Rroxscaffold_2G00110460

UPF0481 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000833
Physical Location & Seq
Reverse (-)
34758146 .. 34761721
3576 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00110460.1

Sequence Viewer

Length: 1368 bp
ATGTCTTCTCTCCAACCAACATTCTCTTCCAAATCCAGTTCCAACTTCAATGAGCTTCAATGGGTGATTCAAATACGAAAAACCCTAGAGGAGGAGCTCGAGGAAGATGGTGAAATCCCTGTCTCCATTTTCAACGTCCCCAAATCCCTCTTAGCTAGTGACCCGGAATCGTACACCCCTCAAGAGGTTGCAATCGGTCCTTACCATTATTGGCGTCCGGAGCTGTATGAGATGGAGAGGTACAAAGTGGCTGCGGCTAAACGAACCCAAAAGCAGCTCCAAACCCTCAAATTCCAGCACCTTGTTGACCAATTGACGAGGTTCGAGCCGAGGATTCGTGCTTGTTACCACAAGTACTTGGAGTTTAATGGTGAGACTTTGGGGTGGATGATGGTCATTGATGCTTCGTTTTTCCTTGAAATCCTCCAAGTCTATGCTGTCCAAGACGGGAAGACTTTGACAAGAGTTTCTTCAAGGATGGCGCATTTGGTTGACCATGCAGGAAAGAAGTCGGCGCATCACGCAATCCTTAGGGACTTGGTGATGCTTGAGAATCAAATCCCACTGTTTGTGTTGAGAAAAGTTTTGGAATTTCAGTTTCCCACGTTGGAATGCGCCGACGAGATGGTTCTTTCCATGTTTATGGGATTGTGCAAAGAGCTCTCACCATTCAAGATGAATGATGAGGACTTGCAGAAGATCCAAGTCTCGAGTTGTTGTCACTTGCTGGACTTTTTGTACCACATGATCACCCCCAAGTTAGAAGCTGGGCCGTCGGAAATCACAGAAGCCGAGGGTGGGGAGGCCACACCACACAAGGGAAAATCATCAGATAACTCGAATTTCGTCAAACAATTTCTGGAGGAGGTATGGAAGCTGCTTTCGAAGTTGAATAAAGGTCCAATACGTCTTCTGAAAAAAATTCTAATATCTGGACCCGTAAAGGTGATATTCAAATTGCCTTGGACTATCCTCTCCAACCTTCCTGGATTTGCTATTTTGAAAAAACCTCTTGAGTCCTTATTTTCCTCCCAAGACAAAGAAGCAGTCAAACCCGAAGATGAAAATTCGAGCAGTGGCATTAGTAAACCACCCTTGATAGAGGAGATCACTATTCCATCTGTCTCTGATTGGTCAAAATCCGGTGTCAAGTTCTTGCCGGCAAATAGTATTTCGAGCATCTCTTTCGATGCTAAGACTTGCTCATTTTACCTCCCGAATACTAGCTTAGATGGGAACACGAGAGGTGATCCCGAGAAACTTGGTGGCCTACGAAGCATCAACTGCATCGGGGCCATTGGTTTTTACTCGCTACACCGAGTTGATGAACGGGATCATAGACACCGAGGAGGACGCCAAGTTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

455

Amino Acids

51.54

Weight (kDa)

6.52

Isoelectric Point (pI)

46.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 43 - 414 5.1e-59 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 217
AciI CCGC 1 cut(s) 254
AclWI GGATC 3 cut(s) 694, 1244, 1341
AcsI RAATTY 5 cut(s) 290, 590, 841, 921, 1066
AcyI GRCGYC 2 cut(s) 214, 1354
AfaI GTAC 4 cut(s) 173, 242, 356, 740
AfiI CCNNNNNNNGG 4 cut(s) 91, 184, 798, 818
AjnI CCWGG 1 cut(s) 985
AjuI GAANNNNNNNTTGG 2 cut(s) 10, 42
AluBI AGCT 9 cut(s) 55, 97, 155, 223, 277, 661, 767, 877, 1227
AluI AGCT 9 cut(s) 55, 97, 155, 223, 277, 661, 767, 877, 1227
Alw21I GWGCWC 2 cut(s) 99, 663
Alw26I GTCTC 4 cut(s) 127, 368, 712, 1129
AlwI GGATC 3 cut(s) 694, 1244, 1341
Ama87I CYCGRG 3 cut(s) 98, 709, 1253
Aor13HI TCCGGA 1 cut(s) 217
AoxI GGCC 4 cut(s) 770, 804, 1267, 1293
ApeKI GCWGC 3 cut(s) 251, 274, 877
ApoI RAATTY 5 cut(s) 290, 590, 841, 921, 1066
ArsI GACNNNNNNTTYG 2 cut(s) 1032, 1064
AspLEI GCGC 3 cut(s) 484, 517, 617
AspS9I GGNCC 5 cut(s) 197, 770, 899, 935, 1293
AsuC2I CCSGG 1 cut(s) 164
AsuHPI GGTGA 8 cut(s) 76, 122, 383, 553, 657, 742, 958, 1259
AsuII TTCGAA 1 cut(s) 884
AvaI CYCGRG 3 cut(s) 98, 709, 1253
AvaII GGWCC 3 cut(s) 197, 899, 935
AxyI CCTNAGG 1 cut(s) 530
BanII GRGCYC 2 cut(s) 99, 663
BauI CACGAG 1 cut(s) 1240
BbsI GAAGAC 2 cut(s) 458, 902
Bbv12I GWGCWC 2 cut(s) 99, 663
BbvI GCAGC 3 cut(s) 238, 286, 864
BccI CCATC 7 cut(s) 101, 226, 385, 472, 619, 1126, 1226
BceAI ACGGC 1 cut(s) 757
BcgI CGANNNNNNTGC 2 cut(s) 1168, 1202
BciT130I CCWGG 1 cut(s) 987
BclI TGATCA 1 cut(s) 747
BcnI CCSGG 1 cut(s) 164
BcoDI GTCTC 4 cut(s) 127, 368, 712, 1129
BfaI CTAG 3 cut(s) 86, 156, 1224
BisI GCNGC 4 cut(s) 252, 255, 275, 878
BlsI GCNGC 4 cut(s) 253, 256, 276, 879
BmcAI AGTACT 1 cut(s) 356
Bme1390I CCNGG 2 cut(s) 164, 987
Bme18I GGWCC 3 cut(s) 197, 899, 935
BmeT110I CYCGRG 3 cut(s) 98, 709, 1253
BmgT120I GGNCC 5 cut(s) 197, 770, 899, 935, 1293
BmiI GGNNCC 2 cut(s) 937, 1294
BmrFI CCNGG 2 cut(s) 164, 987
BmsI GCATC 7 cut(s) 391, 526, 534, 1180, 1188, 1287, 1296
BpiI GAAGAC 2 cut(s) 458, 902
BpmI CTGGAG 1 cut(s) 881
Bpu14I TTCGAA 1 cut(s) 884
BpuEI CTTGAG 3 cut(s) 165, 569, 1034
BpuMI CCSGG 1 cut(s) 164
BsaHI GRCGYC 2 cut(s) 214, 1354
BsaJI CCNNGG 4 cut(s) 329, 792, 962, 1345
BsaWI WCCGGW 2 cut(s) 217, 1142
Bsc4I CCNNNNNNNGG 4 cut(s) 91, 184, 798, 818
Bse118I RCCGGY 1 cut(s) 1159
Bse1I ACTGG 1 cut(s) 36
Bse21I CCTNAGG 1 cut(s) 530
BseAI TCCGGA 1 cut(s) 217
BseBI CCWGG 1 cut(s) 987
BseDI CCNNGG 4 cut(s) 329, 792, 962, 1345
BseGI GGATG 2 cut(s) 393, 483
BseLI CCNNNNNNNGG 4 cut(s) 91, 184, 798, 818
BseNI ACTGG 1 cut(s) 36
BseRI GAGGAG 5 cut(s) 104, 107, 878, 1118, 1362
BseXI GCAGC 3 cut(s) 238, 286, 864
BseYI CCCAGC 1 cut(s) 767
BshFI GGCC 4 cut(s) 772, 806, 1269, 1295
BsiHKAI GWGCWC 2 cut(s) 99, 663
BsiHKCI CYCGRG 3 cut(s) 98, 709, 1253
BsiSI CCGG 4 cut(s) 164, 218, 1143, 1160
BslFI GGGAC 2 cut(s) 122, 548
BslI CCNNNNNNNGG 4 cut(s) 91, 184, 798, 818
BsmAI GTCTC 4 cut(s) 127, 368, 712, 1129
BsmFI GGGAC 2 cut(s) 122, 548
BsmI GAATGC 1 cut(s) 617
BsnI GGCC 4 cut(s) 772, 806, 1269, 1295
BsoBI CYCGRG 3 cut(s) 98, 709, 1253
Bsp119I TTCGAA 1 cut(s) 884
Bsp1286I GDGCHC 2 cut(s) 99, 663
Bsp13I TCCGGA 1 cut(s) 217
Bsp143I GATC 5 cut(s) 699, 747, 1107, 1249, 1333
BspACI CCGC 1 cut(s) 254
BspANI GGCC 4 cut(s) 772, 806, 1269, 1295
BspEI TCCGGA 1 cut(s) 217
BspLI GGNNCC 2 cut(s) 937, 1294
BspPI GGATC 3 cut(s) 694, 1244, 1341
BspT104I TTCGAA 1 cut(s) 884
BsrFI RCCGGY 1 cut(s) 1159
BsrI ACTGG 1 cut(s) 36
BssAI RCCGGY 1 cut(s) 1159
BssECI CCNNGG 4 cut(s) 329, 792, 962, 1345
BssMI GATC 5 cut(s) 699, 747, 1107, 1249, 1333
BssNI GRCGYC 2 cut(s) 214, 1354
BssSI CACGAG 1 cut(s) 1240
BssT1I CCWWGG 1 cut(s) 962
Bst2BI CACGAG 1 cut(s) 1240
Bst2UI CCWGG 1 cut(s) 987
Bst4CI ACNGT 1 cut(s) 567
Bst6I CTCTTC 1 cut(s) 31
BstACI GRCGYC 2 cut(s) 214, 1354
BstAPI GCANNNNNTGC 1 cut(s) 1284
BstBI TTCGAA 1 cut(s) 884
BstC8I GCNNGC 1 cut(s) 1161
BstDEI CTNAG 5 cut(s) 151, 530, 1194, 1228, 1365
BstENI CCTNNNNNAGG 1 cut(s) 89
BstF5I GGATG 2 cut(s) 393, 483
BstHHI GCGC 3 cut(s) 484, 517, 617
BstKTI GATC 5 cut(s) 702, 750, 1110, 1252, 1336
BstMAI GTCTC 4 cut(s) 127, 368, 712, 1129
BstMBI GATC 5 cut(s) 699, 747, 1107, 1249, 1333
BstMWI GCNNNNNNNGC 4 cut(s) 220, 521, 1275, 1284
BstNI CCWGG 1 cut(s) 987
BstSCI CCNGG 2 cut(s) 162, 985
BstV1I GCAGC 3 cut(s) 238, 286, 864
BstV2I GAAGAC 2 cut(s) 458, 902
BstX2I RGATCY 1 cut(s) 699
BstXI CCANNNNNNTGG 1 cut(s) 643
BstYI RGATCY 1 cut(s) 699
Bsu36I CCTNAGG 1 cut(s) 530
BsuRI GGCC 4 cut(s) 772, 806, 1269, 1295
BtsCI GGATG 2 cut(s) 393, 483
BtsI GCAGTG 1 cut(s) 1081
BtsIMutI CAGTG 2 cut(s) 563, 1081
Cac8I GCNNGC 1 cut(s) 1161
CfoI GCGC 3 cut(s) 484, 517, 617
Cfr10I RCCGGY 1 cut(s) 1159
Cfr13I GGNCC 5 cut(s) 197, 770, 899, 935, 1293
CseI GACGC 2 cut(s) 203, 1362
Csp6I GTAC 4 cut(s) 172, 241, 355, 739
CviAII CATG 3 cut(s) 497, 637, 745
CviQI GTAC 4 cut(s) 172, 241, 355, 739
DdeI CTNAG 5 cut(s) 151, 530, 1194, 1228, 1365
DpnI GATC 5 cut(s) 701, 749, 1109, 1251, 1335
DpnII GATC 5 cut(s) 699, 747, 1107, 1249, 1333
Eam1104I CTCTTC 1 cut(s) 31
EarI CTCTTC 1 cut(s) 31
Ecl136II GAGCTC 2 cut(s) 97, 661
Eco130I CCWWGG 1 cut(s) 962
Eco24I GRGCYC 2 cut(s) 99, 663
Eco47I GGWCC 3 cut(s) 197, 899, 935
Eco53kI GAGCTC 2 cut(s) 97, 661
Eco81I CCTNAGG 1 cut(s) 530
Eco88I CYCGRG 3 cut(s) 98, 709, 1253
EcoICRI GAGCTC 2 cut(s) 97, 661
EcoNI CCTNNNNNAGG 1 cut(s) 89
EcoRII CCWGG 1 cut(s) 985
EcoT14I CCWWGG 1 cut(s) 962
EcoT38I GRGCYC 2 cut(s) 99, 663
ErhI CCWWGG 1 cut(s) 962
FaeI CATG 3 cut(s) 500, 640, 748
FaiI YATR 8 cut(s) 228, 435, 498, 638, 644, 746, 871, 1338
FalI AAGNNNNNCTT 2 cut(s) 454, 486
FaqI GGGAC 2 cut(s) 122, 548
FatI CATG 3 cut(s) 496, 636, 744
FbaI TGATCA 1 cut(s) 747
Fnu4HI GCNGC 4 cut(s) 252, 255, 275, 878
FokI GGATG 2 cut(s) 400, 490
FriOI GRGCYC 2 cut(s) 99, 663
Fsp4HI GCNGC 4 cut(s) 252, 255, 275, 878
FspBI CTAG 3 cut(s) 86, 156, 1224
GlaI GCGC 3 cut(s) 483, 516, 616
GluI GCNGC 4 cut(s) 252, 255, 275, 878
GsaI CCCAGC 1 cut(s) 771
GsuI CTGGAG 1 cut(s) 881
HaeIII GGCC 4 cut(s) 772, 806, 1269, 1295
HapII CCGG 4 cut(s) 164, 218, 1143, 1160
HgaI GACGC 2 cut(s) 203, 1362
HhaI GCGC 3 cut(s) 484, 517, 617
Hin1I GRCGYC 2 cut(s) 214, 1354
Hin1II CATG 3 cut(s) 500, 640, 748
Hin6I GCGC 3 cut(s) 482, 515, 615
HinP1I GCGC 3 cut(s) 482, 515, 615
HincII GTYRAC 2 cut(s) 307, 493
HindII GTYRAC 2 cut(s) 307, 493
HinfI GANTC 5 cut(s) 67, 167, 334, 553, 1016
HpaII CCGG 4 cut(s) 164, 218, 1143, 1160
HphI GGTGA 8 cut(s) 76, 122, 383, 553, 657, 742, 958, 1259
Hpy166II GTNNAC 4 cut(s) 174, 307, 493, 1088
Hpy188I TCNGA 4 cut(s) 778, 832, 915, 1129
Hpy188III TCNNGA 9 cut(s) 182, 218, 673, 709, 860, 933, 1013, 1216, 1253
Hpy8I GTNNAC 4 cut(s) 174, 307, 493, 1088
Hpy99I CGWCG 2 cut(s) 623, 778
HpyAV CCTTC 1 cut(s) 992
HpyCH4III ACNGT 1 cut(s) 567
HpyCH4IV ACGT 3 cut(s) 135, 605, 907
HpyCH4V TGCA 5 cut(s) 191, 500, 654, 694, 1287
HpyF10VI GCNNNNNNNGC 4 cut(s) 220, 521, 1275, 1284
HpyF3I CTNAG 5 cut(s) 151, 530, 1194, 1228, 1365
HpySE526I ACGT 3 cut(s) 135, 605, 907
Hsp92I GRCGYC 2 cut(s) 214, 1354
Hsp92II CATG 3 cut(s) 500, 640, 748
HspAI GCGC 3 cut(s) 482, 515, 615
Kpn2I TCCGGA 1 cut(s) 217
KroI GCCGGC 1 cut(s) 1159
KroNI GCCGGC 1 cut(s) 1161
Ksp22I TGATCA 1 cut(s) 747
Kzo9I GATC 5 cut(s) 699, 747, 1107, 1249, 1333
LmnI GCTCC 3 cut(s) 94, 220, 282
Lsp1109I GCAGC 3 cut(s) 238, 286, 864
LweI GCATC 7 cut(s) 391, 526, 534, 1180, 1188, 1287, 1296
MaeI CTAG 3 cut(s) 86, 156, 1224
MaeII ACGT 3 cut(s) 135, 605, 907
MaeIII GTNAC 3 cut(s) 158, 344, 719
MalI GATC 5 cut(s) 701, 749, 1109, 1251, 1335
MboI GATC 5 cut(s) 699, 747, 1107, 1249, 1333
MboII GAAGA 7 cut(s) 18, 116, 462, 463, 709, 902, 1070
MfeI CAATTG 1 cut(s) 311
MflI RGATCY 1 cut(s) 699
MhlI GDGCHC 2 cut(s) 99, 663
MluCI AATT 8 cut(s) 290, 311, 590, 841, 854, 921, 956, 1066
MlyI GAGTC 1 cut(s) 1025
MmeI TCCRAC 5 cut(s) 37, 66, 588, 756, 1002
MroI TCCGGA 1 cut(s) 217
MroNI GCCGGC 1 cut(s) 1159
MseI TTAA 1 cut(s) 366
MslI CAYNNNNRTG 1 cut(s) 641
MspI CCGG 4 cut(s) 164, 218, 1143, 1160
MspR9I CCNGG 2 cut(s) 164, 987
MunI CAATTG 1 cut(s) 311
Mva1269I GAATGC 1 cut(s) 617
MvaI CCWGG 1 cut(s) 987
MwoI GCNNNNNNNGC 4 cut(s) 220, 521, 1275, 1284
NaeI GCCGGC 1 cut(s) 1161
NciI CCSGG 1 cut(s) 164
NdeII GATC 5 cut(s) 699, 747, 1107, 1249, 1333
NgoMIV GCCGGC 1 cut(s) 1159
NlaIII CATG 3 cut(s) 500, 640, 748
NlaIV GGNNCC 2 cut(s) 937, 1294
NmeAIII GCCGAG 2 cut(s) 354, 817
NmuCI GTSAC 2 cut(s) 158, 719
NspV TTCGAA 1 cut(s) 884
PaeR7I CTCGAG 2 cut(s) 98, 709
PctI GAATGC 1 cut(s) 617
PdiI GCCGGC 1 cut(s) 1161
PfeI GAWTC 4 cut(s) 67, 167, 334, 553
PfoI TCCNGGA 1 cut(s) 985
PkrI GCNGC 4 cut(s) 253, 256, 276, 879
PleI GAGTC 1 cut(s) 1024
PpsI GAGTC 1 cut(s) 1024
Psp124BI GAGCTC 2 cut(s) 99, 663
Psp6I CCWGG 1 cut(s) 985
PspFI CCCAGC 1 cut(s) 767
PspGI CCWGG 1 cut(s) 985
PspN4I GGNNCC 2 cut(s) 937, 1294
PspPI GGNCC 5 cut(s) 197, 770, 899, 935, 1293
PspXI VCTCGAGB 1 cut(s) 98
PsuI RGATCY 1 cut(s) 699
RsaI GTAC 4 cut(s) 173, 242, 356, 740
RsaNI GTAC 4 cut(s) 172, 241, 355, 739
RseI CAYNNNNRTG 1 cut(s) 641
SacI GAGCTC 2 cut(s) 99, 663
SaqAI TTAA 1 cut(s) 366
SatI GCNGC 4 cut(s) 252, 255, 275, 878
Sau3AI GATC 5 cut(s) 699, 747, 1107, 1249, 1333
Sau96I GGNCC 5 cut(s) 197, 770, 899, 935, 1293
ScaI AGTACT 1 cut(s) 356
SchI GAGTC 1 cut(s) 1025
ScrFI CCNGG 2 cut(s) 164, 987
SduI GDGCHC 2 cut(s) 99, 663
SfaNI GCATC 7 cut(s) 391, 526, 534, 1180, 1188, 1287, 1296
Sfr274I CTCGAG 2 cut(s) 98, 709
SfuI TTCGAA 1 cut(s) 884
SinI GGWCC 3 cut(s) 197, 899, 935
SlaI CTCGAG 2 cut(s) 98, 709
SmiMI CAYNNNNRTG 1 cut(s) 641
SmlI CTYRAG 5 cut(s) 98, 180, 548, 709, 1013
SmoI CTYRAG 5 cut(s) 98, 180, 548, 709, 1013
Sse9I AATT 8 cut(s) 290, 311, 590, 841, 854, 921, 956, 1066
SsiI CCGC 1 cut(s) 254
SspMI CTAG 3 cut(s) 86, 156, 1224
SstI GAGCTC 2 cut(s) 99, 663
StyD4I CCNGG 2 cut(s) 162, 985
StyI CCWWGG 1 cut(s) 962
TaaI ACNGT 1 cut(s) 567
TaiI ACGT 3 cut(s) 138, 608, 910
TaqI TCGA 8 cut(s) 99, 324, 710, 839, 884, 1070, 1175, 1188
TaqII GACCGA 1 cut(s) 185
TasI AATT 8 cut(s) 290, 311, 590, 841, 854, 921, 956, 1066
TatI WGTACW 1 cut(s) 354
TauI GCSGC 1 cut(s) 257
TfiI GAWTC 4 cut(s) 67, 167, 334, 553
Tru1I TTAA 1 cut(s) 366
Tru9I TTAA 1 cut(s) 366
TscAI CASTG 2 cut(s) 570, 1081
TseFI GTSAC 2 cut(s) 158, 719
TseI GCWGC 3 cut(s) 251, 274, 877
Tsp45I GTSAC 2 cut(s) 158, 719
TspDTI ATGAA 3 cut(s) 692, 1077, 1341
TspRI CASTG 2 cut(s) 570, 1081
VpaK11BI GGWCC 3 cut(s) 197, 899, 935
XagI CCTNNNNNAGG 1 cut(s) 89
XapI RAATTY 5 cut(s) 290, 590, 841, 921, 1066
XhoI CTCGAG 2 cut(s) 98, 709
XspI CTAG 3 cut(s) 86, 156, 1224
ZrmI AGTACT 1 cut(s) 356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.