Rroxscaffold_3G00225780

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0002265
Physical Location & Seq
Reverse (-)
9045579 .. 9046744
1166 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00225780.1

Sequence Viewer

Length: 954 bp
ATGGCGGGAGCTACCCCCGACGGGATTCCATTTACGTCACCGAGGATGGTAGTGACGAAATTCCGCTCCACCGACCACCTAGAAAAGAGGGACCGCGAGGCCCGTCGTCTACGAAAACTTGAAAGGTTCCCGCAACTTGACGGTGATTCTTCGGATGACAACGCCATCGGCGATCTCCATCAAGCCAACAACATTGTAGCCAATGCGGAGCCGGCGGCGTTATTGGGGGAATATTCGCGCGGACCCCTCGTCGAGGGGGTGGGAGGCACGTTGAATGGGGGGCGAACGACGGAGCCGGGAATAGGCCAATACAAAAGGGCGCGCAACGAATTGTGGCGCGAGAAGATGGAAAAAATGATTGATGCATGCAAGCGTGCCGACCCACAAGAGTTGGCGGCGGAAATCGCAAGGGACGTCGGCAAGTCTCGTTTGCGACGACATCCTAAATGCGGCAAAGCCCCGCCGGTTTACCACGCCGTATTCCATAAGTATGATGGAACGATCGATCCGGTAGACCATATCAAGGGATACAAAACAACGGATGTCCATCGAGACAACCGACGAAAAATTAATGTGCAAGATTTTCCCTTCCAATCTCACGAGGCCGGCGTCGACATGGTTTCAAGATCCCAAGCCTCATTCTATACCGGATTTCGACACATTGAGCCGTGCTTTCATTTCACAATACTTCGCAATCGCAAGCAAAAGAAGGATATGGCGACTCGTTCGGTACCAAGCAAAAGCCGGAGAAAAGGATCCGCCGAAGGACATGGACGATATCATACAAGGGTTGAGGGAGAGATCATAATTGAAAGAGCCAAAGAGGCACGCGAGGTTCGATACACCGTGTCGTCGCGTCCGGTGATGAAAGAAGGCAAAATTGAGGTAGCAGCCGCCGGCGTGAGGGCGACAACCGACATCACTTTGAAGCAAAGCCTCGAGCTCGACTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

317

Amino Acids

35.8

Weight (kDa)

9.68

Isoelectric Point (pI)

59.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 417
Acc65I GGTACC 1 cut(s) 730
AccB1I GGYRCC 1 cut(s) 730
AccBSI CCGCTC 1 cut(s) 66
AccI GTMKAC 3 cut(s) 109, 513, 612
AccII CGCG 7 cut(s) 96, 238, 240, 322, 339, 831, 856
AclWI GGATC 4 cut(s) 500, 621, 750, 763
AcsI RAATTY 1 cut(s) 59
AcyI GRCGYC 2 cut(s) 414, 609
AfaI GTAC 1 cut(s) 732
AfiI CCNNNNNNNGG 6 cut(s) 21, 253, 302, 449, 523, 903
AgsI TTSAA 5 cut(s) 122, 274, 624, 812, 928
AhdI GACNNNNNGTC 1 cut(s) 248
AloI GAACNNNNNNTCC 2 cut(s) 490, 522
AluBI AGCT 2 cut(s) 11, 943
AluI AGCT 2 cut(s) 11, 943
Alw21I GWGCWC 1 cut(s) 945
Alw26I GTCTC 2 cut(s) 429, 546
AlwI GGATC 4 cut(s) 500, 621, 750, 763
Ama87I CYCGRG 1 cut(s) 938
AoxI GGCC 3 cut(s) 99, 304, 603
ApeKI GCWGC 1 cut(s) 890
ApoI RAATTY 1 cut(s) 59
AseI ATTAAT 1 cut(s) 570
Asp718I GGTACC 1 cut(s) 730
AspLEI GCGC 4 cut(s) 240, 322, 324, 339
AspS9I GGNCC 3 cut(s) 91, 100, 242
AsuC2I CCSGG 1 cut(s) 297
AsuHPI GGTGA 3 cut(s) 30, 155, 874
AvaI CYCGRG 1 cut(s) 938
AvaII GGWCC 2 cut(s) 91, 242
BamHI GGATCC 1 cut(s) 755
BanI GGYRCC 1 cut(s) 730
BanII GRGCYC 1 cut(s) 945
BauI CACGAG 1 cut(s) 599
Bbv12I GWGCWC 1 cut(s) 945
BbvI GCAGC 1 cut(s) 902
BccI CCATC 6 cut(s) 40, 173, 186, 340, 488, 555
BceAI ACGGC 2 cut(s) 461, 652
BciVI GTATCC 1 cut(s) 521
BcnI CCSGG 1 cut(s) 297
BcoDI GTCTC 2 cut(s) 429, 546
BfaI CTAG 1 cut(s) 80
BfuI GTATCC 1 cut(s) 521
BglI GCCNNNNNGGC 1 cut(s) 824
BisI GCNGC 5 cut(s) 216, 396, 451, 891, 894
BlsI GCNGC 5 cut(s) 217, 397, 452, 892, 895
Bme1390I CCNGG 1 cut(s) 297
Bme18I GGWCC 2 cut(s) 91, 242
BmeRI GACNNNNNGTC 1 cut(s) 248
BmeT110I CYCGRG 1 cut(s) 938
BmgT120I GGNCC 3 cut(s) 91, 100, 242
BmiI GGNNCC 7 cut(s) 92, 128, 210, 244, 294, 732, 757
BmrFI CCNGG 1 cut(s) 297
BmsI GCATC 1 cut(s) 352
BpuMI CCSGG 1 cut(s) 297
Bsa29I ATCGAT 1 cut(s) 504
BsaBI GATNNNNATC 2 cut(s) 177, 546
BsaHI GRCGYC 2 cut(s) 414, 609
BsaJI CCNNGG 1 cut(s) 41
BsaWI WCCGGW 3 cut(s) 508, 647, 859
Bsc4I CCNNNNNNNGG 6 cut(s) 21, 253, 302, 449, 523, 903
Bse118I RCCGGY 4 cut(s) 211, 463, 605, 896
Bse8I GATNNNNATC 2 cut(s) 177, 546
BseCI ATCGAT 1 cut(s) 504
BseDI CCNNGG 1 cut(s) 41
BseGI GGATG 4 cut(s) 51, 160, 439, 547
BseJI GATNNNNATC 2 cut(s) 177, 546
BseLI CCNNNNNNNGG 6 cut(s) 21, 253, 302, 449, 523, 903
BsePI GCGCGC 1 cut(s) 320
BseXI GCAGC 1 cut(s) 902
Bsh1236I CGCG 7 cut(s) 96, 238, 240, 322, 339, 831, 856
Bsh1285I CGRYCG 1 cut(s) 504
BshFI GGCC 3 cut(s) 101, 306, 605
BshNI GGYRCC 1 cut(s) 730
BshVI ATCGAT 1 cut(s) 504
BsiEI CGRYCG 1 cut(s) 504
BsiHKAI GWGCWC 1 cut(s) 945
BsiHKCI CYCGRG 1 cut(s) 938
BsiSI CCGG 9 cut(s) 212, 296, 464, 509, 606, 648, 745, 860, 897
BslFI GGGAC 2 cut(s) 104, 425
BslI CCNNNNNNNGG 6 cut(s) 21, 253, 302, 449, 523, 903
BsmAI GTCTC 2 cut(s) 429, 546
BsmFI GGGAC 2 cut(s) 104, 425
BsnI GGCC 3 cut(s) 101, 306, 605
BsoBI CYCGRG 1 cut(s) 938
Bsp1286I GDGCHC 1 cut(s) 945
Bsp143I GATC 6 cut(s) 172, 501, 505, 626, 755, 801
BspANI GGCC 3 cut(s) 101, 306, 605
BspDI ATCGAT 1 cut(s) 504
BspFNI CGCG 7 cut(s) 96, 238, 240, 322, 339, 831, 856
BspLI GGNNCC 7 cut(s) 92, 128, 210, 244, 294, 732, 757
BspPI GGATC 4 cut(s) 500, 621, 750, 763
BspT107I GGYRCC 1 cut(s) 730
BsrBI CCGCTC 1 cut(s) 66
BsrFI RCCGGY 4 cut(s) 211, 463, 605, 896
BssAI RCCGGY 4 cut(s) 211, 463, 605, 896
BssECI CCNNGG 1 cut(s) 41
BssHII GCGCGC 1 cut(s) 320
BssMI GATC 6 cut(s) 172, 501, 505, 626, 755, 801
BssNI GRCGYC 2 cut(s) 414, 609
BssSI CACGAG 1 cut(s) 599
Bst2BI CACGAG 1 cut(s) 599
Bst4CI ACNGT 2 cut(s) 143, 847
BstACI GRCGYC 2 cut(s) 414, 609
BstC8I GCNNGC 9 cut(s) 213, 322, 367, 371, 375, 607, 701, 829, 898
BstENI CCTNNNNNAGG 1 cut(s) 251
BstF5I GGATG 4 cut(s) 51, 160, 439, 547
BstFNI CGCG 7 cut(s) 96, 238, 240, 322, 339, 831, 856
BstHHI GCGC 4 cut(s) 240, 322, 324, 339
BstKTI GATC 6 cut(s) 175, 504, 508, 629, 758, 804
BstMAI GTCTC 2 cut(s) 429, 546
BstMBI GATC 6 cut(s) 172, 501, 505, 626, 755, 801
BstMCI CGRYCG 1 cut(s) 504
BstMWI GCNNNNNNNGC 3 cut(s) 212, 404, 824
BstNSI RCATGY 1 cut(s) 369
BstSCI CCNGG 1 cut(s) 295
BstUI CGCG 7 cut(s) 96, 238, 240, 322, 339, 831, 856
BstV1I GCAGC 1 cut(s) 902
BstX2I RGATCY 2 cut(s) 626, 755
BstYI RGATCY 2 cut(s) 626, 755
Bsu15I ATCGAT 1 cut(s) 504
BsuI GTATCC 1 cut(s) 521
BsuRI GGCC 3 cut(s) 101, 306, 605
BsuTUI ATCGAT 1 cut(s) 504
BtsCI GGATG 4 cut(s) 51, 160, 439, 547
Cac8I GCNNGC 9 cut(s) 213, 322, 367, 371, 375, 607, 701, 829, 898
CfoI GCGC 4 cut(s) 240, 322, 324, 339
Cfr10I RCCGGY 4 cut(s) 211, 463, 605, 896
Cfr13I GGNCC 3 cut(s) 91, 100, 242
ClaI ATCGAT 1 cut(s) 504
CseI GACGC 2 cut(s) 598, 845
Csp6I GTAC 1 cut(s) 731
CspCI CAANNNNNGTGG 2 cut(s) 372, 407
CviAII CATG 3 cut(s) 366, 616, 770
CviQI GTAC 1 cut(s) 731
DpnI GATC 6 cut(s) 174, 503, 507, 628, 757, 803
DpnII GATC 6 cut(s) 172, 501, 505, 626, 755, 801
DriI GACNNNNNGTC 1 cut(s) 248
Eam1105I GACNNNNNGTC 1 cut(s) 248
EciI GGCGGA 2 cut(s) 413, 748
Ecl136II GAGCTC 1 cut(s) 943
Eco24I GRGCYC 1 cut(s) 945
Eco32I GATATC 1 cut(s) 779
Eco47I GGWCC 2 cut(s) 91, 242
Eco53kI GAGCTC 1 cut(s) 943
Eco88I CYCGRG 1 cut(s) 938
EcoICRI GAGCTC 1 cut(s) 943
EcoNI CCTNNNNNAGG 1 cut(s) 251
EcoRV GATATC 1 cut(s) 779
EcoT22I ATGCAT 1 cut(s) 367
EcoT38I GRGCYC 1 cut(s) 945
FaeI CATG 3 cut(s) 369, 619, 773
FaqI GGGAC 2 cut(s) 104, 425
FatI CATG 3 cut(s) 365, 615, 769
FauI CCCGC 2 cut(s) 138, 468
FblI GTMKAC 3 cut(s) 109, 513, 612
Fnu4HI GCNGC 5 cut(s) 216, 396, 451, 891, 894
FokI GGATG 4 cut(s) 58, 167, 426, 554
FriOI GRGCYC 1 cut(s) 945
Fsp4HI GCNGC 5 cut(s) 216, 396, 451, 891, 894
FspBI CTAG 1 cut(s) 80
GlaI GCGC 4 cut(s) 239, 321, 323, 338
GluI GCNGC 5 cut(s) 216, 396, 451, 891, 894
HaeIII GGCC 3 cut(s) 101, 306, 605
HapII CCGG 9 cut(s) 212, 296, 464, 509, 606, 648, 745, 860, 897
HgaI GACGC 2 cut(s) 598, 845
HhaI GCGC 4 cut(s) 240, 322, 324, 339
Hin1I GRCGYC 2 cut(s) 414, 609
Hin1II CATG 3 cut(s) 369, 619, 773
Hin6I GCGC 4 cut(s) 238, 320, 322, 337
HinP1I GCGC 4 cut(s) 238, 320, 322, 337
HincII GTYRAC 1 cut(s) 613
HindII GTYRAC 1 cut(s) 613
HinfI GANTC 3 cut(s) 25, 146, 721
HpaII CCGG 9 cut(s) 212, 296, 464, 509, 606, 648, 745, 860, 897
HphI GGTGA 3 cut(s) 30, 155, 874
Hpy166II GTNNAC 4 cut(s) 110, 469, 514, 613
Hpy188I TCNGA 1 cut(s) 154
Hpy188III TCNNGA 3 cut(s) 551, 599, 624
Hpy8I GTNNAC 4 cut(s) 110, 469, 514, 613
Hpy99I CGWCG 9 cut(s) 23, 108, 254, 292, 419, 438, 564, 614, 856
HpyAV CCTTC 4 cut(s) 598, 703, 758, 866
HpyCH4III ACNGT 2 cut(s) 143, 847
HpyCH4IV ACGT 3 cut(s) 35, 269, 414
HpyCH4V TGCA 3 cut(s) 365, 369, 577
HpyF10VI GCNNNNNNNGC 3 cut(s) 212, 404, 824
HpySE526I ACGT 3 cut(s) 35, 269, 414
Hsp92I GRCGYC 2 cut(s) 414, 609
Hsp92II CATG 3 cut(s) 369, 619, 773
HspAI GCGC 4 cut(s) 238, 320, 322, 337
KpnI GGTACC 1 cut(s) 734
KroI GCCGGC 3 cut(s) 211, 605, 896
KroNI GCCGGC 3 cut(s) 213, 607, 898
Kzo9I GATC 6 cut(s) 172, 501, 505, 626, 755, 801
LmnI GCTCC 4 cut(s) 8, 71, 208, 292
LpnPI CCDG 9 cut(s) 225, 309, 477, 522, 619, 661, 758, 873, 910
Lsp1109I GCAGC 1 cut(s) 902
LweI GCATC 1 cut(s) 352
MaeI CTAG 1 cut(s) 80
MaeII ACGT 3 cut(s) 35, 269, 414
MaeIII GTNAC 2 cut(s) 36, 52
MalI GATC 6 cut(s) 174, 503, 507, 628, 757, 803
MbiI CCGCTC 1 cut(s) 66
MboI GATC 6 cut(s) 172, 501, 505, 626, 755, 801
MboII GAAGA 2 cut(s) 141, 355
MflI RGATCY 2 cut(s) 626, 755
MhlI GDGCHC 1 cut(s) 945
MluCI AATT 5 cut(s) 59, 329, 567, 807, 879
MlyI GAGTC 1 cut(s) 715
Mph1103I ATGCAT 1 cut(s) 367
MreI CGCCGGCG 1 cut(s) 896
MroNI GCCGGC 3 cut(s) 211, 605, 896
MseI TTAA 1 cut(s) 570
MslI CAYNNNNRTG 1 cut(s) 489
MspI CCGG 9 cut(s) 212, 296, 464, 509, 606, 648, 745, 860, 897
MspR9I CCNGG 1 cut(s) 297
MvnI CGCG 7 cut(s) 96, 238, 240, 322, 339, 831, 856
MwoI GCNNNNNNNGC 3 cut(s) 212, 404, 824
NaeI GCCGGC 3 cut(s) 213, 607, 898
NciI CCSGG 1 cut(s) 297
NdeII GATC 6 cut(s) 172, 501, 505, 626, 755, 801
NgoMIV GCCGGC 3 cut(s) 211, 605, 896
NlaIII CATG 3 cut(s) 369, 619, 773
NlaIV GGNNCC 7 cut(s) 92, 128, 210, 244, 294, 732, 757
NmuCI GTSAC 2 cut(s) 36, 52
NsiI ATGCAT 1 cut(s) 367
NspI RCATGY 1 cut(s) 369
PaeI GCATGC 1 cut(s) 369
PaeR7I CTCGAG 1 cut(s) 938
PauI GCGCGC 1 cut(s) 320
PcsI WCGNNNNNNNCGW 6 cut(s) 168, 411, 433, 556, 606, 835
PdiI GCCGGC 3 cut(s) 213, 607, 898
PfeI GAWTC 2 cut(s) 25, 146
PkrI GCNGC 5 cut(s) 217, 397, 452, 892, 895
Ple19I CGATCG 1 cut(s) 504
PleI GAGTC 1 cut(s) 715
PpsI GAGTC 1 cut(s) 715
PshBI ATTAAT 1 cut(s) 570
Psp124BI GAGCTC 1 cut(s) 945
PspN4I GGNNCC 7 cut(s) 92, 128, 210, 244, 294, 732, 757
PspPI GGNCC 3 cut(s) 91, 100, 242
PspXI VCTCGAGB 1 cut(s) 938
PsuI RGATCY 2 cut(s) 626, 755
PteI GCGCGC 1 cut(s) 320
PvuI CGATCG 1 cut(s) 504
RsaI GTAC 1 cut(s) 732
RsaNI GTAC 1 cut(s) 731
RseI CAYNNNNRTG 1 cut(s) 489
SacI GAGCTC 1 cut(s) 945
SalI GTCGAC 1 cut(s) 611
SaqAI TTAA 1 cut(s) 570
SatI GCNGC 5 cut(s) 216, 396, 451, 891, 894
Sau3AI GATC 6 cut(s) 172, 501, 505, 626, 755, 801
Sau96I GGNCC 3 cut(s) 91, 100, 242
SchI GAGTC 1 cut(s) 715
ScrFI CCNGG 1 cut(s) 297
SduI GDGCHC 1 cut(s) 945
SetI ASST 9 cut(s) 13, 38, 81, 128, 272, 417, 837, 888, 945
SfaNI GCATC 1 cut(s) 352
Sfr274I CTCGAG 1 cut(s) 938
SgrAI CRCCGGYG 1 cut(s) 896
SinI GGWCC 2 cut(s) 91, 242
SlaI CTCGAG 1 cut(s) 938
SmiMI CAYNNNNRTG 1 cut(s) 489
SmlI CTYRAG 1 cut(s) 938
SmoI CTYRAG 1 cut(s) 938
SphI GCATGC 1 cut(s) 369
Sse9I AATT 5 cut(s) 59, 329, 567, 807, 879
SspI AATATT 1 cut(s) 233
SspMI CTAG 1 cut(s) 80
SstI GAGCTC 1 cut(s) 945
StyD4I CCNGG 1 cut(s) 295
TaaI ACNGT 2 cut(s) 143, 847
TaiI ACGT 3 cut(s) 38, 272, 417
TaqI TCGA 8 cut(s) 252, 504, 550, 612, 655, 838, 939, 945
TasI AATT 5 cut(s) 59, 329, 567, 807, 879
TauI GCSGC 4 cut(s) 218, 398, 453, 896
TfiI GAWTC 2 cut(s) 25, 146
Tru1I TTAA 1 cut(s) 570
Tru9I TTAA 1 cut(s) 570
TseFI GTSAC 2 cut(s) 36, 52
TseI GCWGC 1 cut(s) 890
Tsp45I GTSAC 2 cut(s) 36, 52
TspDTI ATGAA 2 cut(s) 665, 881
TspGWI ACGGA 2 cut(s) 305, 554
VpaK11BI GGWCC 2 cut(s) 91, 242
VspI ATTAAT 1 cut(s) 570
XagI CCTNNNNNAGG 1 cut(s) 251
XapI RAATTY 1 cut(s) 59
XceI RCATGY 1 cut(s) 369
XcmI CCANNNNNNNNNTGG 1 cut(s) 491
XhoI CTCGAG 1 cut(s) 938
XmiI GTMKAC 3 cut(s) 109, 513, 612
XspI CTAG 1 cut(s) 80
ZraI GACGTC 1 cut(s) 415
Zsp2I ATGCAT 1 cut(s) 367
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.