Rroxscaffold_4G00281660

60S ribosomal Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000904
Physical Location & Seq
Forward (+)
3877518 .. 3879229
1712 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_4G00281660.1

Sequence Viewer

Length: 399 bp
ATGGTTCTCAAGACTGAACTATGCCGCTTCAGTGGGGCTAAGATCTACCCAGGTAGAGGCATCCGATTTGTTCGTGGGGATTCTCAGGACATTTCTCAAGAAACTGTTAAGAAGAGGCGTCGCACCACGAAAAAGCCTTACTCTCGATCCATAGTGGGTGCTACTTTGGAGGTCATACAGAAGAGAAGAAGTGAAAAACCTGAAGTTCGAGATGCTGCAAGAGAGGCTGCTCTTCGTGAAATTAAGGAGAGGATCAAGAAAACCAAGGATGAAAAGAGGGCGAAGAAGGCCGAAGTAATGGCCAGGTCAACAAAGAATCAAAGCAAGGGTAACTTGCCAAAGGGTGGTGCTGCAAATAAGGGCCCCAAGGGCGCTGGAGGAGCTAAGGGCAAGCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L24e

Protein Analysis

132

Amino Acids

14.69

Weight (kDa)

10.96

Isoelectric Point (pI)

53.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L24e PF01246 3 - 44 6.2e-10 Ribosomal protein L24e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 344
AciI CCGC 1 cut(s) 25
AclWI GGATC 2 cut(s) 141, 260
AcoI YGGCCR 1 cut(s) 300
AcuI CTGAAG 2 cut(s) 13, 222
AcyI GRCGYC 1 cut(s) 118
AfiI CCNNNNNNNGG 2 cut(s) 56, 344
AjnI CCWGG 2 cut(s) 49, 302
AluBI AGCT 1 cut(s) 383
AluI AGCT 1 cut(s) 383
AlwI GGATC 2 cut(s) 141, 260
AoxI GGCC 3 cut(s) 288, 300, 361
ApaI GGGCCC 1 cut(s) 365
ApeKI GCWGC 3 cut(s) 215, 227, 350
AspLEI GCGC 1 cut(s) 374
AspS9I GGNCC 2 cut(s) 361, 362
BaeGI GKGCMC 1 cut(s) 365
BalI TGGCCA 1 cut(s) 302
BanII GRGCYC 1 cut(s) 365
BbvI GCAGC 3 cut(s) 202, 214, 337
BciT130I CCWGG 2 cut(s) 51, 304
BfoI RGCGCY 1 cut(s) 375
BglI GCCNNNNNGGC 1 cut(s) 369
BglII AGATCT 1 cut(s) 42
BisI GCNGC 4 cut(s) 25, 216, 228, 351
BlsI GCNGC 4 cut(s) 26, 217, 229, 352
Bme1390I CCNGG 2 cut(s) 51, 304
BmgT120I GGNCC 2 cut(s) 361, 362
BmiI GGNNCC 2 cut(s) 363, 364
BmrFI CCNGG 2 cut(s) 51, 304
BmsI GCATC 2 cut(s) 69, 202
BpmI CTGGAG 1 cut(s) 396
Bpu10I CCTNAGC 1 cut(s) 384
BpuEI CTTGAG 1 cut(s) 81
BsaHI GRCGYC 1 cut(s) 118
BsaJI CCNNGG 3 cut(s) 49, 264, 366
Bsc4I CCNNNNNNNGG 2 cut(s) 56, 344
BseBI CCWGG 2 cut(s) 51, 304
BseDI CCNNGG 3 cut(s) 49, 264, 366
BseGI GGATG 2 cut(s) 60, 274
BseLI CCNNNNNNNGG 2 cut(s) 56, 344
BseMII CTCAG 1 cut(s) 98
BseRI GAGGAG 1 cut(s) 393
BseSI GKGCMC 1 cut(s) 365
BseXI GCAGC 3 cut(s) 202, 214, 337
BshFI GGCC 3 cut(s) 290, 302, 363
BslI CCNNNNNNNGG 2 cut(s) 56, 344
BsnI GGCC 3 cut(s) 290, 302, 363
Bsp120I GGGCCC 1 cut(s) 361
Bsp1286I GDGCHC 1 cut(s) 365
Bsp143I GATC 3 cut(s) 42, 146, 252
BspACI CCGC 1 cut(s) 25
BspANI GGCC 3 cut(s) 290, 302, 363
BspCNI CTCAG 1 cut(s) 97
BspLI GGNNCC 2 cut(s) 363, 364
BspPI GGATC 2 cut(s) 141, 260
BspQI GCTCTTC 1 cut(s) 237
BssECI CCNNGG 3 cut(s) 49, 264, 366
BssMI GATC 3 cut(s) 42, 146, 252
BssNI GRCGYC 1 cut(s) 118
BssT1I CCWWGG 2 cut(s) 264, 366
Bst2UI CCWGG 2 cut(s) 51, 304
Bst4CI ACNGT 1 cut(s) 106
Bst6I CTCTTC 3 cut(s) 107, 176, 237
BstACI GRCGYC 1 cut(s) 118
BstC8I GCNNGC 1 cut(s) 392
BstDEI CTNAG 3 cut(s) 39, 84, 384
BstF5I GGATG 2 cut(s) 60, 274
BstH2I RGCGCY 1 cut(s) 375
BstHHI GCGC 1 cut(s) 374
BstKTI GATC 3 cut(s) 45, 149, 255
BstMBI GATC 3 cut(s) 42, 146, 252
BstMWI GCNNNNNNNGC 4 cut(s) 224, 287, 369, 380
BstNI CCWGG 2 cut(s) 51, 304
BstSCI CCNGG 2 cut(s) 49, 302
BstSLI GKGCMC 1 cut(s) 365
BstV1I GCAGC 3 cut(s) 202, 214, 337
BstX2I RGATCY 1 cut(s) 42
BstYI RGATCY 1 cut(s) 42
BsuRI GGCC 3 cut(s) 290, 302, 363
BtsCI GGATG 2 cut(s) 60, 274
BtsIMutI CAGTG 1 cut(s) 37
Cac8I GCNNGC 1 cut(s) 392
CfoI GCGC 1 cut(s) 374
Cfr13I GGNCC 2 cut(s) 361, 362
CseI GACGC 1 cut(s) 107
CviJI RGCY 7 cut(s) 38, 136, 227, 290, 302, 363, 383
CviKI_1 RGCY 7 cut(s) 38, 136, 227, 290, 302, 363, 383
DdeI CTNAG 3 cut(s) 39, 84, 384
DpnI GATC 3 cut(s) 44, 148, 254
DpnII GATC 3 cut(s) 42, 146, 252
EaeI YGGCCR 1 cut(s) 300
Eam1104I CTCTTC 3 cut(s) 107, 176, 237
EarI CTCTTC 3 cut(s) 107, 176, 237
Eco130I CCWWGG 2 cut(s) 264, 366
Eco24I GRGCYC 1 cut(s) 365
Eco57I CTGAAG 2 cut(s) 13, 222
EcoO109I RGGNCCY 2 cut(s) 361, 362
EcoRII CCWGG 2 cut(s) 49, 302
EcoT14I CCWWGG 2 cut(s) 264, 366
EcoT38I GRGCYC 1 cut(s) 365
ErhI CCWWGG 2 cut(s) 264, 366
FaiI YATR 3 cut(s) 22, 152, 176
FalI AAGNNNNNCTT 2 cut(s) 317, 349
Fnu4HI GCNGC 4 cut(s) 25, 216, 228, 351
FokI GGATG 2 cut(s) 47, 281
FriOI GRGCYC 1 cut(s) 365
Fsp4HI GCNGC 4 cut(s) 25, 216, 228, 351
GlaI GCGC 1 cut(s) 373
GluI GCNGC 4 cut(s) 25, 216, 228, 351
GsuI CTGGAG 1 cut(s) 396
HaeII RGCGCY 1 cut(s) 375
HaeIII GGCC 3 cut(s) 290, 302, 363
HgaI GACGC 1 cut(s) 107
HhaI GCGC 1 cut(s) 374
Hin1I GRCGYC 1 cut(s) 118
Hin6I GCGC 1 cut(s) 372
HinP1I GCGC 1 cut(s) 372
HincII GTYRAC 1 cut(s) 309
HindII GTYRAC 1 cut(s) 309
HinfI GANTC 2 cut(s) 80, 316
Hpy166II GTNNAC 1 cut(s) 309
Hpy188I TCNGA 1 cut(s) 65
Hpy188III TCNNGA 7 cut(s) 10, 86, 98, 144, 209, 236, 256
Hpy8I GTNNAC 1 cut(s) 309
Hpy99I CGWCG 1 cut(s) 123
HpyAV CCTTC 1 cut(s) 280
HpyCH4III ACNGT 1 cut(s) 106
HpyCH4V TGCA 2 cut(s) 218, 353
HpyF10VI GCNNNNNNNGC 4 cut(s) 224, 287, 369, 380
HpyF3I CTNAG 3 cut(s) 39, 84, 384
Hsp92I GRCGYC 1 cut(s) 118
HspAI GCGC 1 cut(s) 372
Kzo9I GATC 3 cut(s) 42, 146, 252
LguI GCTCTTC 1 cut(s) 237
LmnI GCTCC 1 cut(s) 380
LpnPI CCDG 7 cut(s) 36, 63, 71, 213, 289, 316, 360
Lsp1109I GCAGC 3 cut(s) 202, 214, 337
LweI GCATC 2 cut(s) 69, 202
MaeIII GTNAC 1 cut(s) 329
MalI GATC 3 cut(s) 44, 148, 254
MboI GATC 3 cut(s) 42, 146, 252
MboII GAAGA 5 cut(s) 124, 193, 198, 224, 295
MflI RGATCY 1 cut(s) 42
MhlI GDGCHC 1 cut(s) 365
MlsI TGGCCA 1 cut(s) 302
MluCI AATT 1 cut(s) 240
MluNI TGGCCA 1 cut(s) 302
MnlI CCTC 7 cut(s) 50, 108, 163, 217, 243, 270, 371
Mox20I TGGCCA 1 cut(s) 302
MscI TGGCCA 1 cut(s) 302
MseI TTAA 2 cut(s) 108, 243
Msp20I TGGCCA 1 cut(s) 302
MspR9I CCNGG 2 cut(s) 51, 304
MvaI CCWGG 2 cut(s) 51, 304
MwoI GCNNNNNNNGC 4 cut(s) 224, 287, 369, 380
NdeII GATC 3 cut(s) 42, 146, 252
NlaIV GGNNCC 2 cut(s) 363, 364
PciSI GCTCTTC 1 cut(s) 237
PfeI GAWTC 2 cut(s) 80, 316
PflMI CCANNNNNTGG 1 cut(s) 344
PkrI GCNGC 4 cut(s) 26, 217, 229, 352
Psp6I CCWGG 2 cut(s) 49, 302
PspGI CCWGG 2 cut(s) 49, 302
PspN4I GGNNCC 2 cut(s) 363, 364
PspOMI GGGCCC 1 cut(s) 361
PspPI GGNCC 2 cut(s) 361, 362
PsuI RGATCY 1 cut(s) 42
SapI GCTCTTC 1 cut(s) 237
SaqAI TTAA 2 cut(s) 108, 243
SatI GCNGC 4 cut(s) 25, 216, 228, 351
Sau3AI GATC 3 cut(s) 42, 146, 252
Sau96I GGNCC 2 cut(s) 361, 362
ScrFI CCNGG 2 cut(s) 51, 304
SduI GDGCHC 1 cut(s) 365
SetI ASST 5 cut(s) 55, 174, 202, 308, 385
SfaNI GCATC 2 cut(s) 69, 202
SmlI CTYRAG 2 cut(s) 8, 96
SmoI CTYRAG 2 cut(s) 8, 96
Sse9I AATT 1 cut(s) 240
SsiI CCGC 1 cut(s) 25
StyD4I CCNGG 2 cut(s) 49, 302
StyI CCWWGG 2 cut(s) 264, 366
TaaI ACNGT 1 cut(s) 106
TaqI TCGA 2 cut(s) 145, 208
TasI AATT 1 cut(s) 240
TauI GCSGC 1 cut(s) 27
TfiI GAWTC 2 cut(s) 80, 316
Tru1I TTAA 2 cut(s) 108, 243
Tru9I TTAA 2 cut(s) 108, 243
TscAI CASTG 1 cut(s) 37
TseI GCWGC 3 cut(s) 215, 227, 350
TspDTI ATGAA 1 cut(s) 285
TspRI CASTG 1 cut(s) 37
Van91I CCANNNNNTGG 1 cut(s) 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.