Transposase-associated domain
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1600 |
| AccII | CGCG | 1 cut(s) | 875 |
| AciI | CCGC | 3 cut(s) | 875, 1253, 1589 |
| AclI | AACGTT | 1 cut(s) | 553 |
| AclWI | GGATC | 5 cut(s) | 298, 506, 519, 566, 595 |
| AcoI | YGGCCR | 2 cut(s) | 58, 1533 |
| AcsI | RAATTY | 2 cut(s) | 643, 1410 |
| AcuI | CTGAAG | 1 cut(s) | 576 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 114, 725, 1369, 1600 |
| AgeI | ACCGGT | 1 cut(s) | 1080 |
| AjiI | CACGTC | 1 cut(s) | 1505 |
| AjnI | CCWGG | 3 cut(s) | 583, 712, 1405 |
| AluBI | AGCT | 1 cut(s) | 1382 |
| AluI | AGCT | 1 cut(s) | 1382 |
| Alw21I | GWGCWC | 2 cut(s) | 198, 546 |
| Alw26I | GTCTC | 3 cut(s) | 113, 1089, 1671 |
| AlwI | GGATC | 5 cut(s) | 298, 506, 519, 566, 595 |
| AoxI | GGCC | 4 cut(s) | 58, 745, 1533, 1694 |
| ApeKI | GCWGC | 1 cut(s) | 1232 |
| ApoI | RAATTY | 2 cut(s) | 643, 1410 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 418, 450 |
| AsiGI | ACCGGT | 1 cut(s) | 1080 |
| AspS9I | GGNCC | 7 cut(s) | 537, 623, 716, 728, 746, 1006, 1591 |
| AsuHPI | GGTGA | 2 cut(s) | 560, 1072 |
| AvaII | GGWCC | 6 cut(s) | 537, 623, 716, 728, 1006, 1591 |
| AxyI | CCTNAGG | 1 cut(s) | 968 |
| BamHI | GGATCC | 1 cut(s) | 511 |
| BauI | CACGAG | 1 cut(s) | 99 |
| Bbv12I | GWGCWC | 2 cut(s) | 198, 546 |
| BbvCI | CCTCAGC | 1 cut(s) | 1335 |
| BbvI | GCAGC | 1 cut(s) | 1244 |
| BccI | CCATC | 7 cut(s) | 497, 625, 739, 1051, 1073, 1170, 1504 |
| BceAI | ACGGC | 4 cut(s) | 45, 228, 1681, 1732 |
| BciT130I | CCWGG | 3 cut(s) | 585, 714, 1407 |
| BciVI | GTATCC | 1 cut(s) | 1630 |
| BclI | TGATCA | 1 cut(s) | 787 |
| BcoDI | GTCTC | 3 cut(s) | 113, 1089, 1671 |
| BfaI | CTAG | 3 cut(s) | 750, 755, 1128 |
| BfmI | CTRYAG | 1 cut(s) | 474 |
| BfuI | GTATCC | 1 cut(s) | 1630 |
| BglII | AGATCT | 1 cut(s) | 24 |
| BisI | GCNGC | 1 cut(s) | 1233 |
| BlpI | GCTNAGC | 1 cut(s) | 1283 |
| BlsI | GCNGC | 1 cut(s) | 1234 |
| Bme1390I | CCNGG | 3 cut(s) | 585, 714, 1407 |
| Bme18I | GGWCC | 6 cut(s) | 537, 623, 716, 728, 1006, 1591 |
| BmgBI | CACGTC | 1 cut(s) | 1505 |
| BmgT120I | GGNCC | 7 cut(s) | 537, 623, 716, 728, 746, 1006, 1591 |
| BmiI | GGNNCC | 3 cut(s) | 144, 513, 1079 |
| BmrFI | CCNGG | 3 cut(s) | 585, 714, 1407 |
| BmrI | ACTGGG | 1 cut(s) | 381 |
| BmsI | GCATC | 3 cut(s) | 499, 1057, 1219 |
| BmuI | ACTGGG | 1 cut(s) | 381 |
| BpmI | CTGGAG | 1 cut(s) | 1293 |
| Bpu10I | CCTNAGC | 2 cut(s) | 239, 1335 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1283 |
| BpuEI | CTTGAG | 3 cut(s) | 5, 150, 1200 |
| BsaBI | GATNNNNATC | 2 cut(s) | 612, 990 |
| BsaI | GGTCTC | 2 cut(s) | 113, 1089 |
| BsaJI | CCNNGG | 3 cut(s) | 244, 533, 742 |
| BsaWI | WCCGGW | 1 cut(s) | 1080 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1371, 1401 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 114, 725, 1369, 1600 |
| Bse118I | RCCGGY | 1 cut(s) | 1080 |
| Bse1I | ACTGG | 1 cut(s) | 376 |
| Bse21I | CCTNAGG | 1 cut(s) | 968 |
| Bse3DI | GCAATG | 1 cut(s) | 1742 |
| Bse8I | GATNNNNATC | 2 cut(s) | 612, 990 |
| BseBI | CCWGG | 3 cut(s) | 585, 714, 1407 |
| BseDI | CCNNGG | 3 cut(s) | 244, 533, 742 |
| BseGI | GGATG | 6 cut(s) | 279, 508, 731, 1062, 1402, 1519 |
| BseJI | GATNNNNATC | 2 cut(s) | 612, 990 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 114, 725, 1369, 1600 |
| BseMI | GCAATG | 1 cut(s) | 1742 |
| BseMII | CTCAG | 5 cut(s) | 306, 650, 915, 1326, 1691 |
| BseNI | ACTGG | 1 cut(s) | 376 |
| BseRI | GAGGAG | 2 cut(s) | 153, 206 |
| BseXI | GCAGC | 1 cut(s) | 1244 |
| BseYI | CCCAGC | 1 cut(s) | 1382 |
| Bsh1236I | CGCG | 1 cut(s) | 875 |
| BshFI | GGCC | 4 cut(s) | 60, 747, 1535, 1696 |
| BshTI | ACCGGT | 1 cut(s) | 1080 |
| BsiHKAI | GWGCWC | 2 cut(s) | 198, 546 |
| BsiSI | CCGG | 1 cut(s) | 1081 |
| BslFI | GGGAC | 2 cut(s) | 948, 1126 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 114, 725, 1369, 1600 |
| BsmAI | GTCTC | 3 cut(s) | 113, 1089, 1671 |
| BsmFI | GGGAC | 2 cut(s) | 948, 1126 |
| BsmI | GAATGC | 2 cut(s) | 88, 383 |
| BsnI | GGCC | 4 cut(s) | 60, 747, 1535, 1696 |
| Bso31I | GGTCTC | 2 cut(s) | 113, 1089 |
| Bsp1286I | GDGCHC | 2 cut(s) | 198, 546 |
| Bsp143I | GATC | 7 cut(s) | 24, 303, 511, 571, 587, 787, 991 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1283 |
| Bsp19I | CCATGG | 1 cut(s) | 742 |
| BspACI | CCGC | 3 cut(s) | 875, 1253, 1589 |
| BspANI | GGCC | 4 cut(s) | 60, 747, 1535, 1696 |
| BspCNI | CTCAG | 5 cut(s) | 307, 649, 916, 1327, 1690 |
| BspFNI | CGCG | 1 cut(s) | 875 |
| BspHI | TCATGA | 1 cut(s) | 1138 |
| BspLI | GGNNCC | 3 cut(s) | 144, 513, 1079 |
| BspMAI | CTGCAG | 1 cut(s) | 478 |
| BspPI | GGATC | 5 cut(s) | 298, 506, 519, 566, 595 |
| BspQI | GCTCTTC | 2 cut(s) | 480, 1416 |
| BspTNI | GGTCTC | 2 cut(s) | 113, 1089 |
| BsrDI | GCAATG | 1 cut(s) | 1742 |
| BsrFI | RCCGGY | 1 cut(s) | 1080 |
| BsrI | ACTGG | 1 cut(s) | 376 |
| BssAI | RCCGGY | 1 cut(s) | 1080 |
| BssECI | CCNNGG | 3 cut(s) | 244, 533, 742 |
| BssMI | GATC | 7 cut(s) | 24, 303, 511, 571, 587, 787, 991 |
| BssSI | CACGAG | 1 cut(s) | 99 |
| BssT1I | CCWWGG | 2 cut(s) | 533, 742 |
| Bst2BI | CACGAG | 1 cut(s) | 99 |
| Bst2UI | CCWGG | 3 cut(s) | 585, 714, 1407 |
| Bst4CI | ACNGT | 2 cut(s) | 356, 548 |
| Bst6I | CTCTTC | 4 cut(s) | 480, 1257, 1317, 1416 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1238 |
| BstC8I | GCNNGC | 1 cut(s) | 381 |
| BstDEI | CTNAG | 8 cut(s) | 239, 315, 636, 924, 968, 1283, 1335, 1677 |
| BstDSI | CCRYGG | 2 cut(s) | 244, 742 |
| BstEII | GGTNACC | 1 cut(s) | 1060 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 723 |
| BstF5I | GGATG | 6 cut(s) | 279, 508, 731, 1062, 1402, 1519 |
| BstFNI | CGCG | 1 cut(s) | 875 |
| BstKTI | GATC | 7 cut(s) | 27, 306, 514, 574, 590, 790, 994 |
| BstMAI | GTCTC | 3 cut(s) | 113, 1089, 1671 |
| BstMBI | GATC | 7 cut(s) | 24, 303, 511, 571, 587, 787, 991 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 1133, 1238 |
| BstNI | CCWGG | 3 cut(s) | 585, 714, 1407 |
| BstNSI | RCATGY | 2 cut(s) | 115, 410 |
| BstPI | GGTNACC | 1 cut(s) | 1060 |
| BstSCI | CCNGG | 3 cut(s) | 583, 712, 1405 |
| BstSFI | CTRYAG | 1 cut(s) | 474 |
| BstUI | CGCG | 1 cut(s) | 875 |
| BstV1I | GCAGC | 1 cut(s) | 1244 |
| BstX2I | RGATCY | 3 cut(s) | 24, 511, 587 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1176 |
| BstYI | RGATCY | 3 cut(s) | 24, 511, 587 |
| Bsu36I | CCTNAGG | 1 cut(s) | 968 |
| BsuI | GTATCC | 1 cut(s) | 1630 |
| BsuRI | GGCC | 4 cut(s) | 60, 747, 1535, 1696 |
| BtgI | CCRYGG | 2 cut(s) | 244, 742 |
| BtrI | CACGTC | 1 cut(s) | 1505 |
| BtsCI | GGATG | 6 cut(s) | 279, 508, 731, 1062, 1402, 1519 |
| BtsIMutI | CAGTG | 1 cut(s) | 361 |
| Cac8I | GCNNGC | 1 cut(s) | 381 |
| CciI | TCATGA | 1 cut(s) | 1138 |
| Cfr10I | RCCGGY | 1 cut(s) | 1080 |
| Cfr13I | GGNCC | 7 cut(s) | 537, 623, 716, 728, 746, 1006, 1591 |
| CspAI | ACCGGT | 1 cut(s) | 1080 |
| DdeI | CTNAG | 8 cut(s) | 239, 315, 636, 924, 968, 1283, 1335, 1677 |
| DpnI | GATC | 7 cut(s) | 26, 305, 513, 573, 589, 789, 993 |
| DpnII | GATC | 7 cut(s) | 24, 303, 511, 571, 587, 787, 991 |
| DraI | TTTAAA | 1 cut(s) | 1615 |
| EaeI | YGGCCR | 2 cut(s) | 58, 1533 |
| Eam1104I | CTCTTC | 4 cut(s) | 480, 1257, 1317, 1416 |
| EarI | CTCTTC | 4 cut(s) | 480, 1257, 1317, 1416 |
| Eco130I | CCWWGG | 2 cut(s) | 533, 742 |
| Eco31I | GGTCTC | 2 cut(s) | 113, 1089 |
| Eco47I | GGWCC | 6 cut(s) | 537, 623, 716, 728, 1006, 1591 |
| Eco57I | CTGAAG | 1 cut(s) | 576 |
| Eco81I | CCTNAGG | 1 cut(s) | 968 |
| Eco91I | GGTNACC | 1 cut(s) | 1060 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 723 |
| EcoO109I | RGGNCCY | 1 cut(s) | 716 |
| EcoO65I | GGTNACC | 1 cut(s) | 1060 |
| EcoRI | GAATTC | 1 cut(s) | 1410 |
| EcoRII | CCWGG | 3 cut(s) | 583, 712, 1405 |
| EcoT14I | CCWWGG | 2 cut(s) | 533, 742 |
| ErhI | CCWWGG | 2 cut(s) | 533, 742 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 159, 191 |
| FaqI | GGGAC | 2 cut(s) | 948, 1126 |
| FbaI | TGATCA | 1 cut(s) | 787 |
| Fnu4HI | GCNGC | 1 cut(s) | 1233 |
| FokI | GGATG | 6 cut(s) | 266, 515, 718, 1069, 1409, 1526 |
| Fsp4HI | GCNGC | 1 cut(s) | 1233 |
| FspBI | CTAG | 3 cut(s) | 750, 755, 1128 |
| GluI | GCNGC | 1 cut(s) | 1233 |
| GsaI | CCCAGC | 1 cut(s) | 1386 |
| GsuI | CTGGAG | 1 cut(s) | 1293 |
| HaeIII | GGCC | 4 cut(s) | 60, 747, 1535, 1696 |
| HapII | CCGG | 1 cut(s) | 1081 |
| HincII | GTYRAC | 2 cut(s) | 1107, 1297 |
| HindII | GTYRAC | 2 cut(s) | 1107, 1297 |
| HinfI | GANTC | 4 cut(s) | 596, 768, 870, 1099 |
| HpaII | CCGG | 1 cut(s) | 1081 |
| HphI | GGTGA | 2 cut(s) | 560, 1072 |
| Hpy166II | GTNNAC | 9 cut(s) | 104, 526, 551, 557, 802, 980, 1107, 1297, 1732 |
| Hpy188III | TCNNGA | 9 cut(s) | 22, 101, 167, 621, 886, 955, 1139, 1217, 1310 |
| Hpy8I | GTNNAC | 9 cut(s) | 104, 526, 551, 557, 802, 980, 1107, 1297, 1732 |
| HpyAV | CCTTC | 2 cut(s) | 719, 1348 |
| HpyCH4III | ACNGT | 2 cut(s) | 356, 548 |
| HpyCH4IV | ACGT | 2 cut(s) | 553, 1504 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 1133, 1238 |
| HpyF3I | CTNAG | 8 cut(s) | 239, 315, 636, 924, 968, 1283, 1335, 1677 |
| HpySE526I | ACGT | 2 cut(s) | 553, 1504 |
| Ksp22I | TGATCA | 1 cut(s) | 787 |
| Kzo9I | GATC | 7 cut(s) | 24, 303, 511, 571, 587, 787, 991 |
| LguI | GCTCTTC | 2 cut(s) | 480, 1416 |
| LmnI | GCTCC | 5 cut(s) | 193, 888, 1312, 1379, 1654 |
| Lsp1109I | GCAGC | 1 cut(s) | 1244 |
| LweI | GCATC | 3 cut(s) | 499, 1057, 1219 |
| MaeI | CTAG | 3 cut(s) | 750, 755, 1128 |
| MaeII | ACGT | 2 cut(s) | 553, 1504 |
| MaeIII | GTNAC | 4 cut(s) | 208, 847, 1060, 1387 |
| MalI | GATC | 7 cut(s) | 26, 305, 513, 573, 589, 789, 993 |
| MboI | GATC | 7 cut(s) | 24, 303, 511, 571, 587, 787, 991 |
| MfeI | CAATTG | 3 cut(s) | 561, 1224, 1248 |
| MflI | RGATCY | 3 cut(s) | 24, 511, 587 |
| MhlI | GDGCHC | 2 cut(s) | 198, 546 |
| MseI | TTAA | 6 cut(s) | 177, 233, 417, 612, 1193, 1614 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1143, 1203 |
| MspI | CCGG | 1 cut(s) | 1081 |
| MspR9I | CCNGG | 3 cut(s) | 585, 714, 1407 |
| MssI | GTTTAAAC | 1 cut(s) | 1615 |
| MunI | CAATTG | 3 cut(s) | 561, 1224, 1248 |
| Mva1269I | GAATGC | 2 cut(s) | 88, 383 |
| MvaI | CCWGG | 3 cut(s) | 585, 714, 1407 |
| MvnI | CGCG | 1 cut(s) | 875 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 1133, 1238 |
| NcoI | CCATGG | 1 cut(s) | 742 |
| NdeII | GATC | 7 cut(s) | 24, 303, 511, 571, 587, 787, 991 |
| NlaIV | GGNNCC | 3 cut(s) | 144, 513, 1079 |
| NmuCI | GTSAC | 3 cut(s) | 847, 1060, 1387 |
| NspI | RCATGY | 2 cut(s) | 115, 410 |
| PagI | TCATGA | 1 cut(s) | 1138 |
| PciSI | GCTCTTC | 2 cut(s) | 480, 1416 |
| PctI | GAATGC | 2 cut(s) | 88, 383 |
| PfeI | GAWTC | 4 cut(s) | 596, 768, 870, 1099 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1600 |
| PfoI | TCCNGGA | 1 cut(s) | 1405 |
| PinAI | ACCGGT | 1 cut(s) | 1080 |
| PkrI | GCNGC | 1 cut(s) | 1234 |
| PmeI | GTTTAAAC | 1 cut(s) | 1615 |
| PpuMI | RGGWCCY | 1 cut(s) | 716 |
| Psp1406I | AACGTT | 1 cut(s) | 553 |
| Psp5II | RGGWCCY | 1 cut(s) | 716 |
| Psp6I | CCWGG | 3 cut(s) | 583, 712, 1405 |
| PspEI | GGTNACC | 1 cut(s) | 1060 |
| PspFI | CCCAGC | 1 cut(s) | 1382 |
| PspGI | CCWGG | 3 cut(s) | 583, 712, 1405 |
| PspN4I | GGNNCC | 3 cut(s) | 144, 513, 1079 |
| PspPI | GGNCC | 7 cut(s) | 537, 623, 716, 728, 746, 1006, 1591 |
| PspPPI | RGGWCCY | 1 cut(s) | 716 |
| PsrI | GAACNNNNNNTAC | 1 cut(s) | 29 |
| PstI | CTGCAG | 1 cut(s) | 478 |
| PsuI | RGATCY | 3 cut(s) | 24, 511, 587 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1143, 1203 |
| SapI | GCTCTTC | 2 cut(s) | 480, 1416 |
| SaqAI | TTAA | 6 cut(s) | 177, 233, 417, 612, 1193, 1614 |
| SatI | GCNGC | 1 cut(s) | 1233 |
| Sau3AI | GATC | 7 cut(s) | 24, 303, 511, 571, 587, 787, 991 |
| Sau96I | GGNCC | 7 cut(s) | 537, 623, 716, 728, 746, 1006, 1591 |
| ScrFI | CCNGG | 3 cut(s) | 585, 714, 1407 |
| SduI | GDGCHC | 2 cut(s) | 198, 546 |
| SfaNI | GCATC | 3 cut(s) | 499, 1057, 1219 |
| SfcI | CTRYAG | 1 cut(s) | 474 |
| SinI | GGWCC | 6 cut(s) | 537, 623, 716, 728, 1006, 1591 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1143, 1203 |
| SmlI | CTYRAG | 3 cut(s) | 20, 165, 1215 |
| SmoI | CTYRAG | 3 cut(s) | 20, 165, 1215 |
| SsiI | CCGC | 3 cut(s) | 875, 1253, 1589 |
| SspMI | CTAG | 3 cut(s) | 750, 755, 1128 |
| StyD4I | CCNGG | 3 cut(s) | 583, 712, 1405 |
| StyI | CCWWGG | 2 cut(s) | 533, 742 |
| TaaI | ACNGT | 2 cut(s) | 356, 548 |
| TaiI | ACGT | 2 cut(s) | 556, 1507 |
| TaqII | GACCGA | 1 cut(s) | 554 |
| TfiI | GAWTC | 4 cut(s) | 596, 768, 870, 1099 |
| Tru1I | TTAA | 6 cut(s) | 177, 233, 417, 612, 1193, 1614 |
| Tru9I | TTAA | 6 cut(s) | 177, 233, 417, 612, 1193, 1614 |
| TscAI | CASTG | 1 cut(s) | 361 |
| TseFI | GTSAC | 3 cut(s) | 847, 1060, 1387 |
| TseI | GCWGC | 1 cut(s) | 1232 |
| Tsp45I | GTSAC | 3 cut(s) | 847, 1060, 1387 |
| TspGWI | ACGGA | 2 cut(s) | 426, 1307 |
| TspRI | CASTG | 1 cut(s) | 361 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1600 |
| VpaK11BI | GGWCC | 6 cut(s) | 537, 623, 716, 728, 1006, 1591 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 723 |
| XapI | RAATTY | 2 cut(s) | 643, 1410 |
| XceI | RCATGY | 2 cut(s) | 115, 410 |
| XspI | CTAG | 3 cut(s) | 750, 755, 1128 |