Rh4DG338800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0002409
Physical Location & Seq
Forward (+)
56126589 .. 56129874
3286 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG338800.1

Sequence Viewer

Length: 1986 bp
ATGCAGGATAGTAATATATCTCTGATATTAATATCAGATTCTATGTCAACGGGCGACGCCATTAATCTAGTGAAGAAGAACGAAATACAAGATGAAGAAGTGTGTACGTTTATGTTAACGCCAACGCAATTCATTGAAACCACTCAGGGTCTGACAATGGACCAAGCAAATGTCGTCTCCAGTATCGGCTTCGGGGAGTTACATCACCTGGGATGCAGCACAATATTTGACACGGTATGTGAGATGATGCTTAATAGCATTGATCCTCTAAATGAGATTGTGTTGATACATGGCAAGGAAATACCCTTTGGACCCGCAGATTTTGCACGTGTCATGGGCATAAAGGATGGTGGATTAGACATTCAGAACATGGGAGATACGAACGAAAGTCTTGTTAGGTTAATTAGGAAACAATTGGGGGGTGGAGAAGGTGGACTACACATTGCAAGTTTGAAGAAATGTATCATGGAAAGTACACGGGCTGATGAAATATTCAAGATAGCTTATGCACTTTATGCGGTCGCATGCATTTTATGTCCCAACGGAACCAATTTGGTCGACCAGTCATTGATTTCTACTCTAGTAGAGACGAACTTGATAAAGGATCTGAATTGGGCAACGTTCTGCTACTATAAGATGCAGGAATCAGTCATATCGATGCAATCGAAAGGGTTCCGCAACCAAGCAATTTGTGTGCTTTTCCTACAAAACTTTTACTTTGACGTCGTTGATAATGAATCCAGATGGGTTGACAAAGGAATAACTCCAATCGCTGCATGGAACACTACTTTAGTGAAGAAATTGATTAGGTGGGTGGAACAAAATGGGGGAGTCACCAACACAAACCAGAATGTTTGGAGGAGACAACCTATAATCCATGAGAAGGAGTCTGATGCAAATGGGAAAATATATGATGCAAAACAAATATTGTTGAGTGTAGAAGGCCTGCATAAAAGGGTTTCTAATATGGAACAAAAAGAAAGTCGGCTGACAGAATCAATGGAGAGAATGGAGAGGATGCTAGGCTCATTCCAAGGGGCAACAATTAAACATATTGTTGGAGAGGTGTTGGCTCGTTTAGAACAGCGAGATGAAGGCAAGCATGGGGAGTGCCAAGATCAATTTAAGTTCAGAGCGGACCTTAATGTGCATGAATTCAGAGGCTCAGAGGCACAGTCTAATGATCCATCAAGACTATTGAAAGGCAAAAATATTTGCATGAACCCCCTGCCAGACGAGGTTTGCATGGGTAGAAAGATGAATCCCCCGCTCAAAGTCCAATCAAAATGTTCATTTAGCCCAGATGGTCCTCCGAAGCCGGCTAAGCGATCAATAAAGGAAAAGAAACTAAGGGGTGTCGGGGTGCAGTCAATGCATATACGGAAGGCTCTTTTCAGAACACGAGGAAGAACATATGGCAAGCAAACAAGAGTGGAACTGAAGGATGATGAAAAACAAGAGGAACAAGGTCTTCGTTGCCTATTCAACGTCAAAGCAAATACTCCAAAGGAGGACATTGAGTTGCTGACTTTCATTGGGTTTAGTAAGAAGCCACTTACAAATCTTCAAATGAGGTCATCAACATGTTTGCAAGCTACCTTAATGAAGATGGGTTTGGGGATTGGTACTTCCCAACCTATTTTGGCAAGCTCTAATGTGAAAAATATTCAGATGACCAGAATGGAATGGATTGAACACACCTGTCGCGTTAGCATGTTGGATAGATTCAAGGGGAAATTCAGGATGTGCAGAAGGATATACATTCCTTTGCGCGATCCTGCATCTGAGGGGCATTGGTATTTGTTGGTGGTGCACAAAGACTCCAGATTTGGGGAAATCGTTGACAGTGCACCTGATGATGATAGGACTTCATTCCGTGAGGAAGTTGCAAGAATGGCGGTAATACTAAACAATAACGTTTATGTTAACTATTGTCATATTGAATTGAGCTGTCAGCAACCACATATCTTAATCAGTCAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMG_box

Protein Analysis

661

Amino Acids

74.89

Weight (kDa)

8.3

Isoelectric Point (pI)

47.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 726
AccBSI CCGCTC 2 cut(s) 1136, 1270
AccI GTMKAC 1 cut(s) 558
AccII CGCG 2 cut(s) 1707, 1773
AciI CCGC 6 cut(s) 315, 518, 676, 1136, 1268, 1898
AclI AACGTT 2 cut(s) 620, 1917
AclWI GGATC 4 cut(s) 257, 612, 1178, 1769
AcsI RAATTY 2 cut(s) 1154, 1736
AcuI CTGAAG 1 cut(s) 1460
AcvI CACGTG 1 cut(s) 329
AcyI GRCGYC 2 cut(s) 57, 723
AfaI GTAC 3 cut(s) 106, 475, 1627
AfiI CCNNNNNNNGG 2 cut(s) 883, 1830
AflIII ACRYGT 2 cut(s) 328, 1583
AgsI TTSAA 9 cut(s) 137, 454, 496, 1201, 1486, 1568, 1694, 1729, 1943
AjnI CCWGG 1 cut(s) 207
AjuI GAANNNNNNNTTGG 4 cut(s) 291, 323, 1598, 1630
AluBI AGCT 4 cut(s) 503, 1595, 1650, 1950
AluI AGCT 4 cut(s) 503, 1595, 1650, 1950
Alw21I GWGCWC 2 cut(s) 1815, 1852
Alw26I GTCTC 3 cut(s) 181, 581, 856
Alw44I GTGCAC 2 cut(s) 1811, 1848
AlwI GGATC 4 cut(s) 257, 612, 1178, 1769
AlwNI CAGNNNCTG 1 cut(s) 151
AoxI GGCC 1 cut(s) 943
ApaLI GTGCAC 2 cut(s) 1811, 1848
ApeKI GCWGC 2 cut(s) 216, 773
ApoI RAATTY 2 cut(s) 1154, 1736
ArsI GACNNNNNNTTYG 4 cut(s) 967, 999, 1811, 1843
AseI ATTAAT 2 cut(s) 29, 63
Asp700I GAANNNNTTC 1 cut(s) 671
AspLEI GCGC 1 cut(s) 1773
AspS9I GGNCC 4 cut(s) 160, 311, 1138, 1307
AsuHPI GGTGA 2 cut(s) 197, 826
AvaII GGWCC 4 cut(s) 160, 311, 1138, 1307
BaeGI GKGCMC 2 cut(s) 1815, 1852
BarI GAAGNNNNNNTAC 2 cut(s) 420, 452
BauI CACGAG 1 cut(s) 1401
BbrPI CACGTG 1 cut(s) 329
BbsI GAAGAC 1 cut(s) 1463
Bbv12I GWGCWC 2 cut(s) 1815, 1852
BbvI GCAGC 2 cut(s) 228, 760
BccI CCATC 5 cut(s) 341, 738, 1195, 1298, 1603
BciT130I CCWGG 1 cut(s) 209
BcoDI GTCTC 3 cut(s) 181, 581, 856
BfaI CTAG 3 cut(s) 68, 581, 1022
BisI GCNGC 2 cut(s) 217, 774
BlpI GCTNAGC 1 cut(s) 1323
BlsI GCNGC 2 cut(s) 218, 775
Bme1390I CCNGG 1 cut(s) 209
Bme18I GGWCC 4 cut(s) 160, 311, 1138, 1307
BmgT120I GGNCC 4 cut(s) 160, 311, 1138, 1307
BmiI GGNNCC 3 cut(s) 313, 547, 674
BmrFI CCNGG 1 cut(s) 209
BmsI GCATC 8 cut(s) 203, 237, 627, 648, 883, 904, 1008, 1790
BpiI GAAGAC 1 cut(s) 1463
BpmI CTGGAG 2 cut(s) 163, 1807
Bpu1102I GCTNAGC 1 cut(s) 1323
Bsa29I ATCGAT 1 cut(s) 656
BsaAI YACGTR 1 cut(s) 329
BsaHI GRCGYC 2 cut(s) 57, 723
BsaJI CCNNGG 2 cut(s) 208, 1033
BsaXI ACNNNNNCTCC 2 cut(s) 1805, 1835
Bsc4I CCNNNNNNNGG 2 cut(s) 883, 1830
Bse118I RCCGGY 1 cut(s) 1318
Bse1I ACTGG 2 cut(s) 180, 562
Bse3DI GCAATG 1 cut(s) 441
BseBI CCWGG 1 cut(s) 209
BseCI ATCGAT 1 cut(s) 656
BseDI CCNNGG 2 cut(s) 208, 1033
BseGI GGATG 5 cut(s) 218, 352, 1023, 1450, 1749
BseLI CCNNNNNNNGG 2 cut(s) 883, 1830
BseMI GCAATG 1 cut(s) 441
BseMII CTCAG 3 cut(s) 158, 1179, 1776
BseNI ACTGG 2 cut(s) 180, 562
BseRI GAGGAG 1 cut(s) 874
BseSI GKGCMC 2 cut(s) 1815, 1852
BseXI GCAGC 2 cut(s) 228, 760
BsgI GTGCAG 2 cut(s) 1385, 1768
Bsh1236I CGCG 2 cut(s) 1707, 1773
Bsh1285I CGRYCG 1 cut(s) 522
BshFI GGCC 1 cut(s) 945
BshVI ATCGAT 1 cut(s) 656
BsiEI CGRYCG 1 cut(s) 522
BsiHKAI GWGCWC 2 cut(s) 1815, 1852
BsiSI CCGG 1 cut(s) 1319
BslFI GGGAC 1 cut(s) 522
BslI CCNNNNNNNGG 2 cut(s) 883, 1830
BsmAI GTCTC 3 cut(s) 181, 581, 856
BsmBI CGTCTC 2 cut(s) 181, 581
BsmFI GGGAC 1 cut(s) 522
BsnI GGCC 1 cut(s) 945
Bsp1286I GDGCHC 2 cut(s) 1815, 1852
Bsp143I GATC 6 cut(s) 262, 604, 1117, 1183, 1328, 1774
Bsp1720I GCTNAGC 1 cut(s) 1323
BspACI CCGC 6 cut(s) 315, 518, 676, 1136, 1268, 1898
BspANI GGCC 1 cut(s) 945
BspCNI CTCAG 3 cut(s) 157, 1178, 1777
BspDI ATCGAT 1 cut(s) 656
BspFNI CGCG 2 cut(s) 1707, 1773
BspLI GGNNCC 3 cut(s) 313, 547, 674
BspPI GGATC 4 cut(s) 257, 612, 1178, 1769
BsrBI CCGCTC 2 cut(s) 1136, 1270
BsrDI GCAATG 1 cut(s) 441
BsrFI RCCGGY 1 cut(s) 1318
BsrI ACTGG 2 cut(s) 180, 562
BssAI RCCGGY 1 cut(s) 1318
BssECI CCNNGG 2 cut(s) 208, 1033
BssMI GATC 6 cut(s) 262, 604, 1117, 1183, 1328, 1774
BssNI GRCGYC 2 cut(s) 57, 723
BssSI CACGAG 1 cut(s) 1401
BssT1I CCWWGG 1 cut(s) 1033
Bst2BI CACGAG 1 cut(s) 1401
Bst2UI CCWGG 1 cut(s) 209
Bst4CI ACNGT 3 cut(s) 235, 1176, 1847
BstACI GRCGYC 2 cut(s) 57, 723
BstAPI GCANNNNNTGC 3 cut(s) 323, 515, 1372
BstBAI YACGTR 1 cut(s) 329
BstC8I GCNNGC 7 cut(s) 526, 947, 1100, 1320, 1421, 1593, 1648
BstDEI CTNAG 5 cut(s) 144, 1165, 1323, 1349, 1785
BstF5I GGATG 5 cut(s) 218, 352, 1023, 1450, 1749
BstFNI CGCG 2 cut(s) 1707, 1773
BstHHI GCGC 1 cut(s) 1773
BstKTI GATC 6 cut(s) 265, 607, 1120, 1186, 1331, 1777
BstMAI GTCTC 3 cut(s) 181, 581, 856
BstMBI GATC 6 cut(s) 262, 604, 1117, 1183, 1328, 1774
BstMCI CGRYCG 1 cut(s) 522
BstMWI GCNNNNNNNGC 5 cut(s) 323, 515, 1324, 1372, 1895
BstNI CCWGG 1 cut(s) 209
BstNSI RCATGY 3 cut(s) 528, 1587, 1717
BstSCI CCNGG 1 cut(s) 207
BstSLI GKGCMC 2 cut(s) 1815, 1852
BstUI CGCG 2 cut(s) 1707, 1773
BstV1I GCAGC 2 cut(s) 228, 760
BstV2I GAAGAC 1 cut(s) 1463
BstX2I RGATCY 1 cut(s) 604
BstYI RGATCY 1 cut(s) 604
Bsu15I ATCGAT 1 cut(s) 656
BsuRI GGCC 1 cut(s) 945
BsuTUI ATCGAT 1 cut(s) 656
BtsCI GGATG 5 cut(s) 218, 352, 1023, 1450, 1749
BtsIMutI CAGTG 1 cut(s) 1852
Cac8I GCNNGC 7 cut(s) 526, 947, 1100, 1320, 1421, 1593, 1648
CaiI CAGNNNCTG 1 cut(s) 151
CfoI GCGC 1 cut(s) 1773
Cfr10I RCCGGY 1 cut(s) 1318
Cfr13I GGNCC 4 cut(s) 160, 311, 1138, 1307
ClaI ATCGAT 1 cut(s) 656
CseI GACGC 1 cut(s) 65
Csp6I GTAC 3 cut(s) 105, 474, 1626
CviQI GTAC 3 cut(s) 105, 474, 1626
DdeI CTNAG 5 cut(s) 144, 1165, 1323, 1349, 1785
DpnI GATC 6 cut(s) 264, 606, 1119, 1185, 1330, 1776
DpnII GATC 6 cut(s) 262, 604, 1117, 1183, 1328, 1774
Eco130I CCWWGG 1 cut(s) 1033
Eco147I AGGCCT 1 cut(s) 945
Eco47I GGWCC 4 cut(s) 160, 311, 1138, 1307
Eco57I CTGAAG 1 cut(s) 1460
Eco72I CACGTG 1 cut(s) 329
EcoRI GAATTC 1 cut(s) 1154
EcoRII CCWGG 1 cut(s) 207
EcoT14I CCWWGG 1 cut(s) 1033
EcoT22I ATGCAT 2 cut(s) 530, 1377
ErhI CCWWGG 1 cut(s) 1033
Esp3I CGTCTC 2 cut(s) 181, 581
FaqI GGGAC 1 cut(s) 522
FauI CCCGC 2 cut(s) 322, 1275
FauNDI CATATG 1 cut(s) 1414
FblI GTMKAC 1 cut(s) 558
Fnu4HI GCNGC 2 cut(s) 217, 774
FokI GGATG 5 cut(s) 225, 359, 1030, 1457, 1756
Fsp4HI GCNGC 2 cut(s) 217, 774
FspBI CTAG 3 cut(s) 68, 581, 1022
GlaI GCGC 1 cut(s) 1772
GluI GCNGC 2 cut(s) 217, 774
GsuI CTGGAG 2 cut(s) 163, 1807
HaeIII GGCC 1 cut(s) 945
HapII CCGG 1 cut(s) 1319
HgaI GACGC 1 cut(s) 65
HhaI GCGC 1 cut(s) 1773
Hin1I GRCGYC 2 cut(s) 57, 723
Hin6I GCGC 1 cut(s) 1771
HinP1I GCGC 1 cut(s) 1771
HincII GTYRAC 6 cut(s) 48, 117, 559, 751, 1843, 1927
HindII GTYRAC 6 cut(s) 48, 117, 559, 751, 1843, 1927
HinfI GANTC 9 cut(s) 38, 644, 737, 831, 887, 995, 1261, 1725, 1820
HpaI GTTAAC 2 cut(s) 117, 1927
HpaII CCGG 1 cut(s) 1319
HphI GGTGA 2 cut(s) 197, 826
Hpy188III TCNNGA 5 cut(s) 496, 741, 1191, 1741, 1824
Hpy99I CGWCG 2 cut(s) 59, 728
HpyAV CCTTC 7 cut(s) 422, 877, 935, 1088, 1378, 1435, 1746
HpyCH4III ACNGT 3 cut(s) 235, 1176, 1847
HpyCH4IV ACGT 6 cut(s) 107, 328, 620, 723, 1488, 1917
HpyF10VI GCNNNNNNNGC 5 cut(s) 323, 515, 1324, 1372, 1895
HpyF3I CTNAG 5 cut(s) 144, 1165, 1323, 1349, 1785
HpySE526I ACGT 6 cut(s) 107, 328, 620, 723, 1488, 1917
Hsp92I GRCGYC 2 cut(s) 57, 723
HspAI GCGC 1 cut(s) 1771
KroI GCCGGC 1 cut(s) 1318
KroNI GCCGGC 1 cut(s) 1320
KspAI GTTAAC 2 cut(s) 117, 1927
Kzo9I GATC 6 cut(s) 262, 604, 1117, 1183, 1328, 1774
Lsp1109I GCAGC 2 cut(s) 228, 760
LweI GCATC 8 cut(s) 203, 237, 627, 648, 883, 904, 1008, 1790
MaeI CTAG 3 cut(s) 68, 581, 1022
MaeII ACGT 6 cut(s) 107, 328, 620, 723, 1488, 1917
MaeIII GTNAC 2 cut(s) 198, 832
MalI GATC 6 cut(s) 264, 606, 1119, 1185, 1330, 1776
MbiI CCGCTC 2 cut(s) 1136, 1270
MboI GATC 6 cut(s) 262, 604, 1117, 1183, 1328, 1774
MboII GAAGA 9 cut(s) 85, 88, 107, 466, 808, 1419, 1463, 1556, 1618
MfeI CAATTG 1 cut(s) 413
MflI RGATCY 1 cut(s) 604
MhlI GDGCHC 2 cut(s) 1815, 1852
MlyI GAGTC 3 cut(s) 840, 896, 1814
MmeI TCCRAC 2 cut(s) 1039, 1698
Mph1103I ATGCAT 2 cut(s) 530, 1377
MroNI GCCGGC 1 cut(s) 1318
MroXI GAANNNNTTC 1 cut(s) 671
MslI CAYNNNNRTG 2 cut(s) 656, 1582
MspI CCGG 1 cut(s) 1319
MspR9I CCNGG 1 cut(s) 209
MunI CAATTG 1 cut(s) 413
MvaI CCWGG 1 cut(s) 209
MvnI CGCG 2 cut(s) 1707, 1773
MwoI GCNNNNNNNGC 5 cut(s) 323, 515, 1324, 1372, 1895
NaeI GCCGGC 1 cut(s) 1320
NdeI CATATG 1 cut(s) 1414
NdeII GATC 6 cut(s) 262, 604, 1117, 1183, 1328, 1774
NgoMIV GCCGGC 1 cut(s) 1318
NlaIV GGNNCC 3 cut(s) 313, 547, 674
NmuCI GTSAC 1 cut(s) 832
NsiI ATGCAT 2 cut(s) 530, 1377
NspI RCATGY 3 cut(s) 528, 1587, 1717
PaeI GCATGC 1 cut(s) 528
PceI AGGCCT 1 cut(s) 945
PciI ACATGT 1 cut(s) 1583
PcsI WCGNNNNNNNCGW 1 cut(s) 662
PdiI GCCGGC 1 cut(s) 1320
PdmI GAANNNNTTC 1 cut(s) 671
PfeI GAWTC 6 cut(s) 38, 644, 737, 995, 1261, 1725
PkrI GCNGC 2 cut(s) 218, 775
PleI GAGTC 3 cut(s) 839, 895, 1814
PmaCI CACGTG 1 cut(s) 329
PmlI CACGTG 1 cut(s) 329
PpsI GAGTC 3 cut(s) 839, 895, 1814
Ppu21I YACGTR 1 cut(s) 329
PscI ACATGT 1 cut(s) 1583
PshBI ATTAAT 2 cut(s) 29, 63
Psp1406I AACGTT 2 cut(s) 620, 1917
Psp6I CCWGG 1 cut(s) 207
PspCI CACGTG 1 cut(s) 329
PspGI CCWGG 1 cut(s) 207
PspN4I GGNNCC 3 cut(s) 313, 547, 674
PspPI GGNCC 4 cut(s) 160, 311, 1138, 1307
PstNI CAGNNNCTG 1 cut(s) 151
PsuI RGATCY 1 cut(s) 604
RsaI GTAC 3 cut(s) 106, 475, 1627
RsaNI GTAC 3 cut(s) 105, 474, 1626
RseI CAYNNNNRTG 2 cut(s) 656, 1582
SalI GTCGAC 1 cut(s) 557
SatI GCNGC 2 cut(s) 217, 774
Sau3AI GATC 6 cut(s) 262, 604, 1117, 1183, 1328, 1774
Sau96I GGNCC 4 cut(s) 160, 311, 1138, 1307
SchI GAGTC 3 cut(s) 840, 896, 1814
ScrFI CCNGG 1 cut(s) 209
SduI GDGCHC 2 cut(s) 1815, 1852
SfaNI GCATC 8 cut(s) 203, 237, 627, 648, 883, 904, 1008, 1790
SinI GGWCC 4 cut(s) 160, 311, 1138, 1307
SmiMI CAYNNNNRTG 2 cut(s) 656, 1582
SphI GCATGC 1 cut(s) 528
SseBI AGGCCT 1 cut(s) 945
SsiI CCGC 6 cut(s) 315, 518, 676, 1136, 1268, 1898
SspI AATATT 5 cut(s) 225, 492, 927, 1213, 1666
SspMI CTAG 3 cut(s) 68, 581, 1022
StuI AGGCCT 1 cut(s) 945
StyD4I CCNGG 1 cut(s) 207
StyI CCWWGG 1 cut(s) 1033
TaaI ACNGT 3 cut(s) 235, 1176, 1847
TaiI ACGT 6 cut(s) 110, 331, 623, 726, 1491, 1920
TaqI TCGA 3 cut(s) 558, 656, 665
TatI WGTACW 1 cut(s) 473
TfiI GAWTC 6 cut(s) 38, 644, 737, 995, 1261, 1725
TscAI CASTG 1 cut(s) 1852
TseFI GTSAC 1 cut(s) 832
TseI GCWGC 2 cut(s) 216, 773
Tsp45I GTSAC 1 cut(s) 832
TspGWI ACGGA 3 cut(s) 558, 1396, 1865
TspRI CASTG 1 cut(s) 1852
VneI GTGCAC 2 cut(s) 1811, 1848
VpaK11BI GGWCC 4 cut(s) 160, 311, 1138, 1307
VspI ATTAAT 2 cut(s) 29, 63
XapI RAATTY 2 cut(s) 1154, 1736
XceI RCATGY 3 cut(s) 528, 1587, 1717
XcmI CCANNNNNNNNNTGG 1 cut(s) 774
XmiI GTMKAC 1 cut(s) 558
XmnI GAANNNNTTC 1 cut(s) 671
XspI CTAG 3 cut(s) 68, 581, 1022
ZraI GACGTC 1 cut(s) 724
Zsp2I ATGCAT 2 cut(s) 530, 1377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.