Rmu_sc0005543.1_g000001

EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005543.1
OG0001544
Physical Location & Seq
Forward (+)
1 .. 3255
3255 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1025 bp
cggacgggatcaccagcagcggtgaagataccaacttcaccgaggacctcaccgatgctggggctgaaatccaaagcagagtatgtggtggctctggaaacgacaagtcgtctagcactaaaccccactgccgtcgatcgcaaagccgccgcggcctcagaacccgattcgccgtcgctgatgacgtcttgagtttttccgatccacaccatgagtttcatcttcgcaaacagaaaacacccgcaggtaaaataggaggcattgctgtggttgatttggatcgagagaggaaagggatggagtctaggatggatcagtatgagctgatggagcagatcggccggggagctttcggagccgcgattttggttcatcacaaggctgagaagaagaagtatgtgttgaagaagatccggcttgcgaggcagacggagcggtgccggagatctgctcatcaagaggcatatgcattaaatggaagaggattagttgggggaagtttccaacttgattaccctagtgttggggcaacagaaacccttctaatggctgcatgtatggctgatggaacaactatactgtccaatgttgccagagaaccagaggtggttgatcttgctcgatttctgactaaaagtggggcctgtgtggaaggagcaggaagcttgacatcaagggaaaatctcatttgcatggtttcagcagtgtctcgatgtggtgagaatttgcgaggttttgaggttaagacagggccatttcccgggtttcctacagatctccaaccgcaaacaatggaactgctaaccacttggtttaagtcctgtagaagtctctatttgaaaagcgcatggctcatgtctgtgctctgctctggtttcgggaaagataatagaaatggtttgtctggttcctgccttgctgcaactgacctcagaggtgggatatcattggtattagctgcattggctgcagaaggtactactgagatcagcggtgttgctcatattgtggttatgagaatgtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPSP_synthase

Protein Analysis

340

Amino Acids

36.76

Weight (kDa)

8.78

Isoelectric Point (pI)

43.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPSP_synthase PF00275 159 - 221 1.8e-11 EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 188
Acc36I ACCTGC 1 cut(s) 235
AccB1I GGYRCC 1 cut(s) 437
AccBSI CCGCTC 1 cut(s) 435
AccII CGCG 2 cut(s) 152, 361
AciI CCGC 9 cut(s) 20, 147, 150, 152, 242, 359, 435, 784, 992
AclWI GGATC 5 cut(s) 16, 196, 287, 320, 405
AcoI YGGCCR 1 cut(s) 339
AcsI RAATTY 1 cut(s) 723
AcyI GRCGYC 1 cut(s) 185
AfaI GTAC 1 cut(s) 978
AfiI CCNNNNNNNGG 4 cut(s) 59, 523, 546, 760
AgsI TTSAA 2 cut(s) 405, 840
AhdI GACNNNNNGTC 1 cut(s) 108
AluBI AGCT 4 cut(s) 324, 349, 665, 958
AluI AGCT 4 cut(s) 324, 349, 665, 958
Alw21I GWGCWC 1 cut(s) 866
Alw26I GTCTC 2 cut(s) 713, 835
AlwI GGATC 5 cut(s) 16, 196, 287, 320, 405
Ama87I CYCGRG 1 cut(s) 760
AoxI GGCC 4 cut(s) 153, 339, 641, 751
ApeKI GCWGC 5 cut(s) 17, 550, 919, 958, 967
ApoI RAATTY 1 cut(s) 723
Asp700I GAANNNNTTC 1 cut(s) 539
AspLEI GCGC 1 cut(s) 847
AspS9I GGNCC 3 cut(s) 45, 641, 751
AsuC2I CCSGG 3 cut(s) 343, 761, 762
AsuHPI GGTGA 5 cut(s) 3, 30, 34, 42, 730
AvaI CYCGRG 1 cut(s) 760
AvaII GGWCC 1 cut(s) 45
BanI GGYRCC 1 cut(s) 437
Bbv12I GWGCWC 1 cut(s) 866
BbvI GCAGC 5 cut(s) 29, 537, 906, 945, 954
BccI CCATC 4 cut(s) 291, 303, 321, 559
BceAI ACGGC 2 cut(s) 116, 157
BcnI CCSGG 3 cut(s) 343, 761, 762
BcoDI GTCTC 2 cut(s) 713, 835
BfaI CTAG 3 cut(s) 113, 305, 518
BfmI CTRYAG 3 cut(s) 770, 822, 968
BfuAI ACCTGC 1 cut(s) 235
BglI GCCNNNNNGGC 1 cut(s) 152
BglII AGATCT 2 cut(s) 445, 774
BisI GCNGC 9 cut(s) 18, 147, 150, 153, 359, 551, 920, 959, 968
BlsI GCNGC 9 cut(s) 19, 148, 151, 154, 360, 552, 921, 960, 969
Bme1390I CCNGG 3 cut(s) 343, 761, 762
Bme18I GGWCC 1 cut(s) 45
BmeRI GACNNNNNGTC 1 cut(s) 108
BmeT110I CYCGRG 1 cut(s) 760
BmgT120I GGNCC 3 cut(s) 45, 641, 751
BmiI GGNNCC 4 cut(s) 357, 439, 642, 909
BmrFI CCNGG 3 cut(s) 343, 761, 762
BmsI GCATC 1 cut(s) 45
BplI GAGNNNNNCTC 2 cut(s) 435, 467
BpuEI CTTGAG 1 cut(s) 210
BpuMI CCSGG 3 cut(s) 343, 761, 762
BsaBI GATNNNNATC 1 cut(s) 278
BsaHI GRCGYC 1 cut(s) 185
BsaJI CCNNGG 4 cut(s) 41, 150, 342, 760
Bsc4I CCNNNNNNNGG 4 cut(s) 59, 523, 546, 760
Bse3DI GCAATG 1 cut(s) 260
Bse8I GATNNNNATC 1 cut(s) 278
BseDI CCNNGG 4 cut(s) 41, 150, 342, 760
BseGI GGATG 2 cut(s) 302, 314
BseJI GATNNNNATC 1 cut(s) 278
BseLI CCNNNNNNNGG 4 cut(s) 59, 523, 546, 760
BseMI GCAATG 1 cut(s) 260
BseMII CTCAG 4 cut(s) 171, 374, 945, 974
BseX3I CGGCCG 1 cut(s) 339
BseXI GCAGC 5 cut(s) 29, 537, 906, 945, 954
BseYI CCCAGC 1 cut(s) 58
Bsh1236I CGCG 2 cut(s) 152, 361
Bsh1285I CGRYCG 2 cut(s) 139, 342
BshFI GGCC 4 cut(s) 155, 341, 643, 753
BshNI GGYRCC 1 cut(s) 437
BsiEI CGRYCG 2 cut(s) 139, 342
BsiHKAI GWGCWC 1 cut(s) 866
BsiHKCI CYCGRG 1 cut(s) 760
BsiSI CCGG 4 cut(s) 342, 414, 441, 761
BslI CCNNNNNNNGG 4 cut(s) 59, 523, 546, 760
BsmAI GTCTC 2 cut(s) 713, 835
BsnI GGCC 4 cut(s) 155, 341, 643, 753
BsoBI CYCGRG 1 cut(s) 760
Bsp1286I GDGCHC 1 cut(s) 866
BspACI CCGC 9 cut(s) 20, 147, 150, 152, 242, 359, 435, 784, 992
BspANI GGCC 4 cut(s) 155, 341, 643, 753
BspCNI CTCAG 4 cut(s) 170, 375, 944, 975
BspFNI CGCG 2 cut(s) 152, 361
BspLI GGNNCC 4 cut(s) 357, 439, 642, 909
BspMAI CTGCAG 1 cut(s) 972
BspMI ACCTGC 1 cut(s) 235
BspPI GGATC 5 cut(s) 16, 196, 287, 320, 405
BspT107I GGYRCC 1 cut(s) 437
BsrBI CCGCTC 1 cut(s) 435
BsrDI GCAATG 1 cut(s) 260
BssECI CCNNGG 4 cut(s) 41, 150, 342, 760
BssNI GRCGYC 1 cut(s) 185
Bst4CI ACNGT 1 cut(s) 581
Bst6I CTCTTC 1 cut(s) 474
BstACI GRCGYC 1 cut(s) 185
BstAPI GCANNNNNTGC 1 cut(s) 967
BstC8I GCNNGC 1 cut(s) 419
BstDEI CTNAG 4 cut(s) 157, 383, 931, 983
BstDSI CCRYGG 1 cut(s) 150
BstF5I GGATG 2 cut(s) 302, 314
BstFNI CGCG 2 cut(s) 152, 361
BstHHI GCGC 1 cut(s) 847
BstMAI GTCTC 2 cut(s) 713, 835
BstMCI CGRYCG 2 cut(s) 139, 342
BstMWI GCNNNNNNNGC 8 cut(s) 152, 330, 355, 423, 432, 559, 964, 967
BstNSI RCATGY 1 cut(s) 557
BstSCI CCNGG 3 cut(s) 341, 759, 760
BstSFI CTRYAG 3 cut(s) 770, 822, 968
BstUI CGCG 2 cut(s) 152, 361
BstV1I GCAGC 5 cut(s) 29, 537, 906, 945, 954
BstX2I RGATCY 3 cut(s) 410, 445, 774
BstYI RGATCY 3 cut(s) 410, 445, 774
BstZI CGGCCG 1 cut(s) 339
BsuRI GGCC 4 cut(s) 155, 341, 643, 753
BtgI CCRYGG 1 cut(s) 150
BtsCI GGATG 2 cut(s) 302, 314
BtsI GCAGTG 2 cut(s) 126, 710
BtsIMutI CAGTG 2 cut(s) 126, 710
BveI ACCTGC 1 cut(s) 235
Cac8I GCNNGC 1 cut(s) 419
CfoI GCGC 1 cut(s) 847
Cfr13I GGNCC 3 cut(s) 45, 641, 751
Cfr42I CCGCGG 1 cut(s) 153
Cfr9I CCCGGG 1 cut(s) 760
Csp6I GTAC 1 cut(s) 977
CviAII CATG 5 cut(s) 211, 554, 693, 848, 855
CviQI GTAC 1 cut(s) 977
DdeI CTNAG 4 cut(s) 157, 383, 931, 983
DriI GACNNNNNGTC 1 cut(s) 108
EaeI YGGCCR 1 cut(s) 339
EagI CGGCCG 1 cut(s) 339
Eam1104I CTCTTC 1 cut(s) 474
Eam1105I GACNNNNNGTC 1 cut(s) 108
EarI CTCTTC 1 cut(s) 474
EclXI CGGCCG 1 cut(s) 339
Eco32I GATATC 1 cut(s) 944
Eco47I GGWCC 1 cut(s) 45
Eco52I CGGCCG 1 cut(s) 339
Eco88I CYCGRG 1 cut(s) 760
EcoO109I RGGNCCY 2 cut(s) 45, 641
EcoRV GATATC 1 cut(s) 944
EcoT22I ATGCAT 1 cut(s) 471
FaeI CATG 5 cut(s) 214, 557, 696, 851, 858
FatI CATG 5 cut(s) 210, 553, 692, 847, 854
FauI CCCGC 1 cut(s) 249
FauNDI CATATG 1 cut(s) 465
Fnu4HI GCNGC 9 cut(s) 18, 147, 150, 153, 359, 551, 920, 959, 968
FokI GGATG 2 cut(s) 309, 321
Fsp4HI GCNGC 9 cut(s) 18, 147, 150, 153, 359, 551, 920, 959, 968
FspBI CTAG 3 cut(s) 113, 305, 518
GlaI GCGC 1 cut(s) 846
GluI GCNGC 9 cut(s) 18, 147, 150, 153, 359, 551, 920, 959, 968
GsaI CCCAGC 1 cut(s) 62
HaeIII GGCC 4 cut(s) 155, 341, 643, 753
HapII CCGG 4 cut(s) 342, 414, 441, 761
HhaI GCGC 1 cut(s) 847
Hin1I GRCGYC 1 cut(s) 185
Hin1II CATG 5 cut(s) 214, 557, 696, 851, 858
Hin6I GCGC 1 cut(s) 845
HinP1I GCGC 1 cut(s) 845
HindIII AAGCTT 1 cut(s) 663
HinfI GANTC 2 cut(s) 167, 301
HpaII CCGG 4 cut(s) 342, 414, 441, 761
HphI GGTGA 5 cut(s) 3, 30, 34, 42, 730
Hpy188I TCNGA 5 cut(s) 160, 201, 355, 629, 934
Hpy188III TCNNGA 6 cut(s) 95, 189, 283, 457, 710, 879
Hpy99I CGWCG 2 cut(s) 137, 178
HpyAV CCTTC 3 cut(s) 550, 646, 967
HpyCH4III ACNGT 1 cut(s) 581
HpyCH4IV ACGT 1 cut(s) 185
HpyCH4V TGCA 6 cut(s) 469, 553, 692, 922, 961, 970
HpyF10VI GCNNNNNNNGC 8 cut(s) 152, 330, 355, 423, 432, 559, 964, 967
HpyF3I CTNAG 4 cut(s) 157, 383, 931, 983
HpySE526I ACGT 1 cut(s) 185
Hsp92I GRCGYC 1 cut(s) 185
Hsp92II CATG 5 cut(s) 214, 557, 696, 851, 858
HspAI GCGC 1 cut(s) 845
KspI CCGCGG 1 cut(s) 153
LmnI GCTCC 5 cut(s) 330, 346, 355, 432, 655
Lsp1109I GCAGC 5 cut(s) 29, 537, 906, 945, 954
LweI GCATC 1 cut(s) 45
MaeI CTAG 3 cut(s) 113, 305, 518
MaeII ACGT 1 cut(s) 185
MbiI CCGCTC 1 cut(s) 435
MboII GAAGA 7 cut(s) 37, 214, 399, 402, 417, 420, 491
MflI RGATCY 3 cut(s) 410, 445, 774
MhlI GDGCHC 1 cut(s) 866
MluCI AATT 1 cut(s) 723
MlyI GAGTC 1 cut(s) 310
MmeI TCCRAC 2 cut(s) 528, 804
Mph1103I ATGCAT 1 cut(s) 471
MroXI GAANNNNTTC 1 cut(s) 539
MseI TTAA 3 cut(s) 472, 743, 815
MslI CAYNNNNRTG 3 cut(s) 265, 691, 859
MspA1I CMGCKG 3 cut(s) 20, 152, 992
MspI CCGG 4 cut(s) 342, 414, 441, 761
MspR9I CCNGG 3 cut(s) 343, 761, 762
MvnI CGCG 2 cut(s) 152, 361
MwoI GCNNNNNNNGC 8 cut(s) 152, 330, 355, 423, 432, 559, 964, 967
NciI CCSGG 3 cut(s) 343, 761, 762
NdeI CATATG 1 cut(s) 465
NlaIII CATG 5 cut(s) 214, 557, 696, 851, 858
NlaIV GGNNCC 4 cut(s) 357, 439, 642, 909
NsiI ATGCAT 1 cut(s) 471
NspI RCATGY 1 cut(s) 557
PcsI WCGNNNNNNNCGW 1 cut(s) 182
PdmI GAANNNNTTC 1 cut(s) 539
PfeI GAWTC 1 cut(s) 167
PkrI GCNGC 9 cut(s) 19, 148, 151, 154, 360, 552, 921, 960, 969
Ple19I CGATCG 1 cut(s) 139
PleI GAGTC 1 cut(s) 309
PpsI GAGTC 1 cut(s) 309
PpuMI RGGWCCY 1 cut(s) 45
Psp5II RGGWCCY 1 cut(s) 45
PspFI CCCAGC 1 cut(s) 58
PspN4I GGNNCC 4 cut(s) 357, 439, 642, 909
PspPI GGNCC 3 cut(s) 45, 641, 751
PspPPI RGGWCCY 1 cut(s) 45
PstI CTGCAG 1 cut(s) 972
PsuI RGATCY 3 cut(s) 410, 445, 774
PvuI CGATCG 1 cut(s) 139
RsaI GTAC 1 cut(s) 978
RsaNI GTAC 1 cut(s) 977
RseI CAYNNNNRTG 3 cut(s) 265, 691, 859
SacII CCGCGG 1 cut(s) 153
SaqAI TTAA 3 cut(s) 472, 743, 815
SatI GCNGC 9 cut(s) 18, 147, 150, 153, 359, 551, 920, 959, 968
Sau96I GGNCC 3 cut(s) 45, 641, 751
SchI GAGTC 1 cut(s) 310
ScrFI CCNGG 3 cut(s) 343, 761, 762
SduI GDGCHC 1 cut(s) 866
SfaNI GCATC 1 cut(s) 45
SfcI CTRYAG 3 cut(s) 770, 822, 968
Sfr303I CCGCGG 1 cut(s) 153
SgrBI CCGCGG 1 cut(s) 153
SinI GGWCC 1 cut(s) 45
SmaI CCCGGG 1 cut(s) 762
SmiMI CAYNNNNRTG 3 cut(s) 265, 691, 859
SmlI CTYRAG 1 cut(s) 189
SmoI CTYRAG 1 cut(s) 189
Sse9I AATT 1 cut(s) 723
SsiI CCGC 9 cut(s) 20, 147, 150, 152, 242, 359, 435, 784, 992
SspMI CTAG 3 cut(s) 113, 305, 518
StyD4I CCNGG 3 cut(s) 341, 759, 760
TaaI ACNGT 1 cut(s) 581
TaiI ACGT 1 cut(s) 188
TaqI TCGA 4 cut(s) 135, 282, 621, 711
TasI AATT 1 cut(s) 723
TauI GCSGC 4 cut(s) 149, 152, 155, 361
TfiI GAWTC 1 cut(s) 167
Tru1I TTAA 3 cut(s) 472, 743, 815
Tru9I TTAA 3 cut(s) 472, 743, 815
TscAI CASTG 2 cut(s) 133, 710
TseI GCWGC 5 cut(s) 17, 550, 919, 958, 967
TspDTI ATGAA 2 cut(s) 208, 361
TspGWI ACGGA 1 cut(s) 445
TspMI CCCGGG 1 cut(s) 760
TspRI CASTG 2 cut(s) 133, 710
VpaK11BI GGWCC 1 cut(s) 45
XapI RAATTY 1 cut(s) 723
XceI RCATGY 1 cut(s) 557
XmaI CCCGGG 1 cut(s) 760
XmnI GAANNNNTTC 1 cut(s) 539
XspI CTAG 3 cut(s) 113, 305, 518
ZraI GACGTC 1 cut(s) 186
Zsp2I ATGCAT 1 cut(s) 471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.