Rmu_sc0002824.1_g000013

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002824.1
OG0002265
Physical Location & Seq
Reverse (-)
60482 .. 63059
2578 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002824.1_g000013.1.cds

Sequence Viewer

Length: 1059 bp
atggtggcgtgccgctcaagtcactgcgacttgttgtgcaagtttacttgcagcccacgtcgaaatacacctataaataggctgctcgacatccaggtaccccctcctcgagccgacggtcaaacttcgagtcaacactccaaggaagcttctctattgttcccggtcagctcccgctccagtccgcattcattgttgctgaatggatctatcccgagccccatcggcgacctgaacttgcctccagcagacaagacgcaaaggatgaggcaagaactcgagcaatttcggaaggaaaagcgggaccgcgaggctcgtcgtcgacgaaaactggaaaggttcctacaacttgacagtgattcgtcagacgacgacgccatcggcgatctccatcaaactaacaatgtagtagcaaatgcaggtgccagcggcgttattaggggaatctctggtggacccctcatcgagggggtgggaggcacgtcaaatggggggcagaatggcggagccggattaggccaatccaaaatggcgcgcaacaaatcgtggcgtgagaagatggaaaaaatgattgatgcatgcaagcgtgctggcccacgagagctggccgcagaaatctcaagggacgtcggtatatctccgtttaccgatgacattttgaatcctgcaaagcctcgccggagctggttcctcgagttggacagttaccttaatgcttcgggtaggatggcgtgcctcaccctagccgtgggcggcggtgggatgatggaccacaccctcgttgcagacgggggtgggatggcttacctcacccattgccgtgggcgggggtgggatggtggaacacaccctcgctgtaggcggggtaggatggcgtgcctcacccctcgccgagggtgggggtgggatgatggaccacaccctcgctgcaggcgggggtgggatggcgtgactaatttgagatgtgcaggatatggtggttatgcaatcggttctgtaagaggagtgggggcaatcccatctccggatgttgttaaagacgcaatgcgtggcggatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

352

Amino Acids

38.1

Weight (kDa)

9.62

Isoelectric Point (pI)

59.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 410
AatII GACGTC 1 cut(s) 630
Acc36I ACCTGC 1 cut(s) 410
Acc65I GGTACC 1 cut(s) 97
AccB1I GGYRCC 2 cut(s) 97, 422
AccBSI CCGCTC 2 cut(s) 15, 177
AccI GTMKAC 1 cut(s) 322
AccII CGCG 2 cut(s) 309, 535
AccIII TCCGGA 1 cut(s) 1024
AclWI GGATC 1 cut(s) 214
AcoI YGGCCR 1 cut(s) 606
AcyI GRCGYC 2 cut(s) 375, 627
AfaI GTAC 1 cut(s) 99
AfiI CCNNNNNNNGG 7 cut(s) 466, 697, 748, 826, 893, 898, 1024
AgsI TTSAA 1 cut(s) 661
AjiI CACGTC 2 cut(s) 59, 483
AjnI CCWGG 1 cut(s) 93
AluBI AGCT 4 cut(s) 149, 171, 604, 684
AluI AGCT 4 cut(s) 149, 171, 604, 684
AlwI GGATC 1 cut(s) 214
Ama87I CYCGRG 4 cut(s) 108, 214, 278, 692
Aor13HI TCCGGA 1 cut(s) 1024
AoxI GGCC 3 cut(s) 517, 592, 606
ApeKI GCWGC 3 cut(s) 51, 82, 927
ArsI GACNNNNNNTTYG 2 cut(s) 115, 147
Asp718I GGTACC 1 cut(s) 97
AspLEI GCGC 2 cut(s) 535, 537
AspS9I GGNCC 5 cut(s) 304, 455, 593, 769, 914
AsuC2I CCSGG 1 cut(s) 164
AsuHPI GGTGA 3 cut(s) 730, 802, 874
AvaI CYCGRG 4 cut(s) 108, 214, 278, 692
AvaII GGWCC 4 cut(s) 304, 455, 769, 914
BanI GGYRCC 2 cut(s) 97, 422
BanII GRGCYC 1 cut(s) 221
BauI CACGAG 1 cut(s) 597
BbvI GCAGC 3 cut(s) 63, 69, 914
BceAI ACGGC 2 cut(s) 731, 804
BciT130I CCWGG 1 cut(s) 95
BcnI CCSGG 1 cut(s) 164
BfaI CTAG 1 cut(s) 743
BfmI CTRYAG 2 cut(s) 856, 928
BfuAI ACCTGC 1 cut(s) 410
BglI GCCNNNNNGGC 1 cut(s) 225
BisI GCNGC 7 cut(s) 13, 52, 83, 430, 609, 754, 928
BlsI GCNGC 7 cut(s) 14, 53, 84, 431, 610, 755, 929
Bme1390I CCNGG 2 cut(s) 95, 164
Bme18I GGWCC 4 cut(s) 304, 455, 769, 914
BmeT110I CYCGRG 4 cut(s) 108, 214, 278, 692
BmgBI CACGTC 2 cut(s) 59, 483
BmgT120I GGNCC 5 cut(s) 304, 455, 593, 769, 914
BmiI GGNNCC 7 cut(s) 99, 305, 341, 424, 457, 508, 689
BmrFI CCNGG 2 cut(s) 95, 164
BmsI GCATC 1 cut(s) 565
BpmI CTGGAG 2 cut(s) 163, 228
BpuEI CTTGAG 1 cut(s) 604
BpuMI CCSGG 1 cut(s) 164
BsaBI GATNNNNATC 1 cut(s) 390
BsaHI GRCGYC 2 cut(s) 375, 627
BsaJI CCNNGG 4 cut(s) 141, 747, 820, 892
BsaWI WCCGGW 1 cut(s) 1024
Bsc4I CCNNNNNNNGG 7 cut(s) 466, 697, 748, 826, 893, 898, 1024
Bse1I ACTGG 2 cut(s) 180, 336
Bse3DI GCAATG 2 cut(s) 814, 1050
Bse8I GATNNNNATC 1 cut(s) 390
BseAI TCCGGA 1 cut(s) 1024
BseBI CCWGG 1 cut(s) 95
BseDI CCNNGG 4 cut(s) 141, 747, 820, 892
BseJI GATNNNNATC 1 cut(s) 390
BseLI CCNNNNNNNGG 7 cut(s) 466, 697, 748, 826, 893, 898, 1024
BseMI GCAATG 2 cut(s) 814, 1050
BseNI ACTGG 2 cut(s) 180, 336
BsePI GCGCGC 1 cut(s) 533
BseRI GAGGAG 2 cut(s) 96, 1017
BseXI GCAGC 3 cut(s) 63, 69, 914
BsgI GTGCAG 1 cut(s) 987
Bsh1236I CGCG 2 cut(s) 309, 535
BshFI GGCC 3 cut(s) 519, 594, 608
BshNI GGYRCC 2 cut(s) 97, 422
BsiHKCI CYCGRG 4 cut(s) 108, 214, 278, 692
BsiSI CCGG 4 cut(s) 164, 510, 679, 1025
BslFI GGGAC 2 cut(s) 317, 638
BslI CCNNNNNNNGG 7 cut(s) 466, 697, 748, 826, 893, 898, 1024
BsmFI GGGAC 2 cut(s) 317, 638
BsmI GAATGC 1 cut(s) 187
BsnI GGCC 3 cut(s) 519, 594, 608
BsoBI CYCGRG 4 cut(s) 108, 214, 278, 692
Bsp1286I GDGCHC 1 cut(s) 221
Bsp13I TCCGGA 1 cut(s) 1024
Bsp143I GATC 2 cut(s) 206, 385
BspANI GGCC 3 cut(s) 519, 594, 608
BspEI TCCGGA 1 cut(s) 1024
BspFNI CGCG 2 cut(s) 309, 535
BspLI GGNNCC 7 cut(s) 99, 305, 341, 424, 457, 508, 689
BspMAI CTGCAG 1 cut(s) 932
BspMI ACCTGC 1 cut(s) 410
BspPI GGATC 1 cut(s) 214
BspT107I GGYRCC 2 cut(s) 97, 422
BsrBI CCGCTC 2 cut(s) 15, 177
BsrDI GCAATG 2 cut(s) 814, 1050
BsrI ACTGG 2 cut(s) 180, 336
BssECI CCNNGG 4 cut(s) 141, 747, 820, 892
BssHII GCGCGC 1 cut(s) 533
BssMI GATC 2 cut(s) 206, 385
BssNI GRCGYC 2 cut(s) 375, 627
BssSI CACGAG 1 cut(s) 597
BssT1I CCWWGG 1 cut(s) 141
Bst2BI CACGAG 1 cut(s) 597
Bst2UI CCWGG 1 cut(s) 95
Bst4CI ACNGT 3 cut(s) 119, 356, 704
BstACI GRCGYC 2 cut(s) 375, 627
BstDSI CCRYGG 2 cut(s) 747, 820
BstENI CCTNNNNNAGG 2 cut(s) 464, 891
BstFNI CGCG 2 cut(s) 309, 535
BstHHI GCGC 2 cut(s) 535, 537
BstKTI GATC 2 cut(s) 209, 388
BstMBI GATC 2 cut(s) 206, 385
BstMWI GCNNNNNNNGC 1 cut(s) 225
BstNI CCWGG 1 cut(s) 95
BstNSI RCATGY 1 cut(s) 582
BstSCI CCNGG 2 cut(s) 93, 162
BstSFI CTRYAG 2 cut(s) 856, 928
BstUI CGCG 2 cut(s) 309, 535
BstV1I GCAGC 3 cut(s) 63, 69, 914
BstX2I RGATCY 1 cut(s) 206
BstXI CCANNNNNNTGG 1 cut(s) 821
BstYI RGATCY 1 cut(s) 206
BsuRI GGCC 3 cut(s) 519, 594, 608
BtgI CCRYGG 2 cut(s) 747, 820
BtrI CACGTC 2 cut(s) 59, 483
BtsI GCAGTG 1 cut(s) 22
BtsIMutI CAGTG 2 cut(s) 22, 361
BveI ACCTGC 1 cut(s) 410
CfoI GCGC 2 cut(s) 535, 537
Cfr13I GGNCC 5 cut(s) 304, 455, 593, 769, 914
CseI GACGC 3 cut(s) 265, 383, 1049
Csp6I GTAC 1 cut(s) 98
CviAII CATG 1 cut(s) 579
CviQI GTAC 1 cut(s) 98
DpnI GATC 2 cut(s) 208, 387
DpnII GATC 2 cut(s) 206, 385
EaeI YGGCCR 1 cut(s) 606
EciI GGCGGA 1 cut(s) 519
Eco130I CCWWGG 1 cut(s) 141
Eco24I GRGCYC 1 cut(s) 221
Eco47I GGWCC 4 cut(s) 304, 455, 769, 914
Eco88I CYCGRG 4 cut(s) 108, 214, 278, 692
EcoNI CCTNNNNNAGG 2 cut(s) 464, 891
EcoRII CCWGG 1 cut(s) 93
EcoT14I CCWWGG 1 cut(s) 141
EcoT22I ATGCAT 1 cut(s) 580
EcoT38I GRGCYC 1 cut(s) 221
ErhI CCWWGG 1 cut(s) 141
FaeI CATG 1 cut(s) 582
FaiI YATR 5 cut(s) 74, 580, 635, 975, 984
FaqI GGGAC 2 cut(s) 317, 638
FatI CATG 1 cut(s) 578
FauI CCCGC 5 cut(s) 182, 294, 819, 855, 927
FblI GTMKAC 1 cut(s) 322
Fnu4HI GCNGC 7 cut(s) 13, 52, 83, 430, 609, 754, 928
FriOI GRGCYC 1 cut(s) 221
Fsp4HI GCNGC 7 cut(s) 13, 52, 83, 430, 609, 754, 928
FspBI CTAG 1 cut(s) 743
GlaI GCGC 2 cut(s) 534, 536
GluI GCNGC 7 cut(s) 13, 52, 83, 430, 609, 754, 928
GsuI CTGGAG 2 cut(s) 163, 228
HaeIII GGCC 3 cut(s) 519, 594, 608
HapII CCGG 4 cut(s) 164, 510, 679, 1025
HgaI GACGC 3 cut(s) 265, 383, 1049
HhaI GCGC 2 cut(s) 535, 537
Hin1I GRCGYC 2 cut(s) 375, 627
Hin1II CATG 1 cut(s) 582
Hin6I GCGC 2 cut(s) 533, 535
HinP1I GCGC 2 cut(s) 533, 535
HincII GTYRAC 2 cut(s) 134, 323
HindII GTYRAC 2 cut(s) 134, 323
HindIII AAGCTT 1 cut(s) 147
HinfI GANTC 4 cut(s) 130, 359, 444, 661
HpaII CCGG 4 cut(s) 164, 510, 679, 1025
HphI GGTGA 3 cut(s) 730, 802, 874
Hpy166II GTNNAC 5 cut(s) 45, 134, 323, 455, 645
Hpy188I TCNGA 2 cut(s) 291, 367
Hpy188III TCNNGA 2 cut(s) 214, 1025
Hpy8I GTNNAC 5 cut(s) 45, 134, 323, 455, 645
Hpy99I CGWCG 8 cut(s) 63, 119, 321, 324, 327, 374, 377, 632
HpyAV CCTTC 1 cut(s) 286
HpyCH4III ACNGT 3 cut(s) 119, 356, 704
HpyCH4IV ACGT 3 cut(s) 58, 482, 627
HpyF10VI GCNNNNNNNGC 1 cut(s) 225
HpySE526I ACGT 3 cut(s) 58, 482, 627
Hsp92I GRCGYC 2 cut(s) 375, 627
Hsp92II CATG 1 cut(s) 582
HspAI GCGC 2 cut(s) 533, 535
Kpn2I TCCGGA 1 cut(s) 1024
KpnI GGTACC 1 cut(s) 101
Kzo9I GATC 2 cut(s) 206, 385
LmnI GCTCC 4 cut(s) 176, 182, 506, 681
Lsp1109I GCAGC 3 cut(s) 63, 69, 914
LweI GCATC 1 cut(s) 565
MaeI CTAG 1 cut(s) 743
MaeII ACGT 3 cut(s) 58, 482, 627
MaeIII GTNAC 3 cut(s) 20, 704, 949
MalI GATC 2 cut(s) 208, 387
MbiI CCGCTC 2 cut(s) 15, 177
MboI GATC 2 cut(s) 206, 385
MboII GAAGA 1 cut(s) 568
MflI RGATCY 1 cut(s) 206
MhlI GDGCHC 1 cut(s) 221
MluCI AATT 2 cut(s) 284, 955
MlyI GAGTC 1 cut(s) 139
MmeI TCCRAC 1 cut(s) 678
Mph1103I ATGCAT 1 cut(s) 580
MroI TCCGGA 1 cut(s) 1024
MseI TTAA 2 cut(s) 711, 1035
MslI CAYNNNNRTG 1 cut(s) 819
MspA1I CMGCKG 1 cut(s) 429
MspI CCGG 4 cut(s) 164, 510, 679, 1025
MspR9I CCNGG 2 cut(s) 95, 164
Mva1269I GAATGC 1 cut(s) 187
MvaI CCWGG 1 cut(s) 95
MvnI CGCG 2 cut(s) 309, 535
MwoI GCNNNNNNNGC 1 cut(s) 225
NciI CCSGG 1 cut(s) 164
NdeII GATC 2 cut(s) 206, 385
NlaIII CATG 1 cut(s) 582
NlaIV GGNNCC 7 cut(s) 99, 305, 341, 424, 457, 508, 689
NmeAIII GCCGAG 1 cut(s) 917
NmuCI GTSAC 2 cut(s) 20, 949
NsiI ATGCAT 1 cut(s) 580
NspI RCATGY 1 cut(s) 582
PaeI GCATGC 1 cut(s) 582
PaeR7I CTCGAG 3 cut(s) 108, 278, 692
PaqCI CACCTGC 1 cut(s) 410
PauI GCGCGC 1 cut(s) 533
PcsI WCGNNNNNNNCGW 2 cut(s) 322, 381
PctI GAATGC 1 cut(s) 187
PfeI GAWTC 3 cut(s) 359, 444, 661
PkrI GCNGC 7 cut(s) 14, 53, 84, 431, 610, 755, 929
PleI GAGTC 1 cut(s) 138
PpsI GAGTC 1 cut(s) 138
Psp6I CCWGG 1 cut(s) 93
PspGI CCWGG 1 cut(s) 93
PspN4I GGNNCC 7 cut(s) 99, 305, 341, 424, 457, 508, 689
PspPI GGNCC 5 cut(s) 304, 455, 593, 769, 914
PspXI VCTCGAGB 3 cut(s) 108, 278, 692
PstI CTGCAG 1 cut(s) 932
PsuI RGATCY 1 cut(s) 206
PteI GCGCGC 1 cut(s) 533
RsaI GTAC 1 cut(s) 99
RsaNI GTAC 1 cut(s) 98
RseI CAYNNNNRTG 1 cut(s) 819
SalI GTCGAC 1 cut(s) 321
SaqAI TTAA 2 cut(s) 711, 1035
SatI GCNGC 7 cut(s) 13, 52, 83, 430, 609, 754, 928
Sau3AI GATC 2 cut(s) 206, 385
Sau96I GGNCC 5 cut(s) 304, 455, 593, 769, 914
SchI GAGTC 1 cut(s) 139
ScrFI CCNGG 2 cut(s) 95, 164
SduI GDGCHC 1 cut(s) 221
SfaNI GCATC 1 cut(s) 565
SfcI CTRYAG 2 cut(s) 856, 928
Sfr274I CTCGAG 3 cut(s) 108, 278, 692
SgrDI CGTCGACG 1 cut(s) 321
SinI GGWCC 4 cut(s) 304, 455, 769, 914
SlaI CTCGAG 3 cut(s) 108, 278, 692
SmiMI CAYNNNNRTG 1 cut(s) 819
SmlI CTYRAG 5 cut(s) 16, 108, 278, 619, 692
SmoI CTYRAG 5 cut(s) 16, 108, 278, 619, 692
SphI GCATGC 1 cut(s) 582
Sse9I AATT 2 cut(s) 284, 955
SspMI CTAG 1 cut(s) 743
StyD4I CCNGG 2 cut(s) 93, 162
StyI CCWWGG 1 cut(s) 141
TaaI ACNGT 3 cut(s) 119, 356, 704
TaiI ACGT 3 cut(s) 61, 485, 630
TaqI TCGA 8 cut(s) 61, 87, 109, 128, 279, 322, 465, 693
TasI AATT 2 cut(s) 284, 955
TauI GCSGC 4 cut(s) 15, 432, 611, 756
TfiI GAWTC 3 cut(s) 359, 444, 661
Tru1I TTAA 2 cut(s) 711, 1035
Tru9I TTAA 2 cut(s) 711, 1035
TscAI CASTG 2 cut(s) 29, 361
TseFI GTSAC 2 cut(s) 20, 949
TseI GCWGC 3 cut(s) 51, 82, 927
Tsp45I GTSAC 2 cut(s) 20, 949
TspDTI ATGAA 1 cut(s) 180
TspGWI ACGGA 1 cut(s) 630
TspRI CASTG 2 cut(s) 29, 361
VpaK11BI GGWCC 4 cut(s) 304, 455, 769, 914
XagI CCTNNNNNAGG 2 cut(s) 464, 891
XceI RCATGY 1 cut(s) 582
XhoI CTCGAG 3 cut(s) 108, 278, 692
XmiI GTMKAC 1 cut(s) 322
XspI CTAG 1 cut(s) 743
ZraI GACGTC 1 cut(s) 628
Zsp2I ATGCAT 1 cut(s) 580
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.