Rw6G041520

protein desumoylation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0002409
Physical Location & Seq
Reverse (-)
65203544 .. 65206529
2986 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G041520.1

Sequence Viewer

Length: 2034 bp
ATGCAGGATAGTAATACATCTCTGATATTAATATCAGATTCTATGTCAACGGGCGACGCCATTAATTTAGTGAAGAAGAACGAAATACAAGATGAAGAAGTGTGTACGTTTATGTTAACGCCAACGCAATTCATTGAAACCACTCAGGGTCTGACAATGGACCAAGCAAATGTCGTCTCCAGTATCGGCTTCGGGGAGTTACATCACCTGGGTTGCAGCACAATATTTGACACGGTATGTGAGATGATGCTTAATAGCATTGATCCTCTAAATGAGATTGTGTTGATACATGGCAAGGAAATACCCTTTGGACCCACAGATTTTGCACGTGTCATGGGCATAAAGGATGGTGGATTAGACATTCAGAACATGGGAGATACGAACGAAAGTCTTGTTAGGTTAATTAGGAAACAATTGGGGGGTGGAGAAGGTGGACTACACATTGCAAGTTTGAAGAAATGTATCATGGAAAGTACACAGGCTAATGAAATATTCAAGATAGCTTATGCACTTTATGCGGTTGCATGCATTTTATGTCCCAACGGAACCAATTTGGTCGACCAGTCATTGATTTCTACTCTAGTAGAGACGAACATGATAAAGGATCTGAATTGGGCAACGTTCTGCTACTATAAGATGCAGGAATCAGTCATATCAATGCAATCGAAAGGGTTCCGCAACCAAGCAATTTGTGTGCTATTCCTACAAAACTTTTACTTTGATGTCGTTGATAATGAATCCAAATGGGTTGACAAAGGAATCACTCCAATCGCTGCATGGAACACTACTTTAGTGAAGAAATTGATTAGCTGGGTGGAACAAAATGGGGGAGTTACCAACACAAACCAGAATGTTTGGAGGACACAACCTATAATCCATGAGAAGGAGACAACCTATATGATGCAAAACAACCTATATGATGCAAAACAAATATTGTTGAGTGTAGAAGGCCTGCATAAAAGGGTTTCTAATATGGAACAAAAAGAAAGTCGACTGACAGAATCAATGGAAAGAATGGAGAGGATGCTAGGCTCATTCCAAGGGGCAACAATTAAACATATCGTTGGAGAGGTGTTGGCTCGTTTAGAACAGCGAGATGAAGGCAAGCATGGGGAGTGCCAAGATCAATTTAAGTTCAGAGCGGACCTTAATGTGCATGAATTCAGAGGCTCAGAGGCACAGTCTAATGATCCATCAAGACTATTGAACGGCAAAAAAATTTGCATGAACCCCCTGCCAGACGAGGTTTGCATGGCTAGAAAGATGAACCCCCCGCTCAAAGTCCAATCAAAATGTTCATTTAGCCCAGATGGTCCTCCGAAGCCGGCTAAGCGATCAATAAAGGAAAAGAAACTAAGGGCTGTCGGGGTGCAGTCAATGCATATACGGAAGGCTCTTTTCAAAACACGAGGAAGAACATATGGCAAGCAAACAAGGGCGGAACTGAAGGATGATGAAAAACAAAAGGAACAAGGTCTTCGTTGCCTATTCAACGTCAACGCAAATACTCCAAAGGAGGACATCGAGTTGTTGACTTTCATTGGGTTTAGTAAGAAGCCAATTAGTAATCTTCAAATGAGATATTACAATGTCACAGTAGCTAAACATGGGGGAGTGATTGTATCAAGAGCAGACATGCAATGCCTACTGCCTGGTCAACTTTTGAGCGACTATATATACATTCCTCTGCGCGATCCTGCATCTGAGGGGCATTGGTATTTGTTGGTGGTGCACAAAGACTCCAGATTTGGGGAAATTGTTGACAGTGCACCTAATGATGATAGGACTTCATTCCGTGAGGAAGTTGCAAGAATGGCGATGAAGATGCTAGACAATGTTTTTGTTGATGATATTAGGGAGGAAATGGGAGTGCTACCTCATTTTACTGATCTTCCATTCATGGATAAGCACAACCACCCGGTTCAAAGAAACTCGTTCGACTGTGGTGTGTTTGTCATAAGAAACATGCAGCATTATGGCACACAATGGAATGAGGATGTGAGTGGCCTCAAACAAGAATCTAATGTTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48

Protein Analysis

677

Amino Acids

76.65

Weight (kDa)

6.22

Isoelectric Point (pI)

38.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C48 PF02902 557 - 666 1.8e-10 Ulp1 protease family, C-terminal catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 1142, 1276
AccI GTMKAC 2 cut(s) 558, 991
AccII CGCG 1 cut(s) 1692
AciI CCGC 5 cut(s) 518, 676, 1142, 1274, 1439
AclI AACGTT 1 cut(s) 620
AclWI GGATC 4 cut(s) 257, 612, 1184, 1688
AcsI RAATTY 2 cut(s) 1160, 1218
AcuI CTGAAG 1 cut(s) 1466
AcvI CACGTG 1 cut(s) 329
AcyI GRCGYC 1 cut(s) 57
AfaI GTAC 2 cut(s) 106, 475
AfiI CCNNNNNNNGG 2 cut(s) 883, 1749
AflIII ACRYGT 1 cut(s) 328
AgsI TTSAA 8 cut(s) 137, 454, 496, 1207, 1402, 1492, 1574, 1925
AjnI CCWGG 2 cut(s) 207, 1651
AjuI GAANNNNNNNTTGG 2 cut(s) 291, 323
AluBI AGCT 3 cut(s) 503, 810, 1601
AluI AGCT 3 cut(s) 503, 810, 1601
Alw21I GWGCWC 2 cut(s) 1734, 1771
Alw26I GTCTC 3 cut(s) 181, 581, 881
Alw44I GTGCAC 2 cut(s) 1730, 1767
AlwI GGATC 4 cut(s) 257, 612, 1184, 1688
AlwNI CAGNNNCTG 1 cut(s) 151
AoxI GGCC 2 cut(s) 949, 2005
ApaLI GTGCAC 2 cut(s) 1730, 1767
ApeKI GCWGC 3 cut(s) 216, 773, 1969
ApoI RAATTY 2 cut(s) 1160, 1218
ArsI GACNNNNNNTTYG 6 cut(s) 973, 1005, 1730, 1762, 1823, 1855
AseI ATTAAT 2 cut(s) 29, 63
Asp700I GAANNNNTTC 1 cut(s) 671
AspLEI GCGC 1 cut(s) 1692
AspS9I GGNCC 4 cut(s) 160, 311, 1144, 1313
AsuC2I CCSGG 1 cut(s) 1919
AsuHPI GGTGA 1 cut(s) 197
AvaII GGWCC 4 cut(s) 160, 311, 1144, 1313
BaeGI GKGCMC 2 cut(s) 1734, 1771
BarI GAAGNNNNNNTAC 2 cut(s) 420, 452
BauI CACGAG 1 cut(s) 1407
BbrPI CACGTG 1 cut(s) 329
BbsI GAAGAC 1 cut(s) 1469
Bbv12I GWGCWC 2 cut(s) 1734, 1771
BbvI GCAGC 3 cut(s) 228, 760, 1981
BccI CCATC 3 cut(s) 341, 1201, 1304
BceAI ACGGC 1 cut(s) 1225
BcgI CGANNNNNNTGC 2 cut(s) 1807, 1841
BciT130I CCWGG 2 cut(s) 209, 1653
BcnI CCSGG 1 cut(s) 1919
BcoDI GTCTC 3 cut(s) 181, 581, 881
BfaI CTAG 4 cut(s) 581, 1028, 1257, 1829
BisI GCNGC 3 cut(s) 217, 774, 1970
BlpI GCTNAGC 1 cut(s) 1329
BlsI GCNGC 3 cut(s) 218, 775, 1971
Bme1390I CCNGG 3 cut(s) 209, 1653, 1919
Bme18I GGWCC 4 cut(s) 160, 311, 1144, 1313
BmgT120I GGNCC 4 cut(s) 160, 311, 1144, 1313
BmiI GGNNCC 3 cut(s) 313, 547, 674
BmrFI CCNGG 3 cut(s) 209, 1653, 1919
BmsI GCATC 7 cut(s) 237, 627, 891, 910, 1014, 1709, 1815
BpiI GAAGAC 1 cut(s) 1469
BpmI CTGGAG 2 cut(s) 163, 1726
Bpu1102I GCTNAGC 1 cut(s) 1329
BpuMI CCSGG 1 cut(s) 1919
BsaAI YACGTR 1 cut(s) 329
BsaHI GRCGYC 1 cut(s) 57
BsaJI CCNNGG 2 cut(s) 208, 1039
BsaXI ACNNNNNCTCC 4 cut(s) 1605, 1635, 1724, 1754
Bsc4I CCNNNNNNNGG 2 cut(s) 883, 1749
Bse118I RCCGGY 1 cut(s) 1324
Bse1I ACTGG 2 cut(s) 180, 562
Bse3DI GCAATG 2 cut(s) 441, 1646
BseBI CCWGG 2 cut(s) 209, 1653
BseDI CCNNGG 2 cut(s) 208, 1039
BseGI GGATG 4 cut(s) 352, 1029, 1456, 2002
BseLI CCNNNNNNNGG 2 cut(s) 883, 1749
BseMI GCAATG 2 cut(s) 441, 1646
BseMII CTCAG 3 cut(s) 158, 1185, 1695
BseNI ACTGG 2 cut(s) 180, 562
BseSI GKGCMC 2 cut(s) 1734, 1771
BseXI GCAGC 3 cut(s) 228, 760, 1981
BseYI CCCAGC 1 cut(s) 810
BsgI GTGCAG 1 cut(s) 1391
Bsh1236I CGCG 1 cut(s) 1692
BshFI GGCC 2 cut(s) 951, 2007
BsiHKAI GWGCWC 2 cut(s) 1734, 1771
BsiSI CCGG 2 cut(s) 1325, 1919
BslFI GGGAC 1 cut(s) 522
BslI CCNNNNNNNGG 2 cut(s) 883, 1749
BsmAI GTCTC 3 cut(s) 181, 581, 881
BsmBI CGTCTC 2 cut(s) 181, 581
BsmFI GGGAC 1 cut(s) 522
BsnI GGCC 2 cut(s) 951, 2007
Bsp1286I GDGCHC 2 cut(s) 1734, 1771
Bsp143I GATC 7 cut(s) 262, 604, 1123, 1189, 1334, 1693, 1888
Bsp1720I GCTNAGC 1 cut(s) 1329
BspACI CCGC 5 cut(s) 518, 676, 1142, 1274, 1439
BspANI GGCC 2 cut(s) 951, 2007
BspCNI CTCAG 3 cut(s) 157, 1184, 1696
BspFNI CGCG 1 cut(s) 1692
BspLI GGNNCC 3 cut(s) 313, 547, 674
BspPI GGATC 4 cut(s) 257, 612, 1184, 1688
BsrBI CCGCTC 2 cut(s) 1142, 1276
BsrDI GCAATG 2 cut(s) 441, 1646
BsrFI RCCGGY 1 cut(s) 1324
BsrI ACTGG 2 cut(s) 180, 562
BssAI RCCGGY 1 cut(s) 1324
BssECI CCNNGG 2 cut(s) 208, 1039
BssMI GATC 7 cut(s) 262, 604, 1123, 1189, 1334, 1693, 1888
BssNI GRCGYC 1 cut(s) 57
BssSI CACGAG 1 cut(s) 1407
BssT1I CCWWGG 1 cut(s) 1039
Bst2BI CACGAG 1 cut(s) 1407
Bst2UI CCWGG 2 cut(s) 209, 1653
Bst4CI ACNGT 5 cut(s) 235, 1182, 1597, 1766, 1943
BstACI GRCGYC 1 cut(s) 57
BstAPI GCANNNNNTGC 2 cut(s) 515, 1378
BstBAI YACGTR 1 cut(s) 329
BstC8I GCNNGC 5 cut(s) 526, 953, 1106, 1326, 1427
BstDEI CTNAG 5 cut(s) 144, 1171, 1329, 1355, 1704
BstF5I GGATG 4 cut(s) 352, 1029, 1456, 2002
BstFNI CGCG 1 cut(s) 1692
BstHHI GCGC 1 cut(s) 1692
BstKTI GATC 7 cut(s) 265, 607, 1126, 1192, 1337, 1696, 1891
BstMAI GTCTC 3 cut(s) 181, 581, 881
BstMBI GATC 7 cut(s) 262, 604, 1123, 1189, 1334, 1693, 1888
BstMWI GCNNNNNNNGC 4 cut(s) 515, 1330, 1378, 1814
BstNI CCWGG 2 cut(s) 209, 1653
BstNSI RCATGY 3 cut(s) 528, 1639, 1969
BstSCI CCNGG 3 cut(s) 207, 1651, 1917
BstSLI GKGCMC 2 cut(s) 1734, 1771
BstUI CGCG 1 cut(s) 1692
BstV1I GCAGC 3 cut(s) 228, 760, 1981
BstV2I GAAGAC 1 cut(s) 1469
BstX2I RGATCY 1 cut(s) 604
BstYI RGATCY 1 cut(s) 604
BsuRI GGCC 2 cut(s) 951, 2007
BtgZI GCGATG 1 cut(s) 1832
BtsCI GGATG 4 cut(s) 352, 1029, 1456, 2002
BtsIMutI CAGTG 1 cut(s) 1771
Cac8I GCNNGC 5 cut(s) 526, 953, 1106, 1326, 1427
CaiI CAGNNNCTG 1 cut(s) 151
CfoI GCGC 1 cut(s) 1692
Cfr10I RCCGGY 1 cut(s) 1324
Cfr13I GGNCC 4 cut(s) 160, 311, 1144, 1313
CseI GACGC 1 cut(s) 65
Csp6I GTAC 2 cut(s) 105, 474
CspCI CAANNNNNGTGG 2 cut(s) 304, 339
CviQI GTAC 2 cut(s) 105, 474
DdeI CTNAG 5 cut(s) 144, 1171, 1329, 1355, 1704
DpnI GATC 7 cut(s) 264, 606, 1125, 1191, 1336, 1695, 1890
DpnII GATC 7 cut(s) 262, 604, 1123, 1189, 1334, 1693, 1888
EciI GGCGGA 1 cut(s) 1454
Eco130I CCWWGG 1 cut(s) 1039
Eco147I AGGCCT 1 cut(s) 951
Eco47I GGWCC 4 cut(s) 160, 311, 1144, 1313
Eco57I CTGAAG 1 cut(s) 1466
Eco72I CACGTG 1 cut(s) 329
EcoRI GAATTC 1 cut(s) 1160
EcoRII CCWGG 2 cut(s) 207, 1651
EcoT14I CCWWGG 1 cut(s) 1039
EcoT22I ATGCAT 2 cut(s) 530, 1383
ErhI CCWWGG 1 cut(s) 1039
Esp3I CGTCTC 2 cut(s) 181, 581
FaqI GGGAC 1 cut(s) 522
FauI CCCGC 1 cut(s) 1281
FauNDI CATATG 1 cut(s) 1420
FblI GTMKAC 2 cut(s) 558, 991
Fnu4HI GCNGC 3 cut(s) 217, 774, 1970
FokI GGATG 4 cut(s) 359, 1036, 1463, 2009
Fsp4HI GCNGC 3 cut(s) 217, 774, 1970
FspBI CTAG 4 cut(s) 581, 1028, 1257, 1829
GlaI GCGC 1 cut(s) 1691
GluI GCNGC 3 cut(s) 217, 774, 1970
GsaI CCCAGC 1 cut(s) 814
GsuI CTGGAG 2 cut(s) 163, 1726
HaeIII GGCC 2 cut(s) 951, 2007
HapII CCGG 2 cut(s) 1325, 1919
HgaI GACGC 1 cut(s) 65
HhaI GCGC 1 cut(s) 1692
Hin1I GRCGYC 1 cut(s) 57
Hin6I GCGC 1 cut(s) 1690
HinP1I GCGC 1 cut(s) 1690
HincII GTYRAC 9 cut(s) 48, 117, 559, 751, 992, 1498, 1533, 1658, 1762
HindII GTYRAC 9 cut(s) 48, 117, 559, 751, 992, 1498, 1533, 1658, 1762
HinfI GANTC 7 cut(s) 38, 644, 737, 759, 1001, 1739, 2018
HpaI GTTAAC 1 cut(s) 117
HpaII CCGG 2 cut(s) 1325, 1919
HphI GGTGA 1 cut(s) 197
Hpy188III TCNNGA 4 cut(s) 496, 1197, 1626, 1743
Hpy99I CGWCG 1 cut(s) 59
HpyAV CCTTC 6 cut(s) 422, 877, 941, 1094, 1384, 1441
HpyCH4III ACNGT 5 cut(s) 235, 1182, 1597, 1766, 1943
HpyCH4IV ACGT 4 cut(s) 107, 328, 620, 1494
HpyF10VI GCNNNNNNNGC 4 cut(s) 515, 1330, 1378, 1814
HpyF3I CTNAG 5 cut(s) 144, 1171, 1329, 1355, 1704
HpySE526I ACGT 4 cut(s) 107, 328, 620, 1494
Hsp92I GRCGYC 1 cut(s) 57
HspAI GCGC 1 cut(s) 1690
KroI GCCGGC 1 cut(s) 1324
KroNI GCCGGC 1 cut(s) 1326
KspAI GTTAAC 1 cut(s) 117
Kzo9I GATC 7 cut(s) 262, 604, 1123, 1189, 1334, 1693, 1888
Lsp1109I GCAGC 3 cut(s) 228, 760, 1981
LweI GCATC 7 cut(s) 237, 627, 891, 910, 1014, 1709, 1815
MaeI CTAG 4 cut(s) 581, 1028, 1257, 1829
MaeII ACGT 4 cut(s) 107, 328, 620, 1494
MaeIII GTNAC 3 cut(s) 198, 832, 1591
MalI GATC 7 cut(s) 264, 606, 1125, 1191, 1336, 1695, 1890
MbiI CCGCTC 2 cut(s) 1142, 1276
MboI GATC 7 cut(s) 262, 604, 1123, 1189, 1334, 1693, 1888
MfeI CAATTG 1 cut(s) 413
MflI RGATCY 1 cut(s) 604
MhlI GDGCHC 2 cut(s) 1734, 1771
MlyI GAGTC 1 cut(s) 1733
MmeI TCCRAC 1 cut(s) 1045
Mph1103I ATGCAT 2 cut(s) 530, 1383
MroNI GCCGGC 1 cut(s) 1324
MroXI GAANNNNTTC 1 cut(s) 671
MseI TTAA 8 cut(s) 29, 63, 116, 252, 401, 1053, 1131, 1149
MslI CAYNNNNRTG 1 cut(s) 656
MspI CCGG 2 cut(s) 1325, 1919
MspR9I CCNGG 3 cut(s) 209, 1653, 1919
MunI CAATTG 1 cut(s) 413
MvaI CCWGG 2 cut(s) 209, 1653
MvnI CGCG 1 cut(s) 1692
MwoI GCNNNNNNNGC 4 cut(s) 515, 1330, 1378, 1814
NaeI GCCGGC 1 cut(s) 1326
NciI CCSGG 1 cut(s) 1919
NdeI CATATG 1 cut(s) 1420
NdeII GATC 7 cut(s) 262, 604, 1123, 1189, 1334, 1693, 1888
NgoMIV GCCGGC 1 cut(s) 1324
NlaIV GGNNCC 3 cut(s) 313, 547, 674
NmuCI GTSAC 1 cut(s) 1591
NsiI ATGCAT 2 cut(s) 530, 1383
NspI RCATGY 3 cut(s) 528, 1639, 1969
PaeI GCATGC 1 cut(s) 528
PceI AGGCCT 1 cut(s) 951
PdiI GCCGGC 1 cut(s) 1326
PdmI GAANNNNTTC 1 cut(s) 671
PfeI GAWTC 6 cut(s) 38, 644, 737, 759, 1001, 2018
PkrI GCNGC 3 cut(s) 218, 775, 1971
PleI GAGTC 1 cut(s) 1733
PmaCI CACGTG 1 cut(s) 329
PmlI CACGTG 1 cut(s) 329
PpsI GAGTC 1 cut(s) 1733
Ppu21I YACGTR 1 cut(s) 329
PshBI ATTAAT 2 cut(s) 29, 63
Psp1406I AACGTT 1 cut(s) 620
Psp6I CCWGG 2 cut(s) 207, 1651
PspCI CACGTG 1 cut(s) 329
PspFI CCCAGC 1 cut(s) 810
PspGI CCWGG 2 cut(s) 207, 1651
PspN4I GGNNCC 3 cut(s) 313, 547, 674
PspPI GGNCC 4 cut(s) 160, 311, 1144, 1313
PstNI CAGNNNCTG 1 cut(s) 151
PsuI RGATCY 1 cut(s) 604
RsaI GTAC 2 cut(s) 106, 475
RsaNI GTAC 2 cut(s) 105, 474
RseI CAYNNNNRTG 1 cut(s) 656
SalI GTCGAC 2 cut(s) 557, 990
SaqAI TTAA 8 cut(s) 29, 63, 116, 252, 401, 1053, 1131, 1149
SatI GCNGC 3 cut(s) 217, 774, 1970
Sau3AI GATC 7 cut(s) 262, 604, 1123, 1189, 1334, 1693, 1888
Sau96I GGNCC 4 cut(s) 160, 311, 1144, 1313
SchI GAGTC 1 cut(s) 1733
ScrFI CCNGG 3 cut(s) 209, 1653, 1919
SduI GDGCHC 2 cut(s) 1734, 1771
SfaNI GCATC 7 cut(s) 237, 627, 891, 910, 1014, 1709, 1815
SinI GGWCC 4 cut(s) 160, 311, 1144, 1313
SmiMI CAYNNNNRTG 1 cut(s) 656
SphI GCATGC 1 cut(s) 528
SseBI AGGCCT 1 cut(s) 951
SsiI CCGC 5 cut(s) 518, 676, 1142, 1274, 1439
SspI AATATT 3 cut(s) 225, 492, 933
SspMI CTAG 4 cut(s) 581, 1028, 1257, 1829
StuI AGGCCT 1 cut(s) 951
StyD4I CCNGG 3 cut(s) 207, 1651, 1917
StyI CCWWGG 1 cut(s) 1039
TaaI ACNGT 5 cut(s) 235, 1182, 1597, 1766, 1943
TaiI ACGT 4 cut(s) 110, 331, 623, 1497
TaqI TCGA 5 cut(s) 558, 665, 991, 1524, 1938
TatI WGTACW 1 cut(s) 473
TfiI GAWTC 6 cut(s) 38, 644, 737, 759, 1001, 2018
Tru1I TTAA 8 cut(s) 29, 63, 116, 252, 401, 1053, 1131, 1149
Tru9I TTAA 8 cut(s) 29, 63, 116, 252, 401, 1053, 1131, 1149
TscAI CASTG 1 cut(s) 1771
TseFI GTSAC 1 cut(s) 1591
TseI GCWGC 3 cut(s) 216, 773, 1969
Tsp45I GTSAC 1 cut(s) 1591
TspGWI ACGGA 3 cut(s) 558, 1402, 1784
TspRI CASTG 1 cut(s) 1771
VneI GTGCAC 2 cut(s) 1730, 1767
VpaK11BI GGWCC 4 cut(s) 160, 311, 1144, 1313
VspI ATTAAT 2 cut(s) 29, 63
XapI RAATTY 2 cut(s) 1160, 1218
XceI RCATGY 3 cut(s) 528, 1639, 1969
XcmI CCANNNNNNNNNTGG 1 cut(s) 774
XmiI GTMKAC 2 cut(s) 558, 991
XmnI GAANNNNTTC 1 cut(s) 671
XspI CTAG 4 cut(s) 581, 1028, 1257, 1829
Zsp2I ATGCAT 2 cut(s) 530, 1383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.