pycom17g22300

UPF0481 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0000833
Physical Location & Seq
Reverse (-)
20729718 .. 20731220
1503 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g22300.2

Sequence Viewer

Length: 1359 bp
ATGGAGAGGTACAAAGTGGCTGCAGCTAAACGAACACAAAAGAATCTCCAGTGCCTTAAGTTCCAAAATCTTGTTGACCAATTGATAAGGTTTGAGCCGTGGATTCGTGCTTGCTACCACAAGTACCTGAATTTCAATGGTGAGACTCTAGGGTGGATGATGGCGATTGATGCATCATTCTTGCTAGAGATGCTCCAATTCTATGGAGCCAAAGAAGGGAAGATTTTGACAAGAGTTTCCTCAAAGATGTCACGCTTGGTTGACTATTCAGGCAGGAACTCAGCACACCATGCAATTCTAAGGGACTTGGTAATGCTCGAGAACCAAATCCCACTATTTGTGTTGAGAAAAATGTTGGAGTTTCAATTCCAATCGTTGGAAACCGCCGACGACATGCTGCTTTCGATGTTGATGGGATTGTGCAAAGAGCTTTCACCCTTCAAGATGAAGGATGAGGACGTGTCGAAGATCAAAGTCTCAGAATGTGCTCACTTGCTAGACTTTTTGTACCAATTAATCACCCCCAAACTAGAACGCCGGCCCTCTGAAATTGTAGAAGCTGAGGATCAAGGCGAAGGCGCAGACGAAGACAAGGATTCAAAATCTACGAAGAGCCCGAATTACGTTAAACAATTTCTTGGGGAAGTATGGAAGCTGCTGTCGAAACTAAACAAAGGGCCGGTACGTCTCCTTAAAAAAATACTTGTATCCGGACCTATTAAAGTAATATTTAGATTGCCTTGGACAATCCTCTCCAGCCTTCCTGGATTTGCACTTTTGAAACAACCTTTTGCTTATTTATTCTCCTCCCAAGACAAAGAAGAAATCAAACCCGAAAATGAGAGTTCCGTAAACATAACGCCATTGGTAGAGGAGATTACAATCCCTTCTGGTTGCATTTCATCTATCTCCTTTGATCCCAAAACGGTCACACTTTACCTCCCCGCAACTAGCTTAGATGTGAACGCAGAGGTGATCCTGAGAAACTTAGTAGCTTATGAAGCATCAAATGCATCAGGGCAGTTGGTTTTTACACGCTACACGGAAATGATGAACGGGATTATAGACACGAAGGAGGACGCAAAGCTGCTTAGAGAAAAAGGTATAATTCTGAACCGTTTGAAGAGTGATGAAGAAGTGGCCAATCTGTTTAATGGTATGAGCAAGTCGATTCGGTTGACGAAAGTGCCATTCTTGGACCAGGCGATTGACGATGTGAACAAGTATTACAACTGTCAATGGAAGGTTAAAATGGCAAAAATTTTCAAGGTTTATGTGCTGCAGTCGCACATTTCGCATCAACACAACCACTACTACTTAGTGGTGATGCTTAATTTAGTTTTTTTTGGGTGTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

453

Amino Acids

51.86

Weight (kDa)

8.59

Isoelectric Point (pI)

42.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 376
AccIII TCCGGA 1 cut(s) 710
AciI CCGC 2 cut(s) 384, 945
AclWI GGATC 3 cut(s) 573, 911, 970
AcoI YGGCCR 1 cut(s) 1140
AcsI RAATTY 2 cut(s) 130, 1260
AfaI GTAC 4 cut(s) 11, 125, 509, 684
AfiI CCNNNNNNNGG 2 cut(s) 216, 376
AflII CTTAAG 1 cut(s) 56
AflIII ACRYGT 1 cut(s) 459
AgsI TTSAA 7 cut(s) 136, 365, 442, 600, 781, 1123, 1267
AjiI CACGTC 1 cut(s) 460
AjnI CCWGG 2 cut(s) 763, 1200
AluBI AGCT 7 cut(s) 26, 430, 560, 655, 954, 995, 1087
AluI AGCT 7 cut(s) 26, 430, 560, 655, 954, 995, 1087
Alw21I GWGCWC 1 cut(s) 490
Alw26I GTCTC 3 cut(s) 137, 481, 692
AlwI GGATC 3 cut(s) 573, 911, 970
Ama87I CYCGRG 1 cut(s) 317
Aor13HI TCCGGA 1 cut(s) 710
AoxI GGCC 3 cut(s) 539, 677, 1140
ApeKI GCWGC 6 cut(s) 20, 23, 397, 655, 1087, 1279
ApoI RAATTY 2 cut(s) 130, 1260
AseI ATTAAT 1 cut(s) 515
AspLEI GCGC 1 cut(s) 581
AspS9I GGNCC 4 cut(s) 540, 677, 713, 1198
AsuHPI GGTGA 5 cut(s) 152, 426, 511, 985, 1336
AvaI CYCGRG 1 cut(s) 317
AvaII GGWCC 2 cut(s) 713, 1198
BalI TGGCCA 1 cut(s) 1142
BanII GRGCYC 1 cut(s) 617
BbsI GAAGAC 1 cut(s) 594
Bbv12I GWGCWC 1 cut(s) 490
BbvCI CCTCAGC 1 cut(s) 561
BbvI GCAGC 6 cut(s) 7, 35, 384, 642, 1074, 1266
BccI CCATC 2 cut(s) 154, 406
BceAI ACGGC 1 cut(s) 82
BciT130I CCWGG 2 cut(s) 765, 1202
BciVI GTATCC 1 cut(s) 718
BcoDI GTCTC 3 cut(s) 137, 481, 692
BfaI CTAG 5 cut(s) 149, 185, 497, 530, 951
BfmI CTRYAG 2 cut(s) 21, 1280
BfrI CTTAAG 1 cut(s) 56
BfuI GTATCC 1 cut(s) 718
BisI GCNGC 6 cut(s) 21, 24, 398, 656, 1088, 1280
BlsI GCNGC 6 cut(s) 22, 25, 399, 657, 1089, 1281
Bme1390I CCNGG 2 cut(s) 765, 1202
Bme18I GGWCC 2 cut(s) 713, 1198
BmeT110I CYCGRG 1 cut(s) 317
BmgBI CACGTC 1 cut(s) 460
BmgT120I GGNCC 4 cut(s) 540, 677, 713, 1198
BmiI GGNNCC 1 cut(s) 208
BmrFI CCNGG 2 cut(s) 765, 1202
BmsI GCATC 7 cut(s) 160, 180, 182, 1013, 1022, 1306, 1317
BpiI GAAGAC 1 cut(s) 594
BpmI CTGGAG 2 cut(s) 32, 739
Bpu10I CCTNAGC 1 cut(s) 561
BsaBI GATNNNNATC 1 cut(s) 881
BsaJI CCNNGG 2 cut(s) 98, 740
BsaWI WCCGGW 1 cut(s) 710
BsaXI ACNNNNNCTCC 2 cut(s) 924, 954
Bsc4I CCNNNNNNNGG 2 cut(s) 216, 376
Bse118I RCCGGY 2 cut(s) 537, 679
Bse1I ACTGG 1 cut(s) 49
Bse8I GATNNNNATC 1 cut(s) 881
BseAI TCCGGA 1 cut(s) 710
BseBI CCWGG 2 cut(s) 765, 1202
BseDI CCNNGG 2 cut(s) 98, 740
BseGI GGATG 2 cut(s) 162, 457
BseJI GATNNNNATC 1 cut(s) 881
BseLI CCNNNNNNNGG 2 cut(s) 216, 376
BseMII CTCAG 4 cut(s) 294, 492, 552, 971
BseNI ACTGG 1 cut(s) 49
BseRI GAGGAG 2 cut(s) 796, 887
BseXI GCAGC 6 cut(s) 7, 35, 384, 642, 1074, 1266
BshFI GGCC 3 cut(s) 541, 679, 1142
BsiHKAI GWGCWC 1 cut(s) 490
BsiHKCI CYCGRG 1 cut(s) 317
BsiSI CCGG 3 cut(s) 538, 680, 711
BslFI GGGAC 1 cut(s) 317
BslI CCNNNNNNNGG 2 cut(s) 216, 376
BsmAI GTCTC 3 cut(s) 137, 481, 692
BsmBI CGTCTC 1 cut(s) 692
BsmFI GGGAC 1 cut(s) 317
BsnI GGCC 3 cut(s) 541, 679, 1142
BsoBI CYCGRG 1 cut(s) 317
Bsp1286I GDGCHC 2 cut(s) 490, 617
Bsp13I TCCGGA 1 cut(s) 710
Bsp143I GATC 4 cut(s) 468, 565, 916, 975
BspACI CCGC 2 cut(s) 384, 945
BspANI GGCC 3 cut(s) 541, 679, 1142
BspCNI CTCAG 4 cut(s) 293, 491, 553, 972
BspEI TCCGGA 1 cut(s) 710
BspLI GGNNCC 1 cut(s) 208
BspMAI CTGCAG 2 cut(s) 25, 1284
BspPI GGATC 3 cut(s) 573, 911, 970
BspQI GCTCTTC 1 cut(s) 605
BspTI CTTAAG 1 cut(s) 56
BsrFI RCCGGY 2 cut(s) 537, 679
BsrI ACTGG 1 cut(s) 49
BssAI RCCGGY 2 cut(s) 537, 679
BssECI CCNNGG 2 cut(s) 98, 740
BssMI GATC 4 cut(s) 468, 565, 916, 975
BssT1I CCWWGG 1 cut(s) 740
Bst2UI CCWGG 2 cut(s) 765, 1202
Bst4CI ACNGT 3 cut(s) 928, 1118, 1235
Bst6I CTCTTC 2 cut(s) 605, 1118
BstAFI CTTAAG 1 cut(s) 56
BstAPI GCANNNNNTGC 2 cut(s) 290, 1010
BstC8I GCNNGC 2 cut(s) 112, 539
BstDEI CTNAG 9 cut(s) 280, 299, 478, 561, 955, 980, 988, 1091, 1318
BstDSI CCRYGG 1 cut(s) 98
BstF5I GGATG 2 cut(s) 162, 457
BstHHI GCGC 1 cut(s) 581
BstKTI GATC 4 cut(s) 471, 568, 919, 978
BstMAI GTCTC 3 cut(s) 137, 481, 692
BstMBI GATC 4 cut(s) 468, 565, 916, 975
BstMWI GCNNNNNNNGC 7 cut(s) 170, 190, 290, 1001, 1010, 1285, 1294
BstNI CCWGG 2 cut(s) 765, 1202
BstNSI RCATGY 1 cut(s) 397
BstSCI CCNGG 2 cut(s) 763, 1200
BstSFI CTRYAG 2 cut(s) 21, 1280
BstV1I GCAGC 6 cut(s) 7, 35, 384, 642, 1074, 1266
BstV2I GAAGAC 1 cut(s) 594
BstXI CCANNNNNNTGG 1 cut(s) 203
BsuI GTATCC 1 cut(s) 718
BsuRI GGCC 3 cut(s) 541, 679, 1142
BtgI CCRYGG 1 cut(s) 98
BtrI CACGTC 1 cut(s) 460
BtsCI GGATG 2 cut(s) 162, 457
BtsIMutI CAGTG 1 cut(s) 56
Cac8I GCNNGC 2 cut(s) 112, 539
CfoI GCGC 1 cut(s) 581
Cfr10I RCCGGY 2 cut(s) 537, 679
Cfr13I GGNCC 4 cut(s) 540, 677, 713, 1198
CseI GACGC 1 cut(s) 1088
Csp6I GTAC 4 cut(s) 10, 124, 508, 683
CviAII CATG 2 cut(s) 290, 394
CviQI GTAC 4 cut(s) 10, 124, 508, 683
DdeI CTNAG 9 cut(s) 280, 299, 478, 561, 955, 980, 988, 1091, 1318
DpnI GATC 4 cut(s) 470, 567, 918, 977
DpnII GATC 4 cut(s) 468, 565, 916, 975
EaeI YGGCCR 1 cut(s) 1140
Eam1104I CTCTTC 2 cut(s) 605, 1118
EarI CTCTTC 2 cut(s) 605, 1118
Eco130I CCWWGG 1 cut(s) 740
Eco24I GRGCYC 1 cut(s) 617
Eco47I GGWCC 2 cut(s) 713, 1198
Eco88I CYCGRG 1 cut(s) 317
EcoRII CCWGG 2 cut(s) 763, 1200
EcoT14I CCWWGG 1 cut(s) 740
EcoT22I ATGCAT 2 cut(s) 175, 1015
EcoT38I GRGCYC 1 cut(s) 617
ErhI CCWWGG 1 cut(s) 740
Esp3I CGTCTC 1 cut(s) 692
FaeI CATG 2 cut(s) 293, 397
FaqI GGGAC 1 cut(s) 317
FatI CATG 2 cut(s) 289, 393
FauI CCCGC 1 cut(s) 952
Fnu4HI GCNGC 6 cut(s) 21, 24, 398, 656, 1088, 1280
FokI GGATG 2 cut(s) 169, 464
FriOI GRGCYC 1 cut(s) 617
Fsp4HI GCNGC 6 cut(s) 21, 24, 398, 656, 1088, 1280
FspBI CTAG 5 cut(s) 149, 185, 497, 530, 951
GlaI GCGC 1 cut(s) 580
GluI GCNGC 6 cut(s) 21, 24, 398, 656, 1088, 1280
GsuI CTGGAG 2 cut(s) 32, 739
HaeIII GGCC 3 cut(s) 541, 679, 1142
HapII CCGG 3 cut(s) 538, 680, 711
HgaI GACGC 1 cut(s) 1088
HhaI GCGC 1 cut(s) 581
Hin1II CATG 2 cut(s) 293, 397
Hin6I GCGC 1 cut(s) 579
HinP1I GCGC 1 cut(s) 579
HincII GTYRAC 3 cut(s) 76, 262, 1179
HindII GTYRAC 3 cut(s) 76, 262, 1179
HinfI GANTC 5 cut(s) 43, 103, 145, 596, 1171
HpaII CCGG 3 cut(s) 538, 680, 711
HphI GGTGA 5 cut(s) 152, 426, 511, 985, 1336
Hpy166II GTNNAC 6 cut(s) 76, 262, 853, 964, 1179, 1219
Hpy188I TCNGA 3 cut(s) 481, 547, 1113
Hpy188III TCNNGA 4 cut(s) 319, 442, 711, 979
Hpy8I GTNNAC 6 cut(s) 76, 262, 853, 964, 1179, 1219
Hpy99I CGWCG 1 cut(s) 392
HpyAV CCTTC 8 cut(s) 209, 442, 448, 569, 770, 897, 1066, 1237
HpyCH4III ACNGT 3 cut(s) 928, 1118, 1235
HpyCH4IV ACGT 3 cut(s) 459, 624, 685
HpyCH4V TGCA 8 cut(s) 23, 173, 293, 423, 773, 897, 1013, 1282
HpyF10VI GCNNNNNNNGC 7 cut(s) 170, 190, 290, 1001, 1010, 1285, 1294
HpyF3I CTNAG 9 cut(s) 280, 299, 478, 561, 955, 980, 988, 1091, 1318
HpySE526I ACGT 3 cut(s) 459, 624, 685
Hsp92II CATG 2 cut(s) 293, 397
HspAI GCGC 1 cut(s) 579
Kpn2I TCCGGA 1 cut(s) 710
KroI GCCGGC 1 cut(s) 537
KroNI GCCGGC 1 cut(s) 539
Kzo9I GATC 4 cut(s) 468, 565, 916, 975
LguI GCTCTTC 1 cut(s) 605
LmnI GCTCC 2 cut(s) 198, 206
Lsp1109I GCAGC 6 cut(s) 7, 35, 384, 642, 1074, 1266
LweI GCATC 7 cut(s) 160, 180, 182, 1013, 1022, 1306, 1317
MaeI CTAG 5 cut(s) 149, 185, 497, 530, 951
MaeII ACGT 3 cut(s) 459, 624, 685
MaeIII GTNAC 2 cut(s) 249, 928
MalI GATC 4 cut(s) 470, 567, 918, 977
MboI GATC 4 cut(s) 468, 565, 916, 975
MboII GAAGA 7 cut(s) 232, 478, 599, 622, 833, 1135, 1145
MfeI CAATTG 1 cut(s) 80
MhlI GDGCHC 2 cut(s) 490, 617
MlsI TGGCCA 1 cut(s) 1142
MluNI TGGCCA 1 cut(s) 1142
MlyI GAGTC 1 cut(s) 139
MmeI TCCRAC 2 cut(s) 336, 357
Mox20I TGGCCA 1 cut(s) 1142
Mph1103I ATGCAT 2 cut(s) 175, 1015
MroI TCCGGA 1 cut(s) 710
MroNI GCCGGC 1 cut(s) 537
MscI TGGCCA 1 cut(s) 1142
MseI TTAA 9 cut(s) 57, 515, 627, 693, 720, 1152, 1248, 1332, 1357
MslI CAYNNNNRTG 1 cut(s) 1046
Msp20I TGGCCA 1 cut(s) 1142
MspCI CTTAAG 1 cut(s) 56
MspI CCGG 3 cut(s) 538, 680, 711
MspR9I CCNGG 2 cut(s) 765, 1202
MunI CAATTG 1 cut(s) 80
MvaI CCWGG 2 cut(s) 765, 1202
MwoI GCNNNNNNNGC 7 cut(s) 170, 190, 290, 1001, 1010, 1285, 1294
NaeI GCCGGC 1 cut(s) 539
NdeII GATC 4 cut(s) 468, 565, 916, 975
NgoMIV GCCGGC 1 cut(s) 537
NlaIII CATG 2 cut(s) 293, 397
NlaIV GGNNCC 1 cut(s) 208
NmuCI GTSAC 2 cut(s) 249, 928
NsiI ATGCAT 2 cut(s) 175, 1015
NspI RCATGY 1 cut(s) 397
PaeR7I CTCGAG 1 cut(s) 317
PciSI GCTCTTC 1 cut(s) 605
PcsI WCGNNNNNNNCGW 1 cut(s) 614
PdiI GCCGGC 1 cut(s) 539
PfeI GAWTC 4 cut(s) 43, 103, 596, 1171
PflMI CCANNNNNTGG 1 cut(s) 376
PfoI TCCNGGA 1 cut(s) 763
PkrI GCNGC 6 cut(s) 22, 25, 399, 657, 1089, 1281
PleI GAGTC 1 cut(s) 139
PpsI GAGTC 1 cut(s) 139
PshBI ATTAAT 1 cut(s) 515
Psp6I CCWGG 2 cut(s) 763, 1200
PspGI CCWGG 2 cut(s) 763, 1200
PspN4I GGNNCC 1 cut(s) 208
PspPI GGNCC 4 cut(s) 540, 677, 713, 1198
PsrI GAACNNNNNNTAC 2 cut(s) 1211, 1243
PstI CTGCAG 2 cut(s) 25, 1284
RsaI GTAC 4 cut(s) 11, 125, 509, 684
RsaNI GTAC 4 cut(s) 10, 124, 508, 683
RseI CAYNNNNRTG 1 cut(s) 1046
SapI GCTCTTC 1 cut(s) 605
SaqAI TTAA 9 cut(s) 57, 515, 627, 693, 720, 1152, 1248, 1332, 1357
SatI GCNGC 6 cut(s) 21, 24, 398, 656, 1088, 1280
Sau3AI GATC 4 cut(s) 468, 565, 916, 975
Sau96I GGNCC 4 cut(s) 540, 677, 713, 1198
SchI GAGTC 1 cut(s) 139
ScrFI CCNGG 2 cut(s) 765, 1202
SduI GDGCHC 2 cut(s) 490, 617
SfaNI GCATC 7 cut(s) 160, 180, 182, 1013, 1022, 1306, 1317
SfcI CTRYAG 2 cut(s) 21, 1280
Sfr274I CTCGAG 1 cut(s) 317
SinI GGWCC 2 cut(s) 713, 1198
SlaI CTCGAG 1 cut(s) 317
SmiMI CAYNNNNRTG 1 cut(s) 1046
SmlI CTYRAG 2 cut(s) 56, 317
SmoI CTYRAG 2 cut(s) 56, 317
SsiI CCGC 2 cut(s) 384, 945
SspI AATATT 1 cut(s) 729
SspMI CTAG 5 cut(s) 149, 185, 497, 530, 951
StyD4I CCNGG 2 cut(s) 763, 1200
StyI CCWWGG 1 cut(s) 740
TaaI ACNGT 3 cut(s) 928, 1118, 1235
TaiI ACGT 3 cut(s) 462, 627, 688
TaqI TCGA 5 cut(s) 318, 404, 464, 662, 1169
TfiI GAWTC 4 cut(s) 43, 103, 596, 1171
Tru1I TTAA 9 cut(s) 57, 515, 627, 693, 720, 1152, 1248, 1332, 1357
Tru9I TTAA 9 cut(s) 57, 515, 627, 693, 720, 1152, 1248, 1332, 1357
TscAI CASTG 1 cut(s) 56
TseFI GTSAC 2 cut(s) 249, 928
TseI GCWGC 6 cut(s) 20, 23, 397, 655, 1087, 1279
Tsp45I GTSAC 2 cut(s) 249, 928
TspDTI ATGAA 5 cut(s) 461, 891, 1014, 1067, 1146
TspGWI ACGGA 2 cut(s) 838, 1058
TspRI CASTG 1 cut(s) 56
Van91I CCANNNNNTGG 1 cut(s) 376
Vha464I CTTAAG 1 cut(s) 56
VpaK11BI GGWCC 2 cut(s) 713, 1198
VspI ATTAAT 1 cut(s) 515
XapI RAATTY 2 cut(s) 130, 1260
XceI RCATGY 1 cut(s) 397
XhoI CTCGAG 1 cut(s) 317
XspI CTAG 5 cut(s) 149, 185, 497, 530, 951
Zsp2I ATGCAT 2 cut(s) 175, 1015
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.