AT3G02645

UPF0481 protein At3g02645

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0000833
Physical Location & Seq
Forward (+)
566191 .. 567951
1761 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G02645.1

Sequence Viewer

Length: 1590 bp
ATGTTACCTAAAAAACCAATTTTTTCATCTACAGAGCAACACAGGTTTGACGAAACTCGATGGGTCATCAATGTCCAAAAATCTCTCGATGCAGAGCTCGAAGAACACGATCTCGAAGAAGTCACAGTCAGCATTTTCAATGTTCCTAAAGCATTAATGTGTAGCCATCCAGATTCATACACACCTCACCGAGTTTCGATCGGACCTTATCACTGTCTAAAACCAGAGCTTCACGAAATGGAGAGGTATAAACTAATGATCGCGAGAAAAATTCGAAATCAATATAACTCTTTCAGGTTTCATGATCTTGTAGAGAAATTACAATCCATGGAGATTAAGATTCGAGCTTGTTATCATAAGTATATCGGGTTTAACGGAGAGACTCTGTTGTGGATAATGGCGGTAGATTCATCGTTCTTGATCGAGTTTCTTAAGATTTACAGTTTTAGAAAGGTGGAGACTTTGATTAACAGAGTTGGTCATAACGAGATTCTTAGAGATATAATGATGATCGAGAACCAAATCCCACTTTTCGTTTTGAGGAAAACACTCGAGTTTCAGCTTGAATCGACTGAATCAGCTGATGATCTACTGCTCTCTGTTCTTACGGGTCTATGCAAAGATCTCTCTCCTCTCGTTATCAAATTCGACGATGATCAGATTTTGAAGGCTCAGTTTCAAGAGTGTAATCATATCTTAGACTTCTTGTATCAGATGATCGTTCCGAGAATCGAAGAAGAAGAGCTAGAAGAAGATGACGAAGAAAACAGAGCAGACGAAAATGGCGGAAACAGAGCAATTAGGTTTATGGATGAGATTAAGCATCAATTCAAAAGGGTTTTCGCGTCTCGACCTGCGGATTTAATCTTGAGATTCCCGTGGAGAATCATATCGAATCTTCCTGGATTCATGGCTCTGAAACTTTCAGCTGATTATCTCTTCACAAGACAAGAAAACGAAGCAACAACAACGAGACAAGAATCGGTTTCGATTCTTGATATCGAAAAGCCTCCTCTTGTGGAAGAACTTACAATCCCATCAGTCTCCGATCTCCACAAAGCCGGAGTTAGATTCAAACCAACAGCACACGGTAACATTTCAACGGTAACTTTCGATTCAAACTCTGGCCAATTCTACCTCCCTGTAATAAACCTAGACATCAACACAGAAACAGTCCTTCGGAACCTTGTCGCTTACGAAGCTACGAACACTTCAGGACCATTAGTATTCACTCGTTACACAGAGCTAATAAACGGAATAATAGATTCGGAAGAAGACGTTAGGTTGCTTAGAGAACAAGGCGTTCTTGTGAGCCGTCTCAAGAGTGATCAAGAAGCTGCAGAGATGTGGAATGGTATGAGCAAATCTGTGAGGTTGACCAAAGTTGGTTTTTTGGATAAGACTATCGAGGATGTTAACCGTTATTATACCGGACGGTGGAAAGTGAAGATCGGAAGATTGGTTGAGGTTTATGTTTATGGTTCTTGGCAGATTCTAGCGTTTTTGGCGGCGGTTTTGTTGTTGATGTTGGTTTCGTTGCAATTGTTCTCTTTGGTTTTTAGCTCCTTCCTTCGGTTTAGAGCCGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

529

Amino Acids

61.4

Weight (kDa)

5.54

Isoelectric Point (pI)

42.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 45 - 510 4e-105 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 862
AccII CGCG 2 cut(s) 263, 845
AciI CCGC 5 cut(s) 401, 786, 857, 1508, 1511
AcoI YGGCCR 1 cut(s) 1126
AcsI RAATTY 2 cut(s) 270, 644
AcuI CTGAAG 1 cut(s) 1197
AfiI CCNNNNNNNGG 2 cut(s) 1437, 1572
AflII CTTAAG 1 cut(s) 431
AgsI TTSAA 8 cut(s) 139, 566, 667, 680, 832, 1075, 1101, 1119
AjnI CCWGG 1 cut(s) 901
AloI GAACNNNNNNTCC 2 cut(s) 1017, 1049
Alw21I GWGCWC 1 cut(s) 99
Alw26I GTCTC 6 cut(s) 374, 452, 852, 967, 1048, 1322
Ama87I CYCGRG 1 cut(s) 551
AoxI GGCC 1 cut(s) 1126
ApeKI GCWGC 1 cut(s) 1337
ApoI RAATTY 2 cut(s) 270, 644
AseI ATTAAT 1 cut(s) 155
AspS9I GGNCC 2 cut(s) 203, 1217
AsuHPI GGTGA 1 cut(s) 179
AsuII TTCGAA 1 cut(s) 274
AvaI CYCGRG 1 cut(s) 551
AvaII GGWCC 2 cut(s) 203, 1217
BalI TGGCCA 1 cut(s) 1128
BanII GRGCYC 1 cut(s) 99
BbsI GAAGAC 1 cut(s) 1281
Bbv12I GWGCWC 1 cut(s) 99
BbvI GCAGC 1 cut(s) 1324
BccI CCATC 3 cut(s) 54, 174, 1045
BceAI ACGGC 1 cut(s) 1299
BciT130I CCWGG 1 cut(s) 903
BclI TGATCA 2 cut(s) 655, 1327
BcoDI GTCTC 6 cut(s) 374, 452, 852, 967, 1048, 1322
BfaI CTAG 3 cut(s) 746, 1154, 1496
BfmI CTRYAG 2 cut(s) 30, 1338
BfrI CTTAAG 1 cut(s) 431
BfuAI ACCTGC 1 cut(s) 862
BglII AGATCT 1 cut(s) 622
BisI GCNGC 2 cut(s) 1338, 1509
BlsI GCNGC 2 cut(s) 1339, 1510
Bme1390I CCNGG 1 cut(s) 903
Bme18I GGWCC 2 cut(s) 203, 1217
BmeT110I CYCGRG 1 cut(s) 551
BmgT120I GGNCC 2 cut(s) 203, 1217
BmiI GGNNCC 1 cut(s) 1184
BmrFI CCNGG 1 cut(s) 903
BmsI GCATC 2 cut(s) 79, 832
BpiI GAAGAC 1 cut(s) 1281
Bpu14I TTCGAA 1 cut(s) 274
BpuEI CTTGAG 2 cut(s) 889, 1304
BsaJI CCNNGG 2 cut(s) 327, 878
BsaWI WCCGGW 1 cut(s) 1430
Bsc4I CCNNNNNNNGG 2 cut(s) 1437, 1572
Bse118I RCCGGY 1 cut(s) 1583
BseBI CCWGG 1 cut(s) 903
BseDI CCNNGG 2 cut(s) 327, 878
BseGI GGATG 3 cut(s) 166, 817, 1417
BseLI CCNNNNNNNGG 2 cut(s) 1437, 1572
BseMII CTCAG 1 cut(s) 686
BseRI GAGGAG 2 cut(s) 621, 1002
BseXI GCAGC 1 cut(s) 1324
Bsh1236I CGCG 2 cut(s) 263, 845
Bsh1285I CGRYCG 1 cut(s) 201
BshFI GGCC 1 cut(s) 1128
BsiEI CGRYCG 1 cut(s) 201
BsiHKAI GWGCWC 1 cut(s) 99
BsiHKCI CYCGRG 1 cut(s) 551
BsiSI CCGG 3 cut(s) 1062, 1431, 1584
BslI CCNNNNNNNGG 2 cut(s) 1437, 1572
BsmAI GTCTC 6 cut(s) 374, 452, 852, 967, 1048, 1322
BsmBI CGTCTC 2 cut(s) 852, 1322
BsnI GGCC 1 cut(s) 1128
BsoBI CYCGRG 1 cut(s) 551
Bsp119I TTCGAA 1 cut(s) 274
Bsp1286I GDGCHC 1 cut(s) 99
Bsp19I CCATGG 1 cut(s) 327
Bsp68I TCGCGA 1 cut(s) 263
BspACI CCGC 5 cut(s) 401, 786, 857, 1508, 1511
BspANI GGCC 1 cut(s) 1128
BspCNI CTCAG 1 cut(s) 685
BspFNI CGCG 2 cut(s) 263, 845
BspHI TCATGA 1 cut(s) 301
BspLI GGNNCC 1 cut(s) 1184
BspMAI CTGCAG 1 cut(s) 1342
BspMI ACCTGC 1 cut(s) 862
BspQI GCTCTTC 1 cut(s) 735
BspT104I TTCGAA 1 cut(s) 274
BspTI CTTAAG 1 cut(s) 431
BsrFI RCCGGY 1 cut(s) 1583
BssAI RCCGGY 1 cut(s) 1583
BssECI CCNNGG 2 cut(s) 327, 878
BssT1I CCWWGG 1 cut(s) 327
Bst2UI CCWGG 1 cut(s) 903
Bst4CI ACNGT 8 cut(s) 127, 215, 443, 1091, 1105, 1174, 1421, 1437
Bst6I CTCTTC 2 cut(s) 735, 944
BstAFI CTTAAG 1 cut(s) 431
BstBI TTCGAA 1 cut(s) 274
BstDEI CTNAG 4 cut(s) 494, 672, 697, 1289
BstDSI CCRYGG 2 cut(s) 327, 878
BstF5I GGATG 3 cut(s) 166, 817, 1417
BstFNI CGCG 2 cut(s) 263, 845
BstMAI GTCTC 6 cut(s) 374, 452, 852, 967, 1048, 1322
BstMCI CGRYCG 1 cut(s) 201
BstMWI GCNNNNNNNGC 2 cut(s) 1199, 1505
BstNI CCWGG 1 cut(s) 903
BstSCI CCNGG 1 cut(s) 901
BstSFI CTRYAG 2 cut(s) 30, 1338
BstUI CGCG 2 cut(s) 263, 845
BstV1I GCAGC 1 cut(s) 1324
BstV2I GAAGAC 1 cut(s) 1281
BstX2I RGATCY 1 cut(s) 622
BstYI RGATCY 1 cut(s) 622
BsuRI GGCC 1 cut(s) 1128
BtgI CCRYGG 2 cut(s) 327, 878
BtsCI GGATG 3 cut(s) 166, 817, 1417
BtsIMutI CAGTG 1 cut(s) 211
BtuMI TCGCGA 1 cut(s) 263
BveI ACCTGC 1 cut(s) 862
CciI TCATGA 1 cut(s) 301
Cfr10I RCCGGY 1 cut(s) 1583
Cfr13I GGNCC 2 cut(s) 203, 1217
CseI GACGC 1 cut(s) 834
CviAII CATG 3 cut(s) 302, 328, 910
DdeI CTNAG 4 cut(s) 494, 672, 697, 1289
EaeI YGGCCR 1 cut(s) 1126
Eam1104I CTCTTC 2 cut(s) 735, 944
EarI CTCTTC 2 cut(s) 735, 944
EciI GGCGGA 1 cut(s) 801
Ecl136II GAGCTC 1 cut(s) 97
Eco130I CCWWGG 1 cut(s) 327
Eco24I GRGCYC 1 cut(s) 99
Eco32I GATATC 1 cut(s) 1000
Eco47I GGWCC 2 cut(s) 203, 1217
Eco53kI GAGCTC 1 cut(s) 97
Eco57I CTGAAG 1 cut(s) 1197
Eco88I CYCGRG 1 cut(s) 551
EcoICRI GAGCTC 1 cut(s) 97
EcoRII CCWGG 1 cut(s) 901
EcoRV GATATC 1 cut(s) 1000
EcoT14I CCWWGG 1 cut(s) 327
EcoT38I GRGCYC 1 cut(s) 99
ErhI CCWWGG 1 cut(s) 327
Esp3I CGTCTC 2 cut(s) 852, 1322
FaeI CATG 3 cut(s) 305, 331, 913
FalI AAGNNNNNCTT 2 cut(s) 1290, 1322
FatI CATG 3 cut(s) 301, 327, 909
FbaI TGATCA 2 cut(s) 655, 1327
Fnu4HI GCNGC 2 cut(s) 1338, 1509
FokI GGATG 3 cut(s) 153, 824, 1424
FriOI GRGCYC 1 cut(s) 99
Fsp4HI GCNGC 2 cut(s) 1338, 1509
FspBI CTAG 3 cut(s) 746, 1154, 1496
GluI GCNGC 2 cut(s) 1338, 1509
HaeIII GGCC 1 cut(s) 1128
HapII CCGG 3 cut(s) 1062, 1431, 1584
HgaI GACGC 1 cut(s) 834
Hin1II CATG 3 cut(s) 305, 331, 913
HincII GTYRAC 2 cut(s) 1377, 1417
HindII GTYRAC 2 cut(s) 1377, 1417
HpaI GTTAAC 1 cut(s) 1417
HpaII CCGG 3 cut(s) 1062, 1431, 1584
HphI GGTGA 1 cut(s) 179
Hpy166II GTNNAC 2 cut(s) 1377, 1417
Hpy188I TCNGA 9 cut(s) 203, 660, 714, 726, 918, 1048, 1182, 1270, 1454
Hpy8I GTNNAC 2 cut(s) 1377, 1417
Hpy99I CGWCG 1 cut(s) 653
HpyAV CCTTC 4 cut(s) 661, 1187, 1576, 1580
HpyCH4III ACNGT 8 cut(s) 127, 215, 443, 1091, 1105, 1174, 1421, 1437
HpyCH4IV ACGT 1 cut(s) 1278
HpyCH4V TGCA 4 cut(s) 92, 618, 1340, 1540
HpyF10VI GCNNNNNNNGC 2 cut(s) 1199, 1505
HpyF3I CTNAG 4 cut(s) 494, 672, 697, 1289
HpySE526I ACGT 1 cut(s) 1278
Hsp92II CATG 3 cut(s) 305, 331, 913
Ksp22I TGATCA 2 cut(s) 655, 1327
KspAI GTTAAC 1 cut(s) 1417
LguI GCTCTTC 1 cut(s) 735
LmnI GCTCC 1 cut(s) 1568
Lsp1109I GCAGC 1 cut(s) 1324
LweI GCATC 2 cut(s) 79, 832
MaeI CTAG 3 cut(s) 746, 1154, 1496
MaeII ACGT 1 cut(s) 1278
MaeIII GTNAC 5 cut(s) 3, 121, 1091, 1105, 1235
MfeI CAATTG 1 cut(s) 1541
MflI RGATCY 1 cut(s) 622
MhlI GDGCHC 1 cut(s) 99
MlsI TGGCCA 1 cut(s) 1128
MluCI AATT 8 cut(s) 18, 270, 317, 644, 798, 827, 1130, 1541
MluNI TGGCCA 1 cut(s) 1128
MlyI GAGTC 1 cut(s) 376
Mox20I TGGCCA 1 cut(s) 1128
MscI TGGCCA 1 cut(s) 1128
MseI TTAA 8 cut(s) 155, 336, 372, 432, 468, 819, 863, 1416
MslI CAYNNNNRTG 1 cut(s) 157
Msp20I TGGCCA 1 cut(s) 1128
MspA1I CMGCKG 2 cut(s) 581, 929
MspCI CTTAAG 1 cut(s) 431
MspI CCGG 3 cut(s) 1062, 1431, 1584
MspR9I CCNGG 1 cut(s) 903
MunI CAATTG 1 cut(s) 1541
MvaI CCWGG 1 cut(s) 903
MvnI CGCG 2 cut(s) 263, 845
MwoI GCNNNNNNNGC 2 cut(s) 1199, 1505
NcoI CCATGG 1 cut(s) 327
NlaIII CATG 3 cut(s) 305, 331, 913
NlaIV GGNNCC 1 cut(s) 1184
NmuCI GTSAC 1 cut(s) 121
NruI TCGCGA 1 cut(s) 263
NspV TTCGAA 1 cut(s) 274
PaeR7I CTCGAG 1 cut(s) 551
PagI TCATGA 1 cut(s) 301
PciSI GCTCTTC 1 cut(s) 735
PcsI WCGNNNNNNNCGW 1 cut(s) 105
PfoI TCCNGGA 1 cut(s) 901
PkrI GCNGC 2 cut(s) 1339, 1510
Ple19I CGATCG 1 cut(s) 201
PleI GAGTC 1 cut(s) 376
PpsI GAGTC 1 cut(s) 376
PshBI ATTAAT 1 cut(s) 155
Psp124BI GAGCTC 1 cut(s) 99
Psp6I CCWGG 1 cut(s) 901
PspGI CCWGG 1 cut(s) 901
PspN4I GGNNCC 1 cut(s) 1184
PspPI GGNCC 2 cut(s) 203, 1217
PspXI VCTCGAGB 1 cut(s) 551
PstI CTGCAG 1 cut(s) 1342
PsuI RGATCY 1 cut(s) 622
PvuI CGATCG 1 cut(s) 201
PvuII CAGCTG 2 cut(s) 581, 929
RruI TCGCGA 1 cut(s) 263
RseI CAYNNNNRTG 1 cut(s) 157
SacI GAGCTC 1 cut(s) 99
SapI GCTCTTC 1 cut(s) 735
SaqAI TTAA 8 cut(s) 155, 336, 372, 432, 468, 819, 863, 1416
SatI GCNGC 2 cut(s) 1338, 1509
Sau96I GGNCC 2 cut(s) 203, 1217
SchI GAGTC 1 cut(s) 376
ScrFI CCNGG 1 cut(s) 903
SduI GDGCHC 1 cut(s) 99
SfaNI GCATC 2 cut(s) 79, 832
SfcI CTRYAG 2 cut(s) 30, 1338
Sfr274I CTCGAG 1 cut(s) 551
SfuI TTCGAA 1 cut(s) 274
SinI GGWCC 2 cut(s) 203, 1217
SlaI CTCGAG 1 cut(s) 551
SmiMI CAYNNNNRTG 1 cut(s) 157
SmlI CTYRAG 4 cut(s) 431, 551, 868, 1319
SmoI CTYRAG 4 cut(s) 431, 551, 868, 1319
Sse9I AATT 8 cut(s) 18, 270, 317, 644, 798, 827, 1130, 1541
SsiI CCGC 5 cut(s) 401, 786, 857, 1508, 1511
SspMI CTAG 3 cut(s) 746, 1154, 1496
SstI GAGCTC 1 cut(s) 99
StyD4I CCNGG 1 cut(s) 901
StyI CCWWGG 1 cut(s) 327
TaaI ACNGT 8 cut(s) 127, 215, 443, 1091, 1105, 1174, 1421, 1437
TaiI ACGT 1 cut(s) 1281
TasI AATT 8 cut(s) 18, 270, 317, 644, 798, 827, 1130, 1541
TauI GCSGC 1 cut(s) 1511
Tru1I TTAA 8 cut(s) 155, 336, 372, 432, 468, 819, 863, 1416
Tru9I TTAA 8 cut(s) 155, 336, 372, 432, 468, 819, 863, 1416
TscAI CASTG 1 cut(s) 218
TseFI GTSAC 1 cut(s) 121
TseI GCWGC 1 cut(s) 1337
Tsp45I GTSAC 1 cut(s) 121
TspDTI ATGAA 5 cut(s) 15, 165, 290, 399, 898
TspGWI ACGGA 2 cut(s) 390, 1269
TspRI CASTG 1 cut(s) 218
Vha464I CTTAAG 1 cut(s) 431
VpaK11BI GGWCC 2 cut(s) 203, 1217
VspI ATTAAT 1 cut(s) 155
XapI RAATTY 2 cut(s) 270, 644
XhoI CTCGAG 1 cut(s) 551
XspI CTAG 3 cut(s) 746, 1154, 1496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.