Rroxscaffold_7G00216440

UPF0481 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000833
Physical Location & Seq
Reverse (-)
67115531 .. 67117044
1514 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00216440.1

Sequence Viewer

Length: 1407 bp
ATGGCTAGTGACCCGGAATCGTACACCCCTCAAGTGGTTGCGATTGGTCCTTACCATCATTGGCGTCCGGAGCTGTATGAGATGGAGAGGTACAAAGTGGCTGCAGCTAAAAGAACCCAAAAGCAGCTCCAAACCCTCAAATTCCAGGACCTTGTTGACCAATTGACAAGGTTCGAGCTGAGGATTCGGGCTTGTTACCACAAGTACTTGGAGTTTAATGGCGAGACTTTGGGGTGGATGATGGCTATTGATTCTTCGTTTTTCCTCGAAATCCTCCAAGTCTATGCTGTCCAAGAAGGGAACACTTCGACGAGAGTTTCTTCAAGGATGGCGCATTTGGTTGACTATGCAGGAAGGAAGTCAGCGCATCATGCCATCCTTAGGGACTTGGTGATGCTTGAGAACCAAATGCCACTATTTGTTTTGAGAGAAGCGTTGGCATTTCAGTTACCGACGTTGGAATGCGCCGACAATATGCTTCTTTCCATGTTTATGGGACTGTGCAAAGAGCTCTCCCCATTCAAGATGAATGATGAAGACATGCCAAAGATCCAAGTGTCGAGCTATTGTCACTTGCTAGACTTTTTATACCGCATGATCACCCCCAAATTGGAAACTAGACCGTCCGAAATTGCAGAAACCGAGGATGGTCCGGCCACACCACACGAGGGAAAGTCTTCAGATAACTCGAATTTCGTCAAACAATTTCTGGAGAAGGTAGGGAAGCTGCTATCGAAGTTGAACAAGGGCCCGATACGTCTTCTGAAAAAAGTTCTGATTTCTCGACCTGTAAAAGTGTTACGCAAACTGCCTTGGACTATCCTTTCCAACCATGCTGGACCCACAATTTTGAAACAACCTCTCGAGTTACTTTCCTCCCAAGACAAAGAAGCAGTCAAACCCGGAGATGATAAATCAAGCAATAGACCGCCGTTGATAGAGGAGATCACCATCCCATCTGTTTCCGTTCTCTCAAAATCAGGTGTCAAGTTCTTGCCCGCAAGGAGCATCAACTGCATCGGGGCCATTGGTTTTACTCGCTACACCGAGTTGATGAACGGAATCATAGACACCGAGGAGGACGCCAAGTCGCTTAGAGAGAAGGGTATAATCTTGAACCGTTTGAAGAGTGATGAAGAAGTGGCAAAGCTGTTCAATGGCATGAGCAGGTCCATTAGATTGACCAAAGTGCCCTTCTTGGATAAGGCCATCAAAGATGCCAACAAGTATTACCATGGTAGATGGAAGGTGAAGATGAGGAAGTCTTTGAAAACTTATGTGTTCGGTTCTTGGCAATTTCTGGCTTCGCTCGCTACCATTTTTCTCCTGCTCTTGATGACATTGGAAGCATTTTGCTCAGTCTATAGCTGCAGTCACATGTTTCATATCAACACCACTAATAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

468

Amino Acids

53.48

Weight (kDa)

9.22

Isoelectric Point (pI)

43.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 3 - 345 1.4e-52 Plant protein of unknown function
DUF247 PF03140 345 - 445 1.5e-27 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1158
AccIII TCCGGA 1 cut(s) 67
AciI CCGC 3 cut(s) 592, 929, 999
AclWI GGATC 1 cut(s) 544
AcoI YGGCCR 1 cut(s) 654
AcsI RAATTY 2 cut(s) 140, 691
AcuI CTGAAG 1 cut(s) 663
AcyI GRCGYC 2 cut(s) 64, 1083
AfaI GTAC 3 cut(s) 23, 92, 206
AfiI CCNNNNNNNGG 4 cut(s) 34, 381, 610, 668
AflIII ACRYGT 1 cut(s) 1377
AgsI TTSAA 8 cut(s) 324, 523, 742, 853, 1117, 1126, 1156, 1270
AhdI GACNNNNNGTC 1 cut(s) 1087
AjnI CCWGG 1 cut(s) 144
AjuI GAANNNNNNNTTGG 2 cut(s) 1178, 1210
AluBI AGCT 9 cut(s) 73, 107, 127, 178, 511, 564, 727, 1150, 1368
AluI AGCT 9 cut(s) 73, 107, 127, 178, 511, 564, 727, 1150, 1368
Alw21I GWGCWC 1 cut(s) 513
Alw26I GTCTC 1 cut(s) 218
AlwI GGATC 1 cut(s) 544
Ama87I CYCGRG 1 cut(s) 863
Aor13HI TCCGGA 1 cut(s) 67
AoxI GGCC 4 cut(s) 654, 748, 1023, 1206
ApaI GGGCCC 1 cut(s) 752
ApeKI GCWGC 5 cut(s) 101, 104, 124, 727, 1368
ApoI RAATTY 2 cut(s) 140, 691
ArsI GACNNNNNNTTYG 2 cut(s) 879, 911
Asp700I GAANNNNTTC 1 cut(s) 676
AspLEI GCGC 3 cut(s) 334, 367, 467
AspS9I GGNCC 8 cut(s) 47, 148, 650, 748, 749, 839, 1023, 1170
AsuC2I CCSGG 2 cut(s) 14, 903
AsuHPI GGTGA 4 cut(s) 403, 592, 940, 1261
AvaI CYCGRG 1 cut(s) 863
AvaII GGWCC 5 cut(s) 47, 148, 650, 839, 1170
AxyI CCTNAGG 1 cut(s) 380
BaeGI GKGCMC 2 cut(s) 752, 1194
BanII GRGCYC 2 cut(s) 513, 752
BauI CACGAG 1 cut(s) 665
BbsI GAAGAC 3 cut(s) 543, 669, 752
Bbv12I GWGCWC 1 cut(s) 513
BbvCI CCTCAGC 1 cut(s) 179
BbvI GCAGC 5 cut(s) 88, 116, 136, 714, 1355
BceAI ACGGC 1 cut(s) 916
BciT130I CCWGG 1 cut(s) 146
BclI TGATCA 1 cut(s) 597
BcnI CCSGG 2 cut(s) 14, 903
BcoDI GTCTC 1 cut(s) 218
BfaI CTAG 3 cut(s) 6, 578, 618
BfmI CTRYAG 3 cut(s) 102, 1363, 1369
BfuAI ACCTGC 1 cut(s) 1158
BisI GCNGC 5 cut(s) 102, 105, 125, 728, 1369
BlsI GCNGC 5 cut(s) 103, 106, 126, 729, 1370
BmcAI AGTACT 1 cut(s) 206
Bme1390I CCNGG 3 cut(s) 14, 146, 903
Bme18I GGWCC 5 cut(s) 47, 148, 650, 839, 1170
BmeRI GACNNNNNGTC 1 cut(s) 1087
BmeT110I CYCGRG 1 cut(s) 863
BmgT120I GGNCC 8 cut(s) 47, 148, 650, 748, 749, 839, 1023, 1170
BmiI GGNNCC 3 cut(s) 750, 841, 1024
BmrFI CCNGG 3 cut(s) 14, 146, 903
BmsI GCATC 5 cut(s) 376, 384, 1017, 1026, 1207
BpiI GAAGAC 3 cut(s) 543, 669, 752
BpmI CTGGAG 1 cut(s) 731
Bpu10I CCTNAGC 1 cut(s) 179
BpuEI CTTGAG 2 cut(s) 15, 419
BpuMI CCSGG 2 cut(s) 14, 903
BsaBI GATNNNNATC 1 cut(s) 950
BsaHI GRCGYC 2 cut(s) 64, 1083
BsaJI CCNNGG 4 cut(s) 642, 812, 1074, 1234
BsaWI WCCGGW 1 cut(s) 67
Bsc4I CCNNNNNNNGG 4 cut(s) 34, 381, 610, 668
Bse21I CCTNAGG 1 cut(s) 380
Bse8I GATNNNNATC 1 cut(s) 950
BseAI TCCGGA 1 cut(s) 67
BseBI CCWGG 1 cut(s) 146
BseDI CCNNGG 4 cut(s) 642, 812, 1074, 1234
BseGI GGATG 5 cut(s) 243, 333, 375, 652, 951
BseJI GATNNNNATC 1 cut(s) 950
BseLI CCNNNNNNNGG 4 cut(s) 34, 381, 610, 668
BseMII CTCAG 2 cut(s) 170, 1371
BseRI GAGGAG 2 cut(s) 956, 1091
BseSI GKGCMC 2 cut(s) 752, 1194
BseXI GCAGC 5 cut(s) 88, 116, 136, 714, 1355
BshFI GGCC 4 cut(s) 656, 750, 1025, 1208
BsiHKAI GWGCWC 1 cut(s) 513
BsiHKCI CYCGRG 1 cut(s) 863
BsiSI CCGG 4 cut(s) 14, 68, 653, 903
BslFI GGGAC 2 cut(s) 398, 510
BslI CCNNNNNNNGG 4 cut(s) 34, 381, 610, 668
BsmAI GTCTC 1 cut(s) 218
BsmFI GGGAC 2 cut(s) 398, 510
BsmI GAATGC 1 cut(s) 467
BsnI GGCC 4 cut(s) 656, 750, 1025, 1208
BsoBI CYCGRG 1 cut(s) 863
Bsp120I GGGCCC 1 cut(s) 748
Bsp1286I GDGCHC 3 cut(s) 513, 752, 1194
Bsp13I TCCGGA 1 cut(s) 67
Bsp143I GATC 3 cut(s) 549, 597, 945
Bsp19I CCATGG 1 cut(s) 1234
BspACI CCGC 3 cut(s) 592, 929, 999
BspANI GGCC 4 cut(s) 656, 750, 1025, 1208
BspCNI CTCAG 2 cut(s) 171, 1370
BspEI TCCGGA 1 cut(s) 67
BspLI GGNNCC 3 cut(s) 750, 841, 1024
BspMAI CTGCAG 2 cut(s) 106, 1373
BspMI ACCTGC 1 cut(s) 1158
BspPI GGATC 1 cut(s) 544
BssECI CCNNGG 4 cut(s) 642, 812, 1074, 1234
BssMI GATC 3 cut(s) 549, 597, 945
BssNI GRCGYC 2 cut(s) 64, 1083
BssSI CACGAG 1 cut(s) 665
BssT1I CCWWGG 2 cut(s) 812, 1234
Bst2BI CACGAG 1 cut(s) 665
Bst2UI CCWGG 1 cut(s) 146
Bst4CI ACNGT 3 cut(s) 501, 624, 1121
Bst6I CTCTTC 1 cut(s) 1121
BstACI GRCGYC 2 cut(s) 64, 1083
BstAPI GCANNNNNTGC 1 cut(s) 1014
BstC8I GCNNGC 2 cut(s) 999, 1311
BstDEI CTNAG 4 cut(s) 179, 380, 1094, 1357
BstDSI CCRYGG 1 cut(s) 1234
BstF5I GGATG 5 cut(s) 243, 333, 375, 652, 951
BstHHI GCGC 3 cut(s) 334, 367, 467
BstKTI GATC 3 cut(s) 552, 600, 948
BstMAI GTCTC 1 cut(s) 218
BstMBI GATC 3 cut(s) 549, 597, 945
BstMWI GCNNNNNNNGC 4 cut(s) 70, 371, 1014, 1310
BstNI CCWGG 1 cut(s) 146
BstNSI RCATGY 2 cut(s) 544, 1381
BstSCI CCNGG 3 cut(s) 12, 144, 901
BstSFI CTRYAG 3 cut(s) 102, 1363, 1369
BstSLI GKGCMC 2 cut(s) 752, 1194
BstV1I GCAGC 5 cut(s) 88, 116, 136, 714, 1355
BstV2I GAAGAC 3 cut(s) 543, 669, 752
BstX2I RGATCY 1 cut(s) 549
BstXI CCANNNNNNTGG 1 cut(s) 493
BstYI RGATCY 1 cut(s) 549
Bsu36I CCTNAGG 1 cut(s) 380
BsuRI GGCC 4 cut(s) 656, 750, 1025, 1208
BtgI CCRYGG 1 cut(s) 1234
BtsCI GGATG 5 cut(s) 243, 333, 375, 652, 951
BveI ACCTGC 1 cut(s) 1158
Cac8I GCNNGC 2 cut(s) 999, 1311
CfoI GCGC 3 cut(s) 334, 367, 467
Cfr13I GGNCC 8 cut(s) 47, 148, 650, 748, 749, 839, 1023, 1170
CseI GACGC 2 cut(s) 53, 1091
Csp6I GTAC 3 cut(s) 22, 91, 205
CviAII CATG 8 cut(s) 371, 487, 541, 595, 833, 1162, 1235, 1378
CviQI GTAC 3 cut(s) 22, 91, 205
DdeI CTNAG 4 cut(s) 179, 380, 1094, 1357
DpnI GATC 3 cut(s) 551, 599, 947
DpnII GATC 3 cut(s) 549, 597, 945
DriI GACNNNNNGTC 1 cut(s) 1087
EaeI YGGCCR 1 cut(s) 654
Eam1104I CTCTTC 1 cut(s) 1121
Eam1105I GACNNNNNGTC 1 cut(s) 1087
EarI CTCTTC 1 cut(s) 1121
Ecl136II GAGCTC 1 cut(s) 511
Eco130I CCWWGG 2 cut(s) 812, 1234
Eco24I GRGCYC 2 cut(s) 513, 752
Eco47I GGWCC 5 cut(s) 47, 148, 650, 839, 1170
Eco53kI GAGCTC 1 cut(s) 511
Eco57I CTGAAG 1 cut(s) 663
Eco81I CCTNAGG 1 cut(s) 380
Eco88I CYCGRG 1 cut(s) 863
EcoICRI GAGCTC 1 cut(s) 511
EcoO109I RGGNCCY 2 cut(s) 148, 748
EcoRII CCWGG 1 cut(s) 144
EcoT14I CCWWGG 2 cut(s) 812, 1234
EcoT38I GRGCYC 2 cut(s) 513, 752
ErhI CCWWGG 2 cut(s) 812, 1234
FaeI CATG 8 cut(s) 374, 490, 544, 598, 836, 1165, 1238, 1381
FaqI GGGAC 2 cut(s) 398, 510
FatI CATG 8 cut(s) 370, 486, 540, 594, 832, 1161, 1234, 1377
FauI CCCGC 1 cut(s) 1006
FbaI TGATCA 1 cut(s) 597
Fnu4HI GCNGC 5 cut(s) 102, 105, 125, 728, 1369
FokI GGATG 5 cut(s) 250, 340, 362, 659, 938
FriOI GRGCYC 2 cut(s) 513, 752
Fsp4HI GCNGC 5 cut(s) 102, 105, 125, 728, 1369
FspBI CTAG 3 cut(s) 6, 578, 618
GlaI GCGC 3 cut(s) 333, 366, 466
GluI GCNGC 5 cut(s) 102, 105, 125, 728, 1369
GsuI CTGGAG 1 cut(s) 731
HaeIII GGCC 4 cut(s) 656, 750, 1025, 1208
HapII CCGG 4 cut(s) 14, 68, 653, 903
HgaI GACGC 2 cut(s) 53, 1091
HhaI GCGC 3 cut(s) 334, 367, 467
Hin1I GRCGYC 2 cut(s) 64, 1083
Hin1II CATG 8 cut(s) 374, 490, 544, 598, 836, 1165, 1238, 1381
Hin6I GCGC 3 cut(s) 332, 365, 465
HinP1I GCGC 3 cut(s) 332, 365, 465
HincII GTYRAC 2 cut(s) 157, 343
HindII GTYRAC 2 cut(s) 157, 343
HinfI GANTC 4 cut(s) 17, 184, 251, 1062
HpaII CCGG 4 cut(s) 14, 68, 653, 903
HphI GGTGA 4 cut(s) 403, 592, 940, 1261
Hpy166II GTNNAC 3 cut(s) 24, 157, 343
Hpy188I TCNGA 4 cut(s) 628, 682, 765, 777
Hpy188III TCNNGA 7 cut(s) 68, 523, 710, 783, 863, 1114, 1333
Hpy8I GTNNAC 3 cut(s) 24, 157, 343
Hpy99I CGWCG 2 cut(s) 313, 457
HpyAV CCTTC 6 cut(s) 290, 348, 709, 1096, 1204, 1240
HpyCH4III ACNGT 3 cut(s) 501, 624, 1121
HpyCH4IV ACGT 2 cut(s) 455, 757
HpyCH4V TGCA 6 cut(s) 104, 350, 504, 635, 1017, 1371
HpyF10VI GCNNNNNNNGC 4 cut(s) 70, 371, 1014, 1310
HpyF3I CTNAG 4 cut(s) 179, 380, 1094, 1357
HpySE526I ACGT 2 cut(s) 455, 757
Hsp92I GRCGYC 2 cut(s) 64, 1083
Hsp92II CATG 8 cut(s) 374, 490, 544, 598, 836, 1165, 1238, 1381
HspAI GCGC 3 cut(s) 332, 365, 465
Kpn2I TCCGGA 1 cut(s) 67
Ksp22I TGATCA 1 cut(s) 597
Kzo9I GATC 3 cut(s) 549, 597, 945
LmnI GCTCC 3 cut(s) 70, 132, 1005
Lsp1109I GCAGC 5 cut(s) 88, 116, 136, 714, 1355
LweI GCATC 5 cut(s) 376, 384, 1017, 1026, 1207
MaeI CTAG 3 cut(s) 6, 578, 618
MaeII ACGT 2 cut(s) 455, 757
MaeIII GTNAC 7 cut(s) 8, 194, 447, 569, 798, 867, 1373
MalI GATC 3 cut(s) 551, 599, 947
MboI GATC 3 cut(s) 549, 597, 945
MboII GAAGA 8 cut(s) 246, 312, 548, 669, 752, 1138, 1148, 1264
MfeI CAATTG 1 cut(s) 161
MflI RGATCY 1 cut(s) 549
MhlI GDGCHC 3 cut(s) 513, 752, 1194
MluCI AATT 8 cut(s) 140, 161, 608, 630, 691, 704, 846, 1295
MmeI TCCRAC 2 cut(s) 438, 852
MroI TCCGGA 1 cut(s) 67
MroXI GAANNNNTTC 1 cut(s) 676
MseI TTAA 1 cut(s) 216
MslI CAYNNNNRTG 1 cut(s) 491
MspI CCGG 4 cut(s) 14, 68, 653, 903
MspR9I CCNGG 3 cut(s) 14, 146, 903
MunI CAATTG 1 cut(s) 161
Mva1269I GAATGC 1 cut(s) 467
MvaI CCWGG 1 cut(s) 146
MwoI GCNNNNNNNGC 4 cut(s) 70, 371, 1014, 1310
NciI CCSGG 2 cut(s) 14, 903
NcoI CCATGG 1 cut(s) 1234
NdeII GATC 3 cut(s) 549, 597, 945
NlaIII CATG 8 cut(s) 374, 490, 544, 598, 836, 1165, 1238, 1381
NlaIV GGNNCC 3 cut(s) 750, 841, 1024
NmuCI GTSAC 3 cut(s) 8, 569, 1373
NspI RCATGY 2 cut(s) 544, 1381
PaeR7I CTCGAG 1 cut(s) 863
PciI ACATGT 1 cut(s) 1377
PctI GAATGC 1 cut(s) 467
PdmI GAANNNNTTC 1 cut(s) 676
PfeI GAWTC 4 cut(s) 17, 184, 251, 1062
PfoI TCCNGGA 1 cut(s) 144
PkrI GCNGC 5 cut(s) 103, 106, 126, 729, 1370
PpuMI RGGWCCY 1 cut(s) 148
PscI ACATGT 1 cut(s) 1377
Psp124BI GAGCTC 1 cut(s) 513
Psp5II RGGWCCY 1 cut(s) 148
Psp6I CCWGG 1 cut(s) 144
PspGI CCWGG 1 cut(s) 144
PspN4I GGNNCC 3 cut(s) 750, 841, 1024
PspOMI GGGCCC 1 cut(s) 748
PspPI GGNCC 8 cut(s) 47, 148, 650, 748, 749, 839, 1023, 1170
PspPPI RGGWCCY 1 cut(s) 148
PstI CTGCAG 2 cut(s) 106, 1373
PsuI RGATCY 1 cut(s) 549
RsaI GTAC 3 cut(s) 23, 92, 206
RsaNI GTAC 3 cut(s) 22, 91, 205
RseI CAYNNNNRTG 1 cut(s) 491
SacI GAGCTC 1 cut(s) 513
SaqAI TTAA 1 cut(s) 216
SatI GCNGC 5 cut(s) 102, 105, 125, 728, 1369
Sau3AI GATC 3 cut(s) 549, 597, 945
Sau96I GGNCC 8 cut(s) 47, 148, 650, 748, 749, 839, 1023, 1170
ScaI AGTACT 1 cut(s) 206
ScrFI CCNGG 3 cut(s) 14, 146, 903
SduI GDGCHC 3 cut(s) 513, 752, 1194
SfaNI GCATC 5 cut(s) 376, 384, 1017, 1026, 1207
SfcI CTRYAG 3 cut(s) 102, 1363, 1369
Sfr274I CTCGAG 1 cut(s) 863
SinI GGWCC 5 cut(s) 47, 148, 650, 839, 1170
SlaI CTCGAG 1 cut(s) 863
SmiMI CAYNNNNRTG 1 cut(s) 491
SmlI CTYRAG 3 cut(s) 30, 398, 863
SmoI CTYRAG 3 cut(s) 30, 398, 863
Sse9I AATT 8 cut(s) 140, 161, 608, 630, 691, 704, 846, 1295
SsiI CCGC 3 cut(s) 592, 929, 999
SspMI CTAG 3 cut(s) 6, 578, 618
SstI GAGCTC 1 cut(s) 513
StyD4I CCNGG 3 cut(s) 12, 144, 901
StyI CCWWGG 2 cut(s) 812, 1234
TaaI ACNGT 3 cut(s) 501, 624, 1121
TaiI ACGT 2 cut(s) 458, 760
TaqI TCGA 8 cut(s) 174, 267, 308, 560, 689, 734, 784, 864
TasI AATT 8 cut(s) 140, 161, 608, 630, 691, 704, 846, 1295
TatI WGTACW 1 cut(s) 204
TfiI GAWTC 4 cut(s) 17, 184, 251, 1062
Tru1I TTAA 1 cut(s) 216
Tru9I TTAA 1 cut(s) 216
TseFI GTSAC 3 cut(s) 8, 569, 1373
TseI GCWGC 5 cut(s) 101, 104, 124, 727, 1368
Tsp45I GTSAC 3 cut(s) 8, 569, 1373
TspDTI ATGAA 5 cut(s) 542, 549, 1070, 1149, 1373
TspGWI ACGGA 2 cut(s) 955, 1074
VpaK11BI GGWCC 5 cut(s) 47, 148, 650, 839, 1170
XapI RAATTY 2 cut(s) 140, 691
XceI RCATGY 2 cut(s) 544, 1381
XhoI CTCGAG 1 cut(s) 863
XmnI GAANNNNTTC 1 cut(s) 676
XspI CTAG 3 cut(s) 6, 578, 618
ZrmI AGTACT 1 cut(s) 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.