Rh6AG014100

UPF0481 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0000833
Physical Location & Seq
Forward (+)
1483995 .. 1486215
2221 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG014100.1

Sequence Viewer

Length: 1665 bp
ATGTCTTCTCTCCAACCAACATTCTCTTCCAATTCTAGTTCAAACTTCAATGAGCTTCAATGGGTGATTCAAATCCAAAAATTCCTAGAGGAAGAGTTCGAGCATGATATTGAAATCCCTATCTCCATTTTCAACGTCCCCAAATCCCTCATGGCTAGTGACCCAGAATCGTACACCCCTCAAGTGGTTGCGATTGGTCCTTACCATCATTGGCGTCCGGAGCTGTATGAGATGGTGAGGTATAAAGTGGCTGCAGCTAAAAGAACCCAAAAGCAGCTCCAAACCCTCAAATTCCAGGACCTTGTTGACCAATTGACAAGGTTCGAGCTGAGGATTCGGGCTTGTTACCACAAGTACCTGGAGTTTAATGGCGAGACATTGGGGTGGATGATGGCTATTGATGCTTCGTTTTTCTTCGAAATCCTCCAAGTCTATGTAGTCCAAGAAGGGAACACATCGACGAGAGTTTCTTCAAGGATGGCACATTTGGTTGACTATGCAGGAAGGAAGTCAGCACATCATGCCATCCTTAGGGACTTGGTGATGCTTGAGAACCAAATGCCACTATTTGTTTTGAGAGAAGTGTTGGCATTTCAGTTACCAACGTTGGAATGCACCGACAATATGCTTCTTTCCATGTTTATGGGACTGTGCAAAGAGCTCTCCCCATTCAAGATGAATGATGAAGATATGCCAAAGATCCAAGTGTCGAGTTGTTGTCACTTGCTAGACTTTTTATACCGCATGATCACCCCCAAATTAGAAACTAGACCGTCAGAAATTACAGAAACCGAGGATGGTCCGGCCACACCACACAAGGGAAAGTCTTCAGATAACTCGAATTTCCTCAAACAATTTCTGGAGAAGGTAGGGAAGCTGCTATCGAAGTTGAACAAGGGCCCGATACGTCTTTTGAAAAAAATTCTAATTTCTCGACCTGTAAAAGTGTTACACAAACTGCCTTGGACTATCCTTTCCAACCATGCTGGACCCGCAATTTTGAAACAACCTCTCGAGTTACTTTCCTCCCAAGACAAAGAAGCAGTCAAACCTGAAGATGATAAATCAAGCAATAGACCGCCGTTGATAGAGGAGATCACCATCCCATCAGTCTCCAAACTCTCAAAATCAGGTGTCAAGTTCTTGCCCGCAAGTAGTATTTCAACCATCTCTTTCGATGCTAAAACTTGCTCATTTTACCTCCCGAGTACAAGCTTAGATGGGAACACAGAGGTGATCCTGAGAAACTTGGTGGCCTACGAGGCATCAACTGCATCGGGGCCATTAGTTTTTACTCGCTACACCGAGTTGATGAACGGAATCATAGACACCGAGGAAGACGCCAAGTTGCTTAGAGAGAAGGGTATCATCGTGAATCGTTTGAAGAGTGATGAAGAAGTGGCAAAGCTGTTCAATGGCATGAGCAGGTCCATTAGATTGACCAAAGTGCCCTTCTTGGATAAGGCAATCAAAGATGCCAACAAGTATTACCATGGTAGATGGAAGGTGAAGATGAGGAAGTCTTTGAAAACTTATGTGTTCGGTTCTTGGCAATTTCTGGCTTCGCTCGCTACCATTTTTCTCCTGCTCTTGATGACATTGGAAGCATTTTGCTCAGTCTATAGCTGCAGTCGCATGTTTCATATCAACACCACTAATAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

554

Amino Acids

63.27

Weight (kDa)

8.83

Isoelectric Point (pI)

48.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 43 - 531 2.5e-104 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1416
AccIII TCCGGA 1 cut(s) 217
AciI CCGC 4 cut(s) 742, 993, 1079, 1149
AclI AACGTT 1 cut(s) 605
AclWI GGATC 2 cut(s) 694, 1231
AcoI YGGCCR 1 cut(s) 804
AcsI RAATTY 4 cut(s) 80, 290, 841, 921
AcuI CTGAAG 2 cut(s) 813, 1074
AcyI GRCGYC 2 cut(s) 214, 1341
AfaI GTAC 3 cut(s) 173, 356, 1210
AfiI CCNNNNNNNGG 3 cut(s) 184, 531, 818
AjnI CCWGG 2 cut(s) 294, 357
AjuI GAANNNNNNNTTGG 4 cut(s) 10, 42, 1436, 1468
AleI CACNNNNGTG 1 cut(s) 1232
Alw21I GWGCWC 1 cut(s) 663
Alw26I GTCTC 2 cut(s) 368, 1117
AlwI GGATC 2 cut(s) 694, 1231
Ama87I CYCGRG 2 cut(s) 1013, 1204
Aor13HI TCCGGA 1 cut(s) 217
AoxI GGCC 4 cut(s) 804, 898, 1254, 1280
ApaI GGGCCC 1 cut(s) 902
ApeKI GCWGC 5 cut(s) 251, 254, 274, 877, 1626
ApoI RAATTY 4 cut(s) 80, 290, 841, 921
ArsI GACNNNNNNTTYG 2 cut(s) 1029, 1061
Asp700I GAANNNNTTC 1 cut(s) 826
AspS9I GGNCC 8 cut(s) 197, 298, 800, 898, 899, 989, 1280, 1428
AsuHPI GGTGA 7 cut(s) 76, 247, 553, 742, 1090, 1246, 1519
AsuII TTCGAA 1 cut(s) 417
AvaI CYCGRG 2 cut(s) 1013, 1204
AvaII GGWCC 5 cut(s) 197, 298, 800, 989, 1428
AxyI CCTNAGG 1 cut(s) 530
BaeGI GKGCMC 2 cut(s) 902, 1452
BanII GRGCYC 2 cut(s) 663, 902
BbsI GAAGAC 2 cut(s) 819, 1344
Bbv12I GWGCWC 1 cut(s) 663
BbvCI CCTCAGC 1 cut(s) 329
BbvI GCAGC 5 cut(s) 238, 266, 286, 864, 1613
BceAI ACGGC 1 cut(s) 1066
BciT130I CCWGG 2 cut(s) 296, 359
BclI TGATCA 1 cut(s) 747
BcoDI GTCTC 2 cut(s) 368, 1117
BfaI CTAG 5 cut(s) 36, 86, 156, 728, 768
BfmI CTRYAG 3 cut(s) 252, 1621, 1627
BfuAI ACCTGC 1 cut(s) 1416
BglI GCCNNNNNGGC 1 cut(s) 1262
BisI GCNGC 5 cut(s) 252, 255, 275, 878, 1627
BlsI GCNGC 5 cut(s) 253, 256, 276, 879, 1628
Bme1390I CCNGG 2 cut(s) 296, 359
Bme18I GGWCC 5 cut(s) 197, 298, 800, 989, 1428
BmeT110I CYCGRG 2 cut(s) 1013, 1204
BmgT120I GGNCC 8 cut(s) 197, 298, 800, 898, 899, 989, 1280, 1428
BmiI GGNNCC 3 cut(s) 900, 991, 1281
BmrFI CCNGG 2 cut(s) 296, 359
BmsI GCATC 6 cut(s) 391, 534, 1168, 1274, 1283, 1465
BpiI GAAGAC 2 cut(s) 819, 1344
BpmI CTGGAG 2 cut(s) 380, 881
Bpu10I CCTNAGC 1 cut(s) 329
Bpu14I TTCGAA 1 cut(s) 417
BpuEI CTTGAG 2 cut(s) 165, 569
BsaBI GATNNNNATC 2 cut(s) 71, 1100
BsaHI GRCGYC 2 cut(s) 214, 1341
BsaJI CCNNGG 4 cut(s) 792, 962, 1332, 1492
BsaWI WCCGGW 1 cut(s) 217
Bsc4I CCNNNNNNNGG 3 cut(s) 184, 531, 818
Bse21I CCTNAGG 1 cut(s) 530
Bse8I GATNNNNATC 2 cut(s) 71, 1100
BseAI TCCGGA 1 cut(s) 217
BseBI CCWGG 2 cut(s) 296, 359
BseDI CCNNGG 4 cut(s) 792, 962, 1332, 1492
BseGI GGATG 5 cut(s) 393, 483, 525, 802, 1101
BseJI GATNNNNATC 2 cut(s) 71, 1100
BseLI CCNNNNNNNGG 3 cut(s) 184, 531, 818
BseMII CTCAG 3 cut(s) 320, 1232, 1629
BseRI GAGGAG 1 cut(s) 1106
BseSI GKGCMC 2 cut(s) 902, 1452
BseXI GCAGC 5 cut(s) 238, 266, 286, 864, 1613
BshFI GGCC 4 cut(s) 806, 900, 1256, 1282
BsiHKAI GWGCWC 1 cut(s) 663
BsiHKCI CYCGRG 2 cut(s) 1013, 1204
BsiSI CCGG 2 cut(s) 218, 803
BslFI GGGAC 3 cut(s) 122, 548, 660
BslI CCNNNNNNNGG 3 cut(s) 184, 531, 818
BsmAI GTCTC 2 cut(s) 368, 1117
BsmFI GGGAC 3 cut(s) 122, 548, 660
BsmI GAATGC 1 cut(s) 617
BsnI GGCC 4 cut(s) 806, 900, 1256, 1282
BsoBI CYCGRG 2 cut(s) 1013, 1204
Bsp119I TTCGAA 1 cut(s) 417
Bsp120I GGGCCC 1 cut(s) 898
Bsp1286I GDGCHC 3 cut(s) 663, 902, 1452
Bsp13I TCCGGA 1 cut(s) 217
Bsp143I GATC 4 cut(s) 699, 747, 1095, 1236
Bsp19I CCATGG 1 cut(s) 1492
BspACI CCGC 4 cut(s) 742, 993, 1079, 1149
BspANI GGCC 4 cut(s) 806, 900, 1256, 1282
BspCNI CTCAG 3 cut(s) 321, 1233, 1628
BspEI TCCGGA 1 cut(s) 217
BspLI GGNNCC 3 cut(s) 900, 991, 1281
BspMAI CTGCAG 2 cut(s) 256, 1631
BspMI ACCTGC 1 cut(s) 1416
BspPI GGATC 2 cut(s) 694, 1231
BspT104I TTCGAA 1 cut(s) 417
BssECI CCNNGG 4 cut(s) 792, 962, 1332, 1492
BssMI GATC 4 cut(s) 699, 747, 1095, 1236
BssNI GRCGYC 2 cut(s) 214, 1341
BssT1I CCWWGG 2 cut(s) 962, 1492
Bst2UI CCWGG 2 cut(s) 296, 359
Bst4CI ACNGT 2 cut(s) 651, 774
Bst6I CTCTTC 3 cut(s) 31, 87, 1379
BstACI GRCGYC 2 cut(s) 214, 1341
BstAPI GCANNNNNTGC 2 cut(s) 521, 1271
BstBI TTCGAA 1 cut(s) 417
BstC8I GCNNGC 2 cut(s) 1149, 1569
BstDEI CTNAG 6 cut(s) 329, 530, 1216, 1241, 1352, 1615
BstDSI CCRYGG 1 cut(s) 1492
BstF5I GGATG 5 cut(s) 393, 483, 525, 802, 1101
BstKTI GATC 4 cut(s) 702, 750, 1098, 1239
BstMAI GTCTC 2 cut(s) 368, 1117
BstMBI GATC 4 cut(s) 699, 747, 1095, 1236
BstMWI GCNNNNNNNGC 8 cut(s) 220, 401, 521, 992, 1262, 1271, 1568, 1632
BstNI CCWGG 2 cut(s) 296, 359
BstNSI RCATGY 1 cut(s) 1639
BstSCI CCNGG 2 cut(s) 294, 357
BstSFI CTRYAG 3 cut(s) 252, 1621, 1627
BstSLI GKGCMC 2 cut(s) 902, 1452
BstV1I GCAGC 5 cut(s) 238, 266, 286, 864, 1613
BstV2I GAAGAC 2 cut(s) 819, 1344
BstX2I RGATCY 1 cut(s) 699
BstXI CCANNNNNNTGG 1 cut(s) 643
BstYI RGATCY 1 cut(s) 699
Bsu36I CCTNAGG 1 cut(s) 530
BsuRI GGCC 4 cut(s) 806, 900, 1256, 1282
BtgI CCRYGG 1 cut(s) 1492
BtsCI GGATG 5 cut(s) 393, 483, 525, 802, 1101
BveI ACCTGC 1 cut(s) 1416
Cac8I GCNNGC 2 cut(s) 1149, 1569
Cfr13I GGNCC 8 cut(s) 197, 298, 800, 898, 899, 989, 1280, 1428
CseI GACGC 2 cut(s) 203, 1349
Csp6I GTAC 3 cut(s) 172, 355, 1209
CviAII CATG 9 cut(s) 104, 151, 521, 637, 745, 983, 1420, 1493, 1636
CviQI GTAC 3 cut(s) 172, 355, 1209
DdeI CTNAG 6 cut(s) 329, 530, 1216, 1241, 1352, 1615
DpnI GATC 4 cut(s) 701, 749, 1097, 1238
DpnII GATC 4 cut(s) 699, 747, 1095, 1236
EaeI YGGCCR 1 cut(s) 804
Eam1104I CTCTTC 3 cut(s) 31, 87, 1379
EarI CTCTTC 3 cut(s) 31, 87, 1379
Ecl136II GAGCTC 1 cut(s) 661
Eco130I CCWWGG 2 cut(s) 962, 1492
Eco24I GRGCYC 2 cut(s) 663, 902
Eco47I GGWCC 5 cut(s) 197, 298, 800, 989, 1428
Eco53kI GAGCTC 1 cut(s) 661
Eco57I CTGAAG 2 cut(s) 813, 1074
Eco81I CCTNAGG 1 cut(s) 530
Eco88I CYCGRG 2 cut(s) 1013, 1204
EcoICRI GAGCTC 1 cut(s) 661
EcoO109I RGGNCCY 2 cut(s) 298, 898
EcoRII CCWGG 2 cut(s) 294, 357
EcoT14I CCWWGG 2 cut(s) 962, 1492
EcoT38I GRGCYC 2 cut(s) 663, 902
ErhI CCWWGG 2 cut(s) 962, 1492
FaeI CATG 9 cut(s) 107, 154, 524, 640, 748, 986, 1423, 1496, 1639
FaqI GGGAC 3 cut(s) 122, 548, 660
FatI CATG 9 cut(s) 103, 150, 520, 636, 744, 982, 1419, 1492, 1635
FauI CCCGC 2 cut(s) 1000, 1156
FbaI TGATCA 1 cut(s) 747
Fnu4HI GCNGC 5 cut(s) 252, 255, 275, 878, 1627
FokI GGATG 5 cut(s) 400, 490, 512, 809, 1088
FriOI GRGCYC 2 cut(s) 663, 902
Fsp4HI GCNGC 5 cut(s) 252, 255, 275, 878, 1627
FspBI CTAG 5 cut(s) 36, 86, 156, 728, 768
GluI GCNGC 5 cut(s) 252, 255, 275, 878, 1627
GsuI CTGGAG 2 cut(s) 380, 881
HaeIII GGCC 4 cut(s) 806, 900, 1256, 1282
HapII CCGG 2 cut(s) 218, 803
HgaI GACGC 2 cut(s) 203, 1349
Hin1I GRCGYC 2 cut(s) 214, 1341
Hin1II CATG 9 cut(s) 107, 154, 524, 640, 748, 986, 1423, 1496, 1639
HincII GTYRAC 2 cut(s) 307, 493
HindII GTYRAC 2 cut(s) 307, 493
HindIII AAGCTT 1 cut(s) 1213
HinfI GANTC 5 cut(s) 67, 167, 334, 1320, 1375
HpaII CCGG 2 cut(s) 218, 803
HphI GGTGA 7 cut(s) 76, 247, 553, 742, 1090, 1246, 1519
Hpy166II GTNNAC 3 cut(s) 174, 307, 493
Hpy188I TCNGA 2 cut(s) 778, 832
Hpy188III TCNNGA 9 cut(s) 218, 673, 860, 933, 1013, 1204, 1240, 1372, 1591
Hpy8I GTNNAC 3 cut(s) 174, 307, 493
Hpy99I CGWCG 1 cut(s) 463
HpyAV CCTTC 6 cut(s) 440, 498, 859, 1354, 1462, 1498
HpyCH4III ACNGT 2 cut(s) 651, 774
HpyCH4IV ACGT 3 cut(s) 135, 605, 907
HpyCH4V TGCA 6 cut(s) 254, 500, 615, 654, 1274, 1629
HpyF10VI GCNNNNNNNGC 8 cut(s) 220, 401, 521, 992, 1262, 1271, 1568, 1632
HpyF3I CTNAG 6 cut(s) 329, 530, 1216, 1241, 1352, 1615
HpySE526I ACGT 3 cut(s) 135, 605, 907
Hsp92I GRCGYC 2 cut(s) 214, 1341
Hsp92II CATG 9 cut(s) 107, 154, 524, 640, 748, 986, 1423, 1496, 1639
Kpn2I TCCGGA 1 cut(s) 217
Ksp22I TGATCA 1 cut(s) 747
Kzo9I GATC 4 cut(s) 699, 747, 1095, 1236
LmnI GCTCC 2 cut(s) 220, 282
Lsp1109I GCAGC 5 cut(s) 238, 266, 286, 864, 1613
LweI GCATC 6 cut(s) 391, 534, 1168, 1274, 1283, 1465
MaeI CTAG 5 cut(s) 36, 86, 156, 728, 768
MaeII ACGT 3 cut(s) 135, 605, 907
MaeIII GTNAC 6 cut(s) 158, 344, 597, 719, 948, 1017
MalI GATC 4 cut(s) 701, 749, 1097, 1238
MboI GATC 4 cut(s) 699, 747, 1095, 1236
MfeI CAATTG 1 cut(s) 311
MflI RGATCY 1 cut(s) 699
MhlI GDGCHC 3 cut(s) 663, 902, 1452
MmeI TCCRAC 3 cut(s) 37, 588, 1002
MroI TCCGGA 1 cut(s) 217
MroXI GAANNNNTTC 1 cut(s) 826
MseI TTAA 1 cut(s) 366
MslI CAYNNNNRTG 3 cut(s) 382, 641, 1232
MspI CCGG 2 cut(s) 218, 803
MspR9I CCNGG 2 cut(s) 296, 359
MunI CAATTG 1 cut(s) 311
Mva1269I GAATGC 1 cut(s) 617
MvaI CCWGG 2 cut(s) 296, 359
MwoI GCNNNNNNNGC 8 cut(s) 220, 401, 521, 992, 1262, 1271, 1568, 1632
NcoI CCATGG 1 cut(s) 1492
NdeII GATC 4 cut(s) 699, 747, 1095, 1236
NlaIII CATG 9 cut(s) 107, 154, 524, 640, 748, 986, 1423, 1496, 1639
NlaIV GGNNCC 3 cut(s) 900, 991, 1281
NmuCI GTSAC 2 cut(s) 158, 719
NspI RCATGY 1 cut(s) 1639
NspV TTCGAA 1 cut(s) 417
OliI CACNNNNGTG 1 cut(s) 1232
PaeR7I CTCGAG 1 cut(s) 1013
PctI GAATGC 1 cut(s) 617
PdmI GAANNNNTTC 1 cut(s) 826
PfeI GAWTC 5 cut(s) 67, 167, 334, 1320, 1375
PfoI TCCNGGA 1 cut(s) 294
PkrI GCNGC 5 cut(s) 253, 256, 276, 879, 1628
PpuMI RGGWCCY 1 cut(s) 298
Psp124BI GAGCTC 1 cut(s) 663
Psp1406I AACGTT 1 cut(s) 605
Psp5II RGGWCCY 1 cut(s) 298
Psp6I CCWGG 2 cut(s) 294, 357
PspGI CCWGG 2 cut(s) 294, 357
PspN4I GGNNCC 3 cut(s) 900, 991, 1281
PspOMI GGGCCC 1 cut(s) 898
PspPI GGNCC 8 cut(s) 197, 298, 800, 898, 899, 989, 1280, 1428
PspPPI RGGWCCY 1 cut(s) 298
PstI CTGCAG 2 cut(s) 256, 1631
PsuI RGATCY 1 cut(s) 699
RsaI GTAC 3 cut(s) 173, 356, 1210
RsaNI GTAC 3 cut(s) 172, 355, 1209
RseI CAYNNNNRTG 3 cut(s) 382, 641, 1232
SacI GAGCTC 1 cut(s) 663
SaqAI TTAA 1 cut(s) 366
SatI GCNGC 5 cut(s) 252, 255, 275, 878, 1627
Sau3AI GATC 4 cut(s) 699, 747, 1095, 1236
Sau96I GGNCC 8 cut(s) 197, 298, 800, 898, 899, 989, 1280, 1428
ScrFI CCNGG 2 cut(s) 296, 359
SduI GDGCHC 3 cut(s) 663, 902, 1452
SfaNI GCATC 6 cut(s) 391, 534, 1168, 1274, 1283, 1465
SfcI CTRYAG 3 cut(s) 252, 1621, 1627
Sfr274I CTCGAG 1 cut(s) 1013
SfuI TTCGAA 1 cut(s) 417
SinI GGWCC 5 cut(s) 197, 298, 800, 989, 1428
SlaI CTCGAG 1 cut(s) 1013
SmiMI CAYNNNNRTG 3 cut(s) 382, 641, 1232
SmlI CTYRAG 3 cut(s) 180, 548, 1013
SmoI CTYRAG 3 cut(s) 180, 548, 1013
SsiI CCGC 4 cut(s) 742, 993, 1079, 1149
SspMI CTAG 5 cut(s) 36, 86, 156, 728, 768
SstI GAGCTC 1 cut(s) 663
StyD4I CCNGG 2 cut(s) 294, 357
StyI CCWWGG 2 cut(s) 962, 1492
TaaI ACNGT 2 cut(s) 651, 774
TaiI ACGT 3 cut(s) 138, 608, 910
TatI WGTACW 1 cut(s) 1208
TfiI GAWTC 5 cut(s) 67, 167, 334, 1320, 1375
Tru1I TTAA 1 cut(s) 366
Tru9I TTAA 1 cut(s) 366
TseFI GTSAC 2 cut(s) 158, 719
TseI GCWGC 5 cut(s) 251, 254, 274, 877, 1626
Tsp45I GTSAC 2 cut(s) 158, 719
TspDTI ATGAA 5 cut(s) 692, 699, 1328, 1407, 1631
TspGWI ACGGA 1 cut(s) 1332
VpaK11BI GGWCC 5 cut(s) 197, 298, 800, 989, 1428
XapI RAATTY 4 cut(s) 80, 290, 841, 921
XceI RCATGY 1 cut(s) 1639
XcmI CCANNNNNNNNNTGG 1 cut(s) 148
XhoI CTCGAG 1 cut(s) 1013
XmnI GAANNNNTTC 1 cut(s) 826
XspI CTAG 5 cut(s) 36, 86, 156, 728, 768
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.