Rh5BG315900

UPF0481 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000833
Physical Location & Seq
Forward (+)
41978334 .. 41979563
1230 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG315900.1

Sequence Viewer

Length: 1230 bp
ATGGCCATTGATGCTTCGTTTTTCCTCGAAATCCTCCAAGTCTATGAGGTCCAAGAAGGCCCAAGAGTTTCTTCAAGGATGGCGCATTTGGTTGACCATGCAGGAAGGAAGTCAGCGCATCATGCAATCCTTAGGGATTTGGTGATGCTTGAGAACCAAATCCCACGATTTGTTTTGAAAAAAGTGTTGAAGTTTAAGCTCCCAGCGTTGGAATGTGCCAAAGATATGCTTCTTTCCATGTTTATGGGATTGTGCAATGAGCTCTCCCCATTCAAGATGAATGACGAAGACATGCCAAATATGGGAGTGACTATCCAATTCTCGAGTTGTTTTAACTTGCTGGACTCCTTGTACCGCCTGATCACCCCCAAGTTAGTAGCCGAGCCATCGGAAATCACAGAAGCCGTGGGATATGAGGCCGCACAACAACAGAAGGGAAAATCATCAGTTAATTCGAATTTCGTCAAACAATTTCTGAAGCAGGTACGGAAGCTGCTATCGAAGTTGAATAAAGGCCCAATACCTCTTCTGAGAAAAATTCCAATATATGGACCTGTAAAAGTGATATCCGACCTGCCTTGGACTATCCTCTTTGACCTTCTTTCCACCCAAGACAAAGAAGCAGTCAAACCCGAAGATGACAGTTCGAGCAATGGCATTAGTAAACCGCCGAAGATAGAGGAGATCACCATCCCATCAGTCTCCGATCTCTCAAAATCTGGTGTCAAGTTCTTGGCCGGTGGAATTAGAATTTCAGACATCTCTTTCAATGCTGAAACTGGCTCATTTACCCTCCCGATTACTAGCTTAGATGGGAACACAGGGGTGATCCTGAGAAACTTGGTGGCCTATGAAGCATCAAGTGTATCGGGGCCATTGGTTTTCACTCGCTACACCGGGTTGATGAACGGGATCATAGACACCGAGGAGGATGTCAAGTTGCTTAGAGAGAAGCGTATCATCATGAACCATTTGAAGAGTGATGAAGAAGTGGCAAATCTGCTCAATGGCATGAGCAGGTCCATTAGATTGACCAAAGTGCCCTCCTTGGATAAGGTAATCGCAGATGCCAATGAGTATTACAATGGTAGATGGAAAGTGAAGATGAGGAAGTTTTTGAACACTTTTGTGTATGGTTCATGGCCGTTACTGGCTTTCCTCGCTGCCATTTTGCTCCTGCTCTTGATGACATTGCAAGCAGTCTGCTCAGTCTATAGGTGCAGTACGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

409

Amino Acids

45.66

Weight (kDa)

8.88

Isoelectric Point (pI)

33.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 1 - 395 4.6e-68 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 472, 582, 1008
AccB7I CCANNNNNTGG 1 cut(s) 548
AciI CCGC 3 cut(s) 355, 420, 668
AclWI GGATC 2 cut(s) 823, 920
AcoI YGGCCR 3 cut(s) 3, 735, 1142
AcsI RAATTY 3 cut(s) 457, 537, 750
AcuI CTGAAG 1 cut(s) 497
AfaI GTAC 3 cut(s) 353, 486, 1225
AfiI CCNNNNNNNGG 3 cut(s) 208, 302, 548
AgsI TTSAA 8 cut(s) 75, 178, 190, 274, 508, 769, 976, 1120
AjuI GAANNNNNNNTTGG 2 cut(s) 55, 87
AleI CACNNNNGTG 2 cut(s) 824, 1127
AluBI AGCT 4 cut(s) 199, 262, 493, 807
AluI AGCT 4 cut(s) 199, 262, 493, 807
Alw21I GWGCWC 1 cut(s) 264
Alw26I GTCTC 1 cut(s) 706
AlwI GGATC 2 cut(s) 823, 920
Ama87I CYCGRG 1 cut(s) 322
AoxI GGCC 8 cut(s) 3, 58, 417, 514, 735, 846, 872, 1142
ApeKI GCWGC 2 cut(s) 493, 1163
ApoI RAATTY 3 cut(s) 457, 537, 750
ArsI GACNNNNNNTTYG 2 cut(s) 609, 641
AspLEI GCGC 2 cut(s) 85, 118
AspS9I GGNCC 6 cut(s) 49, 59, 515, 551, 872, 1020
AsuC2I CCSGG 1 cut(s) 898
AsuHPI GGTGA 4 cut(s) 154, 355, 679, 838
AsuII TTCGAA 1 cut(s) 455
AvaI CYCGRG 1 cut(s) 322
AvaII GGWCC 3 cut(s) 49, 551, 1020
AxyI CCTNAGG 1 cut(s) 131
BaeGI GKGCMC 1 cut(s) 1044
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 264
BbsI GAAGAC 1 cut(s) 294
Bbv12I GWGCWC 1 cut(s) 264
BbvI GCAGC 2 cut(s) 480, 1150
BccI CCATC 6 cut(s) 73, 394, 698, 703, 806, 1086
BceAI ACGGC 2 cut(s) 389, 1129
BclI TGATCA 1 cut(s) 360
BcnI CCSGG 1 cut(s) 898
BcoDI GTCTC 1 cut(s) 706
BfaI CTAG 1 cut(s) 804
BfmI CTRYAG 1 cut(s) 1213
BfuAI ACCTGC 3 cut(s) 472, 582, 1008
BisI GCNGC 3 cut(s) 420, 494, 1164
BlsI GCNGC 3 cut(s) 421, 495, 1165
Bme1390I CCNGG 1 cut(s) 898
Bme18I GGWCC 3 cut(s) 49, 551, 1020
BmeT110I CYCGRG 1 cut(s) 322
BmgT120I GGNCC 6 cut(s) 49, 59, 515, 551, 872, 1020
BmiI GGNNCC 1 cut(s) 873
BmrFI CCNGG 1 cut(s) 898
BmsI GCATC 4 cut(s) 127, 135, 866, 1057
BpiI GAAGAC 1 cut(s) 294
Bpu14I TTCGAA 1 cut(s) 455
BpuEI CTTGAG 1 cut(s) 170
BpuMI CCSGG 1 cut(s) 898
BsaAI YACGTR 1 cut(s) 1227
BsaBI GATNNNNATC 1 cut(s) 689
BsaJI CCNNGG 4 cut(s) 405, 578, 924, 1047
Bsc4I CCNNNNNNNGG 3 cut(s) 208, 302, 548
Bse118I RCCGGY 1 cut(s) 737
Bse1I ACTGG 2 cut(s) 784, 1155
Bse21I CCTNAGG 1 cut(s) 131
Bse3DI GCAATG 3 cut(s) 262, 658, 1190
Bse8I GATNNNNATC 1 cut(s) 689
BseDI CCNNGG 4 cut(s) 405, 578, 924, 1047
BseGI GGATG 3 cut(s) 84, 690, 937
BseJI GATNNNNATC 1 cut(s) 689
BseLI CCNNNNNNNGG 3 cut(s) 208, 302, 548
BseMI GCAATG 3 cut(s) 262, 658, 1190
BseMII CTCAG 3 cut(s) 521, 824, 1221
BseNI ACTGG 2 cut(s) 784, 1155
BseRI GAGGAG 2 cut(s) 695, 941
BseSI GKGCMC 1 cut(s) 1044
BseXI GCAGC 2 cut(s) 480, 1150
BseYI CCCAGC 1 cut(s) 202
BshFI GGCC 8 cut(s) 5, 60, 419, 516, 737, 848, 874, 1144
BsiHKAI GWGCWC 1 cut(s) 264
BsiHKCI CYCGRG 1 cut(s) 322
BsiSI CCGG 2 cut(s) 738, 897
BslI CCNNNNNNNGG 3 cut(s) 208, 302, 548
BsmAI GTCTC 1 cut(s) 706
BsnI GGCC 8 cut(s) 5, 60, 419, 516, 737, 848, 874, 1144
BsoBI CYCGRG 1 cut(s) 322
Bsp119I TTCGAA 1 cut(s) 455
Bsp1286I GDGCHC 2 cut(s) 264, 1044
Bsp143I GATC 5 cut(s) 360, 684, 706, 828, 912
BspACI CCGC 3 cut(s) 355, 420, 668
BspANI GGCC 8 cut(s) 5, 60, 419, 516, 737, 848, 874, 1144
BspCNI CTCAG 3 cut(s) 522, 825, 1220
BspHI TCATGA 1 cut(s) 963
BspLI GGNNCC 1 cut(s) 873
BspMI ACCTGC 3 cut(s) 472, 582, 1008
BspPI GGATC 2 cut(s) 823, 920
BspT104I TTCGAA 1 cut(s) 455
BsrDI GCAATG 3 cut(s) 262, 658, 1190
BsrFI RCCGGY 1 cut(s) 737
BsrI ACTGG 2 cut(s) 784, 1155
BssAI RCCGGY 1 cut(s) 737
BssECI CCNNGG 4 cut(s) 405, 578, 924, 1047
BssMI GATC 5 cut(s) 360, 684, 706, 828, 912
BssT1I CCWWGG 2 cut(s) 578, 1047
Bst4CI ACNGT 1 cut(s) 644
Bst6I CTCTTC 2 cut(s) 531, 971
BstBAI YACGTR 1 cut(s) 1227
BstBI TTCGAA 1 cut(s) 455
BstC8I GCNNGC 1 cut(s) 1197
BstDEI CTNAG 6 cut(s) 131, 530, 808, 833, 944, 1207
BstDSI CCRYGG 1 cut(s) 405
BstF5I GGATG 3 cut(s) 84, 690, 937
BstHHI GCGC 2 cut(s) 85, 118
BstKTI GATC 5 cut(s) 363, 687, 709, 831, 915
BstMAI GTCTC 1 cut(s) 706
BstMBI GATC 5 cut(s) 360, 684, 706, 828, 912
BstMWI GCNNNNNNNGC 4 cut(s) 11, 122, 854, 1160
BstNSI RCATGY 1 cut(s) 295
BstSCI CCNGG 1 cut(s) 896
BstSFI CTRYAG 1 cut(s) 1213
BstSLI GKGCMC 1 cut(s) 1044
BstSNI TACGTA 1 cut(s) 1227
BstV1I GCAGC 2 cut(s) 480, 1150
BstV2I GAAGAC 1 cut(s) 294
BstXI CCANNNNNNTGG 1 cut(s) 244
Bsu36I CCTNAGG 1 cut(s) 131
BsuRI GGCC 8 cut(s) 5, 60, 419, 516, 737, 848, 874, 1144
BtgI CCRYGG 1 cut(s) 405
BtsCI GGATG 3 cut(s) 84, 690, 937
BveI ACCTGC 3 cut(s) 472, 582, 1008
Cac8I GCNNGC 1 cut(s) 1197
CciI TCATGA 1 cut(s) 963
CfoI GCGC 2 cut(s) 85, 118
Cfr10I RCCGGY 1 cut(s) 737
Cfr13I GGNCC 6 cut(s) 49, 59, 515, 551, 872, 1020
Csp6I GTAC 3 cut(s) 352, 485, 1224
CviAII CATG 7 cut(s) 98, 122, 238, 292, 964, 1012, 1140
CviQI GTAC 3 cut(s) 352, 485, 1224
DdeI CTNAG 6 cut(s) 131, 530, 808, 833, 944, 1207
DpnI GATC 5 cut(s) 362, 686, 708, 830, 914
DpnII GATC 5 cut(s) 360, 684, 706, 828, 912
EaeI YGGCCR 3 cut(s) 3, 735, 1142
Eam1104I CTCTTC 2 cut(s) 531, 971
EarI CTCTTC 2 cut(s) 531, 971
Ecl136II GAGCTC 1 cut(s) 262
Eco105I TACGTA 1 cut(s) 1227
Eco130I CCWWGG 2 cut(s) 578, 1047
Eco24I GRGCYC 1 cut(s) 264
Eco32I GATATC 1 cut(s) 567
Eco47I GGWCC 3 cut(s) 49, 551, 1020
Eco53kI GAGCTC 1 cut(s) 262
Eco57I CTGAAG 1 cut(s) 497
Eco81I CCTNAGG 1 cut(s) 131
Eco88I CYCGRG 1 cut(s) 322
EcoICRI GAGCTC 1 cut(s) 262
EcoRV GATATC 1 cut(s) 567
EcoT14I CCWWGG 2 cut(s) 578, 1047
EcoT38I GRGCYC 1 cut(s) 264
ErhI CCWWGG 2 cut(s) 578, 1047
FaeI CATG 7 cut(s) 101, 125, 241, 295, 967, 1015, 1143
FalI AAGNNNNNCTT 4 cut(s) 55, 87, 213, 245
FatI CATG 7 cut(s) 97, 121, 237, 291, 963, 1011, 1139
FbaI TGATCA 1 cut(s) 360
Fnu4HI GCNGC 3 cut(s) 420, 494, 1164
FokI GGATG 3 cut(s) 91, 677, 944
FriOI GRGCYC 1 cut(s) 264
Fsp4HI GCNGC 3 cut(s) 420, 494, 1164
FspBI CTAG 1 cut(s) 804
GlaI GCGC 2 cut(s) 84, 117
GluI GCNGC 3 cut(s) 420, 494, 1164
GsaI CCCAGC 1 cut(s) 206
HaeIII GGCC 8 cut(s) 5, 60, 419, 516, 737, 848, 874, 1144
HapII CCGG 2 cut(s) 738, 897
HhaI GCGC 2 cut(s) 85, 118
Hin1II CATG 7 cut(s) 101, 125, 241, 295, 967, 1015, 1143
Hin6I GCGC 2 cut(s) 83, 116
HinP1I GCGC 2 cut(s) 83, 116
HincII GTYRAC 1 cut(s) 94
HindII GTYRAC 1 cut(s) 94
HinfI GANTC 1 cut(s) 344
HpaII CCGG 2 cut(s) 738, 897
HphI GGTGA 4 cut(s) 154, 355, 679, 838
Hpy166II GTNNAC 2 cut(s) 94, 665
Hpy188I TCNGA 6 cut(s) 391, 477, 531, 571, 706, 757
Hpy188III TCNNGA 6 cut(s) 274, 322, 796, 832, 964, 1183
Hpy8I GTNNAC 2 cut(s) 94, 665
HpyAV CCTTC 4 cut(s) 50, 99, 427, 608
HpyCH4III ACNGT 1 cut(s) 644
HpyCH4IV ACGT 1 cut(s) 1226
HpyCH4V TGCA 5 cut(s) 101, 125, 255, 1195, 1221
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 122, 854, 1160
HpyF3I CTNAG 6 cut(s) 131, 530, 808, 833, 944, 1207
HpySE526I ACGT 1 cut(s) 1226
Hsp92II CATG 7 cut(s) 101, 125, 241, 295, 967, 1015, 1143
HspAI GCGC 2 cut(s) 83, 116
Ksp22I TGATCA 1 cut(s) 360
Kzo9I GATC 5 cut(s) 360, 684, 706, 828, 912
LmnI GCTCC 2 cut(s) 204, 1179
Lsp1109I GCAGC 2 cut(s) 480, 1150
LweI GCATC 4 cut(s) 127, 135, 866, 1057
MaeI CTAG 1 cut(s) 804
MaeII ACGT 1 cut(s) 1226
MaeIII GTNAC 2 cut(s) 307, 1146
MalI GATC 5 cut(s) 362, 686, 708, 830, 914
MboI GATC 5 cut(s) 360, 684, 706, 828, 912
MboII GAAGA 8 cut(s) 63, 299, 518, 647, 685, 988, 998, 1114
MhlI GDGCHC 2 cut(s) 264, 1044
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 7 cut(s) 317, 451, 457, 470, 537, 744, 750
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 338
MmeI TCCRAC 2 cut(s) 189, 594
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 195, 333, 450
MslI CAYNNNNRTG 3 cut(s) 242, 824, 1127
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 2 cut(s) 738, 897
MspR9I CCNGG 1 cut(s) 898
MwoI GCNNNNNNNGC 4 cut(s) 11, 122, 854, 1160
NciI CCSGG 1 cut(s) 898
NdeII GATC 5 cut(s) 360, 684, 706, 828, 912
NlaIII CATG 7 cut(s) 101, 125, 241, 295, 967, 1015, 1143
NlaIV GGNNCC 1 cut(s) 873
NmeAIII GCCGAG 1 cut(s) 406
NmuCI GTSAC 1 cut(s) 307
NspI RCATGY 1 cut(s) 295
NspV TTCGAA 1 cut(s) 455
OliI CACNNNNGTG 2 cut(s) 824, 1127
PaeR7I CTCGAG 1 cut(s) 322
PagI TCATGA 1 cut(s) 963
PflMI CCANNNNNTGG 1 cut(s) 548
PkrI GCNGC 3 cut(s) 421, 495, 1165
PleI GAGTC 1 cut(s) 338
PpsI GAGTC 1 cut(s) 338
Ppu21I YACGTR 1 cut(s) 1227
Psp124BI GAGCTC 1 cut(s) 264
PspFI CCCAGC 1 cut(s) 202
PspN4I GGNNCC 1 cut(s) 873
PspPI GGNCC 6 cut(s) 49, 59, 515, 551, 872, 1020
RsaI GTAC 3 cut(s) 353, 486, 1225
RsaNI GTAC 3 cut(s) 352, 485, 1224
RseI CAYNNNNRTG 3 cut(s) 242, 824, 1127
SacI GAGCTC 1 cut(s) 264
SaqAI TTAA 3 cut(s) 195, 333, 450
SatI GCNGC 3 cut(s) 420, 494, 1164
Sau3AI GATC 5 cut(s) 360, 684, 706, 828, 912
Sau96I GGNCC 6 cut(s) 49, 59, 515, 551, 872, 1020
SchI GAGTC 1 cut(s) 338
ScrFI CCNGG 1 cut(s) 898
SduI GDGCHC 2 cut(s) 264, 1044
SfaNI GCATC 4 cut(s) 127, 135, 866, 1057
SfcI CTRYAG 1 cut(s) 1213
Sfr274I CTCGAG 1 cut(s) 322
SfuI TTCGAA 1 cut(s) 455
SinI GGWCC 3 cut(s) 49, 551, 1020
SlaI CTCGAG 1 cut(s) 322
SmiMI CAYNNNNRTG 3 cut(s) 242, 824, 1127
SmlI CTYRAG 2 cut(s) 149, 322
SmoI CTYRAG 2 cut(s) 149, 322
SnaBI TACGTA 1 cut(s) 1227
Sse9I AATT 7 cut(s) 317, 451, 457, 470, 537, 744, 750
SsiI CCGC 3 cut(s) 355, 420, 668
SspMI CTAG 1 cut(s) 804
SstI GAGCTC 1 cut(s) 264
StyD4I CCNGG 1 cut(s) 896
StyI CCWWGG 2 cut(s) 578, 1047
TaaI ACNGT 1 cut(s) 644
TaiI ACGT 1 cut(s) 1229
TaqI TCGA 5 cut(s) 27, 323, 455, 500, 647
TasI AATT 7 cut(s) 317, 451, 457, 470, 537, 744, 750
TauI GCSGC 1 cut(s) 422
Tru1I TTAA 3 cut(s) 195, 333, 450
Tru9I TTAA 3 cut(s) 195, 333, 450
TseFI GTSAC 1 cut(s) 307
TseI GCWGC 2 cut(s) 493, 1163
Tsp45I GTSAC 1 cut(s) 307
TspDTI ATGAA 6 cut(s) 293, 867, 920, 980, 999, 1128
TspGWI ACGGA 1 cut(s) 502
Van91I CCANNNNNTGG 1 cut(s) 548
VpaK11BI GGWCC 3 cut(s) 49, 551, 1020
XapI RAATTY 3 cut(s) 457, 537, 750
XceI RCATGY 1 cut(s) 295
XhoI CTCGAG 1 cut(s) 322
XspI CTAG 1 cut(s) 804
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.