Rh5BG180300

Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000040
Physical Location & Seq
Forward (+)
19790764 .. 19811449
20686 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG180300.1

Sequence Viewer

Length: 2058 bp
ATGAAGGACAAGATTGACAAGATGAAGACTACAAAGAGCGCATCCCAACTTCCAGAGGTTCTTAATGATTTTGAAATCAATGAAGTATATGCGTATGTGCTTGGAAAACAGACACGTGGAGGGGTACGAGGATTTGGCATTGGAGTCAGACCAGAACAAGTTCCTGGTGTACTAGTGCAGAAAAGAGGTGTTCATTTAGAGGTACAAGCAATGAGAGAACAACATGAGGCTGAGATTGAAACGATTCGGAAGGAGAGCCAAGAAAAGGAAGATAAGCTTAGGGAGGAAATGAACAAACAAACTCTGGCGGCCACAAAAGATAAAATAAAAAATATGGAGATTGCAATGAAAAAACAGGAAGCTCTTATGATGGTGTTACTTGGGGGTGATTACGTAAATTCAATGAGAGCAGCACAGGAGTTCAGCTCAAGAATGTTTGATTCCATAAAGTCTCTAAGCATCGATCTGGGCTTTTTGACGTCTGTGGATCGGAGCCCGGTGATAAGTGATAACCCCATCGATCTTGAGATTGCTGAAAATGAAGAGGATATTGGAGAAATCTCAGTCATGTGGTACAATTTCTGCCTTAGCCCTGAAGAAATTGGCATCTTCATCATCTCAGGCCGCAGATTCTCTCAGATTATAAGATCAGACTACGCTGAAATGACTTCCCTTCAAATCTCCCCCAACACAATTTCTCAACCTCTCAATCTCCGCCGTCAATTCAGCTCAAACTTGGACCAAAGGGTCCGACTAATCAATGGCCGGAGACCCGTGATGGTTTGCCTTAGCCTTGACGTGGAACCACCAGTTACTGCATCAAAATCAGGGTTTTCGAAGAGCAAGGAGGTCATAGAGACAGAGGCGAAGGTTCTGGTGGGGACATACGCAAGAACTCCAGTGGTGTTGGCCAGTGGCAAAGGTTGTAAATTGTACGACATCGAGGGGAGAGAGTATTTGGATTTGAGCTCAGGGATTGCGGTGAATGCACTTGGACATGGTGATGAGGACTGGTTGAAGGCTGTGGTTGAACAGGCCAATACTCTCACTCATGTCAGCAATGTCTACTACTCCATCCCTCAGGTGGAACTAGCAGAGCGTTTGGTGGGCTCTTGTTTTGCAGACAGTGTGTTCTTCACTAATTCTGGAACTGAAGCAAACGAAGCAGCCATTAAATTTGCGAGGAAGTTTCAGAGACACAATCATCCCGATGCGAAAGAGCCTGCCACAAACTTCATATCTTTCACTAACAGCTTCCATGGCAGGACCATCGGTGCGCTTGCCTTGACTAGCAAAGAGTATTACAGGTCTCCGTTTGAGCCCGTCATGCCTGGAGTCACATTTGCAGAGTATGGAAATATAGAAGATACCAGAAAATTGATTCAGCCTGGAAAAACTGCTGCGGTTTTTGTTGAACCTATCCAGGGTGAAGGGGGCATATACAGTGCTACCAAGGAGTTTCTGCAATTTCTGCGTACTGCTTGTGATGAGGCTGGGGCTCTTCTGGTGTTCGACGAGGTTCAATGTGGTTTAGGCCGAACTGGGTATCTGTGGGCGCATGAGGCATTTGGTGTGTTCCCAGATATAATGACACTAGCTAAGCCACTAGCTGGAGGCCTACCCATTGGAGCTGCATTGGTGACTGAAAGAGTTGCTTTTGCCATAGCTTTCGGTGACCATGGAAGTACTTTTGCTGGTGCACCTCTCGTGTGCAATGCTGCCTTAGCCGTTCTCAAAAAGATATCAAATCCCAATTTTCTGGACAGTGTCTCTAAGAAAGGTCTCTACTTCAAACATATCTTAAATCAGAAGCTGGGAGGAAACCCTCATGTGAGAGAAATACGCGGTTTTGGGCTTATTATTGGAGTTGAATTGGATGTCCCTGCCTCACCTCTTGTGGATGCATGTAGAAGTGCCGGCCTTCTAGTGTTAACTGCTGGAAAAGGCAATGTTGTGAGGCTAGTGCCTCCATTGATCATAAGTGAGCAGGAGCTTGATCGTGCAGCTGAGATTTTGTACCAAGCGTTGCCTGTACTTGACGAAAAAAATTCAAATTAG

Protein Analysis

685

Amino Acids

75.29

Weight (kDa)

5.68

Isoelectric Point (pI)

42.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_3 PF00202 299 - 676 4.6e-112 Aminotransferase class-III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000040)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00150 FvH4_2g13531 FvH4_2g21543 FvH4_3g32800 FvH4_3g32802 FvH4_4g00091 FvH4_4g00092 FvH4_5g22070 FvH4_5g31391 FvH4_6g11351 FvH4_6g23430 FvH4_6g23672 FvH4_6g52753
malus_domestica MD03G1101500.v1.1 MD08G1234800.v1.1
pyrus_communis pycom07g24860 pycom09g07860 pycom10g09330 pycom10g15360 pycom11g01600 pycom11g13460 pycom13g15580
rosa_chinensis RchiOBHm_Chr1g0332021 RchiOBHm_Chr2g0095801 RchiOBHm_Chr2g0111771 RchiOBHm_Chr2g0127541 RchiOBHm_Chr2g0136581 RchiOBHm_Chr2g0136591 RchiOBHm_Chr2g0143311 RchiOBHm_Chr2g0145131 RchiOBHm_Chr2g0151391 RchiOBHm_Chr3g0468771 RchiOBHm_Chr3g0473281 RchiOBHm_Chr3g0481961 RchiOBHm_Chr4g0393741 RchiOBHm_Chr4g0397391 RchiOBHm_Chr4g0435991 RchiOBHm_Chr5g0015951 RchiOBHm_Chr5g0016651 RchiOBHm_Chr5g0039191 RchiOBHm_Chr5g0039211 RchiOBHm_Chr6g0254441 RchiOBHm_Chr6g0260911 RchiOBHm_Chr6g0274501 RchiOBHm_Chr6g0274511 RchiOBHm_Chr7g0184951 RchiOBHm_Chr7g0201701 RchiOBHm_Chr7g0201721 RchiOBHm_Chr7g0201731 RchiOBHm_Chr7g0234921 RchiOBHm_Chr7g0237841
rosa_laevigata RLG00000001204 RLG00000001230 RLG00000001341 RLG00000001513 RLG00000001971 RLG00000002177 RLG00000002360 RLG00000003112 RLG00000003130 RLG00000003854 RLG00000008209 RLG00000008322 RLG00000008412 RLG00000008524 RLG00000009491 RLG00000009908 RLG00000011823 RLG00000013252 RLG00000013782 RLG00000013944 RLG00000014480 RLG00000019084 RLG00000019544 RLG00000019751 RLG00000020093 RLG00000020094 RLG00000020551 RLG00000020838 RLG00000020982 RLG00000021059 RLG00000022067 RLG00000022604 RLG00000022632 RLG00000022849 RLG00000023962 RLG00000025767 RLG00000026838 RLG00000027291 RLG00000028131 RLG00000028210 RLG00000028834 RLG00000030169 RLG00000031271 RLG00000032327 RLG00000033996 RLG00000034546 RLG00000034654 RLG00000034810 RLG00000035087 RLG00000036782
rosa_multiflora Rmu_co8330487.1_g000001 Rmu_co8359977.1_g000001 Rmu_co8445341.1_g000001 Rmu_sc0000141.1_g000003 Rmu_sc0000353.1_g000018 Rmu_sc0000740.1_g000017 Rmu_sc0000744.1_g000138 Rmu_sc0000813.1_g000014 Rmu_sc0000908.1_g000003 Rmu_sc0000999.1_g000014 Rmu_sc0001103.1_g000001 Rmu_sc0001248.1_g000013 Rmu_sc0001282.1_g000021 Rmu_sc0001293.1_g000018 Rmu_sc0001608.1_g000015 Rmu_sc0001616.1_g000060 Rmu_sc0001851.1_g000013 Rmu_sc0001990.1_g000003 Rmu_sc0002138.1_g000002 Rmu_sc0002200.1_g000021 Rmu_sc0002230.1_g000029 Rmu_sc0002230.1_g000030 Rmu_sc0002702.1_g000017 Rmu_sc0002806.1_g000010 Rmu_sc0002809.1_g000023 Rmu_sc0002869.1_g000005 Rmu_sc0003156.1_g000027 Rmu_sc0003368.1_g000018 Rmu_sc0003642.1_g000007 Rmu_sc0003653.1_g000004 Rmu_sc0003791.1_g000008 Rmu_sc0003804.1_g000013 Rmu_sc0004015.1_g000022 Rmu_sc0004230.1_g000017 Rmu_sc0004368.1_g000011 Rmu_sc0004529.1_g000011 Rmu_sc0004540.1_g000011 Rmu_sc0005008.1_g000014 Rmu_sc0005171.1_g000001 Rmu_sc0005212.1_g000013 Rmu_sc0005947.1_g000003 Rmu_sc0006317.1_g000026 Rmu_sc0006578.1_g000005 Rmu_sc0006993.1_g000001 Rmu_sc0007442.1_g000002 Rmu_sc0007442.1_g000004 Rmu_sc0007876.1_g000004 Rmu_sc0007876.1_g000005 Rmu_sc0009624.1_g000004 Rmu_sc0009742.1_g000005 Rmu_sc0010306.1_g000007 Rmu_sc0011745.1_g000008 Rmu_sc0011800.1_g000003 Rmu_sc0012369.1_g000003 Rmu_sc0012787.1_g000003 Rmu_sc0012989.1_g000001 Rmu_sc0013416.1_g000003 Rmu_sc0021155.1_g000001 Rmu_sc0022372.1_g000001 Rmu_sc0023106.1_g000001 Rmu_sc0023684.1_g000003 Rmu_sc0023684.1_g000004 Rmu_sc0026897.1_g000001 Rmu_sc0027278.1_g000001 Rmu_sc0028533.1_g000001 Rmu_sc0028533.1_g000003 Rmu_sc0038440.1_g000001
rosa_roxburghii Rroxscaffold_1G00031730 Rroxscaffold_1G00033690 Rroxscaffold_1G00073100 Rroxscaffold_2G00079640 Rroxscaffold_2G00089760 Rroxscaffold_2G00091520 Rroxscaffold_2G00112420 Rroxscaffold_2G00115720 Rroxscaffold_2G00116170 Rroxscaffold_2G00116180 Rroxscaffold_2G00152590 Rroxscaffold_2G00155940 Rroxscaffold_3G00220010 Rroxscaffold_3G00234940 Rroxscaffold_3G00235250 Rroxscaffold_3G00237510 Rroxscaffold_3G00250950 Rroxscaffold_3G00250960 Rroxscaffold_3G00262030 Rroxscaffold_4G00299560 Rroxscaffold_4G00306000 Rroxscaffold_4G00311350 Rroxscaffold_4G00318530 Rroxscaffold_4G00329140 Rroxscaffold_5G00342020 Rroxscaffold_5G00350770 Rroxscaffold_5G00354620 Rroxscaffold_5G00354630 Rroxscaffold_5G00361180 Rroxscaffold_5G00377270 Rroxscaffold_6G00397870 Rroxscaffold_6G00397960 Rroxscaffold_6G00407290 Rroxscaffold_6G00408690 Rroxscaffold_6G00421900 Rroxscaffold_6G00421910 Rroxscaffold_7G00181630 Rroxscaffold_7G00182330 Rroxscaffold_7G00182340 Rroxscaffold_7G00212590 Rroxscaffold_7G00214060 Rroxscaffold_7G00214070 Rroxscaffold_7G00215940
rosa_rugosa Rorug01G0052800 Rorug01G0052800 Rorug01G0075200 Rorug01G0090500 Rorug01G0091500 Rorug01G0106000 Rorug01G0309300 Rorug01G0309400.1 Rorug02G0092000 Rorug02G0128200 Rorug02G0152800 Rorug02G0366100 Rorug02G0401500 Rorug02G0465600 Rorug02G0474400 Rorug03G0322500 Rorug04G0044000 Rorug04G0044100 Rorug04G0064800.1 Rorug04G0077400 Rorug04G0094800 Rorug04G0094800 Rorug04G0111400 Rorug04G0137800 Rorug04G0137900 Rorug04G0138000 Rorug04G0138000 Rorug04G0446200 Rorug05G0090600 Rorug05G0239900 Rorug05G0284400 Rorug05G0534400 Rorug05G0561600 Rorug06G0098100 Rorug06G0098100 Rorug06G0134500 Rorug06G0238500 Rorug06G0238600 Rorug07G0228900 Rorug07G0229000 Rorug07G0291600 Rorug07G0291700
rosa_samantha Rh1AG082200 Rh1AG129200 Rh1AG131500 Rh1AG159900 Rh1AG330800 Rh1AG408400 Rh1BG089600 Rh1CG086000 Rh1CG149200 Rh1CG405600 Rh1DG008200 Rh1DG015800 Rh1DG068000 Rh1DG118400 Rh1DG122500 Rh1DG202400 Rh1DG202500 Rh1DG266300 Rh1DG266400 Rh1DG287400 Rh1DG314700 Rh1DG314800 Rh2AG128400 Rh2AG128500 Rh2AG291000 Rh2AG347900 Rh2AG350700 Rh2AG415100 Rh2AG417200 Rh2AG497700 Rh2AG505000 Rh2AG507600 Rh2BG131300 Rh2BG233900 Rh2BG291700 Rh2BG291800 Rh2BG302700 Rh2BG302800 Rh2BG334700 Rh2BG334900 Rh2BG347000 Rh2BG347800 Rh2BG357100 Rh2BG528200 Rh2BG528300 Rh2BG603700 Rh2CG032300 Rh2CG034300 Rh2CG232600 Rh2CG483700 Rh2DG077400 Rh2DG103100 Rh2DG103200 Rh2DG287000 Rh2DG287200 Rh2DG375000 Rh2DG382700 Rh2DG537400 Rh2DG602900 Rh2DG603000 Rh3AG080800 Rh3AG217300 Rh3AG321800 Rh3BG082700 Rh3BG082800 Rh3BG168600 Rh3BG185900 Rh3BG224400 Rh3BG250500 Rh3BG260700 Rh3BG351300 Rh3BG353600 Rh3BG353700 Rh3CG082900 Rh3CG083000 Rh3CG176200 Rh3CG176300 Rh3CG219400 Rh3CG219500 Rh3CG254600 Rh3CG254700 Rh3CG256000 Rh3CG256100 Rh3CG257400 Rh3CG356200 Rh3CG356300 Rh3DG084300 Rh4AG030500 Rh4BG132300 Rh4BG139800 Rh4BG140000 Rh4CG027200 Rh4CG033100 Rh4CG033200 Rh4CG069600 Rh4CG103300 Rh4CG322600 Rh4DG028500 Rh4DG081000 Rh4DG190200 Rh4DG193500 Rh4DG212900 Rh4DG303900 Rh4DG342900 Rh5AG131000 Rh5AG166800 Rh5AG167000 Rh5AG201600 Rh5AG372200 Rh5AG372300 Rh5AG417900 Rh5BG082500 Rh5BG171200 Rh5BG171300 Rh5BG180300 Rh5BG194400 Rh5BG302800 Rh5BG343300 Rh5BG402700 Rh5BG428500 Rh5CG190700 Rh5CG190800 Rh5CG219100 Rh5CG451900 Rh5DG175200 Rh5DG175400 Rh5DG177000 Rh5DG177100 Rh5DG356000 Rh5DG356100 Rh5DG493500 Rh6AG006700 Rh6AG051700 Rh6AG064100 Rh6AG064200 Rh6AG064500 Rh6AG092800 Rh6AG152000 Rh6AG152100 Rh6AG291900 Rh6AG419000 Rh6AG465900 Rh6AG466000 Rh6BG125000 Rh6BG160600 Rh6BG161800 Rh6BG359100 Rh6BG359200 Rh6CG071400 Rh6CG071500 Rh6CG103500 Rh6CG103600 Rh6CG117200 Rh6CG123700 Rh6CG123800 Rh6CG156600 Rh6CG192500 Rh6CG208600 Rh6CG208700 Rh6CG365200 Rh6DG146200 Rh6DG146800 Rh6DG193200 Rh6DG193300 Rh6DG193400 Rh6DG349200 Rh7AG251100 Rh7AG259300 Rh7AG425100 Rh7BG262700 Rh7BG262900 Rh7BG369500 Rh7CG107400 Rh7CG107500 Rh7CG257400 Rh7CG267300 Rh7DG070500 Rh7DG105000 Rh7DG198500 Rh7DG291300 Rh7DG480600
rosa_wichuraiana Rw2G027920 Rw3G014750 Rw3G021490 Rw4G001910 Rw6G013120 Rw6G020040 Rw6G022930 Rw6G041900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 644
AasI GACNNNNNNGTC 1 cut(s) 746
AatII GACGTC 1 cut(s) 482
AccB7I CCANNNNNTGG 1 cut(s) 1610
AccI GTMKAC 1 cut(s) 1065
AccII CGCG 1 cut(s) 1845
AciI CCGC 6 cut(s) 308, 625, 715, 980, 1403, 1845
AclWI GGATC 1 cut(s) 495
AcoI YGGCCR 3 cut(s) 309, 763, 909
AcsI RAATTY 3 cut(s) 397, 1175, 2047
AcuI CTGAAG 2 cut(s) 615, 1173
AcvI CACGTG 1 cut(s) 116
AcyI GRCGYC 1 cut(s) 479
AfaI GTAC 9 cut(s) 126, 171, 204, 575, 935, 1477, 1687, 2018, 2034
AfiI CCNNNNNNNGG 5 cut(s) 265, 799, 1084, 1424, 1610
AflIII ACRYGT 1 cut(s) 113
AhlI ACTAGT 1 cut(s) 172
AjiI CACGTC 1 cut(s) 799
AjnI CCWGG 4 cut(s) 163, 1330, 1387, 1422
AjuI GAANNNNNNNTTGG 2 cut(s) 534, 566
Alw21I GWGCWC 2 cut(s) 971, 1702
Alw26I GTCTC 7 cut(s) 456, 763, 851, 1189, 1314, 1774, 1787
Alw44I GTGCAC 1 cut(s) 1698
AlwI GGATC 1 cut(s) 495
AlwNI CAGNNNCTG 2 cut(s) 815, 1813
AoxI GGCC 8 cut(s) 309, 622, 763, 909, 1035, 1534, 1615, 1918
ApaLI GTGCAC 1 cut(s) 1698
ApeKI GCWGC 6 cut(s) 410, 1166, 1400, 1631, 1718, 2003
ApoI RAATTY 3 cut(s) 397, 1175, 2047
Asp700I GAANNNNTTC 2 cut(s) 159, 243
AspLEI GCGC 3 cut(s) 41, 1279, 1558
AspS9I GGNCC 3 cut(s) 739, 748, 1266
AsuC2I CCSGG 1 cut(s) 497
AsuHPI GGTGA 8 cut(s) 398, 511, 994, 1013, 1439, 1651, 1685, 1881
AsuII TTCGAA 1 cut(s) 836
AvaII GGWCC 3 cut(s) 739, 748, 1266
AxyI CCTNAGG 1 cut(s) 1080
BaeGI GKGCMC 1 cut(s) 1702
BalI TGGCCA 1 cut(s) 911
BanII GRGCYC 5 cut(s) 497, 971, 1112, 1323, 1501
BauI CACGAG 1 cut(s) 1706
BbrPI CACGTG 1 cut(s) 116
BbsI GAAGAC 1 cut(s) 32
Bbv12I GWGCWC 2 cut(s) 971, 1702
BbvI GCAGC 6 cut(s) 422, 1178, 1387, 1618, 1705, 2015
BccI CCATC 5 cut(s) 364, 524, 772, 1082, 1277
BceAI ACGGC 2 cut(s) 702, 1712
BcgI CGANNNNNNTGC 2 cut(s) 1252, 1286
BciT130I CCWGG 4 cut(s) 165, 1332, 1389, 1424
BclI TGATCA 1 cut(s) 1974
BcnI CCSGG 1 cut(s) 497
BcoDI GTCTC 7 cut(s) 456, 763, 851, 1189, 1314, 1774, 1787
BcuI ACTAGT 1 cut(s) 172
BfaI CTAG 7 cut(s) 173, 1091, 1290, 1595, 1607, 1925, 1961
BisI GCNGC 8 cut(s) 309, 411, 625, 1167, 1401, 1632, 1719, 2004
BlpI GCTNAGC 1 cut(s) 1599
BlsI GCNGC 8 cut(s) 310, 412, 626, 1168, 1402, 1633, 1720, 2005
BmcAI AGTACT 1 cut(s) 1687
Bme1390I CCNGG 5 cut(s) 165, 497, 1332, 1389, 1424
Bme18I GGWCC 3 cut(s) 739, 748, 1266
BmgBI CACGTC 1 cut(s) 799
BmgT120I GGNCC 3 cut(s) 739, 748, 1266
BmiI GGNNCC 3 cut(s) 494, 749, 804
BmrFI CCNGG 5 cut(s) 165, 497, 1332, 1389, 1424
BmrI ACTGGG 1 cut(s) 1551
BmsI GCATC 6 cut(s) 50, 468, 615, 827, 1201, 1891
BmuI ACTGGG 1 cut(s) 1551
BpiI GAAGAC 1 cut(s) 32
BplI GAGNNNNNCTC 2 cut(s) 410, 442
BpmI CTGGAG 3 cut(s) 882, 1353, 1632
Bpu10I CCTNAGC 5 cut(s) 278, 587, 788, 970, 1723
Bpu1102I GCTNAGC 1 cut(s) 1599
Bpu14I TTCGAA 1 cut(s) 836
BpuEI CTTGAG 2 cut(s) 412, 545
BpuMI CCSGG 1 cut(s) 497
Bsa29I ATCGAT 2 cut(s) 462, 519
BsaAI YACGTR 2 cut(s) 116, 394
BsaHI GRCGYC 1 cut(s) 479
BsaI GGTCTC 3 cut(s) 763, 1314, 1787
BsaJI CCNNGG 4 cut(s) 1258, 1423, 1452, 1678
Bsc4I CCNNNNNNNGG 5 cut(s) 265, 799, 1084, 1424, 1610
Bse118I RCCGGY 1 cut(s) 1916
Bse1I ACTGG 5 cut(s) 809, 899, 912, 1016, 1546
Bse21I CCTNAGG 1 cut(s) 1080
Bse3DI GCAATG 5 cut(s) 216, 351, 1066, 1720, 1954
BseBI CCWGG 4 cut(s) 165, 1332, 1389, 1424
BseCI ATCGAT 2 cut(s) 462, 519
BseDI CCNNGG 4 cut(s) 1258, 1423, 1452, 1678
BseGI GGATG 5 cut(s) 41, 1074, 1204, 1882, 1906
BseLI CCNNNNNNNGG 5 cut(s) 265, 799, 1084, 1424, 1610
BseMI GCAATG 5 cut(s) 216, 351, 1066, 1720, 1954
BseMII CTCAG 7 cut(s) 222, 576, 633, 650, 984, 1094, 1998
BseNI ACTGG 5 cut(s) 809, 899, 912, 1016, 1546
BseSI GKGCMC 1 cut(s) 1702
BseXI GCAGC 6 cut(s) 422, 1178, 1387, 1618, 1705, 2015
BseYI CCCAGC 2 cut(s) 1493, 1813
BsgI GTGCAG 2 cut(s) 197, 2022
Bsh1236I CGCG 1 cut(s) 1845
BshFI GGCC 8 cut(s) 311, 624, 765, 911, 1037, 1536, 1617, 1920
BshVI ATCGAT 2 cut(s) 462, 519
BsiHKAI GWGCWC 2 cut(s) 971, 1702
BsiSI CCGG 3 cut(s) 497, 766, 1917
BslFI GGGAC 2 cut(s) 895, 1865
BslI CCNNNNNNNGG 5 cut(s) 265, 799, 1084, 1424, 1610
BsmAI GTCTC 7 cut(s) 456, 763, 851, 1189, 1314, 1774, 1787
BsmFI GGGAC 2 cut(s) 895, 1865
BsmI GAATGC 1 cut(s) 991
BsnI GGCC 8 cut(s) 311, 624, 765, 911, 1037, 1536, 1617, 1920
Bso31I GGTCTC 3 cut(s) 763, 1314, 1787
Bsp119I TTCGAA 1 cut(s) 836
Bsp1286I GDGCHC 6 cut(s) 497, 971, 1112, 1323, 1501, 1702
Bsp143I GATC 6 cut(s) 463, 487, 520, 647, 1974, 1996
Bsp1720I GCTNAGC 1 cut(s) 1599
Bsp19I CCATGG 2 cut(s) 1258, 1678
BspACI CCGC 6 cut(s) 308, 625, 715, 980, 1403, 1845
BspANI GGCC 8 cut(s) 311, 624, 765, 911, 1037, 1536, 1617, 1920
BspCNI CTCAG 7 cut(s) 223, 575, 632, 649, 983, 1093, 1999
BspDI ATCGAT 2 cut(s) 462, 519
BspFNI CGCG 1 cut(s) 1845
BspLI GGNNCC 3 cut(s) 494, 749, 804
BspPI GGATC 1 cut(s) 495
BspQI GCTCTTC 2 cut(s) 833, 1506
BspT104I TTCGAA 1 cut(s) 836
BspTNI GGTCTC 3 cut(s) 763, 1314, 1787
BsrDI GCAATG 5 cut(s) 216, 351, 1066, 1720, 1954
BsrFI RCCGGY 1 cut(s) 1916
BsrI ACTGG 5 cut(s) 809, 899, 912, 1016, 1546
BssAI RCCGGY 1 cut(s) 1916
BssECI CCNNGG 4 cut(s) 1258, 1423, 1452, 1678
BssMI GATC 6 cut(s) 463, 487, 520, 647, 1974, 1996
BssNI GRCGYC 1 cut(s) 479
BssSI CACGAG 1 cut(s) 1706
BssT1I CCWWGG 3 cut(s) 1258, 1452, 1678
Bst2BI CACGAG 1 cut(s) 1706
Bst2UI CCWGG 4 cut(s) 165, 1332, 1389, 1424
Bst4CI ACNGT 3 cut(s) 1127, 1445, 1766
Bst6I CTCTTC 3 cut(s) 537, 833, 1506
BstACI GRCGYC 1 cut(s) 479
BstAPI GCANNNNNTGC 1 cut(s) 1471
BstBAI YACGTR 2 cut(s) 116, 394
BstBI TTCGAA 1 cut(s) 836
BstC8I GCNNGC 3 cut(s) 1224, 1281, 1918
BstDSI CCRYGG 2 cut(s) 1258, 1678
BstEII GGTNACC 1 cut(s) 1673
BstF5I GGATG 5 cut(s) 41, 1074, 1204, 1882, 1906
BstFNI CGCG 1 cut(s) 1845
BstHHI GCGC 3 cut(s) 41, 1279, 1558
BstKTI GATC 6 cut(s) 466, 490, 523, 650, 1977, 1999
BstMAI GTCTC 7 cut(s) 456, 763, 851, 1189, 1314, 1774, 1787
BstMBI GATC 6 cut(s) 463, 487, 520, 647, 1974, 1996
BstMWI GCNNNNNNNGC 7 cut(s) 986, 1163, 1260, 1327, 1471, 1562, 1724
BstNI CCWGG 4 cut(s) 165, 1332, 1389, 1424
BstNSI RCATGY 1 cut(s) 1908
BstPI GGTNACC 1 cut(s) 1673
BstSCI CCNGG 5 cut(s) 163, 495, 1330, 1387, 1422
BstSLI GKGCMC 1 cut(s) 1702
BstSNI TACGTA 1 cut(s) 394
BstUI CGCG 1 cut(s) 1845
BstV1I GCAGC 6 cut(s) 422, 1178, 1387, 1618, 1705, 2015
BstV2I GAAGAC 1 cut(s) 32
BstXI CCANNNNNNTGG 1 cut(s) 1759
Bsu15I ATCGAT 2 cut(s) 462, 519
Bsu36I CCTNAGG 1 cut(s) 1080
BsuRI GGCC 8 cut(s) 311, 624, 765, 911, 1037, 1536, 1617, 1920
BsuTUI ATCGAT 2 cut(s) 462, 519
BtgI CCRYGG 2 cut(s) 1258, 1678
BtrI CACGTC 1 cut(s) 799
BtsCI GGATG 5 cut(s) 41, 1074, 1204, 1882, 1906
BtsIMutI CAGTG 5 cut(s) 906, 919, 1132, 1450, 1771
Cac8I GCNNGC 3 cut(s) 1224, 1281, 1918
CaiI CAGNNNCTG 2 cut(s) 815, 1813
CfoI GCGC 3 cut(s) 41, 1279, 1558
Cfr10I RCCGGY 1 cut(s) 1916
Cfr13I GGNCC 3 cut(s) 739, 748, 1266
ClaI ATCGAT 2 cut(s) 462, 519
Csp6I GTAC 9 cut(s) 125, 170, 203, 574, 934, 1476, 1686, 2017, 2033
CviQI GTAC 9 cut(s) 125, 170, 203, 574, 934, 1476, 1686, 2017, 2033
DpnI GATC 6 cut(s) 465, 489, 522, 649, 1976, 1998
DpnII GATC 6 cut(s) 463, 487, 520, 647, 1974, 1996
DrdI GACNNNNNNGTC 1 cut(s) 746
DseDI GACNNNNNNGTC 1 cut(s) 746
EaeI YGGCCR 3 cut(s) 309, 763, 909
Eam1104I CTCTTC 3 cut(s) 537, 833, 1506
EarI CTCTTC 3 cut(s) 537, 833, 1506
EciI GGCGGA 1 cut(s) 704
Ecl136II GAGCTC 1 cut(s) 969
Eco105I TACGTA 1 cut(s) 394
Eco130I CCWWGG 3 cut(s) 1258, 1452, 1678
Eco147I AGGCCT 1 cut(s) 1617
Eco24I GRGCYC 5 cut(s) 497, 971, 1112, 1323, 1501
Eco31I GGTCTC 3 cut(s) 763, 1314, 1787
Eco32I GATATC 1 cut(s) 1743
Eco47I GGWCC 3 cut(s) 739, 748, 1266
Eco53kI GAGCTC 1 cut(s) 969
Eco57I CTGAAG 2 cut(s) 615, 1173
Eco72I CACGTG 1 cut(s) 116
Eco81I CCTNAGG 1 cut(s) 1080
Eco91I GGTNACC 1 cut(s) 1673
EcoICRI GAGCTC 1 cut(s) 969
EcoO65I GGTNACC 1 cut(s) 1673
EcoRII CCWGG 4 cut(s) 163, 1330, 1387, 1422
EcoRV GATATC 1 cut(s) 1743
EcoT14I CCWWGG 3 cut(s) 1258, 1452, 1678
EcoT22I ATGCAT 1 cut(s) 1906
EcoT38I GRGCYC 5 cut(s) 497, 971, 1112, 1323, 1501
ErhI CCWWGG 3 cut(s) 1258, 1452, 1678
FalI AAGNNNNNCTT 4 cut(s) 261, 293, 1639, 1671
FaqI GGGAC 2 cut(s) 895, 1865
FbaI TGATCA 1 cut(s) 1974
FblI GTMKAC 1 cut(s) 1065
Fnu4HI GCNGC 8 cut(s) 309, 411, 625, 1167, 1401, 1632, 1719, 2004
FokI GGATG 5 cut(s) 28, 1061, 1191, 1889, 1913
FriOI GRGCYC 5 cut(s) 497, 971, 1112, 1323, 1501
Fsp4HI GCNGC 8 cut(s) 309, 411, 625, 1167, 1401, 1632, 1719, 2004
FspBI CTAG 7 cut(s) 173, 1091, 1290, 1595, 1607, 1925, 1961
GlaI GCGC 3 cut(s) 40, 1278, 1557
GluI GCNGC 8 cut(s) 309, 411, 625, 1167, 1401, 1632, 1719, 2004
GsaI CCCAGC 2 cut(s) 1497, 1817
GsuI CTGGAG 3 cut(s) 882, 1353, 1632
HaeIII GGCC 8 cut(s) 311, 624, 765, 911, 1037, 1536, 1617, 1920
HapII CCGG 3 cut(s) 497, 766, 1917
HhaI GCGC 3 cut(s) 41, 1279, 1558
Hin1I GRCGYC 1 cut(s) 479
Hin6I GCGC 3 cut(s) 39, 1277, 1556
HinP1I GCGC 3 cut(s) 39, 1277, 1556
HincII GTYRAC 1 cut(s) 1932
HindII GTYRAC 1 cut(s) 1932
HindIII AAGCTT 1 cut(s) 275
HinfI GANTC 6 cut(s) 144, 244, 440, 630, 1335, 1381
HpaI GTTAAC 1 cut(s) 1932
HpaII CCGG 3 cut(s) 497, 766, 1917
HphI GGTGA 8 cut(s) 398, 511, 994, 1013, 1439, 1651, 1685, 1881
Hpy166II GTNNAC 4 cut(s) 170, 1066, 1700, 1932
Hpy188I TCNGA 8 cut(s) 149, 249, 492, 639, 652, 752, 1194, 1809
Hpy188III TCNNGA 6 cut(s) 53, 429, 524, 1146, 1208, 1760
Hpy8I GTNNAC 4 cut(s) 170, 1066, 1700, 1932
Hpy99I CGWCG 1 cut(s) 1517
HpyAV CCTTC 6 cut(s) 244, 683, 862, 1012, 1424, 1931
HpyCH4III ACNGT 3 cut(s) 1127, 1445, 1766
HpyCH4IV ACGT 4 cut(s) 115, 393, 479, 798
HpyF10VI GCNNNNNNNGC 7 cut(s) 986, 1163, 1260, 1327, 1471, 1562, 1724
HpySE526I ACGT 4 cut(s) 115, 393, 479, 798
Hsp92I GRCGYC 1 cut(s) 479
HspAI GCGC 3 cut(s) 39, 1277, 1556
KroI GCCGGC 1 cut(s) 1916
KroNI GCCGGC 1 cut(s) 1918
Ksp22I TGATCA 1 cut(s) 1974
KspAI GTTAAC 1 cut(s) 1932
Kzo9I GATC 6 cut(s) 463, 487, 520, 647, 1974, 1996
LguI GCTCTTC 2 cut(s) 833, 1506
LmnI GCTCC 3 cut(s) 492, 1628, 1990
Lsp1109I GCAGC 6 cut(s) 422, 1178, 1387, 1618, 1705, 2015
LweI GCATC 6 cut(s) 50, 468, 615, 827, 1201, 1891
MaeI CTAG 7 cut(s) 173, 1091, 1290, 1595, 1607, 1925, 1961
MaeII ACGT 4 cut(s) 115, 393, 479, 798
MaeIII GTNAC 5 cut(s) 375, 811, 1336, 1639, 1673
MalI GATC 6 cut(s) 465, 489, 522, 649, 1976, 1998
MboI GATC 6 cut(s) 463, 487, 520, 647, 1974, 1996
MboII GAAGA 9 cut(s) 37, 281, 554, 601, 608, 850, 1126, 1376, 1493
MhlI GDGCHC 6 cut(s) 497, 971, 1112, 1323, 1501, 1702
MlsI TGGCCA 1 cut(s) 911
MluNI TGGCCA 1 cut(s) 911
MlyI GAGTC 2 cut(s) 153, 1344
MmeI TCCRAC 1 cut(s) 775
Mox20I TGGCCA 1 cut(s) 911
Mph1103I ATGCAT 1 cut(s) 1906
MroNI GCCGGC 1 cut(s) 1916
MroXI GAANNNNTTC 2 cut(s) 159, 243
MscI TGGCCA 1 cut(s) 911
MseI TTAA 4 cut(s) 63, 1173, 1802, 1931
MslI CAYNNNNRTG 2 cut(s) 1002, 1209
Msp20I TGGCCA 1 cut(s) 911
MspA1I CMGCKG 1 cut(s) 2006
MspI CCGG 3 cut(s) 497, 766, 1917
MspR9I CCNGG 5 cut(s) 165, 497, 1332, 1389, 1424
Mva1269I GAATGC 1 cut(s) 991
MvaI CCWGG 4 cut(s) 165, 1332, 1389, 1424
MvnI CGCG 1 cut(s) 1845
MwoI GCNNNNNNNGC 7 cut(s) 986, 1163, 1260, 1327, 1471, 1562, 1724
NaeI GCCGGC 1 cut(s) 1918
NciI CCSGG 1 cut(s) 497
NcoI CCATGG 2 cut(s) 1258, 1678
NdeII GATC 6 cut(s) 463, 487, 520, 647, 1974, 1996
NgoMIV GCCGGC 1 cut(s) 1916
NlaIV GGNNCC 3 cut(s) 494, 749, 804
NmuCI GTSAC 3 cut(s) 1336, 1639, 1673
NsiI ATGCAT 1 cut(s) 1906
NspI RCATGY 1 cut(s) 1908
NspV TTCGAA 1 cut(s) 836
PceI AGGCCT 1 cut(s) 1617
PciSI GCTCTTC 2 cut(s) 833, 1506
PctI GAATGC 1 cut(s) 991
PdiI GCCGGC 1 cut(s) 1918
PdmI GAANNNNTTC 2 cut(s) 159, 243
PfeI GAWTC 4 cut(s) 244, 440, 630, 1381
PflFI GACNNNGTC 1 cut(s) 1766
PflMI CCANNNNNTGG 1 cut(s) 1610
PkrI GCNGC 8 cut(s) 310, 412, 626, 1168, 1402, 1633, 1720, 2005
PleI GAGTC 2 cut(s) 152, 1343
PmaCI CACGTG 1 cut(s) 116
PmlI CACGTG 1 cut(s) 116
PpsI GAGTC 2 cut(s) 152, 1343
Ppu21I YACGTR 2 cut(s) 116, 394
PsiI TTATAA 1 cut(s) 644
Psp124BI GAGCTC 1 cut(s) 971
Psp6I CCWGG 4 cut(s) 163, 1330, 1387, 1422
PspCI CACGTG 1 cut(s) 116
PspEI GGTNACC 1 cut(s) 1673
PspFI CCCAGC 2 cut(s) 1493, 1813
PspGI CCWGG 4 cut(s) 163, 1330, 1387, 1422
PspN4I GGNNCC 3 cut(s) 494, 749, 804
PspPI GGNCC 3 cut(s) 739, 748, 1266
PstNI CAGNNNCTG 2 cut(s) 815, 1813
PsyI GACNNNGTC 1 cut(s) 1766
PvuII CAGCTG 1 cut(s) 2006
RsaI GTAC 9 cut(s) 126, 171, 204, 575, 935, 1477, 1687, 2018, 2034
RsaNI GTAC 9 cut(s) 125, 170, 203, 574, 934, 1476, 1686, 2017, 2033
RseI CAYNNNNRTG 2 cut(s) 1002, 1209
SacI GAGCTC 1 cut(s) 971
SapI GCTCTTC 2 cut(s) 833, 1506
SaqAI TTAA 4 cut(s) 63, 1173, 1802, 1931
SatI GCNGC 8 cut(s) 309, 411, 625, 1167, 1401, 1632, 1719, 2004
Sau3AI GATC 6 cut(s) 463, 487, 520, 647, 1974, 1996
Sau96I GGNCC 3 cut(s) 739, 748, 1266
ScaI AGTACT 1 cut(s) 1687
SchI GAGTC 2 cut(s) 153, 1344
ScrFI CCNGG 5 cut(s) 165, 497, 1332, 1389, 1424
SduI GDGCHC 6 cut(s) 497, 971, 1112, 1323, 1501, 1702
SfaNI GCATC 6 cut(s) 50, 468, 615, 827, 1201, 1891
SfuI TTCGAA 1 cut(s) 836
SinI GGWCC 3 cut(s) 739, 748, 1266
SmiMI CAYNNNNRTG 2 cut(s) 1002, 1209
SmlI CTYRAG 2 cut(s) 427, 524
SmoI CTYRAG 2 cut(s) 427, 524
SnaBI TACGTA 1 cut(s) 394
SpeI ACTAGT 1 cut(s) 172
SseBI AGGCCT 1 cut(s) 1617
SsiI CCGC 6 cut(s) 308, 625, 715, 980, 1403, 1845
SspMI CTAG 7 cut(s) 173, 1091, 1290, 1595, 1607, 1925, 1961
SstI GAGCTC 1 cut(s) 971
StuI AGGCCT 1 cut(s) 1617
StyD4I CCNGG 5 cut(s) 163, 495, 1330, 1387, 1422
StyI CCWWGG 3 cut(s) 1258, 1452, 1678
TaaI ACNGT 3 cut(s) 1127, 1445, 1766
TaiI ACGT 4 cut(s) 118, 396, 482, 801
TaqI TCGA 5 cut(s) 462, 519, 836, 942, 1512
TatI WGTACW 3 cut(s) 169, 1685, 2032
TauI GCSGC 2 cut(s) 311, 627
TfiI GAWTC 4 cut(s) 244, 440, 630, 1381
Tru1I TTAA 4 cut(s) 63, 1173, 1802, 1931
Tru9I TTAA 4 cut(s) 63, 1173, 1802, 1931
TscAI CASTG 5 cut(s) 906, 919, 1132, 1450, 1771
TseFI GTSAC 3 cut(s) 1336, 1639, 1673
TseI GCWGC 6 cut(s) 410, 1166, 1400, 1631, 1718, 2003
Tsp45I GTSAC 3 cut(s) 1336, 1639, 1673
TspDTI ATGAA 9 cut(s) 17, 38, 96, 182, 305, 362, 555, 601, 1225
TspGWI ACGGA 1 cut(s) 1302
TspRI CASTG 5 cut(s) 906, 919, 1132, 1450, 1771
Tth111I GACNNNGTC 1 cut(s) 1766
Van91I CCANNNNNTGG 1 cut(s) 1610
VneI GTGCAC 1 cut(s) 1698
VpaK11BI GGWCC 3 cut(s) 739, 748, 1266
XapI RAATTY 3 cut(s) 397, 1175, 2047
XceI RCATGY 1 cut(s) 1908
XcmI CCANNNNNNNNNTGG 1 cut(s) 1081
XmiI GTMKAC 1 cut(s) 1065
XmnI GAANNNNTTC 2 cut(s) 159, 243
XspI CTAG 7 cut(s) 173, 1091, 1290, 1595, 1607, 1925, 1961
ZraI GACGTC 1 cut(s) 480
ZrmI AGTACT 1 cut(s) 1687
Zsp2I ATGCAT 1 cut(s) 1906
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.