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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AluBI | AGCT | 1 cut(s) | 15 |
| AluI | AGCT | 1 cut(s) | 15 |
| AsuNHI | GCTAGC | 1 cut(s) | 28 |
| BceAI | ACGGC | 1 cut(s) | 72 |
| BfaI | CTAG | 1 cut(s) | 29 |
| BmsI | GCATC | 1 cut(s) | 15 |
| BmtI | GCTAGC | 1 cut(s) | 32 |
| BpmI | CTGGAG | 1 cut(s) | 192 |
| Bsa29I | ATCGAT | 1 cut(s) | 102 |
| BseCI | ATCGAT | 1 cut(s) | 102 |
| BshVI | ATCGAT | 1 cut(s) | 102 |
| Bsp143I | GATC | 1 cut(s) | 49 |
| BspDI | ATCGAT | 1 cut(s) | 102 |
| BspOI | GCTAGC | 1 cut(s) | 32 |
| BssMI | GATC | 1 cut(s) | 49 |
| BstC8I | GCNNGC | 1 cut(s) | 30 |
| BstKTI | GATC | 1 cut(s) | 52 |
| BstMBI | GATC | 1 cut(s) | 49 |
| Bsu15I | ATCGAT | 1 cut(s) | 102 |
| BsuTUI | ATCGAT | 1 cut(s) | 102 |
| Cac8I | GCNNGC | 1 cut(s) | 30 |
| ClaI | ATCGAT | 1 cut(s) | 102 |
| CviJI | RGCY | 3 cut(s) | 15, 32, 67 |
| CviKI_1 | RGCY | 3 cut(s) | 15, 32, 67 |
| DpnI | GATC | 1 cut(s) | 51 |
| DpnII | GATC | 1 cut(s) | 49 |
| FaiI | YATR | 2 cut(s) | 54, 132 |
| FspBI | CTAG | 1 cut(s) | 29 |
| GsuI | CTGGAG | 1 cut(s) | 192 |
| HindIII | AAGCTT | 1 cut(s) | 13 |
| HinfI | GANTC | 1 cut(s) | 167 |
| Hpy166II | GTNNAC | 2 cut(s) | 9, 185 |
| Hpy188III | TCNNGA | 1 cut(s) | 171 |
| Hpy8I | GTNNAC | 2 cut(s) | 9, 185 |
| HpyAV | CCTTC | 1 cut(s) | 55 |
| Kzo9I | GATC | 1 cut(s) | 49 |
| LpnPI | CCDG | 1 cut(s) | 156 |
| LweI | GCATC | 1 cut(s) | 15 |
| MaeI | CTAG | 1 cut(s) | 29 |
| MalI | GATC | 1 cut(s) | 51 |
| MboI | GATC | 1 cut(s) | 49 |
| MluCI | AATT | 3 cut(s) | 135, 146, 162 |
| MmeI | TCCRAC | 1 cut(s) | 157 |
| MnlI | CCTC | 3 cut(s) | 104, 121, 173 |
| MseI | TTAA | 2 cut(s) | 18, 202 |
| NdeII | GATC | 1 cut(s) | 49 |
| NheI | GCTAGC | 1 cut(s) | 28 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 54 |
| PfeI | GAWTC | 1 cut(s) | 167 |
| SaqAI | TTAA | 2 cut(s) | 18, 202 |
| Sau3AI | GATC | 1 cut(s) | 49 |
| SetI | ASST | 2 cut(s) | 17, 184 |
| SfaNI | GCATC | 1 cut(s) | 15 |
| SgeI | CNNG | 5 cut(s) | 24, 34, 41, 130, 183 |
| Sse9I | AATT | 3 cut(s) | 135, 146, 162 |
| SspI | AATATT | 1 cut(s) | 200 |
| SspMI | CTAG | 1 cut(s) | 29 |
| TaqI | TCGA | 2 cut(s) | 48, 102 |
| TasI | AATT | 3 cut(s) | 135, 146, 162 |
| TfiI | GAWTC | 1 cut(s) | 167 |
| Tru1I | TTAA | 2 cut(s) | 18, 202 |
| Tru9I | TTAA | 2 cut(s) | 18, 202 |
| XspI | CTAG | 1 cut(s) | 29 |