Transposase family tnp2
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 4 cut(s) | 87, 365, 689, 730 |
| AclWI | GGATC | 1 cut(s) | 416 |
| AcoI | YGGCCR | 1 cut(s) | 481 |
| AcuI | CTGAAG | 1 cut(s) | 83 |
| AfaI | GTAC | 1 cut(s) | 633 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 760 |
| AflIII | ACRYGT | 1 cut(s) | 770 |
| AgeI | ACCGGT | 1 cut(s) | 634 |
| AgsI | TTSAA | 1 cut(s) | 245 |
| AjnI | CCWGG | 1 cut(s) | 697 |
| AluBI | AGCT | 4 cut(s) | 55, 77, 97, 336 |
| AluI | AGCT | 4 cut(s) | 55, 77, 97, 336 |
| Alw21I | GWGCWC | 1 cut(s) | 661 |
| Alw26I | GTCTC | 3 cut(s) | 243, 296, 645 |
| Alw44I | GTGCAC | 1 cut(s) | 657 |
| AlwI | GGATC | 1 cut(s) | 416 |
| AoxI | GGCC | 1 cut(s) | 481 |
| ApaLI | GTGCAC | 1 cut(s) | 657 |
| ApeKI | GCWGC | 2 cut(s) | 361, 533 |
| AsiGI | ACCGGT | 1 cut(s) | 634 |
| AsuHPI | GGTGA | 1 cut(s) | 191 |
| BaeGI | GKGCMC | 1 cut(s) | 661 |
| Bbv12I | GWGCWC | 1 cut(s) | 661 |
| BbvI | GCAGC | 2 cut(s) | 348, 545 |
| BccI | CCATC | 4 cut(s) | 170, 279, 439, 762 |
| BceAI | ACGGC | 2 cut(s) | 336, 468 |
| BciT130I | CCWGG | 1 cut(s) | 699 |
| BciVI | GTATCC | 1 cut(s) | 717 |
| BcoDI | GTCTC | 3 cut(s) | 243, 296, 645 |
| BfaI | CTAG | 1 cut(s) | 78 |
| BfmI | CTRYAG | 2 cut(s) | 117, 513 |
| BfuI | GTATCC | 1 cut(s) | 717 |
| BisI | GCNGC | 6 cut(s) | 87, 362, 365, 534, 690, 731 |
| BlsI | GCNGC | 6 cut(s) | 88, 363, 366, 535, 691, 732 |
| Bme1390I | CCNGG | 1 cut(s) | 699 |
| BmiI | GGNNCC | 2 cut(s) | 6, 213 |
| BmrFI | CCNGG | 1 cut(s) | 699 |
| BmsI | GCATC | 1 cut(s) | 426 |
| Bsa29I | ATCGAT | 1 cut(s) | 434 |
| BsaBI | GATNNNNATC | 1 cut(s) | 420 |
| BsaJI | CCNNGG | 3 cut(s) | 8, 644, 697 |
| BsaWI | WCCGGW | 1 cut(s) | 634 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 621, 651 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 760 |
| Bse118I | RCCGGY | 1 cut(s) | 634 |
| Bse1I | ACTGG | 1 cut(s) | 409 |
| Bse8I | GATNNNNATC | 1 cut(s) | 420 |
| BseBI | CCWGG | 1 cut(s) | 699 |
| BseCI | ATCGAT | 1 cut(s) | 434 |
| BseDI | CCNNGG | 3 cut(s) | 8, 644, 697 |
| BseGI | GGATG | 3 cut(s) | 178, 290, 421 |
| BseJI | GATNNNNATC | 1 cut(s) | 420 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 760 |
| BseNI | ACTGG | 1 cut(s) | 409 |
| BseSI | GKGCMC | 1 cut(s) | 661 |
| BseXI | GCAGC | 2 cut(s) | 348, 545 |
| BshFI | GGCC | 1 cut(s) | 483 |
| BshTI | ACCGGT | 1 cut(s) | 634 |
| BshVI | ATCGAT | 1 cut(s) | 434 |
| BsiHKAI | GWGCWC | 1 cut(s) | 661 |
| BsiSI | CCGG | 1 cut(s) | 635 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 760 |
| BsmAI | GTCTC | 3 cut(s) | 243, 296, 645 |
| BsnI | GGCC | 1 cut(s) | 483 |
| Bsp1286I | GDGCHC | 1 cut(s) | 661 |
| Bsp143I | GATC | 1 cut(s) | 421 |
| Bsp19I | CCATGG | 1 cut(s) | 8 |
| BspACI | CCGC | 4 cut(s) | 87, 365, 689, 730 |
| BspANI | GGCC | 1 cut(s) | 483 |
| BspDI | ATCGAT | 1 cut(s) | 434 |
| BspLI | GGNNCC | 2 cut(s) | 6, 213 |
| BspPI | GGATC | 1 cut(s) | 416 |
| BsrFI | RCCGGY | 1 cut(s) | 634 |
| BsrI | ACTGG | 1 cut(s) | 409 |
| BssAI | RCCGGY | 1 cut(s) | 634 |
| BssECI | CCNNGG | 3 cut(s) | 8, 644, 697 |
| BssMI | GATC | 1 cut(s) | 421 |
| BssT1I | CCWWGG | 2 cut(s) | 8, 644 |
| Bst2UI | CCWGG | 1 cut(s) | 699 |
| Bst4CI | ACNGT | 4 cut(s) | 125, 234, 574, 663 |
| Bst6I | CTCTTC | 2 cut(s) | 17, 246 |
| BstDSI | CCRYGG | 1 cut(s) | 8 |
| BstF5I | GGATG | 3 cut(s) | 178, 290, 421 |
| BstKTI | GATC | 1 cut(s) | 424 |
| BstMAI | GTCTC | 3 cut(s) | 243, 296, 645 |
| BstMBI | GATC | 1 cut(s) | 421 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 61, 83, 598 |
| BstNI | CCWGG | 1 cut(s) | 699 |
| BstNSI | RCATGY | 1 cut(s) | 774 |
| BstSCI | CCNGG | 1 cut(s) | 697 |
| BstSFI | CTRYAG | 2 cut(s) | 117, 513 |
| BstSLI | GKGCMC | 1 cut(s) | 661 |
| BstV1I | GCAGC | 2 cut(s) | 348, 545 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 479 |
| Bsu15I | ATCGAT | 1 cut(s) | 434 |
| BsuI | GTATCC | 1 cut(s) | 717 |
| BsuRI | GGCC | 1 cut(s) | 483 |
| BsuTUI | ATCGAT | 1 cut(s) | 434 |
| BtgI | CCRYGG | 1 cut(s) | 8 |
| BtsCI | GGATG | 3 cut(s) | 178, 290, 421 |
| BtsIMutI | CAGTG | 3 cut(s) | 130, 299, 378 |
| Cfr10I | RCCGGY | 1 cut(s) | 634 |
| ClaI | ATCGAT | 1 cut(s) | 434 |
| Csp6I | GTAC | 1 cut(s) | 632 |
| CspAI | ACCGGT | 1 cut(s) | 634 |
| CviAII | CATG | 4 cut(s) | 9, 400, 440, 771 |
| CviQI | GTAC | 1 cut(s) | 632 |
| DpnI | GATC | 1 cut(s) | 423 |
| DpnII | GATC | 1 cut(s) | 421 |
| EaeI | YGGCCR | 1 cut(s) | 481 |
| Eam1104I | CTCTTC | 2 cut(s) | 17, 246 |
| EarI | CTCTTC | 2 cut(s) | 17, 246 |
| Eco130I | CCWWGG | 2 cut(s) | 8, 644 |
| Eco57I | CTGAAG | 1 cut(s) | 83 |
| EcoRII | CCWGG | 1 cut(s) | 697 |
| EcoT14I | CCWWGG | 2 cut(s) | 8, 644 |
| EcoT22I | ATGCAT | 1 cut(s) | 441 |
| ErhI | CCWWGG | 2 cut(s) | 8, 644 |
| FaeI | CATG | 4 cut(s) | 12, 403, 443, 774 |
| FatI | CATG | 4 cut(s) | 8, 399, 439, 770 |
| FauNDI | CATATG | 2 cut(s) | 217, 325 |
| Fnu4HI | GCNGC | 6 cut(s) | 87, 362, 365, 534, 690, 731 |
| FokI | GGATG | 3 cut(s) | 185, 297, 428 |
| Fsp4HI | GCNGC | 6 cut(s) | 87, 362, 365, 534, 690, 731 |
| FspBI | CTAG | 1 cut(s) | 78 |
| GluI | GCNGC | 6 cut(s) | 87, 362, 365, 534, 690, 731 |
| HaeIII | GGCC | 1 cut(s) | 483 |
| HapII | CCGG | 1 cut(s) | 635 |
| Hin1II | CATG | 4 cut(s) | 12, 403, 443, 774 |
| HindIII | AAGCTT | 1 cut(s) | 95 |
| HpaII | CCGG | 1 cut(s) | 635 |
| HphI | GGTGA | 1 cut(s) | 191 |
| Hpy166II | GTNNAC | 1 cut(s) | 659 |
| Hpy188I | TCNGA | 1 cut(s) | 372 |
| Hpy188III | TCNNGA | 1 cut(s) | 101 |
| Hpy8I | GTNNAC | 1 cut(s) | 659 |
| HpyAV | CCTTC | 3 cut(s) | 21, 127, 274 |
| HpyCH4III | ACNGT | 4 cut(s) | 125, 234, 574, 663 |
| HpyCH4V | TGCA | 3 cut(s) | 439, 533, 659 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 61, 83, 598 |
| Hsp92II | CATG | 4 cut(s) | 12, 403, 443, 774 |
| Kzo9I | GATC | 1 cut(s) | 421 |
| LmnI | GCTCC | 3 cut(s) | 4, 217, 341 |
| LpnPI | CCDG | 7 cut(s) | 86, 103, 219, 422, 648, 684, 711 |
| Lsp1109I | GCAGC | 2 cut(s) | 348, 545 |
| LweI | GCATC | 1 cut(s) | 426 |
| MaeI | CTAG | 1 cut(s) | 78 |
| MaeIII | GTNAC | 3 cut(s) | 179, 376, 545 |
| MalI | GATC | 1 cut(s) | 423 |
| MboI | GATC | 1 cut(s) | 421 |
| MboII | GAAGA | 6 cut(s) | 34, 44, 151, 154, 233, 238 |
| MhlI | GDGCHC | 1 cut(s) | 661 |
| MluCI | AATT | 4 cut(s) | 391, 452, 507, 576 |
| MmeI | TCCRAC | 2 cut(s) | 189, 393 |
| MnlI | CCTC | 7 cut(s) | 163, 202, 354, 378, 535, 557, 772 |
| Mph1103I | ATGCAT | 1 cut(s) | 441 |
| MseI | TTAA | 3 cut(s) | 57, 579, 612 |
| MslI | CAYNNNNRTG | 2 cut(s) | 383, 444 |
| MspI | CCGG | 1 cut(s) | 635 |
| MspR9I | CCNGG | 1 cut(s) | 699 |
| MvaI | CCWGG | 1 cut(s) | 699 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 61, 83, 598 |
| NcoI | CCATGG | 1 cut(s) | 8 |
| NdeI | CATATG | 2 cut(s) | 217, 325 |
| NdeII | GATC | 1 cut(s) | 421 |
| NlaIII | CATG | 4 cut(s) | 12, 403, 443, 774 |
| NlaIV | GGNNCC | 2 cut(s) | 6, 213 |
| NmuCI | GTSAC | 2 cut(s) | 179, 376 |
| NsiI | ATGCAT | 1 cut(s) | 441 |
| NspI | RCATGY | 1 cut(s) | 774 |
| PciI | ACATGT | 1 cut(s) | 770 |
| PinAI | ACCGGT | 1 cut(s) | 634 |
| PkrI | GCNGC | 6 cut(s) | 88, 363, 366, 535, 691, 732 |
| PscI | ACATGT | 1 cut(s) | 770 |
| Psp6I | CCWGG | 1 cut(s) | 697 |
| PspGI | CCWGG | 1 cut(s) | 697 |
| PspN4I | GGNNCC | 2 cut(s) | 6, 213 |
| RsaI | GTAC | 1 cut(s) | 633 |
| RsaNI | GTAC | 1 cut(s) | 632 |
| RseI | CAYNNNNRTG | 2 cut(s) | 383, 444 |
| SaqAI | TTAA | 3 cut(s) | 57, 579, 612 |
| SatI | GCNGC | 6 cut(s) | 87, 362, 365, 534, 690, 731 |
| Sau3AI | GATC | 1 cut(s) | 421 |
| ScrFI | CCNGG | 1 cut(s) | 699 |
| SduI | GDGCHC | 1 cut(s) | 661 |
| SetI | ASST | 6 cut(s) | 57, 79, 99, 338, 346, 783 |
| SfaNI | GCATC | 1 cut(s) | 426 |
| SfcI | CTRYAG | 2 cut(s) | 117, 513 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 383, 444 |
| Sse9I | AATT | 4 cut(s) | 391, 452, 507, 576 |
| SsiI | CCGC | 4 cut(s) | 87, 365, 689, 730 |
| SspMI | CTAG | 1 cut(s) | 78 |
| StyD4I | CCNGG | 1 cut(s) | 697 |
| StyI | CCWWGG | 2 cut(s) | 8, 644 |
| TaaI | ACNGT | 4 cut(s) | 125, 234, 574, 663 |
| TaqI | TCGA | 1 cut(s) | 434 |
| TasI | AATT | 4 cut(s) | 391, 452, 507, 576 |
| TauI | GCSGC | 4 cut(s) | 89, 367, 692, 733 |
| Tru1I | TTAA | 3 cut(s) | 57, 579, 612 |
| Tru9I | TTAA | 3 cut(s) | 57, 579, 612 |
| TscAI | CASTG | 3 cut(s) | 130, 299, 385 |
| TseFI | GTSAC | 2 cut(s) | 179, 376 |
| TseI | GCWGC | 2 cut(s) | 361, 533 |
| Tsp45I | GTSAC | 2 cut(s) | 179, 376 |
| TspDTI | ATGAA | 2 cut(s) | 456, 664 |
| TspGWI | ACGGA | 1 cut(s) | 255 |
| TspRI | CASTG | 3 cut(s) | 130, 299, 385 |
| VneI | GTGCAC | 1 cut(s) | 657 |
| XceI | RCATGY | 1 cut(s) | 774 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 397 |
| XspI | CTAG | 1 cut(s) | 78 |
| Zsp2I | ATGCAT | 1 cut(s) | 441 |