Rw3G025420

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0002265
Physical Location & Seq
Forward (+)
33924626 .. 33925204
579 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G025420.1

Sequence Viewer

Length: 579 bp
ATGGCCGACCGCTGCCACCCGAGACCAGATCCCGTCGCAACAATCGGCACCAGCCCTCGCCACTGCCACTGTCTCCAGCCTCGCCGCCACACTGCAAGACCCGACCTCGCTACCTCAACCCACGTCGAAACCAGTCGAAAGCAGCCCGATTCAGCATGTCGCCGCCACCGTCCAGCCCCTCCACGACGCTGCCGGTCATGGAGATCCAGGAGGTGCCTCAACGACACTCGATCCGCGTTGCACCACCGCCCAATGAGTCCAAGATTTCTTTCGGTGCTCGTCTCTAAAACCATCCAGCGCCGGACTCGAGCCGTAATCCCTCTCCAAGAAACTGCCGGGCCACTAGTTTTCAAATTCCCATCCGGCAGTCACGTCAAGCAGGCGAGGCAAGCCAACGCCGCCCAAGCAGGCCGCCCCTTTCTGTCGCTAAGGACTGGGAGCGACGAAGTCCAACCCCCTGACCAGCCCCTGAATGAGCCTCTTCCAAACTTGGTCGGCGATCTAAACTTGCCTCTAGTCGGCGAGGCACAAACGCTGAGACAAGAACTTGAGCAACTCCGGAGGGAAAAGCAAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

192

Amino Acids

21.74

Weight (kDa)

11.26

Isoelectric Point (pI)

82.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 47, 213
AccII CGCG 1 cut(s) 236
AccIII TCCGGA 1 cut(s) 558
AciI CCGC 7 cut(s) 10, 85, 163, 234, 247, 399, 412
AclWI GGATC 3 cut(s) 23, 198, 225
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 353
AfiI CCNNNNNNNGG 1 cut(s) 518
AgsI TTSAA 1 cut(s) 352
AhlI ACTAGT 1 cut(s) 343
AjiI CACGTC 2 cut(s) 124, 373
AjnI CCWGG 1 cut(s) 206
AjuI GAANNNNNNNTTGG 2 cut(s) 253, 285
Alw21I GWGCWC 1 cut(s) 279
Alw26I GTCTC 4 cut(s) 16, 77, 286, 532
AlwI GGATC 3 cut(s) 23, 198, 225
AlwNI CAGNNNCTG 1 cut(s) 469
Ama87I CYCGRG 2 cut(s) 19, 306
Aor13HI TCCGGA 1 cut(s) 558
AoxI GGCC 3 cut(s) 3, 338, 409
ApeKI GCWGC 3 cut(s) 12, 142, 189
ApoI RAATTY 1 cut(s) 353
AspLEI GCGC 1 cut(s) 300
AspS9I GGNCC 1 cut(s) 338
AsuC2I CCSGG 1 cut(s) 337
AvaI CYCGRG 2 cut(s) 19, 306
BanI GGYRCC 2 cut(s) 47, 213
Bbv12I GWGCWC 1 cut(s) 279
BbvI GCAGC 2 cut(s) 154, 176
BccI CCATC 2 cut(s) 299, 367
BceAI ACGGC 1 cut(s) 296
BciT130I CCWGG 1 cut(s) 208
BcnI CCSGG 1 cut(s) 337
BcoDI GTCTC 4 cut(s) 16, 77, 286, 532
BcuI ACTAGT 1 cut(s) 343
BfaI CTAG 2 cut(s) 344, 515
BfoI RGCGCY 1 cut(s) 301
BisI GCNGC 7 cut(s) 13, 85, 143, 163, 190, 399, 412
BlsI GCNGC 7 cut(s) 14, 86, 144, 164, 191, 400, 413
Bme1390I CCNGG 2 cut(s) 208, 337
BmeT110I CYCGRG 2 cut(s) 19, 306
BmgBI CACGTC 2 cut(s) 124, 373
BmgT120I GGNCC 1 cut(s) 338
BmiI GGNNCC 2 cut(s) 49, 215
BmrFI CCNGG 2 cut(s) 208, 337
BmrI ACTGGG 1 cut(s) 444
BmuI ACTGGG 1 cut(s) 444
BpmI CTGGAG 1 cut(s) 59
Bpu10I CCTNAGC 1 cut(s) 428
BpuEI CTTGAG 1 cut(s) 569
BpuMI CCSGG 1 cut(s) 337
BsaI GGTCTC 1 cut(s) 16
BsaWI WCCGGW 1 cut(s) 558
Bsc4I CCNNNNNNNGG 1 cut(s) 518
Bse118I RCCGGY 1 cut(s) 192
Bse1I ACTGG 2 cut(s) 132, 439
BseAI TCCGGA 1 cut(s) 558
BseBI CCWGG 1 cut(s) 208
BseGI GGATG 2 cut(s) 291, 359
BseLI CCNNNNNNNGG 1 cut(s) 518
BseMII CTCAG 1 cut(s) 527
BseNI ACTGG 2 cut(s) 132, 439
BseXI GCAGC 2 cut(s) 154, 176
Bsh1236I CGCG 1 cut(s) 236
Bsh1285I CGRYCG 1 cut(s) 10
BshFI GGCC 3 cut(s) 5, 340, 411
BshNI GGYRCC 2 cut(s) 47, 213
BsiEI CGRYCG 1 cut(s) 10
BsiHKAI GWGCWC 1 cut(s) 279
BsiHKCI CYCGRG 2 cut(s) 19, 306
BsiSI CCGG 5 cut(s) 193, 301, 336, 363, 559
BslI CCNNNNNNNGG 1 cut(s) 518
BsmAI GTCTC 4 cut(s) 16, 77, 286, 532
BsmBI CGTCTC 1 cut(s) 286
BsnI GGCC 3 cut(s) 5, 340, 411
Bso31I GGTCTC 1 cut(s) 16
BsoBI CYCGRG 2 cut(s) 19, 306
Bsp1286I GDGCHC 1 cut(s) 279
Bsp13I TCCGGA 1 cut(s) 558
Bsp143I GATC 4 cut(s) 28, 203, 230, 499
BspACI CCGC 7 cut(s) 10, 85, 163, 234, 247, 399, 412
BspANI GGCC 3 cut(s) 5, 340, 411
BspCNI CTCAG 1 cut(s) 528
BspEI TCCGGA 1 cut(s) 558
BspFNI CGCG 1 cut(s) 236
BspLI GGNNCC 2 cut(s) 49, 215
BspPI GGATC 3 cut(s) 23, 198, 225
BspT107I GGYRCC 2 cut(s) 47, 213
BspTNI GGTCTC 1 cut(s) 16
BsrFI RCCGGY 1 cut(s) 192
BsrI ACTGG 2 cut(s) 132, 439
BssAI RCCGGY 1 cut(s) 192
BssMI GATC 4 cut(s) 28, 203, 230, 499
Bst2UI CCWGG 1 cut(s) 208
Bst4CI ACNGT 2 cut(s) 71, 170
Bst6I CTCTTC 1 cut(s) 486
BstC8I GCNNGC 3 cut(s) 381, 390, 409
BstDEI CTNAG 2 cut(s) 428, 536
BstF5I GGATG 2 cut(s) 291, 359
BstFNI CGCG 1 cut(s) 236
BstH2I RGCGCY 1 cut(s) 301
BstHHI GCGC 1 cut(s) 300
BstKTI GATC 4 cut(s) 31, 206, 233, 502
BstMAI GTCTC 4 cut(s) 16, 77, 286, 532
BstMBI GATC 4 cut(s) 28, 203, 230, 499
BstMCI CGRYCG 1 cut(s) 10
BstMWI GCNNNNNNNGC 4 cut(s) 385, 389, 398, 404
BstNI CCWGG 1 cut(s) 208
BstNSI RCATGY 1 cut(s) 159
BstSCI CCNGG 2 cut(s) 206, 335
BstUI CGCG 1 cut(s) 236
BstV1I GCAGC 2 cut(s) 154, 176
BstX2I RGATCY 2 cut(s) 28, 203
BstYI RGATCY 2 cut(s) 28, 203
BsuRI GGCC 3 cut(s) 5, 340, 411
BtrI CACGTC 2 cut(s) 124, 373
BtsCI GGATG 2 cut(s) 291, 359
BtsI GCAGTG 2 cut(s) 61, 90
BtsIMutI CAGTG 3 cut(s) 61, 67, 90
Cac8I GCNNGC 3 cut(s) 381, 390, 409
CaiI CAGNNNCTG 1 cut(s) 469
CfoI GCGC 1 cut(s) 300
Cfr10I RCCGGY 1 cut(s) 192
Cfr13I GGNCC 1 cut(s) 338
CseI GACGC 1 cut(s) 195
CviAII CATG 2 cut(s) 156, 198
DdeI CTNAG 2 cut(s) 428, 536
DpnI GATC 4 cut(s) 30, 205, 232, 501
DpnII GATC 4 cut(s) 28, 203, 230, 499
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 486
EarI CTCTTC 1 cut(s) 486
Eco31I GGTCTC 1 cut(s) 16
Eco88I CYCGRG 2 cut(s) 19, 306
EcoRII CCWGG 1 cut(s) 206
Esp3I CGTCTC 1 cut(s) 286
FaeI CATG 2 cut(s) 159, 201
FaiI YATR 2 cut(s) 157, 199
FatI CATG 2 cut(s) 155, 197
Fnu4HI GCNGC 7 cut(s) 13, 85, 143, 163, 190, 399, 412
FokI GGATG 2 cut(s) 278, 346
Fsp4HI GCNGC 7 cut(s) 13, 85, 143, 163, 190, 399, 412
FspBI CTAG 2 cut(s) 344, 515
GlaI GCGC 1 cut(s) 299
GluI GCNGC 7 cut(s) 13, 85, 143, 163, 190, 399, 412
GsuI CTGGAG 1 cut(s) 59
HaeII RGCGCY 1 cut(s) 301
HaeIII GGCC 3 cut(s) 5, 340, 411
HapII CCGG 5 cut(s) 193, 301, 336, 363, 559
HgaI GACGC 1 cut(s) 195
HhaI GCGC 1 cut(s) 300
Hin1II CATG 2 cut(s) 159, 201
Hin6I GCGC 1 cut(s) 298
HinP1I GCGC 1 cut(s) 298
HinfI GANTC 3 cut(s) 149, 256, 304
HpaII CCGG 5 cut(s) 193, 301, 336, 363, 559
Hpy188III TCNNGA 1 cut(s) 559
Hpy99I CGWCG 4 cut(s) 38, 128, 189, 446
HpyCH4III ACNGT 2 cut(s) 71, 170
HpyCH4IV ACGT 2 cut(s) 123, 372
HpyCH4V TGCA 2 cut(s) 95, 241
HpyF10VI GCNNNNNNNGC 4 cut(s) 385, 389, 398, 404
HpyF3I CTNAG 2 cut(s) 428, 536
HpySE526I ACGT 2 cut(s) 123, 372
Hsp92II CATG 2 cut(s) 159, 201
HspAI GCGC 1 cut(s) 298
Kpn2I TCCGGA 1 cut(s) 558
Kzo9I GATC 4 cut(s) 28, 203, 230, 499
LmnI GCTCC 1 cut(s) 438
Lsp1109I GCAGC 2 cut(s) 154, 176
MaeI CTAG 2 cut(s) 344, 515
MaeII ACGT 2 cut(s) 123, 372
MaeIII GTNAC 1 cut(s) 368
MalI GATC 4 cut(s) 30, 205, 232, 501
MboI GATC 4 cut(s) 28, 203, 230, 499
MboII GAAGA 1 cut(s) 473
MflI RGATCY 2 cut(s) 28, 203
MhlI GDGCHC 1 cut(s) 279
MluCI AATT 1 cut(s) 353
MlyI GAGTC 2 cut(s) 265, 298
MmeI TCCRAC 1 cut(s) 475
MroI TCCGGA 1 cut(s) 558
MspA1I CMGCKG 1 cut(s) 12
MspI CCGG 5 cut(s) 193, 301, 336, 363, 559
MspR9I CCNGG 2 cut(s) 208, 337
MvaI CCWGG 1 cut(s) 208
MvnI CGCG 1 cut(s) 236
MwoI GCNNNNNNNGC 4 cut(s) 385, 389, 398, 404
NciI CCSGG 1 cut(s) 337
NdeII GATC 4 cut(s) 28, 203, 230, 499
NlaIII CATG 2 cut(s) 159, 201
NlaIV GGNNCC 2 cut(s) 49, 215
NmuCI GTSAC 1 cut(s) 368
NspI RCATGY 1 cut(s) 159
PaeR7I CTCGAG 1 cut(s) 306
PcsI WCGNNNNNNNCGW 1 cut(s) 166
PfeI GAWTC 1 cut(s) 149
PflFI GACNNNGTC 1 cut(s) 446
PfoI TCCNGGA 1 cut(s) 206
PkrI GCNGC 7 cut(s) 14, 86, 144, 164, 191, 400, 413
PleI GAGTC 2 cut(s) 264, 298
PpsI GAGTC 2 cut(s) 264, 298
Psp6I CCWGG 1 cut(s) 206
PspGI CCWGG 1 cut(s) 206
PspN4I GGNNCC 2 cut(s) 49, 215
PspPI GGNCC 1 cut(s) 338
PspXI VCTCGAGB 1 cut(s) 306
PstNI CAGNNNCTG 1 cut(s) 469
PsuI RGATCY 2 cut(s) 28, 203
PsyI GACNNNGTC 1 cut(s) 446
SatI GCNGC 7 cut(s) 13, 85, 143, 163, 190, 399, 412
Sau3AI GATC 4 cut(s) 28, 203, 230, 499
Sau96I GGNCC 1 cut(s) 338
SchI GAGTC 2 cut(s) 265, 298
ScrFI CCNGG 2 cut(s) 208, 337
SduI GDGCHC 1 cut(s) 279
SetI ASST 5 cut(s) 108, 116, 126, 215, 375
Sfr274I CTCGAG 1 cut(s) 306
SlaI CTCGAG 1 cut(s) 306
SmlI CTYRAG 2 cut(s) 306, 548
SmoI CTYRAG 2 cut(s) 306, 548
SpeI ACTAGT 1 cut(s) 343
Sse9I AATT 1 cut(s) 353
SsiI CCGC 7 cut(s) 10, 85, 163, 234, 247, 399, 412
SspMI CTAG 2 cut(s) 344, 515
StyD4I CCNGG 2 cut(s) 206, 335
TaaI ACNGT 2 cut(s) 71, 170
TaiI ACGT 2 cut(s) 126, 375
TaqI TCGA 4 cut(s) 126, 136, 229, 307
TasI AATT 1 cut(s) 353
TauI GCSGC 4 cut(s) 87, 165, 401, 414
TfiI GAWTC 1 cut(s) 149
TscAI CASTG 3 cut(s) 68, 74, 97
TseFI GTSAC 1 cut(s) 368
TseI GCWGC 3 cut(s) 12, 142, 189
Tsp45I GTSAC 1 cut(s) 368
TspRI CASTG 3 cut(s) 68, 74, 97
Tth111I GACNNNGTC 1 cut(s) 446
XapI RAATTY 1 cut(s) 353
XceI RCATGY 1 cut(s) 159
XhoI CTCGAG 1 cut(s) 306
XspI CTAG 2 cut(s) 344, 515
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.