Transposase family tnp2
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 2 cut(s) | 28, 304 |
| AclWI | GGATC | 2 cut(s) | 1081, 1094 |
| AcoI | YGGCCR | 1 cut(s) | 722 |
| AcsI | RAATTY | 3 cut(s) | 255, 751, 935 |
| AcuI | CTGAAG | 2 cut(s) | 1076, 1137 |
| AcyI | GRCGYC | 1 cut(s) | 811 |
| AfaI | GTAC | 4 cut(s) | 39, 188, 339, 631 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 912, 1096 |
| AgsI | TTSAA | 5 cut(s) | 181, 273, 596, 820, 1032 |
| AhlI | ACTAGT | 1 cut(s) | 1054 |
| AluBI | AGCT | 4 cut(s) | 770, 952, 1000, 1144 |
| AluI | AGCT | 4 cut(s) | 770, 952, 1000, 1144 |
| Alw21I | GWGCWC | 1 cut(s) | 742 |
| Alw44I | GTGCAC | 1 cut(s) | 738 |
| AlwI | GGATC | 2 cut(s) | 1081, 1094 |
| AoxI | GGCC | 4 cut(s) | 722, 866, 910, 963 |
| ApaI | GGGCCC | 1 cut(s) | 870 |
| ApaLI | GTGCAC | 1 cut(s) | 738 |
| ApeKI | GCWGC | 1 cut(s) | 770 |
| ApoI | RAATTY | 3 cut(s) | 255, 751, 935 |
| AspLEI | GCGC | 1 cut(s) | 715 |
| AspS9I | GGNCC | 5 cut(s) | 647, 866, 867, 910, 963 |
| AsuC2I | CCSGG | 1 cut(s) | 131 |
| AsuHPI | GGTGA | 3 cut(s) | 128, 865, 946 |
| AvaII | GGWCC | 1 cut(s) | 647 |
| BaeGI | GKGCMC | 2 cut(s) | 742, 870 |
| BalI | TGGCCA | 1 cut(s) | 724 |
| BamHI | GGATCC | 1 cut(s) | 1086 |
| BanII | GRGCYC | 2 cut(s) | 870, 1107 |
| Bbv12I | GWGCWC | 1 cut(s) | 742 |
| BbvI | GCAGC | 1 cut(s) | 757 |
| BccI | CCATC | 5 cut(s) | 49, 668, 770, 1026, 1121 |
| BceAI | ACGGC | 2 cut(s) | 366, 396 |
| BcnI | CCSGG | 1 cut(s) | 131 |
| BcuI | ACTAGT | 1 cut(s) | 1054 |
| BfaI | CTAG | 2 cut(s) | 929, 1055 |
| BfoI | RGCGCY | 1 cut(s) | 716 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 721 |
| BisI | GCNGC | 1 cut(s) | 771 |
| BlsI | GCNGC | 1 cut(s) | 772 |
| Bme1390I | CCNGG | 1 cut(s) | 131 |
| Bme18I | GGWCC | 1 cut(s) | 647 |
| BmgT120I | GGNCC | 5 cut(s) | 647, 866, 867, 910, 963 |
| BmiI | GGNNCC | 3 cut(s) | 868, 869, 1088 |
| BmrFI | CCNGG | 1 cut(s) | 131 |
| BmsI | GCATC | 2 cut(s) | 304, 998 |
| BpuEI | CTTGAG | 1 cut(s) | 573 |
| BpuMI | CCSGG | 1 cut(s) | 131 |
| Bsa29I | ATCGAT | 1 cut(s) | 205 |
| BsaHI | GRCGYC | 1 cut(s) | 811 |
| BsaJI | CCNNGG | 1 cut(s) | 906 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 912, 1096 |
| Bse118I | RCCGGY | 1 cut(s) | 912 |
| BseCI | ATCGAT | 1 cut(s) | 205 |
| BseDI | CCNNGG | 1 cut(s) | 906 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 912, 1096 |
| BseMII | CTCAG | 1 cut(s) | 39 |
| BseSI | GKGCMC | 2 cut(s) | 742, 870 |
| BseXI | GCAGC | 1 cut(s) | 757 |
| BshFI | GGCC | 4 cut(s) | 724, 868, 912, 965 |
| BshVI | ATCGAT | 1 cut(s) | 205 |
| BsiHKAI | GWGCWC | 1 cut(s) | 742 |
| BsiSI | CCGG | 2 cut(s) | 131, 913 |
| BslFI | GGGAC | 1 cut(s) | 713 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 912, 1096 |
| BsmFI | GGGAC | 1 cut(s) | 713 |
| BsmI | GAATGC | 1 cut(s) | 271 |
| BsnI | GGCC | 4 cut(s) | 724, 868, 912, 965 |
| Bsp120I | GGGCCC | 1 cut(s) | 866 |
| Bsp1286I | GDGCHC | 3 cut(s) | 742, 870, 1107 |
| Bsp1407I | TGTACA | 1 cut(s) | 629 |
| Bsp143I | GATC | 2 cut(s) | 298, 1086 |
| BspANI | GGCC | 4 cut(s) | 724, 868, 912, 965 |
| BspCNI | CTCAG | 1 cut(s) | 40 |
| BspDI | ATCGAT | 1 cut(s) | 205 |
| BspHI | TCATGA | 3 cut(s) | 73, 192, 918 |
| BspLI | GGNNCC | 3 cut(s) | 868, 869, 1088 |
| BspPI | GGATC | 2 cut(s) | 1081, 1094 |
| BspQI | GCTCTTC | 1 cut(s) | 1095 |
| BsrFI | RCCGGY | 1 cut(s) | 912 |
| BsrGI | TGTACA | 1 cut(s) | 629 |
| BssAI | RCCGGY | 1 cut(s) | 912 |
| BssECI | CCNNGG | 1 cut(s) | 906 |
| BssMI | GATC | 2 cut(s) | 298, 1086 |
| BssNI | GRCGYC | 1 cut(s) | 811 |
| Bst4CI | ACNGT | 4 cut(s) | 177, 251, 335, 877 |
| Bst6I | CTCTTC | 1 cut(s) | 1095 |
| BstACI | GRCGYC | 1 cut(s) | 811 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 986 |
| BstAUI | TGTACA | 1 cut(s) | 629 |
| BstC8I | GCNNGC | 2 cut(s) | 914, 1002 |
| BstDEI | CTNAG | 1 cut(s) | 48 |
| BstDSI | CCRYGG | 1 cut(s) | 906 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1094 |
| BstH2I | RGCGCY | 1 cut(s) | 716 |
| BstHHI | GCGC | 1 cut(s) | 715 |
| BstKTI | GATC | 2 cut(s) | 301, 1089 |
| BstMBI | GATC | 2 cut(s) | 298, 1086 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 721, 986 |
| BstSCI | CCNGG | 1 cut(s) | 129 |
| BstSLI | GKGCMC | 2 cut(s) | 742, 870 |
| BstV1I | GCAGC | 1 cut(s) | 757 |
| BstX2I | RGATCY | 1 cut(s) | 1086 |
| BstYI | RGATCY | 1 cut(s) | 1086 |
| Bsu15I | ATCGAT | 1 cut(s) | 205 |
| BsuRI | GGCC | 4 cut(s) | 724, 868, 912, 965 |
| BsuTUI | ATCGAT | 1 cut(s) | 205 |
| BtgI | CCRYGG | 1 cut(s) | 906 |
| BtsIMutI | CAGTG | 2 cut(s) | 228, 256 |
| Cac8I | GCNNGC | 2 cut(s) | 914, 1002 |
| CciI | TCATGA | 3 cut(s) | 73, 192, 918 |
| CfoI | GCGC | 1 cut(s) | 715 |
| Cfr10I | RCCGGY | 1 cut(s) | 912 |
| Cfr13I | GGNCC | 5 cut(s) | 647, 866, 867, 910, 963 |
| ClaI | ATCGAT | 1 cut(s) | 205 |
| CseI | GACGC | 1 cut(s) | 800 |
| Csp6I | GTAC | 4 cut(s) | 38, 187, 338, 630 |
| CviAII | CATG | 5 cut(s) | 74, 193, 319, 843, 919 |
| CviQI | GTAC | 4 cut(s) | 38, 187, 338, 630 |
| DdeI | CTNAG | 1 cut(s) | 48 |
| DpnI | GATC | 2 cut(s) | 300, 1088 |
| DpnII | GATC | 2 cut(s) | 298, 1086 |
| DraI | TTTAAA | 2 cut(s) | 85, 948 |
| EaeI | YGGCCR | 1 cut(s) | 722 |
| Eam1104I | CTCTTC | 1 cut(s) | 1095 |
| EarI | CTCTTC | 1 cut(s) | 1095 |
| Eco24I | GRGCYC | 2 cut(s) | 870, 1107 |
| Eco32I | GATATC | 2 cut(s) | 124, 203 |
| Eco47I | GGWCC | 1 cut(s) | 647 |
| Eco57I | CTGAAG | 2 cut(s) | 1076, 1137 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1094 |
| EcoO109I | RGGNCCY | 2 cut(s) | 866, 867 |
| EcoRI | GAATTC | 1 cut(s) | 935 |
| EcoRV | GATATC | 2 cut(s) | 124, 203 |
| EcoT38I | GRGCYC | 2 cut(s) | 870, 1107 |
| FaeI | CATG | 5 cut(s) | 77, 196, 322, 846, 922 |
| FaqI | GGGAC | 1 cut(s) | 713 |
| FatI | CATG | 5 cut(s) | 73, 192, 318, 842, 918 |
| FblI | GTMKAC | 2 cut(s) | 28, 304 |
| Fnu4HI | GCNGC | 1 cut(s) | 771 |
| FriOI | GRGCYC | 2 cut(s) | 870, 1107 |
| Fsp4HI | GCNGC | 1 cut(s) | 771 |
| FspBI | CTAG | 2 cut(s) | 929, 1055 |
| GlaI | GCGC | 1 cut(s) | 714 |
| GluI | GCNGC | 1 cut(s) | 771 |
| HaeII | RGCGCY | 1 cut(s) | 716 |
| HaeIII | GGCC | 4 cut(s) | 724, 868, 912, 965 |
| HapII | CCGG | 2 cut(s) | 131, 913 |
| HgaI | GACGC | 1 cut(s) | 800 |
| HhaI | GCGC | 1 cut(s) | 715 |
| Hin1I | GRCGYC | 1 cut(s) | 811 |
| Hin1II | CATG | 5 cut(s) | 77, 196, 322, 846, 922 |
| Hin6I | GCGC | 1 cut(s) | 713 |
| HinP1I | GCGC | 1 cut(s) | 713 |
| HincII | GTYRAC | 1 cut(s) | 305 |
| HindII | GTYRAC | 1 cut(s) | 305 |
| HinfI | GANTC | 7 cut(s) | 44, 61, 233, 491, 829, 896, 1025 |
| HpaII | CCGG | 2 cut(s) | 131, 913 |
| HphI | GGTGA | 3 cut(s) | 128, 865, 946 |
| Hpy166II | GTNNAC | 5 cut(s) | 29, 305, 338, 509, 740 |
| Hpy188I | TCNGA | 4 cut(s) | 49, 346, 977, 1111 |
| Hpy8I | GTNNAC | 5 cut(s) | 29, 305, 338, 509, 740 |
| Hpy99I | CGWCG | 1 cut(s) | 306 |
| HpyAV | CCTTC | 2 cut(s) | 885, 1100 |
| HpyCH4III | ACNGT | 4 cut(s) | 177, 251, 335, 877 |
| HpyCH4IV | ACGT | 2 cut(s) | 31, 213 |
| HpyCH4V | TGCA | 5 cut(s) | 143, 242, 441, 740, 989 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 721, 986 |
| HpyF3I | CTNAG | 1 cut(s) | 48 |
| HpySE526I | ACGT | 2 cut(s) | 31, 213 |
| Hsp92I | GRCGYC | 1 cut(s) | 811 |
| Hsp92II | CATG | 5 cut(s) | 77, 196, 322, 846, 922 |
| HspAI | GCGC | 1 cut(s) | 713 |
| KroI | GCCGGC | 1 cut(s) | 912 |
| KroNI | GCCGGC | 1 cut(s) | 914 |
| Kzo9I | GATC | 2 cut(s) | 298, 1086 |
| LguI | GCTCTTC | 1 cut(s) | 1095 |
| LmnI | GCTCC | 2 cut(s) | 710, 977 |
| LpnPI | CCDG | 9 cut(s) | 144, 480, 926, 946, 954, 986, 1007, 1079, 1134 |
| Lsp1109I | GCAGC | 1 cut(s) | 757 |
| LweI | GCATC | 2 cut(s) | 304, 998 |
| MaeI | CTAG | 2 cut(s) | 929, 1055 |
| MaeII | ACGT | 2 cut(s) | 31, 213 |
| MaeIII | GTNAC | 2 cut(s) | 219, 812 |
| MalI | GATC | 2 cut(s) | 300, 1088 |
| MboI | GATC | 2 cut(s) | 298, 1086 |
| MflI | RGATCY | 1 cut(s) | 1086 |
| MhlI | GDGCHC | 3 cut(s) | 742, 870, 1107 |
| MlsI | TGGCCA | 1 cut(s) | 724 |
| MluNI | TGGCCA | 1 cut(s) | 724 |
| MlyI | GAGTC | 1 cut(s) | 838 |
| MmeI | TCCRAC | 1 cut(s) | 481 |
| Mox20I | TGGCCA | 1 cut(s) | 724 |
| MroNI | GCCGGC | 1 cut(s) | 912 |
| MscI | TGGCCA | 1 cut(s) | 724 |
| MseI | TTAA | 5 cut(s) | 84, 216, 693, 947, 1010 |
| Msp20I | TGGCCA | 1 cut(s) | 724 |
| MspI | CCGG | 2 cut(s) | 131, 913 |
| MspR9I | CCNGG | 1 cut(s) | 131 |
| Mva1269I | GAATGC | 1 cut(s) | 271 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 721, 986 |
| NaeI | GCCGGC | 1 cut(s) | 914 |
| NciI | CCSGG | 1 cut(s) | 131 |
| NdeII | GATC | 2 cut(s) | 298, 1086 |
| NgoMIV | GCCGGC | 1 cut(s) | 912 |
| NlaIII | CATG | 5 cut(s) | 77, 196, 322, 846, 922 |
| NlaIV | GGNNCC | 3 cut(s) | 868, 869, 1088 |
| NmuCI | GTSAC | 2 cut(s) | 219, 812 |
| PagI | TCATGA | 3 cut(s) | 73, 192, 918 |
| PciSI | GCTCTTC | 1 cut(s) | 1095 |
| PctI | GAATGC | 1 cut(s) | 271 |
| PdiI | GCCGGC | 1 cut(s) | 914 |
| PfeI | GAWTC | 6 cut(s) | 44, 61, 233, 491, 896, 1025 |
| PkrI | GCNGC | 1 cut(s) | 772 |
| PleI | GAGTC | 1 cut(s) | 837 |
| PpsI | GAGTC | 1 cut(s) | 837 |
| PspN4I | GGNNCC | 3 cut(s) | 868, 869, 1088 |
| PspOMI | GGGCCC | 1 cut(s) | 866 |
| PspPI | GGNCC | 5 cut(s) | 647, 866, 867, 910, 963 |
| PsuI | RGATCY | 1 cut(s) | 1086 |
| RsaI | GTAC | 4 cut(s) | 39, 188, 339, 631 |
| RsaNI | GTAC | 4 cut(s) | 38, 187, 338, 630 |
| SalI | GTCGAC | 1 cut(s) | 303 |
| SapI | GCTCTTC | 1 cut(s) | 1095 |
| SaqAI | TTAA | 5 cut(s) | 84, 216, 693, 947, 1010 |
| SatI | GCNGC | 1 cut(s) | 771 |
| Sau3AI | GATC | 2 cut(s) | 298, 1086 |
| Sau96I | GGNCC | 5 cut(s) | 647, 866, 867, 910, 963 |
| SchI | GAGTC | 1 cut(s) | 838 |
| ScrFI | CCNGG | 1 cut(s) | 131 |
| SduI | GDGCHC | 3 cut(s) | 742, 870, 1107 |
| SfaNI | GCATC | 2 cut(s) | 304, 998 |
| SinI | GGWCC | 1 cut(s) | 647 |
| SmlI | CTYRAG | 1 cut(s) | 588 |
| SmoI | CTYRAG | 1 cut(s) | 588 |
| SpeI | ACTAGT | 1 cut(s) | 1054 |
| SspI | AATATT | 2 cut(s) | 655, 799 |
| SspMI | CTAG | 2 cut(s) | 929, 1055 |
| StyD4I | CCNGG | 1 cut(s) | 129 |
| TaaI | ACNGT | 4 cut(s) | 177, 251, 335, 877 |
| TaiI | ACGT | 2 cut(s) | 34, 216 |
| TaqI | TCGA | 7 cut(s) | 9, 126, 205, 304, 324, 992, 1039 |
| TatI | WGTACW | 4 cut(s) | 37, 186, 337, 629 |
| TfiI | GAWTC | 6 cut(s) | 44, 61, 233, 491, 896, 1025 |
| Tru1I | TTAA | 5 cut(s) | 84, 216, 693, 947, 1010 |
| Tru9I | TTAA | 5 cut(s) | 84, 216, 693, 947, 1010 |
| TscAI | CASTG | 2 cut(s) | 235, 256 |
| TseFI | GTSAC | 2 cut(s) | 219, 812 |
| TseI | GCWGC | 1 cut(s) | 770 |
| Tsp45I | GTSAC | 2 cut(s) | 219, 812 |
| TspDTI | ATGAA | 5 cut(s) | 62, 209, 618, 650, 996 |
| TspRI | CASTG | 2 cut(s) | 235, 256 |
| VneI | GTGCAC | 1 cut(s) | 738 |
| VpaK11BI | GGWCC | 1 cut(s) | 647 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1094 |
| XapI | RAATTY | 3 cut(s) | 255, 751, 935 |
| XmiI | GTMKAC | 2 cut(s) | 28, 304 |
| XspI | CTAG | 2 cut(s) | 929, 1055 |