RLG00000017381

60S ribosomal Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000904
Physical Location & Seq
Forward (+)
15905987 .. 15906610
624 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017381

Sequence Viewer

Length: 411 bp
ATGGTTTTCAAGACTGAGCTTTGCCGCTTCAGTGGCTCCAAGATCTACCCTGGTAGGGGCATCAGATTCATCCGCTCCGATTCTCAGGTTTTCCTGTTCATAAATTCCAAGTGCAAGAGGTACTTCCACAACCGCCTCAAGCCATCCAAGATCACATGGACTGCCATGTTCAGAAAGCAGCACAAGAAGGACTCTGCTCAAGAATCTGTGAAGAAGAAGCGCCGTGCCACCAAGAAGCCTTACTCAAGGTCCATTGTTGGTGCCACTTTGGAGGTCATTCAGAAGAGGAGGACTGAGAAGCCAGAGGTCCGTGACGCTGCTCGCGAGGCCGCTCTCTGGTATGCAATGCTATTATGTTTTGCTTCATTTGATGAACTTCATAGATTTGATTGTAGGTGTTTTTCGACGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L24e

Protein Analysis

137

Amino Acids

16.12

Weight (kDa)

10.35

Isoelectric Point (pI)

46.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L24e PF01246 4 - 67 6.7e-28 Ribosomal protein L24e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 260
AccBSI CCGCTC 2 cut(s) 75, 332
AccII CGCG 1 cut(s) 324
AciI CCGC 4 cut(s) 25, 73, 133, 330
AcsI RAATTY 1 cut(s) 103
AcuI CTGAAG 1 cut(s) 13
AfaI GTAC 1 cut(s) 122
AfiI CCNNNNNNNGG 3 cut(s) 55, 56, 336
AgsI TTSAA 1 cut(s) 10
AjnI CCWGG 1 cut(s) 49
AluBI AGCT 1 cut(s) 19
AluI AGCT 1 cut(s) 19
AoxI GGCC 1 cut(s) 327
ApeKI GCWGC 2 cut(s) 178, 317
ApoI RAATTY 1 cut(s) 103
AspLEI GCGC 1 cut(s) 222
AspS9I GGNCC 2 cut(s) 249, 307
AvaII GGWCC 2 cut(s) 249, 307
BanI GGYRCC 1 cut(s) 260
BbvI GCAGC 2 cut(s) 190, 304
BccI CCATC 1 cut(s) 151
BceAI ACGGC 1 cut(s) 207
BciT130I CCWGG 1 cut(s) 51
BfoI RGCGCY 1 cut(s) 223
BglII AGATCT 1 cut(s) 42
BisI GCNGC 4 cut(s) 25, 179, 318, 330
BlsI GCNGC 4 cut(s) 26, 180, 319, 331
Bme1390I CCNGG 1 cut(s) 51
Bme18I GGWCC 2 cut(s) 249, 307
BmgT120I GGNCC 2 cut(s) 249, 307
BmiI GGNNCC 2 cut(s) 37, 262
BmrFI CCNGG 1 cut(s) 51
BmsI GCATC 1 cut(s) 69
BpuEI CTTGAG 3 cut(s) 122, 183, 229
BsaJI CCNNGG 1 cut(s) 49
Bsc4I CCNNNNNNNGG 3 cut(s) 55, 56, 336
Bse3DI GCAATG 1 cut(s) 351
BseBI CCWGG 1 cut(s) 51
BseDI CCNNGG 1 cut(s) 49
BseGI GGATG 2 cut(s) 69, 143
BseLI CCNNNNNNNGG 3 cut(s) 55, 56, 336
BseMI GCAATG 1 cut(s) 351
BseMII CTCAG 3 cut(s) 6, 98, 285
BseRI GAGGAG 1 cut(s) 301
BseXI GCAGC 2 cut(s) 190, 304
Bsh1236I CGCG 1 cut(s) 324
BshFI GGCC 1 cut(s) 329
BshNI GGYRCC 1 cut(s) 260
BslI CCNNNNNNNGG 3 cut(s) 55, 56, 336
BsnI GGCC 1 cut(s) 329
Bsp143I GATC 2 cut(s) 42, 150
Bsp68I TCGCGA 1 cut(s) 324
BspACI CCGC 4 cut(s) 25, 73, 133, 330
BspANI GGCC 1 cut(s) 329
BspCNI CTCAG 3 cut(s) 7, 97, 286
BspFNI CGCG 1 cut(s) 324
BspLI GGNNCC 2 cut(s) 37, 262
BspT107I GGYRCC 1 cut(s) 260
BsrBI CCGCTC 2 cut(s) 75, 332
BsrDI GCAATG 1 cut(s) 351
BssECI CCNNGG 1 cut(s) 49
BssMI GATC 2 cut(s) 42, 150
Bst2UI CCWGG 1 cut(s) 51
Bst6I CTCTTC 1 cut(s) 278
BstC8I GCNNGC 1 cut(s) 322
BstDEI CTNAG 3 cut(s) 15, 84, 294
BstF5I GGATG 2 cut(s) 69, 143
BstFNI CGCG 1 cut(s) 324
BstH2I RGCGCY 1 cut(s) 223
BstHHI GCGC 1 cut(s) 222
BstKTI GATC 2 cut(s) 45, 153
BstMBI GATC 2 cut(s) 42, 150
BstMWI GCNNNNNNNGC 2 cut(s) 33, 326
BstNI CCWGG 1 cut(s) 51
BstSCI CCNGG 1 cut(s) 49
BstUI CGCG 1 cut(s) 324
BstV1I GCAGC 2 cut(s) 190, 304
BstX2I RGATCY 1 cut(s) 42
BstYI RGATCY 1 cut(s) 42
BsuRI GGCC 1 cut(s) 329
BtsCI GGATG 2 cut(s) 69, 143
BtsIMutI CAGTG 1 cut(s) 37
BtuMI TCGCGA 1 cut(s) 324
Cac8I GCNNGC 1 cut(s) 322
CfoI GCGC 1 cut(s) 222
Cfr13I GGNCC 2 cut(s) 249, 307
CseI GACGC 1 cut(s) 323
Csp6I GTAC 1 cut(s) 121
CviAII CATG 2 cut(s) 156, 166
CviJI RGCY 6 cut(s) 19, 36, 142, 238, 301, 329
CviKI_1 RGCY 6 cut(s) 19, 36, 142, 238, 301, 329
CviQI GTAC 1 cut(s) 121
DdeI CTNAG 3 cut(s) 15, 84, 294
DpnI GATC 2 cut(s) 44, 152
DpnII GATC 2 cut(s) 42, 150
Eam1104I CTCTTC 1 cut(s) 278
EarI CTCTTC 1 cut(s) 278
Eco47I GGWCC 2 cut(s) 249, 307
Eco57I CTGAAG 1 cut(s) 13
EcoRII CCWGG 1 cut(s) 49
FaeI CATG 2 cut(s) 159, 169
FaiI YATR 6 cut(s) 101, 157, 167, 342, 355, 381
FalI AAGNNNNNCTT 2 cut(s) 107, 139
FatI CATG 2 cut(s) 155, 165
Fnu4HI GCNGC 4 cut(s) 25, 179, 318, 330
FokI GGATG 2 cut(s) 56, 130
Fsp4HI GCNGC 4 cut(s) 25, 179, 318, 330
GlaI GCGC 1 cut(s) 221
GluI GCNGC 4 cut(s) 25, 179, 318, 330
HaeII RGCGCY 1 cut(s) 223
HaeIII GGCC 1 cut(s) 329
HgaI GACGC 1 cut(s) 323
HhaI GCGC 1 cut(s) 222
Hin1II CATG 2 cut(s) 159, 169
Hin6I GCGC 1 cut(s) 220
HinP1I GCGC 1 cut(s) 220
HinfI GANTC 4 cut(s) 66, 80, 191, 203
Hpy188I TCNGA 4 cut(s) 65, 79, 173, 282
Hpy188III TCNNGA 3 cut(s) 10, 200, 323
Hpy99I CGWCG 1 cut(s) 409
HpyAV CCTTC 1 cut(s) 181
HpyCH4IV ACGT 1 cut(s) 407
HpyCH4V TGCA 2 cut(s) 114, 344
HpyF10VI GCNNNNNNNGC 2 cut(s) 33, 326
HpyF3I CTNAG 3 cut(s) 15, 84, 294
HpySE526I ACGT 1 cut(s) 407
Hsp92II CATG 2 cut(s) 159, 169
HspAI GCGC 1 cut(s) 220
Kzo9I GATC 2 cut(s) 42, 150
LmnI GCTCC 2 cut(s) 41, 80
LpnPI CCDG 6 cut(s) 36, 63, 71, 107, 315, 322
Lsp1109I GCAGC 2 cut(s) 190, 304
LweI GCATC 1 cut(s) 69
MaeII ACGT 1 cut(s) 407
MaeIII GTNAC 1 cut(s) 311
MalI GATC 2 cut(s) 44, 152
MbiI CCGCTC 2 cut(s) 75, 332
MboI GATC 2 cut(s) 42, 150
MboII GAAGA 3 cut(s) 223, 226, 295
MflI RGATCY 1 cut(s) 42
MluCI AATT 1 cut(s) 103
MlyI GAGTC 1 cut(s) 185
MnlI CCTC 7 cut(s) 111, 146, 265, 279, 282, 298, 319
MspR9I CCNGG 1 cut(s) 51
MvaI CCWGG 1 cut(s) 51
MvnI CGCG 1 cut(s) 324
MwoI GCNNNNNNNGC 2 cut(s) 33, 326
NdeII GATC 2 cut(s) 42, 150
NlaIII CATG 2 cut(s) 159, 169
NlaIV GGNNCC 2 cut(s) 37, 262
NmuCI GTSAC 1 cut(s) 311
NruI TCGCGA 1 cut(s) 324
PcsI WCGNNNNNNNCGW 1 cut(s) 321
PfeI GAWTC 3 cut(s) 66, 80, 203
PkrI GCNGC 4 cut(s) 26, 180, 319, 331
PleI GAGTC 1 cut(s) 185
PpsI GAGTC 1 cut(s) 185
Psp6I CCWGG 1 cut(s) 49
PspGI CCWGG 1 cut(s) 49
PspN4I GGNNCC 2 cut(s) 37, 262
PspPI GGNCC 2 cut(s) 249, 307
PsuI RGATCY 1 cut(s) 42
RruI TCGCGA 1 cut(s) 324
RsaI GTAC 1 cut(s) 122
RsaNI GTAC 1 cut(s) 121
SatI GCNGC 4 cut(s) 25, 179, 318, 330
Sau3AI GATC 2 cut(s) 42, 150
Sau96I GGNCC 2 cut(s) 249, 307
SchI GAGTC 1 cut(s) 185
ScrFI CCNGG 1 cut(s) 51
SetI ASST 8 cut(s) 21, 90, 122, 251, 276, 309, 398, 410
SfaNI GCATC 1 cut(s) 69
SinI GGWCC 2 cut(s) 249, 307
SmlI CTYRAG 3 cut(s) 137, 198, 244
SmoI CTYRAG 3 cut(s) 137, 198, 244
Sse9I AATT 1 cut(s) 103
SsiI CCGC 4 cut(s) 25, 73, 133, 330
StyD4I CCNGG 1 cut(s) 49
TaiI ACGT 1 cut(s) 410
TaqI TCGA 1 cut(s) 404
TasI AATT 1 cut(s) 103
TauI GCSGC 2 cut(s) 27, 332
TfiI GAWTC 3 cut(s) 66, 80, 203
TscAI CASTG 1 cut(s) 37
TseFI GTSAC 1 cut(s) 311
TseI GCWGC 2 cut(s) 178, 317
Tsp45I GTSAC 1 cut(s) 311
TspDTI ATGAA 5 cut(s) 58, 88, 354, 368, 387
TspGWI ACGGA 1 cut(s) 299
TspRI CASTG 1 cut(s) 37
VpaK11BI GGWCC 2 cut(s) 249, 307
XapI RAATTY 1 cut(s) 103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.