Rmu_sc0002505.1_g000024

EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002505.1
OG0001544
Physical Location & Seq
Forward (+)
101654 .. 104498
2845 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 894 bp
atggcctttgcagaaatcgacgtgcacaaccttccggtcaccctatccagcacaatagcacatttagaactgacttactcatactgttatggatgggatgacaaacttcaggcatatgcattaaatggaagaggattagttgggggaagtttccaacttgattaccctagcgttggggcaacagaaatccttctaatggctgcatgtatggctgatggaacaactatactgtccaatgttgccagagaaccggaggtggttgatcttgctcgatttcttactaaaagtggggcctgtgtggaaggagcaggaagcaacaagcttgtcatcaagggaaaatcttatttgcatggttgtaaatgtactattgcacctgatcgtattgaagcaggcacatttatgcttgctgcagctattactcgctcattcatttcaatttcacctgtcattccttcccaagtttcctgtctgatgcaaaaactcttggccgctggttgcaaaataaggcaatgctctcatgataccttggaagtttcagcagtgtctcaatgtggtgagaatttgcgaggttttgaggttaagacagggccatttcccgggtttcctacagatctccaaccgcaaacaatggcactgctaaccacttggtttaagtcctgtagaagtctctatttgaaaagtgcatggctcatggtatgtgaagcttggagcaaaaattcaagtctgtgcaagctctgctctggtttcgggaaagataatagaaatggtttgtctggttcctgccttgctgcaactgacctcagaggtgggatatcattggtattagctgcattggctgcagaaggtactactgagatcagcggtgttgctcatattgtggttatgagaatgtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPSP_synthase

Protein Analysis

297

Amino Acids

31.64

Weight (kDa)

7.45

Isoelectric Point (pI)

39.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPSP_synthase PF00275 43 - 292 1.8e-38 EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 489, 620, 861
AcoI YGGCCR 1 cut(s) 486
AcsI RAATTY 2 cut(s) 559, 715
AcuI CTGAAG 1 cut(s) 92
AfaI GTAC 2 cut(s) 364, 847
AfiI CCNNNNNNNGG 3 cut(s) 173, 196, 596
AgsI TTSAA 4 cut(s) 386, 435, 676, 720
AjiI CACGTC 1 cut(s) 22
AluBI AGCT 5 cut(s) 322, 413, 704, 733, 827
AluI AGCT 5 cut(s) 322, 413, 704, 733, 827
Alw21I GWGCWC 1 cut(s) 27
Alw26I GTCTC 2 cut(s) 549, 671
Alw44I GTGCAC 1 cut(s) 23
Ama87I CYCGRG 1 cut(s) 596
AoxI GGCC 4 cut(s) 3, 291, 486, 587
ApaLI GTGCAC 1 cut(s) 23
ApeKI GCWGC 6 cut(s) 200, 407, 410, 788, 827, 836
ApoI RAATTY 2 cut(s) 559, 715
Asp700I GAANNNNTTC 1 cut(s) 189
AspS9I GGNCC 2 cut(s) 291, 587
AsuC2I CCSGG 2 cut(s) 597, 598
AsuHPI GGTGA 3 cut(s) 31, 432, 566
AvaI CYCGRG 1 cut(s) 596
BaeGI GKGCMC 1 cut(s) 27
Bbv12I GWGCWC 1 cut(s) 27
BbvI GCAGC 6 cut(s) 187, 394, 422, 775, 814, 823
BccI CCATC 2 cut(s) 87, 209
BcnI CCSGG 2 cut(s) 597, 598
BcoDI GTCTC 2 cut(s) 549, 671
BfaI CTAG 1 cut(s) 168
BfmI CTRYAG 4 cut(s) 408, 606, 658, 837
BglII AGATCT 1 cut(s) 610
BisI GCNGC 7 cut(s) 201, 408, 411, 489, 789, 828, 837
BlsI GCNGC 7 cut(s) 202, 409, 412, 490, 790, 829, 838
Bme1390I CCNGG 2 cut(s) 597, 598
BmeT110I CYCGRG 1 cut(s) 596
BmgBI CACGTC 1 cut(s) 22
BmgT120I GGNCC 2 cut(s) 291, 587
BmiI GGNNCC 2 cut(s) 292, 778
BmrFI CCNGG 2 cut(s) 597, 598
BmsI GCATC 1 cut(s) 462
BpuMI CCSGG 2 cut(s) 597, 598
BsaJI CCNNGG 2 cut(s) 525, 596
BsaWI WCCGGW 2 cut(s) 34, 250
Bsc4I CCNNNNNNNGG 3 cut(s) 173, 196, 596
Bse3DI GCAATG 1 cut(s) 515
BseDI CCNNGG 2 cut(s) 525, 596
BseGI GGATG 2 cut(s) 98, 103
BseLI CCNNNNNNNGG 3 cut(s) 173, 196, 596
BseMI GCAATG 1 cut(s) 515
BseMII CTCAG 2 cut(s) 814, 843
BseSI GKGCMC 1 cut(s) 27
BseXI GCAGC 6 cut(s) 187, 394, 422, 775, 814, 823
BshFI GGCC 4 cut(s) 5, 293, 488, 589
BsiHKAI GWGCWC 1 cut(s) 27
BsiHKCI CYCGRG 1 cut(s) 596
BsiSI CCGG 3 cut(s) 35, 251, 597
BslI CCNNNNNNNGG 3 cut(s) 173, 196, 596
BsmAI GTCTC 2 cut(s) 549, 671
BsnI GGCC 4 cut(s) 5, 293, 488, 589
BsoBI CYCGRG 1 cut(s) 596
Bsp1286I GDGCHC 1 cut(s) 27
Bsp143I GATC 4 cut(s) 262, 376, 610, 855
BspACI CCGC 3 cut(s) 489, 620, 861
BspANI GGCC 4 cut(s) 5, 293, 488, 589
BspCNI CTCAG 2 cut(s) 813, 844
BspHI TCATGA 1 cut(s) 517
BspLI GGNNCC 2 cut(s) 292, 778
BspMAI CTGCAG 2 cut(s) 412, 841
BsrDI GCAATG 1 cut(s) 515
BssECI CCNNGG 2 cut(s) 525, 596
BssMI GATC 4 cut(s) 262, 376, 610, 855
BssT1I CCWWGG 1 cut(s) 525
Bst4CI ACNGT 2 cut(s) 86, 231
Bst6I CTCTTC 1 cut(s) 124
BstAPI GCANNNNNTGC 2 cut(s) 735, 836
BstC8I GCNNGC 3 cut(s) 391, 405, 731
BstDEI CTNAG 2 cut(s) 800, 852
BstEII GGTNACC 1 cut(s) 37
BstF5I GGATG 2 cut(s) 98, 103
BstKTI GATC 4 cut(s) 265, 379, 613, 858
BstMAI GTCTC 2 cut(s) 549, 671
BstMBI GATC 4 cut(s) 262, 376, 610, 855
BstMWI GCNNNNNNNGC 4 cut(s) 209, 735, 833, 836
BstNSI RCATGY 1 cut(s) 207
BstPI GGTNACC 1 cut(s) 37
BstSCI CCNGG 2 cut(s) 595, 596
BstSFI CTRYAG 4 cut(s) 408, 606, 658, 837
BstSLI GKGCMC 1 cut(s) 27
BstV1I GCAGC 6 cut(s) 187, 394, 422, 775, 814, 823
BstX2I RGATCY 1 cut(s) 610
BstYI RGATCY 1 cut(s) 610
BsuRI GGCC 4 cut(s) 5, 293, 488, 589
BtrI CACGTC 1 cut(s) 22
BtsCI GGATG 2 cut(s) 98, 103
BtsI GCAGTG 2 cut(s) 546, 632
BtsIMutI CAGTG 2 cut(s) 546, 632
Cac8I GCNNGC 3 cut(s) 391, 405, 731
CciI TCATGA 1 cut(s) 517
Cfr13I GGNCC 2 cut(s) 291, 587
Cfr9I CCCGGG 1 cut(s) 596
Csp6I GTAC 2 cut(s) 363, 846
CviAII CATG 5 cut(s) 204, 350, 518, 684, 691
CviQI GTAC 2 cut(s) 363, 846
DdeI CTNAG 2 cut(s) 800, 852
DpnI GATC 4 cut(s) 264, 378, 612, 857
DpnII GATC 4 cut(s) 262, 376, 610, 855
EaeI YGGCCR 1 cut(s) 486
Eam1104I CTCTTC 1 cut(s) 124
EarI CTCTTC 1 cut(s) 124
Eco130I CCWWGG 1 cut(s) 525
Eco32I GATATC 1 cut(s) 813
Eco57I CTGAAG 1 cut(s) 92
Eco88I CYCGRG 1 cut(s) 596
Eco91I GGTNACC 1 cut(s) 37
EcoO109I RGGNCCY 1 cut(s) 291
EcoO65I GGTNACC 1 cut(s) 37
EcoRV GATATC 1 cut(s) 813
EcoT14I CCWWGG 1 cut(s) 525
EcoT22I ATGCAT 1 cut(s) 121
ErhI CCWWGG 1 cut(s) 525
FaeI CATG 5 cut(s) 207, 353, 521, 687, 694
FatI CATG 5 cut(s) 203, 349, 517, 683, 690
FauNDI CATATG 1 cut(s) 115
Fnu4HI GCNGC 7 cut(s) 201, 408, 411, 489, 789, 828, 837
FokI GGATG 2 cut(s) 105, 110
Fsp4HI GCNGC 7 cut(s) 201, 408, 411, 489, 789, 828, 837
FspBI CTAG 1 cut(s) 168
GluI GCNGC 7 cut(s) 201, 408, 411, 489, 789, 828, 837
HaeIII GGCC 4 cut(s) 5, 293, 488, 589
HapII CCGG 3 cut(s) 35, 251, 597
Hin1II CATG 5 cut(s) 207, 353, 521, 687, 694
HindIII AAGCTT 2 cut(s) 320, 702
HpaII CCGG 3 cut(s) 35, 251, 597
HphI GGTGA 3 cut(s) 31, 432, 566
Hpy166II GTNNAC 1 cut(s) 25
Hpy188I TCNGA 2 cut(s) 471, 803
Hpy188III TCNNGA 2 cut(s) 518, 748
Hpy8I GTNNAC 1 cut(s) 25
Hpy99I CGWCG 1 cut(s) 23
HpyAV CCTTC 5 cut(s) 41, 200, 296, 462, 836
HpyCH4III ACNGT 2 cut(s) 86, 231
HpyCH4IV ACGT 1 cut(s) 21
HpyF10VI GCNNNNNNNGC 4 cut(s) 209, 735, 833, 836
HpyF3I CTNAG 2 cut(s) 800, 852
HpySE526I ACGT 1 cut(s) 21
Hsp92II CATG 5 cut(s) 207, 353, 521, 687, 694
Kzo9I GATC 4 cut(s) 262, 376, 610, 855
LmnI GCTCC 2 cut(s) 305, 708
Lsp1109I GCAGC 6 cut(s) 187, 394, 422, 775, 814, 823
LweI GCATC 1 cut(s) 462
MaeI CTAG 1 cut(s) 168
MaeII ACGT 1 cut(s) 21
MaeIII GTNAC 1 cut(s) 37
MalI GATC 4 cut(s) 264, 378, 612, 857
MboI GATC 4 cut(s) 262, 376, 610, 855
MboII GAAGA 1 cut(s) 141
MflI RGATCY 1 cut(s) 610
MhlI GDGCHC 1 cut(s) 27
MluCI AATT 3 cut(s) 435, 559, 715
MmeI TCCRAC 2 cut(s) 178, 640
MnlI CCTC 6 cut(s) 125, 247, 560, 568, 797, 809
Mph1103I ATGCAT 1 cut(s) 121
MroXI GAANNNNTTC 1 cut(s) 189
MseI TTAA 3 cut(s) 122, 579, 651
MslI CAYNNNNRTG 1 cut(s) 398
MspA1I CMGCKG 2 cut(s) 491, 861
MspI CCGG 3 cut(s) 35, 251, 597
MspR9I CCNGG 2 cut(s) 597, 598
MwoI GCNNNNNNNGC 4 cut(s) 209, 735, 833, 836
NciI CCSGG 2 cut(s) 597, 598
NdeI CATATG 1 cut(s) 115
NdeII GATC 4 cut(s) 262, 376, 610, 855
NlaIII CATG 5 cut(s) 207, 353, 521, 687, 694
NlaIV GGNNCC 2 cut(s) 292, 778
NmuCI GTSAC 1 cut(s) 37
NsiI ATGCAT 1 cut(s) 121
NspI RCATGY 1 cut(s) 207
PagI TCATGA 1 cut(s) 517
PdmI GAANNNNTTC 1 cut(s) 189
PkrI GCNGC 7 cut(s) 202, 409, 412, 490, 790, 829, 838
PspEI GGTNACC 1 cut(s) 37
PspN4I GGNNCC 2 cut(s) 292, 778
PspPI GGNCC 2 cut(s) 291, 587
PstI CTGCAG 2 cut(s) 412, 841
PsuI RGATCY 1 cut(s) 610
RsaI GTAC 2 cut(s) 364, 847
RsaNI GTAC 2 cut(s) 363, 846
RseI CAYNNNNRTG 1 cut(s) 398
SaqAI TTAA 3 cut(s) 122, 579, 651
SatI GCNGC 7 cut(s) 201, 408, 411, 489, 789, 828, 837
Sau3AI GATC 4 cut(s) 262, 376, 610, 855
Sau96I GGNCC 2 cut(s) 291, 587
ScrFI CCNGG 2 cut(s) 597, 598
SduI GDGCHC 1 cut(s) 27
SfaNI GCATC 1 cut(s) 462
SfcI CTRYAG 4 cut(s) 408, 606, 658, 837
SmaI CCCGGG 1 cut(s) 598
SmiMI CAYNNNNRTG 1 cut(s) 398
Sse9I AATT 3 cut(s) 435, 559, 715
SsiI CCGC 3 cut(s) 489, 620, 861
SspMI CTAG 1 cut(s) 168
StyD4I CCNGG 2 cut(s) 595, 596
StyI CCWWGG 1 cut(s) 525
TaaI ACNGT 2 cut(s) 86, 231
TaiI ACGT 1 cut(s) 24
TaqI TCGA 2 cut(s) 18, 271
TasI AATT 3 cut(s) 435, 559, 715
TatI WGTACW 1 cut(s) 362
TauI GCSGC 1 cut(s) 491
Tru1I TTAA 3 cut(s) 122, 579, 651
Tru9I TTAA 3 cut(s) 122, 579, 651
TscAI CASTG 2 cut(s) 546, 639
TseFI GTSAC 1 cut(s) 37
TseI GCWGC 6 cut(s) 200, 407, 410, 788, 827, 836
Tsp45I GTSAC 1 cut(s) 37
TspDTI ATGAA 1 cut(s) 418
TspMI CCCGGG 1 cut(s) 596
TspRI CASTG 2 cut(s) 546, 639
VneI GTGCAC 1 cut(s) 23
XapI RAATTY 2 cut(s) 559, 715
XceI RCATGY 1 cut(s) 207
XmaI CCCGGG 1 cut(s) 596
XmnI GAANNNNTTC 1 cut(s) 189
XspI CTAG 1 cut(s) 168
Zsp2I ATGCAT 1 cut(s) 121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.