pycom17g22260

UPF0481 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0000833
Physical Location & Seq
Reverse (-)
20696574 .. 20698228
1655 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g22260.2

Sequence Viewer

Length: 1392 bp
ATGTCTTCTTTCCAACAAACTTTCTCATCCAAATCCAGTTCGAACTTCAACGAGTTCCAATGGGTTATTCAAATCCGAAAAACACTCGAGGAAGAGCTCGATGATGACAGCGAAATCCCTGTTTCCGTTTTCAATGTCCCGAAAACCCTTTTGGCCAGTAATCCTGATTCCTACACCCCACAAGAAGTTGCAATTGGTCCTTACCATTATTTGCGTCCGGAGCTTTACGAGATGGAGAGGTACAAAGTGGCTGCAGCTAAAAGAACACAAAAGAATCTCCAGTGCCTTAAGTTTCAAAATCTTGTTGACCAATTGATGAACTTTGAGCCGCGGATTCGTGCTTGCTACCACAAGTACCTGGATTTCAATGGTGAGACACTAGGGTGGATGATGGCGATTGATGCATCGTTCTTGCTAGAGATTTTCCAAGTCTATGGAGCGAAAGAAGGGAAGATATTGACAAGAGTTTCCTCAAAGATGTCACGCTTGGTTGACTATTCGGGCAGGAACTCAGCACACCATTCAATTCTAAGGGACTTGGTAATGCTTGAGAACCAAATCCCACTATTTGTGTTGAGAAAAATGTTGGAGTTTCGATTCCAATCGTTGGAAACCGCCGACGAGATGCTGCTTTCGATGTTGATGGGATTGTGCAAAGAGCTTTCACCATGCAAGATGAAGGATGAGGACCTACCAAAGATCAAAGTCTCAGAATGTGCTCACTTGCTAGACTTTTTGTACCAAATGATCACCCCCAAACTAGAACGCCAGCCCTCTGAAATTGTAGAAGCTGAGTTTGTAGAAGCTGAGGATCAAGGTCGAGGCACAGACGAAGACAAGGAGTCAAAATCTAAGAAGAGCCCGAATTACGTTGAACAATTTCTTGAGAAAGTACGGAAGCTACTGTCGAAACTGAACAAAGGCTTCGTACGTCTTCTTAAAAAAATACTTGTATCCGGACCTATTAAAGTGATATTTAAATTGCCTTGGACAATCCTCTCCTGCCTTCCTGGATTTGCACTTTTGAAACAACCTGTTGCTTATTTATTCTCCTCCCAAGACAAAGAAGAAATCAAACCCGAAAATGAGAGTTCCATCAACAGACCACCATTGGTAGAGGAGATTACAATCCCTTCTGTGTCTGATCTTATGAAATCCGGGGTCTGTTTCGTCATCAAATGCATCAGGGCAGTTGGTTTTTACACGCTACACGGAAATGATGAACGGGATTATAGACACGGAGGAGGACGCAAGGCTCAAGTCGATTCTGTTGACAAAAGTGCCATTCTTAGACCAGGTTTATGTGGTTGGTTGCTTGCCAATTCTTGCTTTGCTGGCTGCCTTTTTTCTCTTGCTATTGATGGCATTGCAAGCGTTTTGCTCAGTTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

464

Amino Acids

52.75

Weight (kDa)

6.69

Isoelectric Point (pI)

42.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 607
AccII CGCG 1 cut(s) 331
AccIII TCCGGA 2 cut(s) 217, 956
AciI CCGC 3 cut(s) 329, 331, 615
AclWI GGATC 1 cut(s) 819
AcoI YGGCCR 1 cut(s) 153
AfaI GTAC 5 cut(s) 242, 356, 740, 894, 930
AfiI CCNNNNNNNGG 1 cut(s) 607
AflII CTTAAG 1 cut(s) 287
AgsI TTSAA 8 cut(s) 49, 71, 133, 296, 367, 525, 875, 1027
AhdI GACNNNNNGTC 1 cut(s) 841
AjnI CCWGG 3 cut(s) 357, 1009, 1294
AjuI GAANNNNNNNTTGG 6 cut(s) 51, 83, 134, 166, 177, 209
AleI CACNNNNGTG 1 cut(s) 382
AluBI AGCT 7 cut(s) 97, 223, 257, 661, 791, 806, 901
AluI AGCT 7 cut(s) 97, 223, 257, 661, 791, 806, 901
Alw21I GWGCWC 2 cut(s) 99, 721
Alw26I GTCTC 2 cut(s) 368, 712
AlwI GGATC 1 cut(s) 819
Ama87I CYCGRG 1 cut(s) 86
Aor13HI TCCGGA 2 cut(s) 217, 956
AoxI GGCC 1 cut(s) 153
ApeKI GCWGC 4 cut(s) 251, 254, 628, 1338
Asp700I GAANNNNTTC 1 cut(s) 879
AspS9I GGNCC 3 cut(s) 197, 688, 959
AsuC2I CCSGG 1 cut(s) 1159
AsuHPI GGTGA 3 cut(s) 383, 657, 742
AsuII TTCGAA 1 cut(s) 41
AvaI CYCGRG 1 cut(s) 86
AvaII GGWCC 3 cut(s) 197, 688, 959
BalI TGGCCA 1 cut(s) 155
BanII GRGCYC 2 cut(s) 99, 863
BbsI GAAGAC 2 cut(s) 840, 926
Bbv12I GWGCWC 2 cut(s) 99, 721
BbvCI CCTCAGC 1 cut(s) 807
BbvI GCAGC 4 cut(s) 238, 266, 615, 1325
BccI CCATC 5 cut(s) 226, 385, 637, 1103, 1355
BciT130I CCWGG 3 cut(s) 359, 1011, 1296
BciVI GTATCC 1 cut(s) 964
BclI TGATCA 1 cut(s) 747
BcnI CCSGG 1 cut(s) 1159
BcoDI GTCTC 2 cut(s) 368, 712
BfaI CTAG 4 cut(s) 380, 416, 728, 761
BfmI CTRYAG 1 cut(s) 252
BfrI CTTAAG 1 cut(s) 287
BfuI GTATCC 1 cut(s) 964
BisI GCNGC 5 cut(s) 252, 255, 329, 629, 1339
BlsI GCNGC 5 cut(s) 253, 256, 330, 630, 1340
Bme1390I CCNGG 4 cut(s) 359, 1011, 1159, 1296
Bme18I GGWCC 3 cut(s) 197, 688, 959
BmeRI GACNNNNNGTC 1 cut(s) 841
BmeT110I CYCGRG 1 cut(s) 86
BmgT120I GGNCC 3 cut(s) 197, 688, 959
BmrFI CCNGG 4 cut(s) 359, 1011, 1159, 1296
BmsI GCATC 4 cut(s) 391, 413, 615, 1191
BpiI GAAGAC 2 cut(s) 840, 926
BpmI CTGGAG 1 cut(s) 263
Bpu10I CCTNAGC 1 cut(s) 807
Bpu14I TTCGAA 1 cut(s) 41
BpuEI CTTGAG 3 cut(s) 569, 905, 1242
BpuMI CCSGG 1 cut(s) 1159
BsaBI GATNNNNATC 2 cut(s) 601, 1127
BsaJI CCNNGG 3 cut(s) 329, 986, 1158
BsaWI WCCGGW 2 cut(s) 217, 956
Bsc4I CCNNNNNNNGG 1 cut(s) 607
Bse1I ACTGG 3 cut(s) 36, 156, 280
Bse3DI GCAATG 1 cut(s) 1365
Bse8I GATNNNNATC 2 cut(s) 601, 1127
BseAI TCCGGA 2 cut(s) 217, 956
BseBI CCWGG 3 cut(s) 359, 1011, 1296
BseDI CCNNGG 3 cut(s) 329, 986, 1158
BseGI GGATG 3 cut(s) 26, 393, 688
BseJI GATNNNNATC 2 cut(s) 601, 1127
BseLI CCNNNNNNNGG 1 cut(s) 607
BseMI GCAATG 1 cut(s) 1365
BseMII CTCAG 4 cut(s) 525, 723, 783, 798
BseNI ACTGG 3 cut(s) 36, 156, 280
BseRI GAGGAG 3 cut(s) 1042, 1133, 1257
BseXI GCAGC 4 cut(s) 238, 266, 615, 1325
Bsh1236I CGCG 1 cut(s) 331
BshFI GGCC 1 cut(s) 155
BsiHKAI GWGCWC 2 cut(s) 99, 721
BsiHKCI CYCGRG 1 cut(s) 86
BsiSI CCGG 3 cut(s) 218, 957, 1158
BsiWI CGTACG 1 cut(s) 928
BslFI GGGAC 2 cut(s) 122, 548
BslI CCNNNNNNNGG 1 cut(s) 607
BsmAI GTCTC 2 cut(s) 368, 712
BsmFI GGGAC 2 cut(s) 122, 548
BsnI GGCC 1 cut(s) 155
BsoBI CYCGRG 1 cut(s) 86
Bsp119I TTCGAA 1 cut(s) 41
Bsp1286I GDGCHC 3 cut(s) 99, 721, 863
Bsp13I TCCGGA 2 cut(s) 217, 956
Bsp143I GATC 4 cut(s) 699, 747, 811, 1144
BspACI CCGC 3 cut(s) 329, 331, 615
BspANI GGCC 1 cut(s) 155
BspCNI CTCAG 4 cut(s) 524, 722, 784, 799
BspEI TCCGGA 2 cut(s) 217, 956
BspFNI CGCG 1 cut(s) 331
BspMAI CTGCAG 1 cut(s) 256
BspPI GGATC 1 cut(s) 819
BspQI GCTCTTC 2 cut(s) 87, 851
BspT104I TTCGAA 1 cut(s) 41
BspTI CTTAAG 1 cut(s) 287
BsrDI GCAATG 1 cut(s) 1365
BsrI ACTGG 3 cut(s) 36, 156, 280
BssECI CCNNGG 3 cut(s) 329, 986, 1158
BssMI GATC 4 cut(s) 699, 747, 811, 1144
BssT1I CCWWGG 1 cut(s) 986
Bst2UI CCWGG 3 cut(s) 359, 1011, 1296
Bst4CI ACNGT 1 cut(s) 906
Bst6I CTCTTC 2 cut(s) 87, 851
BstAFI CTTAAG 1 cut(s) 287
BstBI TTCGAA 1 cut(s) 41
BstC8I GCNNGC 5 cut(s) 343, 770, 1317, 1336, 1372
BstDEI CTNAG 8 cut(s) 511, 530, 709, 792, 807, 852, 1289, 1382
BstDSI CCRYGG 1 cut(s) 329
BstF5I GGATG 3 cut(s) 26, 393, 688
BstFNI CGCG 1 cut(s) 331
BstKTI GATC 4 cut(s) 702, 750, 814, 1147
BstMAI GTCTC 2 cut(s) 368, 712
BstMBI GATC 4 cut(s) 699, 747, 811, 1144
BstMWI GCNNNNNNNGC 4 cut(s) 220, 401, 1335, 1371
BstNI CCWGG 3 cut(s) 359, 1011, 1296
BstSCI CCNGG 4 cut(s) 357, 1009, 1157, 1294
BstSFI CTRYAG 1 cut(s) 252
BstUI CGCG 1 cut(s) 331
BstV1I GCAGC 4 cut(s) 238, 266, 615, 1325
BstV2I GAAGAC 2 cut(s) 840, 926
BstXI CCANNNNNNTGG 1 cut(s) 434
BsuI GTATCC 1 cut(s) 964
BsuRI GGCC 1 cut(s) 155
BtgI CCRYGG 1 cut(s) 329
BtsCI GGATG 3 cut(s) 26, 393, 688
BtsIMutI CAGTG 1 cut(s) 287
Cac8I GCNNGC 5 cut(s) 343, 770, 1317, 1336, 1372
Cfr13I GGNCC 3 cut(s) 197, 688, 959
Cfr42I CCGCGG 1 cut(s) 332
CseI GACGC 2 cut(s) 203, 1257
CsiI ACCWGGT 1 cut(s) 1294
Csp6I GTAC 5 cut(s) 241, 355, 739, 893, 929
CviAII CATG 1 cut(s) 669
CviQI GTAC 5 cut(s) 241, 355, 739, 893, 929
DdeI CTNAG 8 cut(s) 511, 530, 709, 792, 807, 852, 1289, 1382
DpnI GATC 4 cut(s) 701, 749, 813, 1146
DpnII GATC 4 cut(s) 699, 747, 811, 1144
DraI TTTAAA 1 cut(s) 979
DriI GACNNNNNGTC 1 cut(s) 841
EaeI YGGCCR 1 cut(s) 153
Eam1104I CTCTTC 2 cut(s) 87, 851
Eam1105I GACNNNNNGTC 1 cut(s) 841
EarI CTCTTC 2 cut(s) 87, 851
Ecl136II GAGCTC 1 cut(s) 97
Eco130I CCWWGG 1 cut(s) 986
Eco24I GRGCYC 2 cut(s) 99, 863
Eco47I GGWCC 3 cut(s) 197, 688, 959
Eco53kI GAGCTC 1 cut(s) 97
Eco88I CYCGRG 1 cut(s) 86
EcoICRI GAGCTC 1 cut(s) 97
EcoO109I RGGNCCY 1 cut(s) 688
EcoRII CCWGG 3 cut(s) 357, 1009, 1294
EcoT14I CCWWGG 1 cut(s) 986
EcoT22I ATGCAT 2 cut(s) 406, 1184
EcoT38I GRGCYC 2 cut(s) 99, 863
ErhI CCWWGG 1 cut(s) 986
FaeI CATG 1 cut(s) 672
FaiI YATR 6 cut(s) 435, 670, 1151, 1233, 1303, 1390
FaqI GGGAC 2 cut(s) 122, 548
FatI CATG 1 cut(s) 668
FbaI TGATCA 1 cut(s) 747
Fnu4HI GCNGC 5 cut(s) 252, 255, 329, 629, 1339
FokI GGATG 3 cut(s) 13, 400, 695
FriOI GRGCYC 2 cut(s) 99, 863
Fsp4HI GCNGC 5 cut(s) 252, 255, 329, 629, 1339
FspBI CTAG 4 cut(s) 380, 416, 728, 761
GluI GCNGC 5 cut(s) 252, 255, 329, 629, 1339
GsuI CTGGAG 1 cut(s) 263
HaeIII GGCC 1 cut(s) 155
HapII CCGG 3 cut(s) 218, 957, 1158
HgaI GACGC 2 cut(s) 203, 1257
Hin1II CATG 1 cut(s) 672
HincII GTYRAC 3 cut(s) 307, 493, 1273
HindII GTYRAC 3 cut(s) 307, 493, 1273
HinfI GANTC 6 cut(s) 167, 274, 334, 597, 842, 1265
HpaII CCGG 3 cut(s) 218, 957, 1158
HphI GGTGA 3 cut(s) 383, 657, 742
Hpy166II GTNNAC 3 cut(s) 307, 493, 1273
Hpy188I TCNGA 4 cut(s) 77, 712, 778, 1144
Hpy188III TCNNGA 5 cut(s) 139, 164, 218, 884, 957
Hpy8I GTNNAC 3 cut(s) 307, 493, 1273
Hpy99I CGWCG 1 cut(s) 623
HpyAV CCTTC 4 cut(s) 440, 673, 1016, 1143
HpyCH4III ACNGT 1 cut(s) 906
HpyCH4IV ACGT 2 cut(s) 870, 931
HpyCH4V TGCA 8 cut(s) 191, 254, 404, 654, 672, 1019, 1182, 1370
HpyF10VI GCNNNNNNNGC 4 cut(s) 220, 401, 1335, 1371
HpyF3I CTNAG 8 cut(s) 511, 530, 709, 792, 807, 852, 1289, 1382
HpySE526I ACGT 2 cut(s) 870, 931
Hsp92II CATG 1 cut(s) 672
Kpn2I TCCGGA 2 cut(s) 217, 956
Ksp22I TGATCA 1 cut(s) 747
KspI CCGCGG 1 cut(s) 332
Kzo9I GATC 4 cut(s) 699, 747, 811, 1144
LguI GCTCTTC 2 cut(s) 87, 851
LmnI GCTCC 2 cut(s) 220, 437
Lsp1109I GCAGC 4 cut(s) 238, 266, 615, 1325
LweI GCATC 4 cut(s) 391, 413, 615, 1191
MabI ACCWGGT 1 cut(s) 1294
MaeI CTAG 4 cut(s) 380, 416, 728, 761
MaeII ACGT 2 cut(s) 870, 931
MaeIII GTNAC 1 cut(s) 480
MalI GATC 4 cut(s) 701, 749, 813, 1146
MboI GATC 4 cut(s) 699, 747, 811, 1144
MboII GAAGA 6 cut(s) 104, 463, 845, 868, 926, 1079
MfeI CAATTG 2 cut(s) 192, 311
MhlI GDGCHC 3 cut(s) 99, 721, 863
MlsI TGGCCA 1 cut(s) 155
MluCI AATT 8 cut(s) 192, 311, 525, 780, 865, 878, 980, 1321
MluNI TGGCCA 1 cut(s) 155
MlyI GAGTC 1 cut(s) 851
MmeI TCCRAC 3 cut(s) 37, 567, 588
Mox20I TGGCCA 1 cut(s) 155
Mph1103I ATGCAT 2 cut(s) 406, 1184
MroI TCCGGA 2 cut(s) 217, 956
MroXI GAANNNNTTC 1 cut(s) 879
MscI TGGCCA 1 cut(s) 155
MseI TTAA 4 cut(s) 288, 939, 966, 978
MslI CAYNNNNRTG 2 cut(s) 382, 1215
Msp20I TGGCCA 1 cut(s) 155
MspA1I CMGCKG 1 cut(s) 331
MspCI CTTAAG 1 cut(s) 287
MspI CCGG 3 cut(s) 218, 957, 1158
MspR9I CCNGG 4 cut(s) 359, 1011, 1159, 1296
MunI CAATTG 2 cut(s) 192, 311
MvaI CCWGG 3 cut(s) 359, 1011, 1296
MvnI CGCG 1 cut(s) 331
MwoI GCNNNNNNNGC 4 cut(s) 220, 401, 1335, 1371
NciI CCSGG 1 cut(s) 1159
NdeII GATC 4 cut(s) 699, 747, 811, 1144
NlaIII CATG 1 cut(s) 672
NmuCI GTSAC 1 cut(s) 480
NsiI ATGCAT 2 cut(s) 406, 1184
NspV TTCGAA 1 cut(s) 41
OliI CACNNNNGTG 1 cut(s) 382
PaeR7I CTCGAG 1 cut(s) 86
PciSI GCTCTTC 2 cut(s) 87, 851
PdmI GAANNNNTTC 1 cut(s) 879
PfeI GAWTC 5 cut(s) 167, 274, 334, 597, 1265
Pfl23II CGTACG 1 cut(s) 928
PflMI CCANNNNNTGG 1 cut(s) 607
PfoI TCCNGGA 1 cut(s) 1009
PkrI GCNGC 5 cut(s) 253, 256, 330, 630, 1340
PleI GAGTC 1 cut(s) 850
PpsI GAGTC 1 cut(s) 850
PpuMI RGGWCCY 1 cut(s) 688
Psp124BI GAGCTC 1 cut(s) 99
Psp5II RGGWCCY 1 cut(s) 688
Psp6I CCWGG 3 cut(s) 357, 1009, 1294
PspGI CCWGG 3 cut(s) 357, 1009, 1294
PspLI CGTACG 1 cut(s) 928
PspPI GGNCC 3 cut(s) 197, 688, 959
PspPPI RGGWCCY 1 cut(s) 688
PspXI VCTCGAGB 1 cut(s) 86
PstI CTGCAG 1 cut(s) 256
RsaI GTAC 5 cut(s) 242, 356, 740, 894, 930
RsaNI GTAC 5 cut(s) 241, 355, 739, 893, 929
RseI CAYNNNNRTG 2 cut(s) 382, 1215
SacI GAGCTC 1 cut(s) 99
SacII CCGCGG 1 cut(s) 332
SapI GCTCTTC 2 cut(s) 87, 851
SaqAI TTAA 4 cut(s) 288, 939, 966, 978
SatI GCNGC 5 cut(s) 252, 255, 329, 629, 1339
Sau3AI GATC 4 cut(s) 699, 747, 811, 1144
Sau96I GGNCC 3 cut(s) 197, 688, 959
SchI GAGTC 1 cut(s) 851
ScrFI CCNGG 4 cut(s) 359, 1011, 1159, 1296
SduI GDGCHC 3 cut(s) 99, 721, 863
SexAI ACCWGGT 1 cut(s) 1294
SfaNI GCATC 4 cut(s) 391, 413, 615, 1191
SfcI CTRYAG 1 cut(s) 252
Sfr274I CTCGAG 1 cut(s) 86
Sfr303I CCGCGG 1 cut(s) 332
SfuI TTCGAA 1 cut(s) 41
SgrBI CCGCGG 1 cut(s) 332
SinI GGWCC 3 cut(s) 197, 688, 959
SlaI CTCGAG 1 cut(s) 86
SmiI ATTTAAAT 1 cut(s) 979
SmiMI CAYNNNNRTG 2 cut(s) 382, 1215
SmlI CTYRAG 5 cut(s) 86, 287, 548, 884, 1257
SmoI CTYRAG 5 cut(s) 86, 287, 548, 884, 1257
Sse9I AATT 8 cut(s) 192, 311, 525, 780, 865, 878, 980, 1321
SsiI CCGC 3 cut(s) 329, 331, 615
SspMI CTAG 4 cut(s) 380, 416, 728, 761
SstI GAGCTC 1 cut(s) 99
StyD4I CCNGG 4 cut(s) 357, 1009, 1157, 1294
StyI CCWWGG 1 cut(s) 986
SwaI ATTTAAAT 1 cut(s) 979
TaaI ACNGT 1 cut(s) 906
TaiI ACGT 2 cut(s) 873, 934
TaqI TCGA 8 cut(s) 41, 87, 99, 595, 635, 820, 908, 1263
TasI AATT 8 cut(s) 192, 311, 525, 780, 865, 878, 980, 1321
TauI GCSGC 1 cut(s) 331
TfiI GAWTC 5 cut(s) 167, 274, 334, 597, 1265
Tru1I TTAA 4 cut(s) 288, 939, 966, 978
Tru9I TTAA 4 cut(s) 288, 939, 966, 978
TscAI CASTG 1 cut(s) 287
TseFI GTSAC 1 cut(s) 480
TseI GCWGC 4 cut(s) 251, 254, 628, 1338
Tsp45I GTSAC 1 cut(s) 480
TspDTI ATGAA 4 cut(s) 332, 692, 1166, 1236
TspGWI ACGGA 4 cut(s) 115, 910, 1227, 1254
TspRI CASTG 1 cut(s) 287
Van91I CCANNNNNTGG 1 cut(s) 607
Vha464I CTTAAG 1 cut(s) 287
VpaK11BI GGWCC 3 cut(s) 197, 688, 959
XhoI CTCGAG 1 cut(s) 86
XmnI GAANNNNTTC 1 cut(s) 879
XspI CTAG 4 cut(s) 380, 416, 728, 761
Zsp2I ATGCAT 2 cut(s) 406, 1184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.