttgcgcgagcatgtggtcgatcacttcattaaagcatttacaaatgatgggcgctcagcggatacaggtttagtttcgaaactcattctgtgttttgttaccctggaggaaaatggtagccttacagcaattccatcctttgaggaggttgagctaatttttttttctatggatccggatagtgtacctggtcctgatggttgtaatggtcatttttaccgtgcttgttggagttttattggtcctgatgtggccacctcaggccggcgcacggacaccatcgccttcgtctcgtcgtcgccgtcacgactgtggtcttcgatcgcccatgcaccggacgaggagggagaaacgaccgtgagaagatctgactgctccggcgaagttcagccatttccggccaccttctgggccacattcgttcaggcgttgttcttctacgcctgcgatttctctggcttttcttggttcgttcaggcttcgctgctcctgttgatgaaggctaacaacgatttggaactgggtttaggaactcgctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 zf-CCHC_4

Protein Analysis

828

Amino Acids

91.48

Weight (kDa)

6.07

Isoelectric Point (pI)

49.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 842
Acc16I TGCGCA 1 cut(s) 1274
Acc36I ACCTGC 2 cut(s) 842, 1129
AccB1I GGYRCC 1 cut(s) 732
AccB7I CCANNNNNTGG 2 cut(s) 1444, 2355
AccBSI CCGCTC 1 cut(s) 194
AccI GTMKAC 2 cut(s) 1728, 1808
AccII CGCG 2 cut(s) 430, 1953
AccIII TCCGGA 1 cut(s) 2122
AciI CCGC 8 cut(s) 5, 167, 192, 430, 438, 464, 737, 2006
AclWI GGATC 5 cut(s) 169, 969, 1803, 2114, 2127
AcoI YGGCCR 5 cut(s) 266, 319, 525, 2199, 2346
AcsI RAATTY 4 cut(s) 283, 809, 1673, 1712
AcuI CTGAAG 3 cut(s) 21, 566, 1176
AfaI GTAC 3 cut(s) 427, 1207, 2133
AgeI ACCGGT 2 cut(s) 335, 377
AgsI TTSAA 6 cut(s) 658, 881, 1329, 1433, 1455, 1718
AhlI ACTAGT 1 cut(s) 1630
AjnI CCWGG 7 cut(s) 114, 661, 1056, 1151, 1437, 2049, 2134
AjuI GAANNNNNNNTTGG 2 cut(s) 795, 827
AleI CACNNNNGTG 1 cut(s) 2257
AloI GAACNNNNNNTCC 2 cut(s) 172, 204
Alw21I GWGCWC 2 cut(s) 1666, 1891
Alw26I GTCTC 6 cut(s) 7, 155, 1176, 1295, 1498, 2242
AlwI GGATC 5 cut(s) 169, 969, 1803, 2114, 2127
AlwNI CAGNNNCTG 1 cut(s) 1130
Aor13HI TCCGGA 1 cut(s) 2122
ApeKI GCWGC 4 cut(s) 245, 739, 1571, 2431
ApoI RAATTY 4 cut(s) 283, 809, 1673, 1712
ArsI GACNNNNNNTTYG 2 cut(s) 1468, 1500
AseI ATTAAT 1 cut(s) 1251
AsiGI ACCGGT 2 cut(s) 335, 377
AspLEI GCGC 4 cut(s) 1275, 1953, 2001, 2217
AspS9I GGNCC 7 cut(s) 25, 112, 362, 1349, 2138, 2189, 2358
AsuHPI GGTGA 9 cut(s) 287, 308, 350, 385, 392, 866, 1325, 1652, 1853
AsuII TTCGAA 2 cut(s) 1580, 2024
AvaII GGWCC 2 cut(s) 2138, 2189
AxyI CCTNAGG 1 cut(s) 2206
BalI TGGCCA 1 cut(s) 2201
BamHI GGATCC 1 cut(s) 2119
BanI GGYRCC 1 cut(s) 732
BanII GRGCYC 2 cut(s) 50, 1666
BauI CACGAG 1 cut(s) 1501
BbsI GAAGAC 2 cut(s) 578, 2256
Bbv12I GWGCWC 2 cut(s) 1666, 1891
BbvCI CCTCAGC 1 cut(s) 993
BbvI GCAGC 4 cut(s) 257, 751, 1558, 2418
BccI CCATC 7 cut(s) 868, 871, 1832, 1988, 2089, 2138, 2234
BceAI ACGGC 3 cut(s) 80, 505, 2233
BcgI CGANNNNNNTGC 2 cut(s) 559, 593
BciT130I CCWGG 7 cut(s) 116, 663, 1058, 1153, 1439, 2051, 2136
BciVI GTATCC 1 cut(s) 2002
BcoDI GTCTC 6 cut(s) 7, 155, 1176, 1295, 1498, 2242
BcuI ACTAGT 1 cut(s) 1630
BfaI CTAG 4 cut(s) 825, 848, 1424, 1631
BfmI CTRYAG 3 cut(s) 1122, 1343, 1608
BfoI RGCGCY 1 cut(s) 2002
BfuAI ACCTGC 2 cut(s) 842, 1129
BfuI GTATCC 1 cut(s) 2002
BglI GCCNNNNNGGC 1 cut(s) 464
BglII AGATCT 1 cut(s) 2312
BisI GCNGC 6 cut(s) 5, 246, 465, 740, 1572, 2432
BlpI GCTNAGC 1 cut(s) 2002
BlsI GCNGC 6 cut(s) 6, 247, 466, 741, 1573, 2433
Bme1390I CCNGG 7 cut(s) 116, 663, 1058, 1153, 1439, 2051, 2136
Bme18I GGWCC 2 cut(s) 2138, 2189
BmgT120I GGNCC 7 cut(s) 25, 112, 362, 1349, 2138, 2189, 2358
BmiI GGNNCC 5 cut(s) 26, 47, 734, 1350, 2121
BmrFI CCNGG 7 cut(s) 116, 663, 1058, 1153, 1439, 2051, 2136
BmrI ACTGGG 2 cut(s) 44, 2477
BmsI GCATC 3 cut(s) 70, 751, 1923
BmuI ACTGGG 2 cut(s) 44, 2477
BoxI GACNNNNGTC 1 cut(s) 2260
BpiI GAAGAC 2 cut(s) 578, 2256
BpmI CTGGAG 4 cut(s) 620, 1174, 1524, 2072
Bpu10I CCTNAGC 2 cut(s) 993, 1875
Bpu1102I GCTNAGC 1 cut(s) 2002
Bpu14I TTCGAA 2 cut(s) 1580, 2024
Bsa29I ATCGAT 1 cut(s) 964
BsaBI GATNNNNATC 1 cut(s) 843
BsaI GGTCTC 3 cut(s) 7, 155, 1176
BsaWI WCCGGW 7 cut(s) 158, 335, 370, 377, 384, 2122, 2281
Bse118I RCCGGY 5 cut(s) 272, 293, 335, 377, 2211
Bse1I ACTGG 4 cut(s) 50, 637, 734, 2472
Bse21I CCTNAGG 1 cut(s) 2206
Bse3DI GCAATG 1 cut(s) 1728
Bse8I GATNNNNATC 1 cut(s) 843
BseAI TCCGGA 1 cut(s) 2122
BseBI CCWGG 7 cut(s) 116, 663, 1058, 1153, 1439, 2051, 2136
BseCI ATCGAT 1 cut(s) 964
BseGI GGATG 4 cut(s) 85, 844, 1184, 2081
BseJI GATNNNNATC 1 cut(s) 843
BseMI GCAATG 1 cut(s) 1728
BseMII CTCAG 7 cut(s) 471, 692, 707, 984, 1352, 2016, 2220
BseNI ACTGG 4 cut(s) 50, 637, 734, 2472
BseRI GAGGAG 3 cut(s) 1313, 2105, 2303
BseXI GCAGC 4 cut(s) 257, 751, 1558, 2418
Bsh1236I CGCG 2 cut(s) 430, 1953
Bsh1285I CGRYCG 4 cut(s) 336, 456, 2271, 2304
BshNI GGYRCC 1 cut(s) 732
BshTI ACCGGT 2 cut(s) 335, 377
BshVI ATCGAT 1 cut(s) 964
BsiEI CGRYCG 4 cut(s) 336, 456, 2271, 2304
BsiHKAI GWGCWC 2 cut(s) 1666, 1891
BsiWI CGTACG 1 cut(s) 425
BslFI GGGAC 3 cut(s) 680, 1456, 1636
BsmAI GTCTC 6 cut(s) 7, 155, 1176, 1295, 1498, 2242
BsmBI CGTCTC 2 cut(s) 1498, 2242
BsmFI GGGAC 3 cut(s) 680, 1456, 1636
BsmI GAATGC 1 cut(s) 1534
Bso31I GGTCTC 3 cut(s) 7, 155, 1176
Bsp119I TTCGAA 2 cut(s) 1580, 2024
Bsp1286I GDGCHC 3 cut(s) 50, 1666, 1891
Bsp13I TCCGGA 1 cut(s) 2122
Bsp1720I GCTNAGC 1 cut(s) 2002
Bsp19I CCATGG 1 cut(s) 459
BspACI CCGC 8 cut(s) 5, 167, 192, 430, 438, 464, 737, 2006
BspCNI CTCAG 7 cut(s) 472, 691, 708, 985, 1351, 2015, 2219
BspDI ATCGAT 1 cut(s) 964
BspEI TCCGGA 1 cut(s) 2122
BspFNI CGCG 2 cut(s) 430, 1953
BspHI TCATGA 2 cut(s) 1666, 1930
BspLI GGNNCC 5 cut(s) 26, 47, 734, 1350, 2121
BspMAI CTGCAG 1 cut(s) 1126
BspMI ACCTGC 2 cut(s) 842, 1129
BspPI GGATC 5 cut(s) 169, 969, 1803, 2114, 2127
BspT104I TTCGAA 2 cut(s) 1580, 2024
BspT107I GGYRCC 1 cut(s) 732
BspTNI GGTCTC 3 cut(s) 7, 155, 1176
BsrBI CCGCTC 1 cut(s) 194
BsrDI GCAATG 1 cut(s) 1728
BsrFI RCCGGY 5 cut(s) 272, 293, 335, 377, 2211
BsrI ACTGG 4 cut(s) 50, 637, 734, 2472
BssAI RCCGGY 5 cut(s) 272, 293, 335, 377, 2211
BssSI CACGAG 1 cut(s) 1501
BssT1I CCWWGG 7 cut(s) 459, 485, 500, 1011, 1044, 1280, 1812
Bst2BI CACGAG 1 cut(s) 1501
Bst2UI CCWGG 7 cut(s) 116, 663, 1058, 1153, 1439, 2051, 2136
Bst4CI ACNGT 6 cut(s) 612, 1070, 1289, 2168, 2259, 2305
Bst6I CTCTTC 2 cut(s) 5, 1195
BstBI TTCGAA 2 cut(s) 1580, 2024
BstC8I GCNNGC 7 cut(s) 85, 270, 990, 1277, 1955, 2213, 2392
BstDEI CTNAG 9 cut(s) 480, 619, 678, 716, 993, 1338, 1875, 2002, 2206
BstDSI CCRYGG 3 cut(s) 459, 528, 608
BstEII GGTNACC 2 cut(s) 373, 380
BstF5I GGATG 4 cut(s) 85, 844, 1184, 2081
BstFNI CGCG 2 cut(s) 430, 1953
BstH2I RGCGCY 1 cut(s) 2002
BstHHI GCGC 4 cut(s) 1275, 1953, 2001, 2217
BstMAI GTCTC 6 cut(s) 7, 155, 1176, 1295, 1498, 2242
BstMCI CGRYCG 4 cut(s) 336, 456, 2271, 2304
BstMWI GCNNNNNNNGC 4 cut(s) 464, 739, 1348, 1433
BstNI CCWGG 7 cut(s) 116, 663, 1058, 1153, 1439, 2051, 2136
BstNSI RCATGY 1 cut(s) 1961
BstPAI GACNNNNGTC 1 cut(s) 2260
BstPI GGTNACC 2 cut(s) 373, 380
BstSCI CCNGG 7 cut(s) 114, 661, 1056, 1151, 1437, 2049, 2134
BstSFI CTRYAG 3 cut(s) 1122, 1343, 1608
BstUI CGCG 2 cut(s) 430, 1953
BstV1I GCAGC 4 cut(s) 257, 751, 1558, 2418
BstV2I GAAGAC 2 cut(s) 578, 2256
BstX2I RGATCY 3 cut(s) 161, 2119, 2312
BstYI RGATCY 3 cut(s) 161, 2119, 2312
Bsu15I ATCGAT 1 cut(s) 964
Bsu36I CCTNAGG 1 cut(s) 2206
BsuI GTATCC 1 cut(s) 2002
BsuTUI ATCGAT 1 cut(s) 964
BtgI CCRYGG 3 cut(s) 459, 528, 608
BtgZI GCGATG 2 cut(s) 1722, 2212
BtsCI GGATG 4 cut(s) 85, 844, 1184, 2081
BtsIMutI CAGTG 2 cut(s) 869, 1285
BveI ACCTGC 2 cut(s) 842, 1129
Cac8I GCNNGC 7 cut(s) 85, 270, 990, 1277, 1955, 2213, 2392
CaiI CAGNNNCTG 1 cut(s) 1130
CciI TCATGA 2 cut(s) 1666, 1930
CfoI GCGC 4 cut(s) 1275, 1953, 2001, 2217
Cfr10I RCCGGY 5 cut(s) 272, 293, 335, 377, 2211
Cfr13I GGNCC 7 cut(s) 25, 112, 362, 1349, 2138, 2189, 2358
ClaI ATCGAT 1 cut(s) 964
CseI GACGC 1 cut(s) 435
CsiI ACCWGGT 1 cut(s) 2134
Csp6I GTAC 3 cut(s) 426, 1206, 2132
CspAI ACCGGT 2 cut(s) 335, 377
CviAII CATG 9 cut(s) 460, 1109, 1232, 1373, 1667, 1820, 1931, 1958, 2276
CviQI GTAC 3 cut(s) 426, 1206, 2132
DdeI CTNAG 9 cut(s) 480, 619, 678, 716, 993, 1338, 1875, 2002, 2206
EaeI YGGCCR 5 cut(s) 266, 319, 525, 2199, 2346
Eam1104I CTCTTC 2 cut(s) 5, 1195
EarI CTCTTC 2 cut(s) 5, 1195
EciI GGCGGA 2 cut(s) 156, 427
Ecl136II GAGCTC 1 cut(s) 1664
Eco130I CCWWGG 7 cut(s) 459, 485, 500, 1011, 1044, 1280, 1812
Eco147I AGGCCT 1 cut(s) 1279
Eco24I GRGCYC 2 cut(s) 50, 1666
Eco31I GGTCTC 3 cut(s) 7, 155, 1176
Eco32I GATATC 1 cut(s) 1756
Eco47I GGWCC 2 cut(s) 2138, 2189
Eco53kI GAGCTC 1 cut(s) 1664
Eco57I CTGAAG 3 cut(s) 21, 566, 1176
Eco81I CCTNAGG 1 cut(s) 2206
Eco91I GGTNACC 2 cut(s) 373, 380
EcoICRI GAGCTC 1 cut(s) 1664
EcoO109I RGGNCCY 2 cut(s) 25, 1349
EcoO65I GGTNACC 2 cut(s) 373, 380
EcoRI GAATTC 1 cut(s) 283
EcoRII CCWGG 7 cut(s) 114, 661, 1056, 1151, 1437, 2049, 2134
EcoRV GATATC 1 cut(s) 1756
EcoT14I CCWWGG 7 cut(s) 459, 485, 500, 1011, 1044, 1280, 1812
EcoT38I GRGCYC 2 cut(s) 50, 1666
ErhI CCWWGG 7 cut(s) 459, 485, 500, 1011, 1044, 1280, 1812
Esp3I CGTCTC 2 cut(s) 1498, 2242
FaeI CATG 9 cut(s) 463, 1112, 1235, 1376, 1670, 1823, 1934, 1961, 2279
FaqI GGGAC 3 cut(s) 680, 1456, 1636
FatI CATG 9 cut(s) 459, 1108, 1231, 1372, 1666, 1819, 1930, 1957, 2275
FauI CCCGC 3 cut(s) 199, 423, 744
FblI GTMKAC 2 cut(s) 1728, 1808
Fnu4HI GCNGC 6 cut(s) 5, 246, 465, 740, 1572, 2432
FokI GGATG 4 cut(s) 92, 851, 1171, 2068
FriOI GRGCYC 2 cut(s) 50, 1666
Fsp4HI GCNGC 6 cut(s) 5, 246, 465, 740, 1572, 2432
FspBI CTAG 4 cut(s) 825, 848, 1424, 1631
FspI TGCGCA 1 cut(s) 1274
GlaI GCGC 4 cut(s) 1274, 1952, 2000, 2216
GluI GCNGC 6 cut(s) 5, 246, 465, 740, 1572, 2432
GsuI CTGGAG 4 cut(s) 620, 1174, 1524, 2072
HaeII RGCGCY 1 cut(s) 2002
HgaI GACGC 1 cut(s) 435
HhaI GCGC 4 cut(s) 1275, 1953, 2001, 2217
Hin1II CATG 9 cut(s) 463, 1112, 1235, 1376, 1670, 1823, 1934, 1961, 2279
Hin6I GCGC 4 cut(s) 1273, 1951, 1999, 2215
HinP1I GCGC 4 cut(s) 1273, 1951, 1999, 2215
HincII GTYRAC 3 cut(s) 1105, 1464, 1729
HindII GTYRAC 3 cut(s) 1105, 1464, 1729
HindIII AAGCTT 1 cut(s) 1410
HpaI GTTAAC 1 cut(s) 1464
HphI GGTGA 9 cut(s) 287, 308, 350, 385, 392, 866, 1325, 1652, 1853
Hpy166II GTNNAC 9 cut(s) 608, 971, 1031, 1054, 1105, 1464, 1729, 1809, 2132
Hpy188III TCNNGA 9 cut(s) 722, 1430, 1541, 1667, 1931, 2123, 2141, 2192, 2253
Hpy8I GTNNAC 9 cut(s) 608, 971, 1031, 1054, 1105, 1464, 1729, 1809, 2132
Hpy99I CGWCG 6 cut(s) 59, 101, 451, 526, 2245, 2248
HpyAV CCTTC 5 cut(s) 38, 549, 2242, 2362, 2440
HpyCH4III ACNGT 6 cut(s) 612, 1070, 1289, 2168, 2259, 2305
HpyCH4IV ACGT 2 cut(s) 1204, 1483
HpyCH4V TGCA 5 cut(s) 781, 1124, 1559, 1747, 2279
HpyF10VI GCNNNNNNNGC 4 cut(s) 464, 739, 1348, 1433
HpyF3I CTNAG 9 cut(s) 480, 619, 678, 716, 993, 1338, 1875, 2002, 2206
HpySE526I ACGT 2 cut(s) 1204, 1483
Hsp92II CATG 9 cut(s) 463, 1112, 1235, 1376, 1670, 1823, 1934, 1961, 2279
HspAI GCGC 4 cut(s) 1273, 1951, 1999, 2215
Kpn2I TCCGGA 1 cut(s) 2122
KroI GCCGGC 1 cut(s) 2211
KroNI GCCGGC 1 cut(s) 2213
KspAI GTTAAC 1 cut(s) 1464
LmnI GCTCC 6 cut(s) 45, 199, 1162, 1661, 2327, 2439
Lsp1109I GCAGC 4 cut(s) 257, 751, 1558, 2418
LweI GCATC 3 cut(s) 70, 751, 1923
MabI ACCWGGT 1 cut(s) 2134
MaeI CTAG 4 cut(s) 825, 848, 1424, 1631
MaeII ACGT 2 cut(s) 1204, 1483
MaeIII GTNAC 7 cut(s) 373, 380, 1134, 1447, 1841, 2044, 2250
MbiI CCGCTC 1 cut(s) 194
MboII GAAGA 6 cut(s) 22, 583, 1182, 2256, 2322, 2374
MfeI CAATTG 2 cut(s) 782, 903
MflI RGATCY 3 cut(s) 161, 2119, 2312
MhlI GDGCHC 3 cut(s) 50, 1666, 1891
MlsI TGGCCA 1 cut(s) 2201
MluNI TGGCCA 1 cut(s) 2201
MlyI GAGTC 4 cut(s) 382, 613, 727, 1618
MmeI TCCRAC 3 cut(s) 79, 928, 2156
Mox20I TGGCCA 1 cut(s) 2201
MroI TCCGGA 1 cut(s) 2122
MroNI GCCGGC 1 cut(s) 2211
MscI TGGCCA 1 cut(s) 2201
MslI CAYNNNNRTG 3 cut(s) 1377, 1935, 2257
Msp20I TGGCCA 1 cut(s) 2201
MspA1I CMGCKG 1 cut(s) 2006
MspR9I CCNGG 7 cut(s) 116, 663, 1058, 1153, 1439, 2051, 2136
MunI CAATTG 2 cut(s) 782, 903
Mva1269I GAATGC 1 cut(s) 1534
MvaI CCWGG 7 cut(s) 116, 663, 1058, 1153, 1439, 2051, 2136
MvnI CGCG 2 cut(s) 430, 1953
MwoI GCNNNNNNNGC 4 cut(s) 464, 739, 1348, 1433
NaeI GCCGGC 1 cut(s) 2213
NcoI CCATGG 1 cut(s) 459
NgoMIV GCCGGC 1 cut(s) 2211
NlaIII CATG 9 cut(s) 463, 1112, 1235, 1376, 1670, 1823, 1934, 1961, 2279
NlaIV GGNNCC 5 cut(s) 26, 47, 734, 1350, 2121
NmeAIII GCCGAG 1 cut(s) 284
NmuCI GTSAC 4 cut(s) 373, 380, 1841, 2250
NsbI TGCGCA 1 cut(s) 1274
NspI RCATGY 1 cut(s) 1961
NspV TTCGAA 2 cut(s) 1580, 2024
OliI CACNNNNGTG 1 cut(s) 2257
PagI TCATGA 2 cut(s) 1666, 1930
PaqCI CACCTGC 1 cut(s) 842
PceI AGGCCT 1 cut(s) 1279
PcsI WCGNNNNNNNCGW 3 cut(s) 60, 1513, 2246
PctI GAATGC 1 cut(s) 1534
PdiI GCCGGC 1 cut(s) 2213
PfeI GAWTC 7 cut(s) 304, 325, 346, 367, 514, 887, 1325
Pfl23II CGTACG 1 cut(s) 425
PflFI GACNNNGTC 1 cut(s) 717
PflMI CCANNNNNTGG 2 cut(s) 1444, 2355
PfoI TCCNGGA 1 cut(s) 1151
PinAI ACCGGT 2 cut(s) 335, 377
PkrI GCNGC 6 cut(s) 6, 247, 466, 741, 1573, 2433
Ple19I CGATCG 2 cut(s) 456, 2271
PleI GAGTC 4 cut(s) 382, 612, 726, 1618
PpsI GAGTC 4 cut(s) 382, 612, 726, 1618
PshAI GACNNNNGTC 1 cut(s) 2260
PshBI ATTAAT 1 cut(s) 1251
Psp124BI GAGCTC 1 cut(s) 1666
Psp6I CCWGG 7 cut(s) 114, 661, 1056, 1151, 1437, 2049, 2134
PspEI GGTNACC 2 cut(s) 373, 380
PspGI CCWGG 7 cut(s) 114, 661, 1056, 1151, 1437, 2049, 2134
PspLI CGTACG 1 cut(s) 425
PspN4I GGNNCC 5 cut(s) 26, 47, 734, 1350, 2121
PspPI GGNCC 7 cut(s) 25, 112, 362, 1349, 2138, 2189, 2358
PsrI GAACNNNNNNTAC 2 cut(s) 1119, 1151
PstI CTGCAG 1 cut(s) 1126
PstNI CAGNNNCTG 1 cut(s) 1130
PsuI RGATCY 3 cut(s) 161, 2119, 2312
PsyI GACNNNGTC 1 cut(s) 717
PvuI CGATCG 2 cut(s) 456, 2271
RsaI GTAC 3 cut(s) 427, 1207, 2133
RsaNI GTAC 3 cut(s) 426, 1206, 2132
RseI CAYNNNNRTG 3 cut(s) 1377, 1935, 2257
SacI GAGCTC 1 cut(s) 1666
SalI GTCGAC 1 cut(s) 1727
SatI GCNGC 6 cut(s) 5, 246, 465, 740, 1572, 2432
Sau96I GGNCC 7 cut(s) 25, 112, 362, 1349, 2138, 2189, 2358
SchI GAGTC 4 cut(s) 382, 613, 727, 1618
ScrFI CCNGG 7 cut(s) 116, 663, 1058, 1153, 1439, 2051, 2136
SduI GDGCHC 3 cut(s) 50, 1666, 1891
SexAI ACCWGGT 1 cut(s) 2134
SfaNI GCATC 3 cut(s) 70, 751, 1923
SfcI CTRYAG 3 cut(s) 1122, 1343, 1608
SfuI TTCGAA 2 cut(s) 1580, 2024
SinI GGWCC 2 cut(s) 2138, 2189
SmiMI CAYNNNNRTG 3 cut(s) 1377, 1935, 2257
SpeI ACTAGT 1 cut(s) 1630
SseBI AGGCCT 1 cut(s) 1279
SsiI CCGC 8 cut(s) 5, 167, 192, 430, 438, 464, 737, 2006
SspI AATATT 1 cut(s) 1945
SspMI CTAG 4 cut(s) 825, 848, 1424, 1631
SstI GAGCTC 1 cut(s) 1666
StuI AGGCCT 1 cut(s) 1279
StyD4I CCNGG 7 cut(s) 114, 661, 1056, 1151, 1437, 2049, 2134
StyI CCWWGG 7 cut(s) 459, 485, 500, 1011, 1044, 1280, 1812
TaaI ACNGT 6 cut(s) 612, 1070, 1289, 2168, 2259, 2305
TaiI ACGT 2 cut(s) 1207, 1486
TaqII GACCGA 1 cut(s) 1881
TauI GCSGC 2 cut(s) 7, 467
TfiI GAWTC 7 cut(s) 304, 325, 346, 367, 514, 887, 1325
TscAI CASTG 2 cut(s) 876, 1292
TseFI GTSAC 4 cut(s) 373, 380, 1841, 2250
TseI GCWGC 4 cut(s) 245, 739, 1571, 2431
Tsp45I GTSAC 4 cut(s) 373, 380, 1841, 2250
TspGWI ACGGA 3 cut(s) 545, 1505, 2234
TspRI CASTG 2 cut(s) 876, 1292
Tth111I GACNNNGTC 1 cut(s) 717
Van91I CCANNNNNTGG 2 cut(s) 1444, 2355
VpaK11BI GGWCC 2 cut(s) 2138, 2189
VspI ATTAAT 1 cut(s) 1251
XapI RAATTY 4 cut(s) 283, 809, 1673, 1712
XceI RCATGY 1 cut(s) 1961
XmiI GTMKAC 2 cut(s) 1728, 1808
XspI CTAG 4 cut(s) 825, 848, 1424, 1631
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.