Rorug05G0392400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0002163
Physical Location & Seq
Forward (+)
53670859 .. 53671248
390 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0392400.1

Sequence Viewer

Length: 390 bp
ATGAATGAAGCTGTCGACATAGAAAATGTGCACCCAGCTGGGAATGTACATGCGGAGCCCTGCATCTACAGAGTTCCTGAACGACTACGGAGTGTAAATGAAAATGCTTACACACCTCAGGCAGTCTCTATAGGTCCATTTCATCGCGGCACGGGAGGCTTGATAGTCACGGTAGAGGACAAAGACAACTACTTACAAAACCTTACTCCATTTGGGACCGAAGGGATAGTGAGGGGCCAGGAAGCAAGACTTCGCAATTGTTACGCAGACACCACTGATCAGTTTAGCAGTGATGACTTTGTGACAATGATTCTGAAAGATGCCAGATTCATTATTGAGGTATTGTTTCCCTACGTACATTGCAACATGACAACAATGATACCATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

129

Amino Acids

14.49

Weight (kDa)

4.91

Isoelectric Point (pI)

31.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 22 - 117 9.2e-22 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 15
AccII CGCG 1 cut(s) 147
AciI CCGC 2 cut(s) 53, 147
AfaI GTAC 2 cut(s) 48, 357
AjnI CCWGG 1 cut(s) 237
AluBI AGCT 2 cut(s) 11, 38
AluI AGCT 2 cut(s) 11, 38
Alw21I GWGCWC 1 cut(s) 33
Alw26I GTCTC 1 cut(s) 130
Alw44I GTGCAC 1 cut(s) 29
AoxI GGCC 1 cut(s) 235
ApaLI GTGCAC 1 cut(s) 29
AspS9I GGNCC 3 cut(s) 134, 216, 235
AvaII GGWCC 2 cut(s) 134, 216
AxyI CCTNAGG 1 cut(s) 117
BaeGI GKGCMC 1 cut(s) 33
BanII GRGCYC 1 cut(s) 60
Bbv12I GWGCWC 1 cut(s) 33
BciT130I CCWGG 1 cut(s) 239
BclI TGATCA 1 cut(s) 277
BcoDI GTCTC 1 cut(s) 130
BfmI CTRYAG 2 cut(s) 67, 129
BisI GCNGC 1 cut(s) 148
BlsI GCNGC 1 cut(s) 149
Bme1390I CCNGG 1 cut(s) 239
Bme18I GGWCC 2 cut(s) 134, 216
BmgT120I GGNCC 3 cut(s) 134, 216, 235
BmiI GGNNCC 3 cut(s) 57, 217, 236
BmrFI CCNGG 1 cut(s) 239
BmsI GCATC 2 cut(s) 72, 310
BsaAI YACGTR 1 cut(s) 355
Bse21I CCTNAGG 1 cut(s) 117
Bse3DI GCAATG 1 cut(s) 358
BseBI CCWGG 1 cut(s) 239
BseMI GCAATG 1 cut(s) 358
BseMII CTCAG 1 cut(s) 131
BseSI GKGCMC 1 cut(s) 33
BseYI CCCAGC 2 cut(s) 34, 38
Bsh1236I CGCG 1 cut(s) 147
BshFI GGCC 1 cut(s) 237
BsiHKAI GWGCWC 1 cut(s) 33
BslFI GGGAC 1 cut(s) 229
BsmAI GTCTC 1 cut(s) 130
BsmFI GGGAC 1 cut(s) 229
BsnI GGCC 1 cut(s) 237
Bsp1286I GDGCHC 2 cut(s) 33, 60
Bsp1407I TGTACA 1 cut(s) 46
Bsp143I GATC 1 cut(s) 277
BspACI CCGC 2 cut(s) 53, 147
BspANI GGCC 1 cut(s) 237
BspCNI CTCAG 1 cut(s) 130
BspFNI CGCG 1 cut(s) 147
BspLI GGNNCC 3 cut(s) 57, 217, 236
BsrDI GCAATG 1 cut(s) 358
BsrGI TGTACA 1 cut(s) 46
BssMI GATC 1 cut(s) 277
Bst2UI CCWGG 1 cut(s) 239
Bst4CI ACNGT 1 cut(s) 172
BstAUI TGTACA 1 cut(s) 46
BstBAI YACGTR 1 cut(s) 355
BstDEI CTNAG 1 cut(s) 117
BstFNI CGCG 1 cut(s) 147
BstKTI GATC 1 cut(s) 280
BstMAI GTCTC 1 cut(s) 130
BstMBI GATC 1 cut(s) 277
BstMWI GCNNNNNNNGC 1 cut(s) 156
BstNI CCWGG 1 cut(s) 239
BstNSI RCATGY 1 cut(s) 53
BstSCI CCNGG 1 cut(s) 237
BstSFI CTRYAG 2 cut(s) 67, 129
BstSLI GKGCMC 1 cut(s) 33
BstSNI TACGTA 1 cut(s) 355
BstUI CGCG 1 cut(s) 147
Bsu36I CCTNAGG 1 cut(s) 117
BsuRI GGCC 1 cut(s) 237
BtgZI GCGATG 1 cut(s) 128
BtsI GCAGTG 1 cut(s) 295
BtsIMutI CAGTG 2 cut(s) 273, 295
Cfr13I GGNCC 3 cut(s) 134, 216, 235
Csp6I GTAC 2 cut(s) 47, 356
CviAII CATG 3 cut(s) 50, 367, 384
CviJI RGCY 5 cut(s) 11, 38, 58, 159, 237
CviKI_1 RGCY 5 cut(s) 11, 38, 58, 159, 237
CviQI GTAC 2 cut(s) 47, 356
DdeI CTNAG 1 cut(s) 117
DpnI GATC 1 cut(s) 279
DpnII GATC 1 cut(s) 277
Eco105I TACGTA 1 cut(s) 355
Eco24I GRGCYC 1 cut(s) 60
Eco47I GGWCC 2 cut(s) 134, 216
Eco81I CCTNAGG 1 cut(s) 117
EcoRII CCWGG 1 cut(s) 237
EcoT38I GRGCYC 1 cut(s) 60
FaeI CATG 3 cut(s) 53, 370, 387
FaiI YATR 5 cut(s) 20, 51, 131, 368, 385
FalI AAGNNNNNCTT 2 cut(s) 234, 266
FaqI GGGAC 1 cut(s) 229
FatI CATG 3 cut(s) 49, 366, 383
FbaI TGATCA 1 cut(s) 277
FblI GTMKAC 1 cut(s) 15
Fnu4HI GCNGC 1 cut(s) 148
FriOI GRGCYC 1 cut(s) 60
Fsp4HI GCNGC 1 cut(s) 148
GluI GCNGC 1 cut(s) 148
GsaI CCCAGC 2 cut(s) 38, 42
HaeIII GGCC 1 cut(s) 237
Hin1II CATG 3 cut(s) 53, 370, 387
HincII GTYRAC 1 cut(s) 16
HindII GTYRAC 1 cut(s) 16
HinfI GANTC 2 cut(s) 310, 327
Hpy166II GTNNAC 2 cut(s) 16, 31
Hpy188I TCNGA 1 cut(s) 315
Hpy188III TCNNGA 1 cut(s) 77
Hpy8I GTNNAC 2 cut(s) 16, 31
HpyAV CCTTC 1 cut(s) 215
HpyCH4III ACNGT 1 cut(s) 172
HpyCH4IV ACGT 1 cut(s) 354
HpyCH4V TGCA 3 cut(s) 31, 63, 363
HpyF10VI GCNNNNNNNGC 1 cut(s) 156
HpyF3I CTNAG 1 cut(s) 117
HpySE526I ACGT 1 cut(s) 354
Hsp92II CATG 3 cut(s) 53, 370, 387
Ksp22I TGATCA 1 cut(s) 277
Kzo9I GATC 1 cut(s) 277
LmnI GCTCC 1 cut(s) 55
LpnPI CCDG 8 cut(s) 24, 48, 73, 90, 104, 224, 251, 337
LweI GCATC 2 cut(s) 72, 310
MaeII ACGT 1 cut(s) 354
MaeIII GTNAC 3 cut(s) 166, 260, 301
MalI GATC 1 cut(s) 279
MboI GATC 1 cut(s) 277
MfeI CAATTG 1 cut(s) 256
MhlI GDGCHC 2 cut(s) 33, 60
MluCI AATT 1 cut(s) 256
MnlI CCTC 5 cut(s) 126, 149, 169, 225, 331
MspA1I CMGCKG 1 cut(s) 38
MspR9I CCNGG 1 cut(s) 239
MunI CAATTG 1 cut(s) 256
MvaI CCWGG 1 cut(s) 239
MvnI CGCG 1 cut(s) 147
MwoI GCNNNNNNNGC 1 cut(s) 156
NdeII GATC 1 cut(s) 277
NlaIII CATG 3 cut(s) 53, 370, 387
NlaIV GGNNCC 3 cut(s) 57, 217, 236
NmuCI GTSAC 2 cut(s) 166, 301
NspI RCATGY 1 cut(s) 53
PfeI GAWTC 2 cut(s) 310, 327
PkrI GCNGC 1 cut(s) 149
Ppu21I YACGTR 1 cut(s) 355
Psp6I CCWGG 1 cut(s) 237
PspFI CCCAGC 2 cut(s) 34, 38
PspGI CCWGG 1 cut(s) 237
PspN4I GGNNCC 3 cut(s) 57, 217, 236
PspPI GGNCC 3 cut(s) 134, 216, 235
PvuII CAGCTG 1 cut(s) 38
RsaI GTAC 2 cut(s) 48, 357
RsaNI GTAC 2 cut(s) 47, 356
SalI GTCGAC 1 cut(s) 14
SatI GCNGC 1 cut(s) 148
Sau3AI GATC 1 cut(s) 277
Sau96I GGNCC 3 cut(s) 134, 216, 235
ScrFI CCNGG 1 cut(s) 239
SduI GDGCHC 2 cut(s) 33, 60
SetI ASST 7 cut(s) 13, 40, 118, 136, 204, 342, 357
SfaNI GCATC 2 cut(s) 72, 310
SfcI CTRYAG 2 cut(s) 67, 129
SinI GGWCC 2 cut(s) 134, 216
SnaBI TACGTA 1 cut(s) 355
Sse9I AATT 1 cut(s) 256
SsiI CCGC 2 cut(s) 53, 147
StyD4I CCNGG 1 cut(s) 237
TaaI ACNGT 1 cut(s) 172
TaiI ACGT 1 cut(s) 357
TaqI TCGA 1 cut(s) 15
TaqII GACCGA 1 cut(s) 233
TasI AATT 1 cut(s) 256
TatI WGTACW 1 cut(s) 46
TauI GCSGC 1 cut(s) 150
TfiI GAWTC 2 cut(s) 310, 327
TscAI CASTG 2 cut(s) 280, 295
TseFI GTSAC 2 cut(s) 166, 301
Tsp45I GTSAC 2 cut(s) 166, 301
TspDTI ATGAA 5 cut(s) 17, 21, 114, 131, 319
TspGWI ACGGA 1 cut(s) 103
TspRI CASTG 2 cut(s) 280, 295
VneI GTGCAC 1 cut(s) 29
VpaK11BI GGWCC 2 cut(s) 134, 216
XceI RCATGY 1 cut(s) 53
XmiI GTMKAC 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.