RLG00000015523

60S ribosomal Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000904
Physical Location & Seq
Forward (+)
67460746 .. 67461349
604 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015523

Sequence Viewer

Length: 411 bp
ATGGTTTTCAAGACTGAGCTTTGCCACTTCAGTGGCGCCAAGATCTACCCTGGTAGGGGCATCAGATTCATCCGCTCCGATTCTCAGGTTTTCCTTTTCATAAATTCCAACTGCAAGAGGTACTTCCACAACCGCCTCAAGCCATCCAAGATCACATGGACTGCCATGTTCAGAAAGCAGCACAAGAAGGACTCTACTCAAGAATCTGTGAAGAAGAAGCGCCGTGCCACCAAGAAGCCTTACTCAAGGTCCATTGTTGGTGCCACTTTGGAGGTCATCCAGAAGAGGAGGACTGAGAAGCATGAGGTCCGTGACGCTGCTCGCGAGGCCGCTCTCTGGTATGTAATGCTATTATGTTTTGCTTCATTTGATGAACTTCATAGATTTGATTCTAGGTATTTTTCAACGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L24e

Protein Analysis

137

Amino Acids

16.22

Weight (kDa)

10.35

Isoelectric Point (pI)

46.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L24e PF01246 4 - 67 7.9e-27 Ribosomal protein L24e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 35, 260
AccBSI CCGCTC 2 cut(s) 75, 332
AccII CGCG 1 cut(s) 324
AciI CCGC 3 cut(s) 73, 133, 330
AcsI RAATTY 1 cut(s) 103
AcuI CTGAAG 1 cut(s) 13
AcyI GRCGYC 1 cut(s) 36
AfaI GTAC 1 cut(s) 122
AfiI CCNNNNNNNGG 3 cut(s) 55, 56, 336
AgsI TTSAA 2 cut(s) 10, 405
AjnI CCWGG 1 cut(s) 49
AleI CACNNNNGTG 1 cut(s) 30
AluBI AGCT 1 cut(s) 19
AluI AGCT 1 cut(s) 19
AoxI GGCC 1 cut(s) 327
ApeKI GCWGC 2 cut(s) 178, 317
ApoI RAATTY 1 cut(s) 103
AspLEI GCGC 2 cut(s) 38, 222
AspS9I GGNCC 2 cut(s) 249, 307
AvaII GGWCC 2 cut(s) 249, 307
BanI GGYRCC 2 cut(s) 35, 260
BbvI GCAGC 2 cut(s) 190, 304
BccI CCATC 1 cut(s) 151
BceAI ACGGC 1 cut(s) 207
BciT130I CCWGG 1 cut(s) 51
BfaI CTAG 1 cut(s) 393
BfoI RGCGCY 2 cut(s) 39, 223
BglII AGATCT 1 cut(s) 42
BisI GCNGC 3 cut(s) 179, 318, 330
BlsI GCNGC 3 cut(s) 180, 319, 331
Bme1390I CCNGG 1 cut(s) 51
Bme18I GGWCC 2 cut(s) 249, 307
BmgT120I GGNCC 2 cut(s) 249, 307
BmiI GGNNCC 2 cut(s) 37, 262
BmrFI CCNGG 1 cut(s) 51
BmsI GCATC 1 cut(s) 69
BpuEI CTTGAG 3 cut(s) 122, 183, 229
BsaHI GRCGYC 1 cut(s) 36
BsaJI CCNNGG 1 cut(s) 49
Bsc4I CCNNNNNNNGG 3 cut(s) 55, 56, 336
BseBI CCWGG 1 cut(s) 51
BseDI CCNNGG 1 cut(s) 49
BseGI GGATG 3 cut(s) 69, 143, 276
BseLI CCNNNNNNNGG 3 cut(s) 55, 56, 336
BseMII CTCAG 3 cut(s) 6, 98, 285
BseRI GAGGAG 1 cut(s) 301
BseXI GCAGC 2 cut(s) 190, 304
Bsh1236I CGCG 1 cut(s) 324
BshFI GGCC 1 cut(s) 329
BshNI GGYRCC 2 cut(s) 35, 260
BslI CCNNNNNNNGG 3 cut(s) 55, 56, 336
BsnI GGCC 1 cut(s) 329
Bsp143I GATC 2 cut(s) 42, 150
Bsp68I TCGCGA 1 cut(s) 324
BspACI CCGC 3 cut(s) 73, 133, 330
BspANI GGCC 1 cut(s) 329
BspCNI CTCAG 3 cut(s) 7, 97, 286
BspFNI CGCG 1 cut(s) 324
BspLI GGNNCC 2 cut(s) 37, 262
BspT107I GGYRCC 2 cut(s) 35, 260
BsrBI CCGCTC 2 cut(s) 75, 332
BssECI CCNNGG 1 cut(s) 49
BssMI GATC 2 cut(s) 42, 150
BssNI GRCGYC 1 cut(s) 36
Bst2UI CCWGG 1 cut(s) 51
Bst6I CTCTTC 1 cut(s) 278
BstACI GRCGYC 1 cut(s) 36
BstC8I GCNNGC 1 cut(s) 322
BstDEI CTNAG 3 cut(s) 15, 84, 294
BstF5I GGATG 3 cut(s) 69, 143, 276
BstFNI CGCG 1 cut(s) 324
BstH2I RGCGCY 2 cut(s) 39, 223
BstHHI GCGC 2 cut(s) 38, 222
BstKTI GATC 2 cut(s) 45, 153
BstMBI GATC 2 cut(s) 42, 150
BstMWI GCNNNNNNNGC 1 cut(s) 326
BstNI CCWGG 1 cut(s) 51
BstSCI CCNGG 1 cut(s) 49
BstUI CGCG 1 cut(s) 324
BstV1I GCAGC 2 cut(s) 190, 304
BstX2I RGATCY 1 cut(s) 42
BstXI CCANNNNNNTGG 1 cut(s) 32
BstYI RGATCY 1 cut(s) 42
BsuRI GGCC 1 cut(s) 329
BtsCI GGATG 3 cut(s) 69, 143, 276
BtsIMutI CAGTG 1 cut(s) 37
BtuMI TCGCGA 1 cut(s) 324
Cac8I GCNNGC 1 cut(s) 322
CfoI GCGC 2 cut(s) 38, 222
Cfr13I GGNCC 2 cut(s) 249, 307
CseI GACGC 1 cut(s) 323
Csp6I GTAC 1 cut(s) 121
CviAII CATG 3 cut(s) 156, 166, 302
CviJI RGCY 4 cut(s) 19, 142, 238, 329
CviKI_1 RGCY 4 cut(s) 19, 142, 238, 329
CviQI GTAC 1 cut(s) 121
DdeI CTNAG 3 cut(s) 15, 84, 294
DinI GGCGCC 1 cut(s) 37
DpnI GATC 2 cut(s) 44, 152
DpnII GATC 2 cut(s) 42, 150
Eam1104I CTCTTC 1 cut(s) 278
EarI CTCTTC 1 cut(s) 278
Eco47I GGWCC 2 cut(s) 249, 307
Eco57I CTGAAG 1 cut(s) 13
EcoRII CCWGG 1 cut(s) 49
EgeI GGCGCC 1 cut(s) 37
EheI GGCGCC 1 cut(s) 37
FaeI CATG 3 cut(s) 159, 169, 305
FaiI YATR 7 cut(s) 101, 157, 167, 303, 342, 355, 381
FalI AAGNNNNNCTT 2 cut(s) 107, 139
FatI CATG 3 cut(s) 155, 165, 301
Fnu4HI GCNGC 3 cut(s) 179, 318, 330
FokI GGATG 3 cut(s) 56, 130, 263
Fsp4HI GCNGC 3 cut(s) 179, 318, 330
FspBI CTAG 1 cut(s) 393
GlaI GCGC 2 cut(s) 37, 221
GluI GCNGC 3 cut(s) 179, 318, 330
HaeII RGCGCY 2 cut(s) 39, 223
HaeIII GGCC 1 cut(s) 329
HgaI GACGC 1 cut(s) 323
HhaI GCGC 2 cut(s) 38, 222
Hin1I GRCGYC 1 cut(s) 36
Hin1II CATG 3 cut(s) 159, 169, 305
Hin6I GCGC 2 cut(s) 36, 220
HinP1I GCGC 2 cut(s) 36, 220
HinfI GANTC 5 cut(s) 66, 80, 191, 203, 389
Hpy188I TCNGA 3 cut(s) 65, 79, 173
Hpy188III TCNNGA 4 cut(s) 10, 200, 280, 323
HpyAV CCTTC 1 cut(s) 181
HpyCH4IV ACGT 1 cut(s) 407
HpyCH4V TGCA 1 cut(s) 114
HpyF10VI GCNNNNNNNGC 1 cut(s) 326
HpyF3I CTNAG 3 cut(s) 15, 84, 294
HpySE526I ACGT 1 cut(s) 407
Hsp92I GRCGYC 1 cut(s) 36
Hsp92II CATG 3 cut(s) 159, 169, 305
HspAI GCGC 2 cut(s) 36, 220
KasI GGCGCC 1 cut(s) 35
Kzo9I GATC 2 cut(s) 42, 150
LmnI GCTCC 1 cut(s) 80
LpnPI CCDG 5 cut(s) 36, 63, 71, 293, 322
Lsp1109I GCAGC 2 cut(s) 190, 304
LweI GCATC 1 cut(s) 69
MaeI CTAG 1 cut(s) 393
MaeII ACGT 1 cut(s) 407
MaeIII GTNAC 1 cut(s) 311
MalI GATC 2 cut(s) 44, 152
MbiI CCGCTC 2 cut(s) 75, 332
MboI GATC 2 cut(s) 42, 150
MboII GAAGA 3 cut(s) 223, 226, 295
MflI RGATCY 1 cut(s) 42
MluCI AATT 1 cut(s) 103
Mly113I GGCGCC 1 cut(s) 36
MlyI GAGTC 1 cut(s) 185
MmeI TCCRAC 1 cut(s) 132
MnlI CCTC 7 cut(s) 111, 146, 265, 279, 282, 298, 319
MslI CAYNNNNRTG 1 cut(s) 30
MspR9I CCNGG 1 cut(s) 51
MvaI CCWGG 1 cut(s) 51
MvnI CGCG 1 cut(s) 324
MwoI GCNNNNNNNGC 1 cut(s) 326
NarI GGCGCC 1 cut(s) 36
NdeII GATC 2 cut(s) 42, 150
NlaIII CATG 3 cut(s) 159, 169, 305
NlaIV GGNNCC 2 cut(s) 37, 262
NmuCI GTSAC 1 cut(s) 311
NruI TCGCGA 1 cut(s) 324
OliI CACNNNNGTG 1 cut(s) 30
PcsI WCGNNNNNNNCGW 1 cut(s) 321
PfeI GAWTC 4 cut(s) 66, 80, 203, 389
PkrI GCNGC 3 cut(s) 180, 319, 331
PleI GAGTC 1 cut(s) 185
PluTI GGCGCC 1 cut(s) 39
PpsI GAGTC 1 cut(s) 185
Psp6I CCWGG 1 cut(s) 49
PspGI CCWGG 1 cut(s) 49
PspN4I GGNNCC 2 cut(s) 37, 262
PspPI GGNCC 2 cut(s) 249, 307
PsuI RGATCY 1 cut(s) 42
RruI TCGCGA 1 cut(s) 324
RsaI GTAC 1 cut(s) 122
RsaNI GTAC 1 cut(s) 121
RseI CAYNNNNRTG 1 cut(s) 30
SatI GCNGC 3 cut(s) 179, 318, 330
Sau3AI GATC 2 cut(s) 42, 150
Sau96I GGNCC 2 cut(s) 249, 307
SchI GAGTC 1 cut(s) 185
ScrFI CCNGG 1 cut(s) 51
SetI ASST 8 cut(s) 21, 90, 122, 251, 276, 309, 398, 410
SfaNI GCATC 1 cut(s) 69
SfoI GGCGCC 1 cut(s) 37
SinI GGWCC 2 cut(s) 249, 307
SmiMI CAYNNNNRTG 1 cut(s) 30
SmlI CTYRAG 3 cut(s) 137, 198, 244
SmoI CTYRAG 3 cut(s) 137, 198, 244
Sse9I AATT 1 cut(s) 103
SsiI CCGC 3 cut(s) 73, 133, 330
SspDI GGCGCC 1 cut(s) 35
SspMI CTAG 1 cut(s) 393
StyD4I CCNGG 1 cut(s) 49
TaiI ACGT 1 cut(s) 410
TasI AATT 1 cut(s) 103
TauI GCSGC 1 cut(s) 332
TfiI GAWTC 4 cut(s) 66, 80, 203, 389
TscAI CASTG 1 cut(s) 37
TseFI GTSAC 1 cut(s) 311
TseI GCWGC 2 cut(s) 178, 317
Tsp45I GTSAC 1 cut(s) 311
TspDTI ATGAA 5 cut(s) 58, 88, 354, 368, 387
TspGWI ACGGA 1 cut(s) 299
TspRI CASTG 1 cut(s) 37
VpaK11BI GGWCC 2 cut(s) 249, 307
XapI RAATTY 1 cut(s) 103
XspI CTAG 1 cut(s) 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.