Rh2DG390800

UPF0481 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000833
Physical Location & Seq
Forward (+)
56628063 .. 56630116
2054 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG390800.1

Sequence Viewer

Length: 1590 bp
ATGTCTTCTCTCCAACCAACATTCTCTTCCAAATCCAGTTCCAACTTCAATGAGCTTCAATGGGTGATTCAAATACGAAAAACCCTAGAGGAGGAGCTCGAAGATGATGGAGAAATCCCCGTCTCCATTTTCAACGTCCCCAAATCCCTCTTGGCTACTGACCCGGAATCGTACACCCCTCAACAGGTTGCGATTGGTCCTTACCATTATTGGCGTCCGGAGCTGTATGACATGGAGAGGTACAAAGTGGCTGCGGCAAAACGAACGCGAAAGCAAACCCTCAAATTCCAGCACCTTGTTGACCAATTGATGAGTTTCGAGCCGAGGATTCGTGCTTGTTACCACAAGTACTTGGAGTTTAATGGTGAGACTTTGGGGTGGATGATGGTCATTGATGCTTCGTTTCTCCTCGAAATCCTCCGAGTCTATGCGGTCCAAGACGGGAAGACTTTGATGAGAGTTTTTTCAAGGATGGCGCATTTGGTTGATCATGCAGGAAGGAAGTCAGCACATCATGCAATCCTTAGGGACTTGGTGATGCTTGAGAATCAAATCCCACTGTTTGTATTGAGAAAAGTGTTGGAATTTCAGTTCCCAACGTTGGAATGTGCCGACAATATGCTTCTTTCCATGTTTATGGGATTGTGCAAAGAGCTCTCACCATTCAAGATGAATGATGACGACTTGCAGAAGATCCAAGTCTCGAGTTGTTGTCACTTGCTGGACTTTTTGTACCACATGGTCACCCCCAAGTTAGAAGCTGGGCCGTCGGAAATCACAGAAGCCGAGGGTGGGGAGGCCACACCAGACAAGGGAAAATCATCAGATAACTCGAATTTCGTCAAACAATTTCTTGAAGAGGTGATATCCAAACTGCCTTGGACTATCCTCTCCAACCTTCCTGGATTTGCAATTTTGAAAAAACCTCTCGAGTCCTTAGTTTCCTCTCAAGAAAAAGAAGCAGTCAAACTCGAAGATGAAAATTCGAGCAGTGGCACTAGTAAACCGCCCTTGATAGAGGAGATCACTATTCCATCTGTCTCTGGTCTGTCAAAATCCGGTGTCAAGTTCCTGCCTGCAAATAGCATTTCGAGTATCTCTTTCGATGCTAAAACTTGCTCATTTTACCTCCCCAGTACTAGATTAGATGGGAATACAGAGGTGATCCTGAGAAACTTGGTGGCCTACGAAGCATCAATTGCATCGGGGCCGTTGGTTTTTACTCGCTACACCGAGTTGATGAATGGGATCATAGACACCGAGGAGGATGCCAAGTTGCTTAGAGGGAAAGGTATCATCTTGAACCATTTGAAGAGTGATGAAGAAGTGGCAAAGCTGTTCAATGGCATGAGCAAGTCCATTAGATTGACCAAAGTGCCCTTCTTGGATAAGGCAATCGAAGATGCCAACAAGTATTACAATAGTAGATGGAAGGTGAAGACGATGAATTTTTTTAAAACTTATGTGATTGGTTGCTGGAAAGTTCTGGCTTTGCTCGCTGCCATTTTGTTCCTGCTCTTGATGACAATGCAAACATTTTGCCCAGGAAACAACTGCAGTCGCATATATCAGATCAACACCACAACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

529

Amino Acids

59.92

Weight (kDa)

6.26

Isoelectric Point (pI)

44.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 43 - 507 9.1e-104 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 268
AccIII TCCGGA 1 cut(s) 217
AciI CCGC 3 cut(s) 254, 431, 1007
AclI AACGTT 1 cut(s) 599
AclWI GGATC 3 cut(s) 688, 1159, 1256
AcsI RAATTY 5 cut(s) 284, 584, 835, 982, 1447
AcyI GRCGYC 1 cut(s) 214
AfaI GTAC 5 cut(s) 173, 242, 350, 734, 1138
AfiI CCNNNNNNNGG 5 cut(s) 91, 184, 601, 792, 812
AhlI ACTAGT 1 cut(s) 998
AjnI CCWGG 2 cut(s) 901, 1543
AjuI GAANNNNNNNTTGG 4 cut(s) 10, 42, 1364, 1396
AloI GAACNNNNNNTCC 2 cut(s) 575, 607
AluBI AGCT 6 cut(s) 55, 97, 223, 655, 761, 1336
AluI AGCT 6 cut(s) 55, 97, 223, 655, 761, 1336
Alw21I GWGCWC 2 cut(s) 99, 657
Alw26I GTCTC 4 cut(s) 127, 362, 706, 1045
AlwI GGATC 3 cut(s) 688, 1159, 1256
Ama87I CYCGRG 2 cut(s) 703, 929
Aor13HI TCCGGA 1 cut(s) 217
AoxI GGCC 4 cut(s) 764, 798, 1182, 1208
ApeKI GCWGC 2 cut(s) 251, 1499
ApoI RAATTY 5 cut(s) 284, 584, 835, 982, 1447
AspLEI GCGC 1 cut(s) 478
AspS9I GGNCC 4 cut(s) 197, 433, 764, 1208
AsuC2I CCSGG 1 cut(s) 164
AsuHPI GGTGA 8 cut(s) 76, 377, 547, 651, 736, 874, 1174, 1447
AvaI CYCGRG 2 cut(s) 703, 929
AvaII GGWCC 2 cut(s) 197, 433
AxyI CCTNAGG 1 cut(s) 524
BaeGI GKGCMC 1 cut(s) 1380
BanII GRGCYC 2 cut(s) 99, 657
BbsI GAAGAC 2 cut(s) 452, 1445
Bbv12I GWGCWC 2 cut(s) 99, 657
BbvI GCAGC 2 cut(s) 238, 1486
BccI CCATC 6 cut(s) 101, 379, 466, 1042, 1142, 1422
BceAI ACGGC 2 cut(s) 751, 1195
BcgI CGANNNNNNTGC 2 cut(s) 1250, 1284
BciT130I CCWGG 2 cut(s) 903, 1545
BclI TGATCA 1 cut(s) 487
BcnI CCSGG 1 cut(s) 164
BcoDI GTCTC 4 cut(s) 127, 362, 706, 1045
BcuI ACTAGT 1 cut(s) 998
BfaI CTAG 4 cut(s) 86, 999, 1140, 1588
BfmI CTRYAG 1 cut(s) 1555
BisI GCNGC 3 cut(s) 252, 255, 1500
BlsI GCNGC 3 cut(s) 253, 256, 1501
BmcAI AGTACT 2 cut(s) 350, 1138
Bme1390I CCNGG 3 cut(s) 164, 903, 1545
Bme18I GGWCC 2 cut(s) 197, 433
BmeT110I CYCGRG 2 cut(s) 703, 929
BmgT120I GGNCC 4 cut(s) 197, 433, 764, 1208
BmiI GGNNCC 1 cut(s) 1209
BmrFI CCNGG 3 cut(s) 164, 903, 1545
BmrI ACTGGG 1 cut(s) 1128
BmsI GCATC 7 cut(s) 385, 528, 1096, 1202, 1211, 1258, 1393
BmuI ACTGGG 1 cut(s) 1128
BpiI GAAGAC 2 cut(s) 452, 1445
BpuEI CTTGAG 2 cut(s) 563, 933
BpuMI CCSGG 1 cut(s) 164
BsaHI GRCGYC 1 cut(s) 214
BsaJI CCNNGG 5 cut(s) 323, 786, 878, 1260, 1543
BsaWI WCCGGW 2 cut(s) 217, 1058
Bsc4I CCNNNNNNNGG 5 cut(s) 91, 184, 601, 792, 812
Bse1I ACTGG 2 cut(s) 36, 1134
Bse21I CCTNAGG 1 cut(s) 524
BseAI TCCGGA 1 cut(s) 217
BseBI CCWGG 2 cut(s) 903, 1545
BseDI CCNNGG 5 cut(s) 323, 786, 878, 1260, 1543
BseGI GGATG 3 cut(s) 387, 477, 1273
BseLI CCNNNNNNNGG 5 cut(s) 91, 184, 601, 792, 812
BseMII CTCAG 1 cut(s) 1160
BseNI ACTGG 2 cut(s) 36, 1134
BseRI GAGGAG 5 cut(s) 104, 107, 398, 1034, 1277
BseSI GKGCMC 1 cut(s) 1380
BseXI GCAGC 2 cut(s) 238, 1486
BseYI CCCAGC 1 cut(s) 761
Bsh1236I CGCG 1 cut(s) 268
BshFI GGCC 4 cut(s) 766, 800, 1184, 1210
BsiHKAI GWGCWC 2 cut(s) 99, 657
BsiHKCI CYCGRG 2 cut(s) 703, 929
BsiSI CCGG 3 cut(s) 164, 218, 1059
BslFI GGGAC 2 cut(s) 122, 542
BslI CCNNNNNNNGG 5 cut(s) 91, 184, 601, 792, 812
BsmAI GTCTC 4 cut(s) 127, 362, 706, 1045
BsmBI CGTCTC 1 cut(s) 127
BsmFI GGGAC 2 cut(s) 122, 542
BsnI GGCC 4 cut(s) 766, 800, 1184, 1210
BsoBI CYCGRG 2 cut(s) 703, 929
Bsp1286I GDGCHC 3 cut(s) 99, 657, 1380
Bsp13I TCCGGA 1 cut(s) 217
Bsp143I GATC 6 cut(s) 487, 693, 1023, 1164, 1248, 1572
BspACI CCGC 3 cut(s) 254, 431, 1007
BspANI GGCC 4 cut(s) 766, 800, 1184, 1210
BspCNI CTCAG 1 cut(s) 1161
BspEI TCCGGA 1 cut(s) 217
BspFNI CGCG 1 cut(s) 268
BspLI GGNNCC 1 cut(s) 1209
BspMAI CTGCAG 1 cut(s) 1559
BspPI GGATC 3 cut(s) 688, 1159, 1256
BsrI ACTGG 2 cut(s) 36, 1134
BssECI CCNNGG 5 cut(s) 323, 786, 878, 1260, 1543
BssMI GATC 6 cut(s) 487, 693, 1023, 1164, 1248, 1572
BssNI GRCGYC 1 cut(s) 214
BssT1I CCWWGG 1 cut(s) 878
Bst2UI CCWGG 2 cut(s) 903, 1545
Bst4CI ACNGT 1 cut(s) 561
Bst6I CTCTTC 3 cut(s) 31, 852, 1307
BstACI GRCGYC 1 cut(s) 214
BstAPI GCANNNNNTGC 2 cut(s) 515, 1199
BstC8I GCNNGC 2 cut(s) 1077, 1497
BstDEI CTNAG 4 cut(s) 524, 937, 1169, 1280
BstEII GGTNACC 1 cut(s) 742
BstENI CCTNNNNNAGG 1 cut(s) 89
BstF5I GGATG 3 cut(s) 387, 477, 1273
BstFNI CGCG 1 cut(s) 268
BstHHI GCGC 1 cut(s) 478
BstKTI GATC 6 cut(s) 490, 696, 1026, 1167, 1251, 1575
BstMAI GTCTC 4 cut(s) 127, 362, 706, 1045
BstMBI GATC 6 cut(s) 487, 693, 1023, 1164, 1248, 1572
BstMWI GCNNNNNNNGC 5 cut(s) 220, 515, 1190, 1199, 1496
BstNI CCWGG 2 cut(s) 903, 1545
BstPI GGTNACC 1 cut(s) 742
BstSCI CCNGG 3 cut(s) 162, 901, 1543
BstSFI CTRYAG 1 cut(s) 1555
BstSLI GKGCMC 1 cut(s) 1380
BstUI CGCG 1 cut(s) 268
BstV1I GCAGC 2 cut(s) 238, 1486
BstV2I GAAGAC 2 cut(s) 452, 1445
BstX2I RGATCY 1 cut(s) 693
BstXI CCANNNNNNTGG 1 cut(s) 637
BstYI RGATCY 1 cut(s) 693
Bsu36I CCTNAGG 1 cut(s) 524
BsuRI GGCC 4 cut(s) 766, 800, 1184, 1210
BtsCI GGATG 3 cut(s) 387, 477, 1273
BtsI GCAGTG 1 cut(s) 997
BtsIMutI CAGTG 2 cut(s) 557, 997
Cac8I GCNNGC 2 cut(s) 1077, 1497
CfoI GCGC 1 cut(s) 478
Cfr13I GGNCC 4 cut(s) 197, 433, 764, 1208
CseI GACGC 1 cut(s) 203
Csp6I GTAC 5 cut(s) 172, 241, 349, 733, 1137
CviAII CATG 6 cut(s) 232, 491, 515, 631, 739, 1348
CviQI GTAC 5 cut(s) 172, 241, 349, 733, 1137
DdeI CTNAG 4 cut(s) 524, 937, 1169, 1280
DpnI GATC 6 cut(s) 489, 695, 1025, 1166, 1250, 1574
DpnII GATC 6 cut(s) 487, 693, 1023, 1164, 1248, 1572
DraI TTTAAA 1 cut(s) 1456
Eam1104I CTCTTC 3 cut(s) 31, 852, 1307
EarI CTCTTC 3 cut(s) 31, 852, 1307
Ecl136II GAGCTC 2 cut(s) 97, 655
Eco130I CCWWGG 1 cut(s) 878
Eco24I GRGCYC 2 cut(s) 99, 657
Eco32I GATATC 1 cut(s) 867
Eco47I GGWCC 2 cut(s) 197, 433
Eco53kI GAGCTC 2 cut(s) 97, 655
Eco81I CCTNAGG 1 cut(s) 524
Eco88I CYCGRG 2 cut(s) 703, 929
Eco91I GGTNACC 1 cut(s) 742
EcoICRI GAGCTC 2 cut(s) 97, 655
EcoNI CCTNNNNNAGG 1 cut(s) 89
EcoO65I GGTNACC 1 cut(s) 742
EcoRII CCWGG 2 cut(s) 901, 1543
EcoRV GATATC 1 cut(s) 867
EcoT14I CCWWGG 1 cut(s) 878
EcoT38I GRGCYC 2 cut(s) 99, 657
ErhI CCWWGG 1 cut(s) 878
Esp3I CGTCTC 1 cut(s) 127
FaeI CATG 6 cut(s) 235, 494, 518, 634, 742, 1351
FaqI GGGAC 2 cut(s) 122, 542
FatI CATG 6 cut(s) 231, 490, 514, 630, 738, 1347
FbaI TGATCA 1 cut(s) 487
Fnu4HI GCNGC 3 cut(s) 252, 255, 1500
FokI GGATG 3 cut(s) 394, 484, 1280
FriOI GRGCYC 2 cut(s) 99, 657
Fsp4HI GCNGC 3 cut(s) 252, 255, 1500
FspBI CTAG 4 cut(s) 86, 999, 1140, 1588
GlaI GCGC 1 cut(s) 477
GluI GCNGC 3 cut(s) 252, 255, 1500
GsaI CCCAGC 1 cut(s) 765
HaeIII GGCC 4 cut(s) 766, 800, 1184, 1210
HapII CCGG 3 cut(s) 164, 218, 1059
HgaI GACGC 1 cut(s) 203
HhaI GCGC 1 cut(s) 478
Hin1I GRCGYC 1 cut(s) 214
Hin1II CATG 6 cut(s) 235, 494, 518, 634, 742, 1351
Hin6I GCGC 1 cut(s) 476
HinP1I GCGC 1 cut(s) 476
HincII GTYRAC 1 cut(s) 301
HindII GTYRAC 1 cut(s) 301
HinfI GANTC 6 cut(s) 67, 167, 328, 423, 547, 932
HpaII CCGG 3 cut(s) 164, 218, 1059
HphI GGTGA 8 cut(s) 76, 377, 547, 651, 736, 874, 1174, 1447
Hpy166II GTNNAC 3 cut(s) 174, 301, 1004
Hpy188I TCNGA 4 cut(s) 422, 772, 826, 1572
Hpy188III TCNNGA 9 cut(s) 218, 667, 703, 854, 929, 950, 1168, 1300, 1519
Hpy8I GTNNAC 3 cut(s) 174, 301, 1004
Hpy99I CGWCG 1 cut(s) 772
HpyAV CCTTC 4 cut(s) 492, 908, 1390, 1426
HpyCH4III ACNGT 1 cut(s) 561
HpyCH4IV ACGT 2 cut(s) 135, 599
HpyCH4V TGCA 9 cut(s) 494, 518, 648, 688, 911, 1079, 1202, 1531, 1557
HpyF10VI GCNNNNNNNGC 5 cut(s) 220, 515, 1190, 1199, 1496
HpyF3I CTNAG 4 cut(s) 524, 937, 1169, 1280
HpySE526I ACGT 2 cut(s) 135, 599
Hsp92I GRCGYC 1 cut(s) 214
Hsp92II CATG 6 cut(s) 235, 494, 518, 634, 742, 1351
HspAI GCGC 1 cut(s) 476
Kpn2I TCCGGA 1 cut(s) 217
Ksp22I TGATCA 1 cut(s) 487
Kzo9I GATC 6 cut(s) 487, 693, 1023, 1164, 1248, 1572
LmnI GCTCC 2 cut(s) 94, 220
Lsp1109I GCAGC 2 cut(s) 238, 1486
LweI GCATC 7 cut(s) 385, 528, 1096, 1202, 1211, 1258, 1393
MaeI CTAG 4 cut(s) 86, 999, 1140, 1588
MaeII ACGT 2 cut(s) 135, 599
MaeIII GTNAC 3 cut(s) 338, 713, 742
MalI GATC 6 cut(s) 489, 695, 1025, 1166, 1250, 1574
MboI GATC 6 cut(s) 487, 693, 1023, 1164, 1248, 1572
MfeI CAATTG 2 cut(s) 305, 1197
MflI RGATCY 1 cut(s) 693
MhlI GDGCHC 3 cut(s) 99, 657, 1380
MluCI AATT 9 cut(s) 284, 305, 584, 835, 848, 912, 982, 1197, 1447
MlyI GAGTC 2 cut(s) 432, 941
MmeI TCCRAC 6 cut(s) 37, 66, 561, 582, 750, 918
MroI TCCGGA 1 cut(s) 217
MseI TTAA 2 cut(s) 360, 1455
MslI CAYNNNNRTG 1 cut(s) 635
MspI CCGG 3 cut(s) 164, 218, 1059
MspR9I CCNGG 3 cut(s) 164, 903, 1545
MunI CAATTG 2 cut(s) 305, 1197
MvaI CCWGG 2 cut(s) 903, 1545
MvnI CGCG 1 cut(s) 268
MwoI GCNNNNNNNGC 5 cut(s) 220, 515, 1190, 1199, 1496
NciI CCSGG 1 cut(s) 164
NdeII GATC 6 cut(s) 487, 693, 1023, 1164, 1248, 1572
NlaIII CATG 6 cut(s) 235, 494, 518, 634, 742, 1351
NlaIV GGNNCC 1 cut(s) 1209
NmeAIII GCCGAG 2 cut(s) 348, 811
NmuCI GTSAC 2 cut(s) 713, 742
PaeR7I CTCGAG 2 cut(s) 703, 929
PfeI GAWTC 4 cut(s) 67, 167, 328, 547
PfoI TCCNGGA 1 cut(s) 901
PkrI GCNGC 3 cut(s) 253, 256, 1501
PleI GAGTC 2 cut(s) 431, 940
PpsI GAGTC 2 cut(s) 431, 940
Psp124BI GAGCTC 2 cut(s) 99, 657
Psp1406I AACGTT 1 cut(s) 599
Psp6I CCWGG 2 cut(s) 901, 1543
PspEI GGTNACC 1 cut(s) 742
PspFI CCCAGC 1 cut(s) 761
PspGI CCWGG 2 cut(s) 901, 1543
PspN4I GGNNCC 1 cut(s) 1209
PspPI GGNCC 4 cut(s) 197, 433, 764, 1208
PstI CTGCAG 1 cut(s) 1559
PsuI RGATCY 1 cut(s) 693
RsaI GTAC 5 cut(s) 173, 242, 350, 734, 1138
RsaNI GTAC 5 cut(s) 172, 241, 349, 733, 1137
RseI CAYNNNNRTG 1 cut(s) 635
SacI GAGCTC 2 cut(s) 99, 657
SaqAI TTAA 2 cut(s) 360, 1455
SatI GCNGC 3 cut(s) 252, 255, 1500
Sau3AI GATC 6 cut(s) 487, 693, 1023, 1164, 1248, 1572
Sau96I GGNCC 4 cut(s) 197, 433, 764, 1208
ScaI AGTACT 2 cut(s) 350, 1138
SchI GAGTC 2 cut(s) 432, 941
ScrFI CCNGG 3 cut(s) 164, 903, 1545
SduI GDGCHC 3 cut(s) 99, 657, 1380
SfaNI GCATC 7 cut(s) 385, 528, 1096, 1202, 1211, 1258, 1393
SfcI CTRYAG 1 cut(s) 1555
Sfr274I CTCGAG 2 cut(s) 703, 929
SinI GGWCC 2 cut(s) 197, 433
SlaI CTCGAG 2 cut(s) 703, 929
SmiMI CAYNNNNRTG 1 cut(s) 635
SmlI CTYRAG 4 cut(s) 542, 703, 929, 948
SmoI CTYRAG 4 cut(s) 542, 703, 929, 948
SpeI ACTAGT 1 cut(s) 998
Sse9I AATT 9 cut(s) 284, 305, 584, 835, 848, 912, 982, 1197, 1447
SsiI CCGC 3 cut(s) 254, 431, 1007
SspMI CTAG 4 cut(s) 86, 999, 1140, 1588
SstI GAGCTC 2 cut(s) 99, 657
StyD4I CCNGG 3 cut(s) 162, 901, 1543
StyI CCWWGG 1 cut(s) 878
TaaI ACNGT 1 cut(s) 561
TaiI ACGT 2 cut(s) 138, 602
TasI AATT 9 cut(s) 284, 305, 584, 835, 848, 912, 982, 1197, 1447
TatI WGTACW 2 cut(s) 348, 1136
TauI GCSGC 1 cut(s) 257
TfiI GAWTC 4 cut(s) 67, 167, 328, 547
Tru1I TTAA 2 cut(s) 360, 1455
Tru9I TTAA 2 cut(s) 360, 1455
TscAI CASTG 2 cut(s) 564, 997
TseFI GTSAC 2 cut(s) 713, 742
TseI GCWGC 2 cut(s) 251, 1499
Tsp45I GTSAC 2 cut(s) 713, 742
TspDTI ATGAA 5 cut(s) 686, 993, 1256, 1335, 1460
TspRI CASTG 2 cut(s) 564, 997
VpaK11BI GGWCC 2 cut(s) 197, 433
XagI CCTNNNNNAGG 1 cut(s) 89
XapI RAATTY 5 cut(s) 284, 584, 835, 982, 1447
XcmI CCANNNNNNNNNTGG 1 cut(s) 148
XhoI CTCGAG 2 cut(s) 703, 929
XspI CTAG 4 cut(s) 86, 999, 1140, 1588
ZrmI AGTACT 2 cut(s) 350, 1138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.