Rroxscaffold_1G00076100

60S ribosomal Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000904
Physical Location & Seq
Reverse (-)
96638464 .. 96640957
2494 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00076100.1

Sequence Viewer

Length: 594 bp
ATGTCGTCCCGACACGTCTTCCTCCGGGAACCGCCGAGGCAGCCCATGGTTCTCACGACTAAGCTTTTCTTCGGTGGCGCCAAGATCTACCCCGGTAGGGGCATCGCATTCATCCACTCCGATTCTCGGGTTAAAACTTCACCTCTCCTTAATGGATTCCTATACTCATCGTCTTCACCGCTAATAATCCCGGACTTGTGGCTTGGTTTAGTTGCTTTGATTTCTTTCGATTCGATTGATTCGATCGATGATGAGTTTTGGTGTTATGTGACTACTACTACCGTAGGACTCGCTCAAGAATCCGTGAAGAAGCGTCGTGCCACCAAGAAGCCTTACTCAAGGTCCATTGTTGGTGCCACTTTGGAGGTCATCCGTAAGAGAGGACCGAGAAGCCTCGAGGTTTGTGATGCTGCTCGAGAGGCCGCTCTCTCGCATGCTTACAACCCACATGAGGAGTATGCAGATGTGGAATCCACATTGCGTTCGCATTTGGAATCTTTTCTAGAAACTGCAAGATCATTCAATATGATTTACACCAATGTCTGGTTTGCTTTCCCTTTGAGATACCGCCTTGACAATCTGCTCTTACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L24e

Protein Analysis

197

Amino Acids

22.36

Weight (kDa)

9.12

Isoelectric Point (pI)

51.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Plant_NMP1 PF06694 134 - 180 2.6e-12 Plant nuclear matrix protein 1 (NMP1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 395
AccB1I GGYRCC 2 cut(s) 77, 353
AccB7I CCANNNNNTGG 1 cut(s) 543
AccBSI CCGCTC 1 cut(s) 425
AciI CCGC 4 cut(s) 32, 179, 423, 568
AcyI GRCGYC 1 cut(s) 78
AfiI CCNNNNNNNGG 5 cut(s) 97, 98, 126, 451, 543
AflIII ACRYGT 1 cut(s) 13
AgsI TTSAA 1 cut(s) 523
AjiI CACGTC 1 cut(s) 16
AluBI AGCT 1 cut(s) 64
AluI AGCT 1 cut(s) 64
Ama87I CYCGRG 3 cut(s) 126, 395, 414
AoxI GGCC 1 cut(s) 420
ApeKI GCWGC 2 cut(s) 40, 410
Asp700I GAANNNNTTC 1 cut(s) 498
AspLEI GCGC 1 cut(s) 80
AspS9I GGNCC 2 cut(s) 342, 383
AsuC2I CCSGG 3 cut(s) 26, 93, 191
AsuHPI GGTGA 2 cut(s) 132, 168
AvaI CYCGRG 3 cut(s) 126, 395, 414
AvaII GGWCC 2 cut(s) 342, 383
BanI GGYRCC 2 cut(s) 77, 353
BbsI GAAGAC 2 cut(s) 10, 165
BbvI GCAGC 2 cut(s) 52, 397
BcnI CCSGG 3 cut(s) 26, 93, 191
BfaI CTAG 1 cut(s) 503
BfoI RGCGCY 1 cut(s) 81
BglII AGATCT 1 cut(s) 84
BisI GCNGC 3 cut(s) 41, 411, 423
BlsI GCNGC 3 cut(s) 42, 412, 424
Bme1390I CCNGG 3 cut(s) 26, 93, 191
Bme18I GGWCC 2 cut(s) 342, 383
BmeT110I CYCGRG 3 cut(s) 126, 395, 414
BmgBI CACGTC 1 cut(s) 16
BmgT120I GGNCC 2 cut(s) 342, 383
BmiI GGNNCC 3 cut(s) 30, 79, 355
BmrFI CCNGG 3 cut(s) 26, 93, 191
BmsI GCATC 2 cut(s) 111, 397
BpiI GAAGAC 2 cut(s) 10, 165
BpuEI CTTGAG 2 cut(s) 279, 322
BpuMI CCSGG 3 cut(s) 26, 93, 191
Bsa29I ATCGAT 1 cut(s) 246
BsaHI GRCGYC 1 cut(s) 78
BsaJI CCNNGG 3 cut(s) 35, 45, 91
Bsc4I CCNNNNNNNGG 5 cut(s) 97, 98, 126, 451, 543
Bse3DI GCAATG 1 cut(s) 476
BseCI ATCGAT 1 cut(s) 246
BseDI CCNNGG 3 cut(s) 35, 45, 91
BseGI GGATG 2 cut(s) 111, 369
BseLI CCNNNNNNNGG 5 cut(s) 97, 98, 126, 451, 543
BseMI GCAATG 1 cut(s) 476
BseRI GAGGAG 1 cut(s) 467
BseXI GCAGC 2 cut(s) 52, 397
Bsh1285I CGRYCG 1 cut(s) 246
BshFI GGCC 1 cut(s) 422
BshNI GGYRCC 2 cut(s) 77, 353
BshVI ATCGAT 1 cut(s) 246
BsiEI CGRYCG 1 cut(s) 246
BsiHKCI CYCGRG 3 cut(s) 126, 395, 414
BsiSI CCGG 3 cut(s) 25, 93, 191
BslI CCNNNNNNNGG 5 cut(s) 97, 98, 126, 451, 543
BsmI GAATGC 1 cut(s) 107
BsnI GGCC 1 cut(s) 422
BsoBI CYCGRG 3 cut(s) 126, 395, 414
Bsp143I GATC 3 cut(s) 84, 243, 515
Bsp19I CCATGG 1 cut(s) 45
BspACI CCGC 4 cut(s) 32, 179, 423, 568
BspANI GGCC 1 cut(s) 422
BspDI ATCGAT 1 cut(s) 246
BspLI GGNNCC 3 cut(s) 30, 79, 355
BspT107I GGYRCC 2 cut(s) 77, 353
BsrBI CCGCTC 1 cut(s) 425
BsrDI GCAATG 1 cut(s) 476
BssECI CCNNGG 3 cut(s) 35, 45, 91
BssMI GATC 3 cut(s) 84, 243, 515
BssNI GRCGYC 1 cut(s) 78
BssT1I CCWWGG 1 cut(s) 45
Bst4CI ACNGT 1 cut(s) 283
BstACI GRCGYC 1 cut(s) 78
BstC8I GCNNGC 1 cut(s) 435
BstDEI CTNAG 1 cut(s) 60
BstDSI CCRYGG 1 cut(s) 45
BstF5I GGATG 2 cut(s) 111, 369
BstH2I RGCGCY 1 cut(s) 81
BstHHI GCGC 1 cut(s) 80
BstKTI GATC 3 cut(s) 87, 246, 518
BstMBI GATC 3 cut(s) 84, 243, 515
BstMCI CGRYCG 1 cut(s) 246
BstMWI GCNNNNNNNGC 2 cut(s) 40, 419
BstNSI RCATGY 1 cut(s) 437
BstSCI CCNGG 3 cut(s) 24, 91, 189
BstV1I GCAGC 2 cut(s) 52, 397
BstV2I GAAGAC 2 cut(s) 10, 165
BstX2I RGATCY 1 cut(s) 84
BstYI RGATCY 1 cut(s) 84
Bsu15I ATCGAT 1 cut(s) 246
BsuRI GGCC 1 cut(s) 422
BsuTUI ATCGAT 1 cut(s) 246
BtgI CCRYGG 1 cut(s) 45
BtgZI GCGATG 1 cut(s) 88
BtrI CACGTC 1 cut(s) 16
BtsCI GGATG 2 cut(s) 111, 369
Cac8I GCNNGC 1 cut(s) 435
CfoI GCGC 1 cut(s) 80
Cfr13I GGNCC 2 cut(s) 342, 383
ClaI ATCGAT 1 cut(s) 246
CseI GACGC 1 cut(s) 302
CviAII CATG 3 cut(s) 46, 434, 449
CviJI RGCY 6 cut(s) 43, 64, 202, 331, 393, 422
CviKI_1 RGCY 6 cut(s) 43, 64, 202, 331, 393, 422
DdeI CTNAG 1 cut(s) 60
DinI GGCGCC 1 cut(s) 79
DpnI GATC 3 cut(s) 86, 245, 517
DpnII GATC 3 cut(s) 84, 243, 515
Eco130I CCWWGG 1 cut(s) 45
Eco47I GGWCC 2 cut(s) 342, 383
Eco88I CYCGRG 3 cut(s) 126, 395, 414
EcoT14I CCWWGG 1 cut(s) 45
EgeI GGCGCC 1 cut(s) 79
EheI GGCGCC 1 cut(s) 79
ErhI CCWWGG 1 cut(s) 45
FaeI CATG 3 cut(s) 49, 437, 452
FaiI YATR 7 cut(s) 47, 163, 267, 435, 450, 459, 527
FalI AAGNNNNNCTT 2 cut(s) 53, 85
FatI CATG 3 cut(s) 45, 433, 448
Fnu4HI GCNGC 3 cut(s) 41, 411, 423
FokI GGATG 2 cut(s) 98, 356
Fsp4HI GCNGC 3 cut(s) 41, 411, 423
FspBI CTAG 1 cut(s) 503
GlaI GCGC 1 cut(s) 79
GluI GCNGC 3 cut(s) 41, 411, 423
HaeII RGCGCY 1 cut(s) 81
HaeIII GGCC 1 cut(s) 422
HapII CCGG 3 cut(s) 25, 93, 191
HgaI GACGC 1 cut(s) 302
HhaI GCGC 1 cut(s) 80
Hin1I GRCGYC 1 cut(s) 78
Hin1II CATG 3 cut(s) 49, 437, 452
Hin6I GCGC 1 cut(s) 78
HinP1I GCGC 1 cut(s) 78
HindIII AAGCTT 1 cut(s) 62
HinfI GANTC 8 cut(s) 122, 156, 230, 239, 288, 299, 470, 494
HpaII CCGG 3 cut(s) 25, 93, 191
HphI GGTGA 2 cut(s) 132, 168
Hpy188I TCNGA 1 cut(s) 121
Hpy188III TCNNGA 5 cut(s) 9, 55, 296, 416, 503
Hpy99I CGWCG 1 cut(s) 318
HpyCH4III ACNGT 1 cut(s) 283
HpyCH4IV ACGT 1 cut(s) 15
HpyCH4V TGCA 2 cut(s) 461, 512
HpyF10VI GCNNNNNNNGC 2 cut(s) 40, 419
HpyF3I CTNAG 1 cut(s) 60
HpySE526I ACGT 1 cut(s) 15
Hsp92I GRCGYC 1 cut(s) 78
Hsp92II CATG 3 cut(s) 49, 437, 452
HspAI GCGC 1 cut(s) 78
KasI GGCGCC 1 cut(s) 77
Kzo9I GATC 3 cut(s) 84, 243, 515
LpnPI CCDG 4 cut(s) 38, 106, 204, 529
Lsp1109I GCAGC 2 cut(s) 52, 397
LweI GCATC 2 cut(s) 111, 397
MaeI CTAG 1 cut(s) 503
MaeII ACGT 1 cut(s) 15
MaeIII GTNAC 1 cut(s) 268
MalI GATC 3 cut(s) 86, 245, 517
MbiI CCGCTC 1 cut(s) 425
MboI GATC 3 cut(s) 84, 243, 515
MboII GAAGA 4 cut(s) 10, 61, 165, 319
MflI RGATCY 1 cut(s) 84
Mly113I GGCGCC 1 cut(s) 78
MlyI GAGTC 1 cut(s) 282
MnlI CCTC 9 cut(s) 30, 32, 153, 358, 374, 391, 404, 412, 445
MroXI GAANNNNTTC 1 cut(s) 498
MseI TTAA 3 cut(s) 132, 150, 592
MspI CCGG 3 cut(s) 25, 93, 191
MspR9I CCNGG 3 cut(s) 26, 93, 191
Mva1269I GAATGC 1 cut(s) 107
MwoI GCNNNNNNNGC 2 cut(s) 40, 419
NarI GGCGCC 1 cut(s) 78
NciI CCSGG 3 cut(s) 26, 93, 191
NcoI CCATGG 1 cut(s) 45
NdeII GATC 3 cut(s) 84, 243, 515
NlaIII CATG 3 cut(s) 49, 437, 452
NlaIV GGNNCC 3 cut(s) 30, 79, 355
NmeAIII GCCGAG 1 cut(s) 60
NmuCI GTSAC 1 cut(s) 268
NspI RCATGY 1 cut(s) 437
PaeI GCATGC 1 cut(s) 437
PaeR7I CTCGAG 2 cut(s) 395, 414
PcsI WCGNNNNNNNCGW 1 cut(s) 239
PctI GAATGC 1 cut(s) 107
PdmI GAANNNNTTC 1 cut(s) 498
PfeI GAWTC 7 cut(s) 122, 156, 230, 239, 299, 470, 494
PflMI CCANNNNNTGG 1 cut(s) 543
PfoI TCCNGGA 2 cut(s) 24, 189
PkrI GCNGC 3 cut(s) 42, 412, 424
Ple19I CGATCG 1 cut(s) 246
PleI GAGTC 1 cut(s) 282
PluTI GGCGCC 1 cut(s) 81
PpsI GAGTC 1 cut(s) 282
PspN4I GGNNCC 3 cut(s) 30, 79, 355
PspPI GGNCC 2 cut(s) 342, 383
PspXI VCTCGAGB 1 cut(s) 395
PsuI RGATCY 1 cut(s) 84
PvuI CGATCG 1 cut(s) 246
SaqAI TTAA 3 cut(s) 132, 150, 592
SatI GCNGC 3 cut(s) 41, 411, 423
Sau3AI GATC 3 cut(s) 84, 243, 515
Sau96I GGNCC 2 cut(s) 342, 383
SchI GAGTC 1 cut(s) 282
ScrFI CCNGG 3 cut(s) 26, 93, 191
SetI ASST 6 cut(s) 18, 66, 145, 344, 369, 402
SfaNI GCATC 2 cut(s) 111, 397
SfoI GGCGCC 1 cut(s) 79
Sfr274I CTCGAG 2 cut(s) 395, 414
SinI GGWCC 2 cut(s) 342, 383
SlaI CTCGAG 2 cut(s) 395, 414
SmlI CTYRAG 4 cut(s) 294, 337, 395, 414
SmoI CTYRAG 4 cut(s) 294, 337, 395, 414
SphI GCATGC 1 cut(s) 437
SsiI CCGC 4 cut(s) 32, 179, 423, 568
SspDI GGCGCC 1 cut(s) 77
SspMI CTAG 1 cut(s) 503
StyD4I CCNGG 3 cut(s) 24, 91, 189
StyI CCWWGG 1 cut(s) 45
TaaI ACNGT 1 cut(s) 283
TaiI ACGT 1 cut(s) 18
TaqI TCGA 6 cut(s) 228, 233, 242, 246, 396, 415
TaqII GACCGA 1 cut(s) 400
TauI GCSGC 1 cut(s) 425
TfiI GAWTC 7 cut(s) 122, 156, 230, 239, 299, 470, 494
Tru1I TTAA 3 cut(s) 132, 150, 592
Tru9I TTAA 3 cut(s) 132, 150, 592
TseFI GTSAC 1 cut(s) 268
TseI GCWGC 2 cut(s) 40, 410
Tsp45I GTSAC 1 cut(s) 268
TspDTI ATGAA 1 cut(s) 100
TspGWI ACGGA 2 cut(s) 292, 362
Van91I CCANNNNNTGG 1 cut(s) 543
VpaK11BI GGWCC 2 cut(s) 342, 383
XbaI TCTAGA 1 cut(s) 502
XceI RCATGY 1 cut(s) 437
XhoI CTCGAG 2 cut(s) 395, 414
XmnI GAANNNNTTC 1 cut(s) 498
XspI CTAG 1 cut(s) 503
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.