RchiOBHm_Chr5g0045921

UPF0481 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000833
Physical Location & Seq
Reverse (-)
41978329 .. 41979561
1233 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32397

Sequence Viewer

Length: 1233 bp
ATGATGGCCATTGATGCTTCGTTTTTCCTCGAAATCCTCCAAGTCTATGAGGTCCAAGAAGGCCCAAGAGTTTCTTCAAGGATGGCGCATTTGGTTGACCATGCAGGAAGGAAGTCAGCGCATCATGCAATCCTTAGGGATTTGGTGATGCTTGAGAACCAAATCCCACGATTTGTTTTGAAAAAAGTGTTGAAGTTTAAGCTCCCAGCGTTGGAATGTGCCAAAGATATGCTTCTTTCCATGTTTATGGGATTGTGCAATGAGCTCTCCCCATTCAAGATGAATGACGAAGACATGCCAAATATGGGAGTGACTATCCAATTCTCGAGTTGTTTTAACTTGCTGGACTCCTTGTACCGCCTGATCACCCCCAAGTTAGTAGCCGAGCCATCGGAAATCACAGAAGCCGTGGGATATGAGGCCGCACAACAACAGAAGGGAAAATCATCAGTTAATTCGAATTTCGTCAAACAATTTCTGAAGCAGGTACGGAAGCTGCTATCGAAGTTGAATAAAGGCCCAATACCTCTTCTGAGAAAAATTCCAATATATGGACCTGTAAAAGTGATATCCGACCTGCCTTGGACTATCCTCTTTGACCTTCTTTCCACCCAAGACAAAGAAGCAGTCAAACCCGAAGATGACAGTTCGAGCAATGGCATTAGTAAACCGCCGAAGATAGAGGAGATCACCATCCCATCAGTCTCCGATCTCTCAAAATCTGGTGTCAAGTTCTTGGCCGGTGGAATTAGAATTTCAGACATCTCTTTCAATGCTGAAACTGGCTCATTTACCCTCCCGATTACTAGCTTAGATGGGAACACAGGGGTGATCCTGAGAAACTTGGTGGCCTATGAAGCATCAAGTGTATCGGGGCCATTGGTTTTCACTCGCTACACCGGGTTGATGAACGGGATCATAGACACCGAGGAGGATGTCAAGTTGCTTAGAGAGAAGCGTATCATCATGAACCATTTGAAGAGTGATGAAGAAGTGGCAAATCTGCTCAATGGCATGAGCAGGTCCATTAGATTGACCAAAGTGCCCTCCTTGGATAAGGTAATCGCAGATGCCAATGAGTATTACAATGGTAGATGGAAAGTGAAGATGAGGAAGTTTTTGAACACTTTTGTGTATGGTTCATGGCCGTTACTGGCTTTCCTCGCTGCCATTTTGCTCCTGCTCTTGATGACATTGCAAGCAGTCTGCTCAGTCTATAGGTGCAGTACGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

410

Amino Acids

45.79

Weight (kDa)

8.88

Isoelectric Point (pI)

33.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 1 - 396 3e-69 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 475, 585, 1011
AccB7I CCANNNNNTGG 1 cut(s) 551
AciI CCGC 3 cut(s) 358, 423, 671
AclWI GGATC 2 cut(s) 826, 923
AcoI YGGCCR 3 cut(s) 6, 738, 1145
AcsI RAATTY 3 cut(s) 460, 540, 753
AcuI CTGAAG 1 cut(s) 500
AfaI GTAC 3 cut(s) 356, 489, 1228
AfiI CCNNNNNNNGG 3 cut(s) 211, 305, 551
AgsI TTSAA 8 cut(s) 78, 181, 193, 277, 511, 772, 979, 1123
AjuI GAANNNNNNNTTGG 2 cut(s) 58, 90
AleI CACNNNNGTG 2 cut(s) 827, 1130
AluBI AGCT 4 cut(s) 202, 265, 496, 810
AluI AGCT 4 cut(s) 202, 265, 496, 810
Alw21I GWGCWC 1 cut(s) 267
Alw26I GTCTC 1 cut(s) 709
AlwI GGATC 2 cut(s) 826, 923
Ama87I CYCGRG 1 cut(s) 325
AoxI GGCC 8 cut(s) 6, 61, 420, 517, 738, 849, 875, 1145
ApeKI GCWGC 2 cut(s) 496, 1166
ApoI RAATTY 3 cut(s) 460, 540, 753
ArsI GACNNNNNNTTYG 2 cut(s) 612, 644
AspLEI GCGC 2 cut(s) 88, 121
AspS9I GGNCC 6 cut(s) 52, 62, 518, 554, 875, 1023
AsuC2I CCSGG 1 cut(s) 901
AsuHPI GGTGA 4 cut(s) 157, 358, 682, 841
AsuII TTCGAA 1 cut(s) 458
AvaI CYCGRG 1 cut(s) 325
AvaII GGWCC 3 cut(s) 52, 554, 1023
AxyI CCTNAGG 1 cut(s) 134
BaeGI GKGCMC 1 cut(s) 1047
BalI TGGCCA 1 cut(s) 8
BanII GRGCYC 1 cut(s) 267
BbsI GAAGAC 1 cut(s) 297
Bbv12I GWGCWC 1 cut(s) 267
BbvI GCAGC 2 cut(s) 483, 1153
BccI CCATC 6 cut(s) 76, 397, 701, 706, 809, 1089
BceAI ACGGC 2 cut(s) 392, 1132
BclI TGATCA 1 cut(s) 363
BcnI CCSGG 1 cut(s) 901
BcoDI GTCTC 1 cut(s) 709
BfaI CTAG 1 cut(s) 807
BfmI CTRYAG 1 cut(s) 1216
BfuAI ACCTGC 3 cut(s) 475, 585, 1011
BisI GCNGC 3 cut(s) 423, 497, 1167
BlsI GCNGC 3 cut(s) 424, 498, 1168
Bme1390I CCNGG 1 cut(s) 901
Bme18I GGWCC 3 cut(s) 52, 554, 1023
BmeT110I CYCGRG 1 cut(s) 325
BmgT120I GGNCC 6 cut(s) 52, 62, 518, 554, 875, 1023
BmiI GGNNCC 1 cut(s) 876
BmrFI CCNGG 1 cut(s) 901
BmsI GCATC 5 cut(s) 4, 130, 138, 869, 1060
BpiI GAAGAC 1 cut(s) 297
Bpu14I TTCGAA 1 cut(s) 458
BpuEI CTTGAG 1 cut(s) 173
BpuMI CCSGG 1 cut(s) 901
BsaAI YACGTR 1 cut(s) 1230
BsaBI GATNNNNATC 1 cut(s) 692
BsaJI CCNNGG 4 cut(s) 408, 581, 927, 1050
Bsc4I CCNNNNNNNGG 3 cut(s) 211, 305, 551
Bse118I RCCGGY 1 cut(s) 740
Bse1I ACTGG 2 cut(s) 787, 1158
Bse21I CCTNAGG 1 cut(s) 134
Bse3DI GCAATG 3 cut(s) 265, 661, 1193
Bse8I GATNNNNATC 1 cut(s) 692
BseDI CCNNGG 4 cut(s) 408, 581, 927, 1050
BseGI GGATG 3 cut(s) 87, 693, 940
BseJI GATNNNNATC 1 cut(s) 692
BseLI CCNNNNNNNGG 3 cut(s) 211, 305, 551
BseMI GCAATG 3 cut(s) 265, 661, 1193
BseMII CTCAG 3 cut(s) 524, 827, 1224
BseNI ACTGG 2 cut(s) 787, 1158
BseRI GAGGAG 2 cut(s) 698, 944
BseSI GKGCMC 1 cut(s) 1047
BseXI GCAGC 2 cut(s) 483, 1153
BseYI CCCAGC 1 cut(s) 205
BshFI GGCC 8 cut(s) 8, 63, 422, 519, 740, 851, 877, 1147
BsiHKAI GWGCWC 1 cut(s) 267
BsiHKCI CYCGRG 1 cut(s) 325
BsiSI CCGG 2 cut(s) 741, 900
BslI CCNNNNNNNGG 3 cut(s) 211, 305, 551
BsmAI GTCTC 1 cut(s) 709
BsnI GGCC 8 cut(s) 8, 63, 422, 519, 740, 851, 877, 1147
BsoBI CYCGRG 1 cut(s) 325
Bsp119I TTCGAA 1 cut(s) 458
Bsp1286I GDGCHC 2 cut(s) 267, 1047
Bsp143I GATC 5 cut(s) 363, 687, 709, 831, 915
BspACI CCGC 3 cut(s) 358, 423, 671
BspANI GGCC 8 cut(s) 8, 63, 422, 519, 740, 851, 877, 1147
BspCNI CTCAG 3 cut(s) 525, 828, 1223
BspHI TCATGA 1 cut(s) 966
BspLI GGNNCC 1 cut(s) 876
BspMI ACCTGC 3 cut(s) 475, 585, 1011
BspPI GGATC 2 cut(s) 826, 923
BspT104I TTCGAA 1 cut(s) 458
BsrDI GCAATG 3 cut(s) 265, 661, 1193
BsrFI RCCGGY 1 cut(s) 740
BsrI ACTGG 2 cut(s) 787, 1158
BssAI RCCGGY 1 cut(s) 740
BssECI CCNNGG 4 cut(s) 408, 581, 927, 1050
BssMI GATC 5 cut(s) 363, 687, 709, 831, 915
BssT1I CCWWGG 2 cut(s) 581, 1050
Bst4CI ACNGT 1 cut(s) 647
Bst6I CTCTTC 2 cut(s) 534, 974
BstBAI YACGTR 1 cut(s) 1230
BstBI TTCGAA 1 cut(s) 458
BstC8I GCNNGC 1 cut(s) 1200
BstDEI CTNAG 6 cut(s) 134, 533, 811, 836, 947, 1210
BstDSI CCRYGG 1 cut(s) 408
BstF5I GGATG 3 cut(s) 87, 693, 940
BstHHI GCGC 2 cut(s) 88, 121
BstKTI GATC 5 cut(s) 366, 690, 712, 834, 918
BstMAI GTCTC 1 cut(s) 709
BstMBI GATC 5 cut(s) 363, 687, 709, 831, 915
BstMWI GCNNNNNNNGC 4 cut(s) 14, 125, 857, 1163
BstNSI RCATGY 1 cut(s) 298
BstSCI CCNGG 1 cut(s) 899
BstSFI CTRYAG 1 cut(s) 1216
BstSLI GKGCMC 1 cut(s) 1047
BstSNI TACGTA 1 cut(s) 1230
BstV1I GCAGC 2 cut(s) 483, 1153
BstV2I GAAGAC 1 cut(s) 297
BstXI CCANNNNNNTGG 1 cut(s) 247
Bsu36I CCTNAGG 1 cut(s) 134
BsuRI GGCC 8 cut(s) 8, 63, 422, 519, 740, 851, 877, 1147
BtgI CCRYGG 1 cut(s) 408
BtsCI GGATG 3 cut(s) 87, 693, 940
BveI ACCTGC 3 cut(s) 475, 585, 1011
Cac8I GCNNGC 1 cut(s) 1200
CciI TCATGA 1 cut(s) 966
CfoI GCGC 2 cut(s) 88, 121
Cfr10I RCCGGY 1 cut(s) 740
Cfr13I GGNCC 6 cut(s) 52, 62, 518, 554, 875, 1023
Csp6I GTAC 3 cut(s) 355, 488, 1227
CviAII CATG 7 cut(s) 101, 125, 241, 295, 967, 1015, 1143
CviQI GTAC 3 cut(s) 355, 488, 1227
DdeI CTNAG 6 cut(s) 134, 533, 811, 836, 947, 1210
DpnI GATC 5 cut(s) 365, 689, 711, 833, 917
DpnII GATC 5 cut(s) 363, 687, 709, 831, 915
EaeI YGGCCR 3 cut(s) 6, 738, 1145
Eam1104I CTCTTC 2 cut(s) 534, 974
EarI CTCTTC 2 cut(s) 534, 974
Ecl136II GAGCTC 1 cut(s) 265
Eco105I TACGTA 1 cut(s) 1230
Eco130I CCWWGG 2 cut(s) 581, 1050
Eco24I GRGCYC 1 cut(s) 267
Eco32I GATATC 1 cut(s) 570
Eco47I GGWCC 3 cut(s) 52, 554, 1023
Eco53kI GAGCTC 1 cut(s) 265
Eco57I CTGAAG 1 cut(s) 500
Eco81I CCTNAGG 1 cut(s) 134
Eco88I CYCGRG 1 cut(s) 325
EcoICRI GAGCTC 1 cut(s) 265
EcoRV GATATC 1 cut(s) 570
EcoT14I CCWWGG 2 cut(s) 581, 1050
EcoT38I GRGCYC 1 cut(s) 267
ErhI CCWWGG 2 cut(s) 581, 1050
FaeI CATG 7 cut(s) 104, 128, 244, 298, 970, 1018, 1146
FalI AAGNNNNNCTT 4 cut(s) 58, 90, 216, 248
FatI CATG 7 cut(s) 100, 124, 240, 294, 966, 1014, 1142
FbaI TGATCA 1 cut(s) 363
Fnu4HI GCNGC 3 cut(s) 423, 497, 1167
FokI GGATG 3 cut(s) 94, 680, 947
FriOI GRGCYC 1 cut(s) 267
Fsp4HI GCNGC 3 cut(s) 423, 497, 1167
FspBI CTAG 1 cut(s) 807
GlaI GCGC 2 cut(s) 87, 120
GluI GCNGC 3 cut(s) 423, 497, 1167
GsaI CCCAGC 1 cut(s) 209
HaeIII GGCC 8 cut(s) 8, 63, 422, 519, 740, 851, 877, 1147
HapII CCGG 2 cut(s) 741, 900
HhaI GCGC 2 cut(s) 88, 121
Hin1II CATG 7 cut(s) 104, 128, 244, 298, 970, 1018, 1146
Hin6I GCGC 2 cut(s) 86, 119
HinP1I GCGC 2 cut(s) 86, 119
HincII GTYRAC 1 cut(s) 97
HindII GTYRAC 1 cut(s) 97
HinfI GANTC 1 cut(s) 347
HpaII CCGG 2 cut(s) 741, 900
HphI GGTGA 4 cut(s) 157, 358, 682, 841
Hpy166II GTNNAC 2 cut(s) 97, 668
Hpy188I TCNGA 6 cut(s) 394, 480, 534, 574, 709, 760
Hpy188III TCNNGA 6 cut(s) 277, 325, 799, 835, 967, 1186
Hpy8I GTNNAC 2 cut(s) 97, 668
HpyAV CCTTC 4 cut(s) 53, 102, 430, 611
HpyCH4III ACNGT 1 cut(s) 647
HpyCH4IV ACGT 1 cut(s) 1229
HpyCH4V TGCA 5 cut(s) 104, 128, 258, 1198, 1224
HpyF10VI GCNNNNNNNGC 4 cut(s) 14, 125, 857, 1163
HpyF3I CTNAG 6 cut(s) 134, 533, 811, 836, 947, 1210
HpySE526I ACGT 1 cut(s) 1229
Hsp92II CATG 7 cut(s) 104, 128, 244, 298, 970, 1018, 1146
HspAI GCGC 2 cut(s) 86, 119
Ksp22I TGATCA 1 cut(s) 363
Kzo9I GATC 5 cut(s) 363, 687, 709, 831, 915
LmnI GCTCC 2 cut(s) 207, 1182
Lsp1109I GCAGC 2 cut(s) 483, 1153
LweI GCATC 5 cut(s) 4, 130, 138, 869, 1060
MaeI CTAG 1 cut(s) 807
MaeII ACGT 1 cut(s) 1229
MaeIII GTNAC 2 cut(s) 310, 1149
MalI GATC 5 cut(s) 365, 689, 711, 833, 917
MboI GATC 5 cut(s) 363, 687, 709, 831, 915
MboII GAAGA 8 cut(s) 66, 302, 521, 650, 688, 991, 1001, 1117
MhlI GDGCHC 2 cut(s) 267, 1047
MlsI TGGCCA 1 cut(s) 8
MluCI AATT 7 cut(s) 320, 454, 460, 473, 540, 747, 753
MluNI TGGCCA 1 cut(s) 8
MlyI GAGTC 1 cut(s) 341
MmeI TCCRAC 2 cut(s) 192, 597
Mox20I TGGCCA 1 cut(s) 8
MscI TGGCCA 1 cut(s) 8
MseI TTAA 3 cut(s) 198, 336, 453
MslI CAYNNNNRTG 3 cut(s) 245, 827, 1130
Msp20I TGGCCA 1 cut(s) 8
MspI CCGG 2 cut(s) 741, 900
MspR9I CCNGG 1 cut(s) 901
MwoI GCNNNNNNNGC 4 cut(s) 14, 125, 857, 1163
NciI CCSGG 1 cut(s) 901
NdeII GATC 5 cut(s) 363, 687, 709, 831, 915
NlaIII CATG 7 cut(s) 104, 128, 244, 298, 970, 1018, 1146
NlaIV GGNNCC 1 cut(s) 876
NmeAIII GCCGAG 1 cut(s) 409
NmuCI GTSAC 1 cut(s) 310
NspI RCATGY 1 cut(s) 298
NspV TTCGAA 1 cut(s) 458
OliI CACNNNNGTG 2 cut(s) 827, 1130
PaeR7I CTCGAG 1 cut(s) 325
PagI TCATGA 1 cut(s) 966
PflMI CCANNNNNTGG 1 cut(s) 551
PkrI GCNGC 3 cut(s) 424, 498, 1168
PleI GAGTC 1 cut(s) 341
PpsI GAGTC 1 cut(s) 341
Ppu21I YACGTR 1 cut(s) 1230
Psp124BI GAGCTC 1 cut(s) 267
PspFI CCCAGC 1 cut(s) 205
PspN4I GGNNCC 1 cut(s) 876
PspPI GGNCC 6 cut(s) 52, 62, 518, 554, 875, 1023
RsaI GTAC 3 cut(s) 356, 489, 1228
RsaNI GTAC 3 cut(s) 355, 488, 1227
RseI CAYNNNNRTG 3 cut(s) 245, 827, 1130
SacI GAGCTC 1 cut(s) 267
SaqAI TTAA 3 cut(s) 198, 336, 453
SatI GCNGC 3 cut(s) 423, 497, 1167
Sau3AI GATC 5 cut(s) 363, 687, 709, 831, 915
Sau96I GGNCC 6 cut(s) 52, 62, 518, 554, 875, 1023
SchI GAGTC 1 cut(s) 341
ScrFI CCNGG 1 cut(s) 901
SduI GDGCHC 2 cut(s) 267, 1047
SfaNI GCATC 5 cut(s) 4, 130, 138, 869, 1060
SfcI CTRYAG 1 cut(s) 1216
Sfr274I CTCGAG 1 cut(s) 325
SfuI TTCGAA 1 cut(s) 458
SinI GGWCC 3 cut(s) 52, 554, 1023
SlaI CTCGAG 1 cut(s) 325
SmiMI CAYNNNNRTG 3 cut(s) 245, 827, 1130
SmlI CTYRAG 2 cut(s) 152, 325
SmoI CTYRAG 2 cut(s) 152, 325
SnaBI TACGTA 1 cut(s) 1230
Sse9I AATT 7 cut(s) 320, 454, 460, 473, 540, 747, 753
SsiI CCGC 3 cut(s) 358, 423, 671
SspMI CTAG 1 cut(s) 807
SstI GAGCTC 1 cut(s) 267
StyD4I CCNGG 1 cut(s) 899
StyI CCWWGG 2 cut(s) 581, 1050
TaaI ACNGT 1 cut(s) 647
TaiI ACGT 1 cut(s) 1232
TaqI TCGA 5 cut(s) 30, 326, 458, 503, 650
TasI AATT 7 cut(s) 320, 454, 460, 473, 540, 747, 753
TauI GCSGC 1 cut(s) 425
Tru1I TTAA 3 cut(s) 198, 336, 453
Tru9I TTAA 3 cut(s) 198, 336, 453
TseFI GTSAC 1 cut(s) 310
TseI GCWGC 2 cut(s) 496, 1166
Tsp45I GTSAC 1 cut(s) 310
TspDTI ATGAA 6 cut(s) 296, 870, 923, 983, 1002, 1131
TspGWI ACGGA 1 cut(s) 505
Van91I CCANNNNNTGG 1 cut(s) 551
VpaK11BI GGWCC 3 cut(s) 52, 554, 1023
XapI RAATTY 3 cut(s) 460, 540, 753
XceI RCATGY 1 cut(s) 298
XhoI CTCGAG 1 cut(s) 325
XspI CTAG 1 cut(s) 807
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.