Rmu_ssc0000213.1_g000105

UPF0481 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000213.1
OG0000833
Physical Location & Seq
Forward (+)
403063 .. 404727
1665 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000213.1_g000105.1.cds

Sequence Viewer

Length: 1665 bp
atgtcttctctccaaccaacattctcttccaattccagttcaaacttcaatgagcttcaatgggtgattcaaatccaaaaattcctagaggaagagttcgagcatgatatcgaaatccctatctccattttcaacgtccccaaatccctcatggctagtgacccagaatcgtacacccctcaagtggttgcgattggtccttaccatcattggcgtccggagctgtatgagatggtgaggtacaaagtggctgcagctaaaagaacccaaaagcagctccaaaccctcaaattccaggaccttgttgaccaattgacaaggttcgagctgaggattcgggcttgttaccacaagtacctggagtttaatggcgagacattggggtggatgatggctattgatgcttcgtttttcttcgaaatcctccaagtctatgcagtccaagaagggaacacttcgacgagagtttcttcaaggatggcgcatttggttgactatgcaggaaggaagtcagcgcatcatgccatccttagggacttggtgatgcttgagaaccaaatgccactatttgttttaagagaagtgttggcatttcagttaccaacgttggaatgcgccgacaatatgcttttttccatgtttatgggactgtgcaaagagctctccccattcaagatgaatgatgaagctatgccaaagttccaagtgtcgagttgttgtcacttgctagactttttataccgcatgatcacccccaaattagaaactagaccgtccgaaattgcagaaaccgaggatggtacggccacaccacacgagggaaagtcttcagataactcgaatttcctcaaacaatttctggagaaggtagggaagctgctattgaagttgaacaagggcccgatacgtcttttgaaaaaaattttaaattctcgacctgtaaaagtgttacgcaaactgccttggaccttcctttccaaccatgctagacccgcaatttggaaacaacctctcgagttactttcctcccaagacaaagaagcagtcaaacccggagatgataaatcaagcaatagaccaccgttgatagaggagatcaccatcccatcagtctccaaactctcaaaatcaggtgtcaagttcttgcccgcaagtagtatttcaaccatctctttcgatgctaaaacttgctcattttacctcccgattacaagcttagatgggaacacagaggtgatcatgagaaacttggtggcctacgaggcatcaactgcatcggggccattggtttttactcgctacaccgagttgatgaacggaatcatagacaccaaggaggacgccaagttgcttagagagaagggtatcatcttgaaccgtttgaagagtgatgaagaagtggcaaatctgtttaatggcatgagcaggtccattagattgaccaaagtgcccttcttggataaggcaatcaaagatgccaacaagtattaccatggtagatggcaggtgaagatgagggagtcttcgaaaacttatgtgttaggttcttggcaatttctggcttcgctcgctaccatttttctcctgcaattgatgacattggaagcattttgctcagtctatagctgcagtcgcatgtttcatatcaacaccactaataaatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

554

Amino Acids

63.39

Weight (kDa)

8.84

Isoelectric Point (pI)

45.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1495
Acc36I ACCTGC 2 cut(s) 1416, 1495
AccIII TCCGGA 1 cut(s) 217
AciI CCGC 3 cut(s) 742, 993, 1149
AclI AACGTT 1 cut(s) 605
AcoI YGGCCR 1 cut(s) 804
AcsI RAATTY 5 cut(s) 80, 290, 841, 921, 928
AcuI CTGAAG 1 cut(s) 813
AcyI GRCGYC 2 cut(s) 214, 1341
AfaI GTAC 4 cut(s) 173, 242, 356, 802
AfiI CCNNNNNNNGG 4 cut(s) 184, 531, 818, 999
AjnI CCWGG 2 cut(s) 294, 357
AjuI GAANNNNNNNTTGG 4 cut(s) 10, 42, 1436, 1468
AleI CACNNNNGTG 1 cut(s) 1232
Alw21I GWGCWC 1 cut(s) 663
Alw26I GTCTC 2 cut(s) 368, 1117
Ama87I CYCGRG 1 cut(s) 1013
Aor13HI TCCGGA 1 cut(s) 217
AoxI GGCC 4 cut(s) 804, 898, 1254, 1280
ApaI GGGCCC 1 cut(s) 902
ApeKI GCWGC 5 cut(s) 251, 254, 274, 877, 1626
ApoI RAATTY 5 cut(s) 80, 290, 841, 921, 928
ArsI GACNNNNNNTTYG 4 cut(s) 981, 1013, 1029, 1061
Asp700I GAANNNNTTC 1 cut(s) 826
AspLEI GCGC 3 cut(s) 484, 517, 617
AspS9I GGNCC 7 cut(s) 197, 298, 898, 899, 966, 1280, 1428
AsuC2I CCSGG 1 cut(s) 1053
AsuHPI GGTGA 7 cut(s) 76, 247, 553, 742, 1090, 1246, 1519
AsuII TTCGAA 2 cut(s) 417, 1526
AvaI CYCGRG 1 cut(s) 1013
AvaII GGWCC 4 cut(s) 197, 298, 966, 1428
AxyI CCTNAGG 1 cut(s) 530
BaeGI GKGCMC 2 cut(s) 902, 1452
BanII GRGCYC 2 cut(s) 663, 902
BauI CACGAG 1 cut(s) 815
BbsI GAAGAC 2 cut(s) 819, 1515
Bbv12I GWGCWC 1 cut(s) 663
BbvCI CCTCAGC 1 cut(s) 329
BbvI GCAGC 5 cut(s) 238, 266, 286, 864, 1613
BceAI ACGGC 1 cut(s) 819
BciT130I CCWGG 2 cut(s) 296, 359
BclI TGATCA 2 cut(s) 747, 1236
BcnI CCSGG 1 cut(s) 1053
BcoDI GTCTC 2 cut(s) 368, 1117
BfaI CTAG 5 cut(s) 86, 156, 728, 768, 987
BfmI CTRYAG 3 cut(s) 252, 1621, 1627
BfuAI ACCTGC 2 cut(s) 1416, 1495
BglI GCCNNNNNGGC 1 cut(s) 1262
BisI GCNGC 5 cut(s) 252, 255, 275, 878, 1627
BlsI GCNGC 5 cut(s) 253, 256, 276, 879, 1628
Bme1390I CCNGG 3 cut(s) 296, 359, 1053
Bme18I GGWCC 4 cut(s) 197, 298, 966, 1428
BmeT110I CYCGRG 1 cut(s) 1013
BmgT120I GGNCC 7 cut(s) 197, 298, 898, 899, 966, 1280, 1428
BmiI GGNNCC 2 cut(s) 900, 1281
BmrFI CCNGG 3 cut(s) 296, 359, 1053
BmsI GCATC 7 cut(s) 391, 526, 534, 1168, 1274, 1283, 1465
BpiI GAAGAC 2 cut(s) 819, 1515
BpmI CTGGAG 2 cut(s) 380, 881
Bpu10I CCTNAGC 1 cut(s) 329
Bpu14I TTCGAA 2 cut(s) 417, 1526
BpuEI CTTGAG 2 cut(s) 165, 569
BpuMI CCSGG 1 cut(s) 1053
BsaBI GATNNNNATC 2 cut(s) 71, 1100
BsaHI GRCGYC 2 cut(s) 214, 1341
BsaJI CCNNGG 4 cut(s) 792, 962, 1332, 1492
BsaWI WCCGGW 1 cut(s) 217
Bsc4I CCNNNNNNNGG 4 cut(s) 184, 531, 818, 999
Bse1I ACTGG 1 cut(s) 36
Bse21I CCTNAGG 1 cut(s) 530
Bse8I GATNNNNATC 2 cut(s) 71, 1100
BseAI TCCGGA 1 cut(s) 217
BseBI CCWGG 2 cut(s) 296, 359
BseDI CCNNGG 4 cut(s) 792, 962, 1332, 1492
BseGI GGATG 5 cut(s) 393, 483, 525, 802, 1101
BseJI GATNNNNATC 2 cut(s) 71, 1100
BseLI CCNNNNNNNGG 4 cut(s) 184, 531, 818, 999
BseMII CTCAG 2 cut(s) 320, 1629
BseNI ACTGG 1 cut(s) 36
BseRI GAGGAG 1 cut(s) 1106
BseSI GKGCMC 2 cut(s) 902, 1452
BseXI GCAGC 5 cut(s) 238, 266, 286, 864, 1613
BshFI GGCC 4 cut(s) 806, 900, 1256, 1282
BsiHKAI GWGCWC 1 cut(s) 663
BsiHKCI CYCGRG 1 cut(s) 1013
BsiSI CCGG 2 cut(s) 218, 1053
BslFI GGGAC 3 cut(s) 122, 548, 660
BslI CCNNNNNNNGG 4 cut(s) 184, 531, 818, 999
BsmAI GTCTC 2 cut(s) 368, 1117
BsmFI GGGAC 3 cut(s) 122, 548, 660
BsmI GAATGC 1 cut(s) 617
BsnI GGCC 4 cut(s) 806, 900, 1256, 1282
BsoBI CYCGRG 1 cut(s) 1013
Bsp119I TTCGAA 2 cut(s) 417, 1526
Bsp120I GGGCCC 1 cut(s) 898
Bsp1286I GDGCHC 3 cut(s) 663, 902, 1452
Bsp13I TCCGGA 1 cut(s) 217
Bsp143I GATC 3 cut(s) 747, 1095, 1236
Bsp19I CCATGG 1 cut(s) 1492
BspACI CCGC 3 cut(s) 742, 993, 1149
BspANI GGCC 4 cut(s) 806, 900, 1256, 1282
BspCNI CTCAG 2 cut(s) 321, 1628
BspEI TCCGGA 1 cut(s) 217
BspHI TCATGA 1 cut(s) 1239
BspLI GGNNCC 2 cut(s) 900, 1281
BspMAI CTGCAG 2 cut(s) 256, 1631
BspMI ACCTGC 2 cut(s) 1416, 1495
BspT104I TTCGAA 2 cut(s) 417, 1526
BsrI ACTGG 1 cut(s) 36
BssECI CCNNGG 4 cut(s) 792, 962, 1332, 1492
BssMI GATC 3 cut(s) 747, 1095, 1236
BssNI GRCGYC 2 cut(s) 214, 1341
BssSI CACGAG 1 cut(s) 815
BssT1I CCWWGG 3 cut(s) 962, 1332, 1492
Bst2BI CACGAG 1 cut(s) 815
Bst2UI CCWGG 2 cut(s) 296, 359
Bst4CI ACNGT 4 cut(s) 651, 774, 1083, 1379
Bst6I CTCTTC 3 cut(s) 31, 87, 1379
BstACI GRCGYC 2 cut(s) 214, 1341
BstAPI GCANNNNNTGC 1 cut(s) 1271
BstBI TTCGAA 2 cut(s) 417, 1526
BstC8I GCNNGC 2 cut(s) 1149, 1569
BstDEI CTNAG 5 cut(s) 329, 530, 1216, 1352, 1615
BstDSI CCRYGG 1 cut(s) 1492
BstF5I GGATG 5 cut(s) 393, 483, 525, 802, 1101
BstHHI GCGC 3 cut(s) 484, 517, 617
BstKTI GATC 3 cut(s) 750, 1098, 1239
BstMAI GTCTC 2 cut(s) 368, 1117
BstMBI GATC 3 cut(s) 747, 1095, 1236
BstMWI GCNNNNNNNGC 8 cut(s) 220, 401, 521, 992, 1262, 1271, 1568, 1632
BstNI CCWGG 2 cut(s) 296, 359
BstNSI RCATGY 1 cut(s) 1639
BstSCI CCNGG 3 cut(s) 294, 357, 1051
BstSFI CTRYAG 3 cut(s) 252, 1621, 1627
BstSLI GKGCMC 2 cut(s) 902, 1452
BstV1I GCAGC 5 cut(s) 238, 266, 286, 864, 1613
BstV2I GAAGAC 2 cut(s) 819, 1515
BstXI CCANNNNNNTGG 1 cut(s) 643
Bsu36I CCTNAGG 1 cut(s) 530
BsuRI GGCC 4 cut(s) 806, 900, 1256, 1282
BtgI CCRYGG 1 cut(s) 1492
BtsCI GGATG 5 cut(s) 393, 483, 525, 802, 1101
BveI ACCTGC 2 cut(s) 1416, 1495
Cac8I GCNNGC 2 cut(s) 1149, 1569
CciI TCATGA 1 cut(s) 1239
CfoI GCGC 3 cut(s) 484, 517, 617
Cfr13I GGNCC 7 cut(s) 197, 298, 898, 899, 966, 1280, 1428
CseI GACGC 2 cut(s) 203, 1349
Csp6I GTAC 4 cut(s) 172, 241, 355, 801
CviQI GTAC 4 cut(s) 172, 241, 355, 801
DdeI CTNAG 5 cut(s) 329, 530, 1216, 1352, 1615
DpnI GATC 3 cut(s) 749, 1097, 1238
DpnII GATC 3 cut(s) 747, 1095, 1236
DraI TTTAAA 1 cut(s) 927
EaeI YGGCCR 1 cut(s) 804
Eam1104I CTCTTC 3 cut(s) 31, 87, 1379
EarI CTCTTC 3 cut(s) 31, 87, 1379
Ecl136II GAGCTC 1 cut(s) 661
Eco130I CCWWGG 3 cut(s) 962, 1332, 1492
Eco24I GRGCYC 2 cut(s) 663, 902
Eco32I GATATC 1 cut(s) 109
Eco47I GGWCC 4 cut(s) 197, 298, 966, 1428
Eco53kI GAGCTC 1 cut(s) 661
Eco57I CTGAAG 1 cut(s) 813
Eco81I CCTNAGG 1 cut(s) 530
Eco88I CYCGRG 1 cut(s) 1013
EcoICRI GAGCTC 1 cut(s) 661
EcoO109I RGGNCCY 2 cut(s) 298, 898
EcoRII CCWGG 2 cut(s) 294, 357
EcoRV GATATC 1 cut(s) 109
EcoT14I CCWWGG 3 cut(s) 962, 1332, 1492
EcoT38I GRGCYC 2 cut(s) 663, 902
ErhI CCWWGG 3 cut(s) 962, 1332, 1492
FaqI GGGAC 3 cut(s) 122, 548, 660
FauI CCCGC 2 cut(s) 1000, 1156
FbaI TGATCA 2 cut(s) 747, 1236
Fnu4HI GCNGC 5 cut(s) 252, 255, 275, 878, 1627
FokI GGATG 5 cut(s) 400, 490, 512, 809, 1088
FriOI GRGCYC 2 cut(s) 663, 902
Fsp4HI GCNGC 5 cut(s) 252, 255, 275, 878, 1627
FspBI CTAG 5 cut(s) 86, 156, 728, 768, 987
GlaI GCGC 3 cut(s) 483, 516, 616
GluI GCNGC 5 cut(s) 252, 255, 275, 878, 1627
GsuI CTGGAG 2 cut(s) 380, 881
HaeIII GGCC 4 cut(s) 806, 900, 1256, 1282
HapII CCGG 2 cut(s) 218, 1053
HgaI GACGC 2 cut(s) 203, 1349
HhaI GCGC 3 cut(s) 484, 517, 617
Hin1I GRCGYC 2 cut(s) 214, 1341
Hin6I GCGC 3 cut(s) 482, 515, 615
HinP1I GCGC 3 cut(s) 482, 515, 615
HincII GTYRAC 2 cut(s) 307, 493
HindII GTYRAC 2 cut(s) 307, 493
HindIII AAGCTT 1 cut(s) 1213
HinfI GANTC 5 cut(s) 67, 167, 334, 1320, 1520
HpaII CCGG 2 cut(s) 218, 1053
HphI GGTGA 7 cut(s) 76, 247, 553, 742, 1090, 1246, 1519
Hpy166II GTNNAC 3 cut(s) 174, 307, 493
Hpy188I TCNGA 2 cut(s) 778, 832
Hpy188III TCNNGA 8 cut(s) 218, 673, 860, 933, 1013, 1204, 1240, 1372
Hpy8I GTNNAC 3 cut(s) 174, 307, 493
Hpy99I CGWCG 1 cut(s) 463
HpyAV CCTTC 6 cut(s) 440, 498, 859, 979, 1354, 1462
HpyCH4III ACNGT 4 cut(s) 651, 774, 1083, 1379
HpyCH4IV ACGT 3 cut(s) 135, 605, 907
HpyCH4V TGCA 8 cut(s) 254, 437, 500, 654, 785, 1274, 1588, 1629
HpyF10VI GCNNNNNNNGC 8 cut(s) 220, 401, 521, 992, 1262, 1271, 1568, 1632
HpyF3I CTNAG 5 cut(s) 329, 530, 1216, 1352, 1615
HpySE526I ACGT 3 cut(s) 135, 605, 907
Hsp92I GRCGYC 2 cut(s) 214, 1341
HspAI GCGC 3 cut(s) 482, 515, 615
Kpn2I TCCGGA 1 cut(s) 217
Ksp22I TGATCA 2 cut(s) 747, 1236
Kzo9I GATC 3 cut(s) 747, 1095, 1236
LmnI GCTCC 2 cut(s) 220, 282
Lsp1109I GCAGC 5 cut(s) 238, 266, 286, 864, 1613
LweI GCATC 7 cut(s) 391, 526, 534, 1168, 1274, 1283, 1465
MaeI CTAG 5 cut(s) 86, 156, 728, 768, 987
MaeII ACGT 3 cut(s) 135, 605, 907
MaeIII GTNAC 6 cut(s) 158, 344, 597, 719, 948, 1017
MalI GATC 3 cut(s) 749, 1097, 1238
MboI GATC 3 cut(s) 747, 1095, 1236
MboII GAAGA 9 cut(s) 18, 104, 406, 462, 819, 1396, 1406, 1515, 1522
MfeI CAATTG 2 cut(s) 311, 1589
MhlI GDGCHC 3 cut(s) 663, 902, 1452
MlyI GAGTC 1 cut(s) 1529
MmeI TCCRAC 3 cut(s) 37, 588, 1002
MroI TCCGGA 1 cut(s) 217
MroXI GAANNNNTTC 1 cut(s) 826
MseI TTAA 4 cut(s) 366, 575, 926, 1413
MslI CAYNNNNRTG 3 cut(s) 382, 641, 1232
MspI CCGG 2 cut(s) 218, 1053
MspR9I CCNGG 3 cut(s) 296, 359, 1053
MunI CAATTG 2 cut(s) 311, 1589
Mva1269I GAATGC 1 cut(s) 617
MvaI CCWGG 2 cut(s) 296, 359
MwoI GCNNNNNNNGC 8 cut(s) 220, 401, 521, 992, 1262, 1271, 1568, 1632
NciI CCSGG 1 cut(s) 1053
NcoI CCATGG 1 cut(s) 1492
NdeII GATC 3 cut(s) 747, 1095, 1236
NlaIV GGNNCC 2 cut(s) 900, 1281
NmuCI GTSAC 2 cut(s) 158, 719
NspI RCATGY 1 cut(s) 1639
NspV TTCGAA 2 cut(s) 417, 1526
OliI CACNNNNGTG 1 cut(s) 1232
PaeR7I CTCGAG 1 cut(s) 1013
PagI TCATGA 1 cut(s) 1239
PaqCI CACCTGC 1 cut(s) 1495
PctI GAATGC 1 cut(s) 617
PdmI GAANNNNTTC 1 cut(s) 826
PfeI GAWTC 4 cut(s) 67, 167, 334, 1320
PfoI TCCNGGA 1 cut(s) 294
PkrI GCNGC 5 cut(s) 253, 256, 276, 879, 1628
PleI GAGTC 1 cut(s) 1528
PpsI GAGTC 1 cut(s) 1528
PpuMI RGGWCCY 1 cut(s) 298
Psp124BI GAGCTC 1 cut(s) 663
Psp1406I AACGTT 1 cut(s) 605
Psp5II RGGWCCY 1 cut(s) 298
Psp6I CCWGG 2 cut(s) 294, 357
PspGI CCWGG 2 cut(s) 294, 357
PspN4I GGNNCC 2 cut(s) 900, 1281
PspOMI GGGCCC 1 cut(s) 898
PspPI GGNCC 7 cut(s) 197, 298, 898, 899, 966, 1280, 1428
PspPPI RGGWCCY 1 cut(s) 298
PstI CTGCAG 2 cut(s) 256, 1631
RsaI GTAC 4 cut(s) 173, 242, 356, 802
RsaNI GTAC 4 cut(s) 172, 241, 355, 801
RseI CAYNNNNRTG 3 cut(s) 382, 641, 1232
SacI GAGCTC 1 cut(s) 663
SaqAI TTAA 4 cut(s) 366, 575, 926, 1413
SatI GCNGC 5 cut(s) 252, 255, 275, 878, 1627
Sau3AI GATC 3 cut(s) 747, 1095, 1236
Sau96I GGNCC 7 cut(s) 197, 298, 898, 899, 966, 1280, 1428
SchI GAGTC 1 cut(s) 1529
ScrFI CCNGG 3 cut(s) 296, 359, 1053
SduI GDGCHC 3 cut(s) 663, 902, 1452
SfaNI GCATC 7 cut(s) 391, 526, 534, 1168, 1274, 1283, 1465
SfcI CTRYAG 3 cut(s) 252, 1621, 1627
Sfr274I CTCGAG 1 cut(s) 1013
SfuI TTCGAA 2 cut(s) 417, 1526
SinI GGWCC 4 cut(s) 197, 298, 966, 1428
SlaI CTCGAG 1 cut(s) 1013
SmiMI CAYNNNNRTG 3 cut(s) 382, 641, 1232
SmlI CTYRAG 3 cut(s) 180, 548, 1013
SmoI CTYRAG 3 cut(s) 180, 548, 1013
SsiI CCGC 3 cut(s) 742, 993, 1149
SspMI CTAG 5 cut(s) 86, 156, 728, 768, 987
SstI GAGCTC 1 cut(s) 663
StyD4I CCNGG 3 cut(s) 294, 357, 1051
StyI CCWWGG 3 cut(s) 962, 1332, 1492
TaaI ACNGT 4 cut(s) 651, 774, 1083, 1379
TaiI ACGT 3 cut(s) 138, 608, 910
TfiI GAWTC 4 cut(s) 67, 167, 334, 1320
Tru1I TTAA 4 cut(s) 366, 575, 926, 1413
Tru9I TTAA 4 cut(s) 366, 575, 926, 1413
TseFI GTSAC 2 cut(s) 158, 719
TseI GCWGC 5 cut(s) 251, 254, 274, 877, 1626
Tsp45I GTSAC 2 cut(s) 158, 719
TspDTI ATGAA 5 cut(s) 692, 699, 1328, 1407, 1631
TspGWI ACGGA 1 cut(s) 1332
VpaK11BI GGWCC 4 cut(s) 197, 298, 966, 1428
XapI RAATTY 5 cut(s) 80, 290, 841, 921, 928
XceI RCATGY 1 cut(s) 1639
XcmI CCANNNNNNNNNTGG 1 cut(s) 148
XhoI CTCGAG 1 cut(s) 1013
XmnI GAANNNNTTC 1 cut(s) 826
XspI CTAG 5 cut(s) 86, 156, 728, 768, 987
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.