AT5G21030

Belongs to the argonaute family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0001623
Physical Location & Seq
Reverse (-)
7139817 .. 7144272
4456 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G21030.1

Sequence Viewer

Length: 2553 bp
ATGGATACGACTCTACCGCCTCCTCAACACATGGAACGTGAACCTTTGAAGAGCAAGAGTAGTCTTCTTCCAATGACTCGCCGTGGCAACGGTTCCAAAGGACAGAAGATTCTTCTGCTCACTAACCACTTCAGAGTCAACTTCAGAAAACCAAACAGTCATAATTTCTTCCATTACAGCGTTACTATCACATATGAAGATGGTAGTCCACTTTTAGCCAAAGGTTTTGGCAGAAAGATTCTTGAAAAAGTTCAACAGACTTGTCAAGCTGATTTGGGTTGCAAACATTTTGCTTATGATGGCGACAAGAATCTTTATACTGTTGGTCCTCTTCCTAGATCCAGTCTAGACTTCTCTGTTGTTCTTGAAACCGCGCCTTCTAGGAGAAACGCAGACAAAAGACTAAAGCTTCCTCACCAATCCAAGAAATTCAATGTTGCAATCCTTTTTGCGCCTCCAGAAATCCCAATGGAAGCTATTGCAAATGCTCTTCAAGGAAAGAAAACTAAACATCTTCTAGATGCTATCAGAGTGATGGACTGTATTTTGAGCCAAAATGCAGCTAGACAAGGTTGCCTCCTAGTTCGGCAATCTTTCTTCCACAATGACGCAAAGTACTTTGCGAATATCGGTGAAGGTGTAGATTGTTGCAAAGGATTTCATTCAAGCTTCAGAACTACTCAGGGAGGCTTGTCCCTCAATATTGATGTTTCGACTGCTATGATAGTAAAACCTGGTCCTGTTGTTGATTTTCTTATTGCAAATCAAGGTGTGAACGATCCATTCTCTATTAACTGGAAAAAGGCTAAAAATACTCTGAAAAATCTTAGAGTTAAAGTCCTCCCTTCAAATCAAGAATACAAGATAACCGGACTAAGTGGACTACACTGCAAAGATCAAACGTTTACTTGGAAGAAAAGGAACCAAAACAGGGAATTTGAGGAGGTTGAGATTACAGTGTCCGATTACTTCACTAGGATCCGTGAAATCGAACTGCGTTACTCGGGTGGCTTACCTTGTATCAATGTTGGTAAGCCAAATCGTCCTACCTACTTTCCCATTGAGCTCTGTGAGCTTGTATCTCTACAACGCTATACTAAAGCGCTAACCAAATTCCAGAGGAGTAACCTTATCAAAGAATCAAGGCAGAATCCACAACAAAGAATTGGTGTGTTAACCAGAGCTCTCAAGACTAGTAATTATAATGATGACCCAATGTTGCAAGAATGTGGTGTTAGGATTGGCTCTGACTTCACTCAAGTCGAAGGCCGCGTGTTACCAACACCAAAGCTTAAAGCTGGCAAAGAACAAGACATCTATCCTATTAATGGGAGTTGGAACTTCAAGAACAAGCCAGCCACAGTTACTAGATGGGCTGTTGTGAACTTCTCTGCTCGCTGTGACCCGCAAAAGATTATTGATGATTTGACCAGATGTGGAAAGATGAAAGGAATTAACGTAGATTCTCCATACCATGTCGTCTTTGAAGAAAATCCTCAGTTTAAGGATGCCACTGGTTCTGTAAGAGTTGATAAGATGTTTCAACATTTACAATCAATACTCGGCGAAGTTCCTCCAAAATTCCTTCTATGCATACTCGAAAAGAAAAACTCTGATGTTTATGAAAAATCTTGTTCAATGTGGAATTGTGAATGTATTGTTCCTCCTCAAAACTTAAATGATCAGTATCTCACAAATCTTCTACTAAAGATAAATGCCAAGCTTGGTGGATTGAATTCAGTTTTGGATATGGAGCTGTCAGGAACAATGCCTCTGGTAATGAGAGTTCCTACCATCATTATTGGAATGGATGTATCTCATGGTTCTCCTGGACAGTCTGATCATATACCATCCATTGCCGCGGTTGTGAGCTCCAGAGAGTGGCCACTGATCTCAAAATACAGGGCTTGTGTGCGTACACAGTCGCCTAAAGTTGAAATGATCGATAGCCTCTTTAAACCCGTCTCTGACAAAGATGATCAAGGTATCATGAGAGAGCTCTTGCTTGACTTTCACTCAAGTTCTGGAAAGAAACCGAATCACATTATCATTTTCAGGGATGGTGTCAGTGAATCTCAGTTTAACCAAGTTCTTAATATTGAACTGGATCAGATGATGCAAATAAACCACCACACGAAGTTCTTCCAAACCGAAAGCCCTAATAATGTTCTTCCAGGAACAATAATTGACAGCAACATCTGTCACCAACACAACAACGATTTCTATCTTTGTGCTCATGCTGGAAAGATTGGAACTACAAGGCCAACACATTACCATGTGCTCTACGACGAGATTGGATTTGACACAGATCAACTCCAAGAACTTGTGCATTCACTATCCTATGTCTACCAGCGGAGCACAACTGCCATCTCTCTTGTTGCGCCGATATGTTATGCTCATTTGGCGGCTGCACAGATGGCAACTGCAATGAAGTTTGAGGACATGTCTGAGACTTCATCGAGCCACGGCGGGATCACCACAGCTGGAGCAGTCCCTGTGCCTCCCATGCCGAAGCTGAACACCAACGTTGCTAGTTCAATGTTCTTTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000228 GO:0000785 GO:0000790 GO:0000791 GO:0002252 GO:0002376 GO:0003006 GO:0003674 GO:0003676 GO:0003682 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005719 GO:0005730 GO:0005737 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006355 GO:0006396 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006996 GO:0007049 GO:0007275 GO:0008150 GO:0008152 GO:0008213 GO:0009553 GO:0009554 GO:0009605 GO:0009607 GO:0009615 GO:0009617 GO:0009791 GO:0009814 GO:0009816 GO:0009889 GO:0009890 GO:0009892 GO:0009908 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010528 GO:0010529 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0015030 GO:0016032 GO:0016043 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016604 GO:0016787 GO:0016788 GO:0018022 GO:0018193 GO:0018205 GO:0019048 GO:0019219 GO:0019222 GO:0019538 GO:0019953 GO:0022402 GO:0022414 GO:0030154 GO:0030422 GO:0031047 GO:0031048 GO:0031050 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032259 GO:0032501 GO:0032502 GO:0034293 GO:0034641 GO:0034968 GO:0035194 GO:0035197 GO:0035198 GO:0035670 GO:0035821 GO:0036211 GO:0040029 GO:0042221 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0043934 GO:0044003 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044703 GO:0044728 GO:0045087 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048229 GO:0048236 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048519 GO:0048523 GO:0048533 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051321 GO:0051567 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0060255 GO:0061458 GO:0061647 GO:0061980 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0070919 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0071840 GO:0080090 GO:0080188 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0090567 GO:0097159 GO:0098542 GO:0099402 GO:0140098 GO:1901360 GO:1901363 GO:1901564 GO:1901698 GO:1901699 GO:1902679 GO:1903046 GO:1903506 GO:1903507 GO:1904159 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

850

Amino Acids

95.51

Weight (kDa)

8.96

Isoelectric Point (pI)

42.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArgoN PF16486 35 - 183 9.3e-29 N-terminal domain of argonaute
ArgoL1 PF08699 195 - 244 8.4e-17 Argonaute linker 1 domain
PAZ PF02170 249 - 377 1.2e-26 PAZ domain
ArgoL2 PF16488 388 - 432 8.9e-13 Argonaute linker 2 domain
Piwi PF02171 529 - 706 5.6e-37 Piwi domain
Piwi PF02171 707 - 811 5.4e-39 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1203
AccB7I CCANNNNNTGG 1 cut(s) 1881
AccI GTMKAC 1 cut(s) 2346
AccII CGCG 3 cut(s) 374, 1272, 1862
AciI CCGC 9 cut(s) 17, 372, 1270, 1406, 1860, 1862, 2353, 2405, 2469
AclI AACGTT 2 cut(s) 902, 2526
AclWI GGATC 6 cut(s) 333, 773, 973, 986, 2115, 2480
AcoI YGGCCR 1 cut(s) 1883
AcsI RAATTY 5 cut(s) 428, 935, 1112, 1580, 1735
AcuI CTGAAG 3 cut(s) 115, 127, 655
AfaI GTAC 2 cut(s) 617, 1918
AfeI AGCGCT 1 cut(s) 1104
AfiI CCNNNNNNNGG 3 cut(s) 931, 1328, 1881
AflIII ACRYGT 1 cut(s) 2442
AhlI ACTAGT 1 cut(s) 1193
AjnI CCWGG 3 cut(s) 733, 1828, 2173
AjuI GAANNNNNNNTTGG 2 cut(s) 1727, 1759
Alw21I GWGCWC 7 cut(s) 1068, 1186, 1874, 2001, 2236, 2283, 2360
Alw26I GTCTC 2 cut(s) 1969, 2444
AlwI GGATC 6 cut(s) 333, 773, 973, 986, 2115, 2480
AlwNI CAGNNNCTG 1 cut(s) 2495
Ama87I CYCGRG 1 cut(s) 1003
Aor51HI AGCGCT 1 cut(s) 1104
AoxI GGCC 3 cut(s) 1267, 1883, 2261
ApeKI GCWGC 2 cut(s) 560, 2408
ApoI RAATTY 5 cut(s) 428, 935, 1112, 1580, 1735
AseI ATTAAT 1 cut(s) 1326
Asp700I GAANNNNTTC 2 cut(s) 249, 2141
AspLEI GCGC 4 cut(s) 376, 454, 1105, 2383
AspS9I GGNCC 2 cut(s) 326, 737
AsuHPI GGTGA 4 cut(s) 407, 644, 2195, 2467
AvaI CYCGRG 1 cut(s) 1003
AvaII GGWCC 2 cut(s) 326, 737
BalI TGGCCA 1 cut(s) 1885
BamHI GGATCC 1 cut(s) 978
BanII GRGCYC 4 cut(s) 1068, 1186, 1874, 2001
BbsI GAAGAC 1 cut(s) 56
Bbv12I GWGCWC 7 cut(s) 1068, 1186, 1874, 2001, 2236, 2283, 2360
BbvI GCAGC 2 cut(s) 572, 2395
BccI CCATC 9 cut(s) 194, 293, 529, 1365, 1802, 1858, 2054, 2375, 2410
BceAI ACGGC 2 cut(s) 66, 2482
BciT130I CCWGG 3 cut(s) 735, 1830, 2175
BclI TGATCA 3 cut(s) 1681, 1840, 1978
BcoDI GTCTC 2 cut(s) 1969, 2444
BcuI ACTAGT 1 cut(s) 1193
BfoI RGCGCY 1 cut(s) 1106
BisI GCNGC 5 cut(s) 561, 1270, 1860, 2406, 2409
BlsI GCNGC 5 cut(s) 562, 1271, 1861, 2407, 2410
BmcAI AGTACT 1 cut(s) 617
Bme1390I CCNGG 3 cut(s) 735, 1830, 2175
Bme18I GGWCC 2 cut(s) 326, 737
BmeT110I CYCGRG 1 cut(s) 1003
BmgT120I GGNCC 2 cut(s) 326, 737
BmiI GGNNCC 3 cut(s) 94, 923, 980
BmrFI CCNGG 3 cut(s) 735, 1830, 2175
BmsI GCATC 3 cut(s) 511, 1498, 2106
BpiI GAAGAC 1 cut(s) 56
BpmI CTGGAG 3 cut(s) 441, 1858, 2505
BpuEI CTTGAG 3 cut(s) 1172, 1242, 2002
Bsa29I ATCGAT 1 cut(s) 1944
BsaBI GATNNNNATC 2 cut(s) 1686, 2223
BsaJI CCNNGG 3 cut(s) 82, 1860, 2464
BsaWI WCCGGW 1 cut(s) 869
Bsc4I CCNNNNNNNGG 3 cut(s) 931, 1328, 1881
Bse1I ACTGG 4 cut(s) 342, 800, 1519, 2109
Bse3DI GCAATG 2 cut(s) 1854, 2433
Bse8I GATNNNNATC 2 cut(s) 1686, 2223
BseBI CCWGG 3 cut(s) 735, 1830, 2175
BseCI ATCGAT 1 cut(s) 1944
BseDI CCNNGG 3 cut(s) 82, 1860, 2464
BseGI GGATG 4 cut(s) 1513, 1816, 1850, 2065
BseJI GATNNNNATC 2 cut(s) 1686, 2223
BseLI CCNNNNNNNGG 3 cut(s) 931, 1328, 1881
BseMI GCAATG 2 cut(s) 1854, 2433
BseMII CTCAG 4 cut(s) 695, 1511, 2090, 2439
BseNI ACTGG 4 cut(s) 342, 800, 1519, 2109
BseRI GAGGAG 4 cut(s) 12, 956, 1135, 1656
BseXI GCAGC 2 cut(s) 572, 2395
BsgI GTGCAG 1 cut(s) 2394
Bsh1236I CGCG 3 cut(s) 374, 1272, 1862
BshFI GGCC 3 cut(s) 1269, 1885, 2263
BshVI ATCGAT 1 cut(s) 1944
BsiHKAI GWGCWC 7 cut(s) 1068, 1186, 1874, 2001, 2236, 2283, 2360
BsiHKCI CYCGRG 1 cut(s) 1003
BsiSI CCGG 1 cut(s) 870
BslFI GGGAC 2 cut(s) 679, 2477
BslI CCNNNNNNNGG 3 cut(s) 931, 1328, 1881
BsmAI GTCTC 2 cut(s) 1969, 2444
BsmBI CGTCTC 1 cut(s) 1969
BsmFI GGGAC 2 cut(s) 679, 2477
BsmI GAATGC 1 cut(s) 2329
BsnI GGCC 3 cut(s) 1269, 1885, 2263
BsoBI CYCGRG 1 cut(s) 1003
Bsp1286I GDGCHC 7 cut(s) 1068, 1186, 1874, 2001, 2236, 2283, 2360
BspACI CCGC 9 cut(s) 17, 372, 1270, 1406, 1860, 1862, 2353, 2405, 2469
BspANI GGCC 3 cut(s) 1269, 1885, 2263
BspCNI CTCAG 4 cut(s) 694, 1510, 2089, 2440
BspDI ATCGAT 1 cut(s) 1944
BspFNI CGCG 3 cut(s) 374, 1272, 1862
BspHI TCATGA 1 cut(s) 1989
BspLI GGNNCC 3 cut(s) 94, 923, 980
BspPI GGATC 6 cut(s) 333, 773, 973, 986, 2115, 2480
BspQI GCTCTTC 2 cut(s) 44, 495
BsrDI GCAATG 2 cut(s) 1854, 2433
BsrI ACTGG 4 cut(s) 342, 800, 1519, 2109
BssECI CCNNGG 3 cut(s) 82, 1860, 2464
Bst2UI CCWGG 3 cut(s) 735, 1830, 2175
Bst4CI ACNGT 8 cut(s) 92, 158, 322, 542, 958, 1363, 1836, 1923
Bst6I CTCTTC 3 cut(s) 44, 336, 495
BstC8I GCNNGC 3 cut(s) 1300, 1356, 1396
BstDEI CTNAG 6 cut(s) 681, 827, 875, 1497, 2076, 2448
BstDSI CCRYGG 3 cut(s) 82, 1860, 2464
BstF5I GGATG 4 cut(s) 1513, 1816, 1850, 2065
BstFNI CGCG 3 cut(s) 374, 1272, 1862
BstH2I RGCGCY 1 cut(s) 1106
BstHHI GCGC 4 cut(s) 376, 454, 1105, 2383
BstMAI GTCTC 2 cut(s) 1969, 2444
BstMWI GCNNNNNNNGC 4 cut(s) 1072, 2402, 2417, 2506
BstNI CCWGG 3 cut(s) 735, 1830, 2175
BstNSI RCATGY 1 cut(s) 2446
BstSCI CCNGG 3 cut(s) 733, 1828, 2173
BstUI CGCG 3 cut(s) 374, 1272, 1862
BstV1I GCAGC 2 cut(s) 572, 2395
BstV2I GAAGAC 1 cut(s) 56
BstX2I RGATCY 2 cut(s) 338, 978
BstYI RGATCY 2 cut(s) 338, 978
Bsu15I ATCGAT 1 cut(s) 1944
BsuRI GGCC 3 cut(s) 1269, 1885, 2263
BsuTUI ATCGAT 1 cut(s) 1944
BtgI CCRYGG 3 cut(s) 82, 1860, 2464
BtsCI GGATG 4 cut(s) 1513, 1816, 1850, 2065
BtsI GCAGTG 1 cut(s) 886
BtsIMutI CAGTG 5 cut(s) 886, 963, 1512, 1886, 2074
Cac8I GCNNGC 3 cut(s) 1300, 1356, 1396
CaiI CAGNNNCTG 1 cut(s) 2495
CciI TCATGA 1 cut(s) 1989
CfoI GCGC 4 cut(s) 376, 454, 1105, 2383
Cfr13I GGNCC 2 cut(s) 326, 737
Cfr42I CCGCGG 1 cut(s) 1863
ClaI ATCGAT 1 cut(s) 1944
CseI GACGC 1 cut(s) 617
CsiI ACCWGGT 1 cut(s) 733
Csp6I GTAC 2 cut(s) 616, 1917
CspCI CAANNNNNGTGG 2 cut(s) 1708, 1743
CviAII CATG 8 cut(s) 31, 1475, 1820, 1990, 2237, 2276, 2443, 2506
CviQI GTAC 2 cut(s) 616, 1917
DdeI CTNAG 6 cut(s) 681, 827, 875, 1497, 2076, 2448
DraI TTTAAA 1 cut(s) 1957
EaeI YGGCCR 1 cut(s) 1883
Eam1104I CTCTTC 3 cut(s) 44, 336, 495
EarI CTCTTC 3 cut(s) 44, 336, 495
Ecl136II GAGCTC 4 cut(s) 1066, 1184, 1872, 1999
Eco24I GRGCYC 4 cut(s) 1068, 1186, 1874, 2001
Eco47I GGWCC 2 cut(s) 326, 737
Eco47III AGCGCT 1 cut(s) 1104
Eco53kI GAGCTC 4 cut(s) 1066, 1184, 1872, 1999
Eco57I CTGAAG 3 cut(s) 115, 127, 655
Eco88I CYCGRG 1 cut(s) 1003
EcoICRI GAGCTC 4 cut(s) 1066, 1184, 1872, 1999
EcoRI GAATTC 1 cut(s) 1735
EcoRII CCWGG 3 cut(s) 733, 1828, 2173
EcoT22I ATGCAT 1 cut(s) 1595
EcoT38I GRGCYC 4 cut(s) 1068, 1186, 1874, 2001
Esp3I CGTCTC 1 cut(s) 1969
FaeI CATG 8 cut(s) 34, 1478, 1823, 1993, 2240, 2279, 2446, 2509
FaqI GGGAC 2 cut(s) 679, 2477
FatI CATG 8 cut(s) 30, 1474, 1819, 1989, 2236, 2275, 2442, 2505
FauI CCCGC 2 cut(s) 1413, 2462
FauNDI CATATG 1 cut(s) 193
FbaI TGATCA 3 cut(s) 1681, 1840, 1978
FblI GTMKAC 1 cut(s) 2346
Fnu4HI GCNGC 5 cut(s) 561, 1270, 1860, 2406, 2409
FokI GGATG 4 cut(s) 1520, 1823, 1837, 2072
FriOI GRGCYC 4 cut(s) 1068, 1186, 1874, 2001
Fsp4HI GCNGC 5 cut(s) 561, 1270, 1860, 2406, 2409
GlaI GCGC 4 cut(s) 375, 453, 1104, 2382
GluI GCNGC 5 cut(s) 561, 1270, 1860, 2406, 2409
GsuI CTGGAG 3 cut(s) 441, 1858, 2505
HaeII RGCGCY 1 cut(s) 1106
HaeIII GGCC 3 cut(s) 1269, 1885, 2263
HapII CCGG 1 cut(s) 870
HgaI GACGC 1 cut(s) 617
HhaI GCGC 4 cut(s) 376, 454, 1105, 2383
Hin1II CATG 8 cut(s) 34, 1478, 1823, 1993, 2240, 2279, 2446, 2509
Hin6I GCGC 4 cut(s) 374, 452, 1103, 2381
HinP1I GCGC 4 cut(s) 374, 452, 1103, 2381
HincII GTYRAC 2 cut(s) 139, 1176
HindII GTYRAC 2 cut(s) 139, 1176
HindIII AAGCTT 4 cut(s) 407, 667, 1289, 1721
HpaI GTTAAC 1 cut(s) 1176
HpaII CCGG 1 cut(s) 870
HphI GGTGA 4 cut(s) 407, 644, 2195, 2467
Hpy99I CGWCG 1 cut(s) 2291
HpyAV CCTTC 5 cut(s) 387, 629, 855, 1259, 1595
HpyCH4III ACNGT 8 cut(s) 92, 158, 322, 542, 958, 1363, 1836, 1923
HpyCH4IV ACGT 4 cut(s) 37, 902, 1458, 2526
HpyF10VI GCNNNNNNNGC 4 cut(s) 1072, 2402, 2417, 2506
HpyF3I CTNAG 6 cut(s) 681, 827, 875, 1497, 2076, 2448
HpySE526I ACGT 4 cut(s) 37, 902, 1458, 2526
Hsp92II CATG 8 cut(s) 34, 1478, 1823, 1993, 2240, 2279, 2446, 2509
HspAI GCGC 4 cut(s) 374, 452, 1103, 2381
Ksp22I TGATCA 3 cut(s) 1681, 1840, 1978
KspAI GTTAAC 1 cut(s) 1176
KspI CCGCGG 1 cut(s) 1863
LguI GCTCTTC 2 cut(s) 44, 495
LmnI GCTCC 4 cut(s) 1753, 1877, 2355, 2486
Lsp1109I GCAGC 2 cut(s) 572, 2395
LweI GCATC 3 cut(s) 511, 1498, 2106
MabI ACCWGGT 1 cut(s) 733
MaeII ACGT 4 cut(s) 37, 902, 1458, 2526
MaeIII GTNAC 7 cut(s) 181, 998, 1124, 1275, 1363, 1400, 2201
MflI RGATCY 2 cut(s) 338, 978
MhlI GDGCHC 7 cut(s) 1068, 1186, 1874, 2001, 2236, 2283, 2360
MlsI TGGCCA 1 cut(s) 1885
MluNI TGGCCA 1 cut(s) 1885
MlyI GAGTC 3 cut(s) 4, 70, 144
MmeI TCCRAC 1 cut(s) 1316
Mox20I TGGCCA 1 cut(s) 1885
Mph1103I ATGCAT 1 cut(s) 1595
MroXI GAANNNNTTC 2 cut(s) 249, 2141
MscI TGGCCA 1 cut(s) 1885
MslI CAYNNNNRTG 1 cut(s) 2274
Msp20I TGGCCA 1 cut(s) 1885
MspA1I CMGCKG 3 cut(s) 1862, 2353, 2483
MspI CCGG 1 cut(s) 870
MspR9I CCNGG 3 cut(s) 735, 1830, 2175
Mva1269I GAATGC 1 cut(s) 2329
MvaI CCWGG 3 cut(s) 735, 1830, 2175
MvnI CGCG 3 cut(s) 374, 1272, 1862
MwoI GCNNNNNNNGC 4 cut(s) 1072, 2402, 2417, 2506
NdeI CATATG 1 cut(s) 193
NlaIII CATG 8 cut(s) 34, 1478, 1823, 1993, 2240, 2279, 2446, 2509
NlaIV GGNNCC 3 cut(s) 94, 923, 980
NmeAIII GCCGAG 1 cut(s) 1542
NmuCI GTSAC 2 cut(s) 1400, 2201
NsiI ATGCAT 1 cut(s) 1595
NspI RCATGY 1 cut(s) 2446
PagI TCATGA 1 cut(s) 1989
PciI ACATGT 1 cut(s) 2442
PciSI GCTCTTC 2 cut(s) 44, 495
PcsI WCGNNNNNNNCGW 1 cut(s) 1269
PctI GAATGC 1 cut(s) 2329
PdmI GAANNNNTTC 2 cut(s) 249, 2141
PfeI GAWTC 8 cut(s) 109, 238, 310, 1139, 1150, 1463, 2038, 2072
PflMI CCANNNNNTGG 1 cut(s) 1881
PfoI TCCNGGA 2 cut(s) 1828, 2173
PkrI GCNGC 5 cut(s) 562, 1271, 1861, 2407, 2410
PleI GAGTC 3 cut(s) 4, 70, 143
PpsI GAGTC 3 cut(s) 4, 70, 143
PscI ACATGT 1 cut(s) 2442
PshBI ATTAAT 1 cut(s) 1326
PsiI TTATAA 1 cut(s) 1203
Psp124BI GAGCTC 4 cut(s) 1068, 1186, 1874, 2001
Psp1406I AACGTT 2 cut(s) 902, 2526
Psp6I CCWGG 3 cut(s) 733, 1828, 2173
PspGI CCWGG 3 cut(s) 733, 1828, 2173
PspN4I GGNNCC 3 cut(s) 94, 923, 980
PspPI GGNCC 2 cut(s) 326, 737
PsrI GAACNNNNNNTAC 2 cut(s) 1770, 1802
PstNI CAGNNNCTG 1 cut(s) 2495
PsuI RGATCY 2 cut(s) 338, 978
PvuII CAGCTG 1 cut(s) 2483
RsaI GTAC 2 cut(s) 617, 1918
RsaNI GTAC 2 cut(s) 616, 1917
RseI CAYNNNNRTG 1 cut(s) 2274
SacI GAGCTC 4 cut(s) 1068, 1186, 1874, 2001
SacII CCGCGG 1 cut(s) 1863
SapI GCTCTTC 2 cut(s) 44, 495
SatI GCNGC 5 cut(s) 561, 1270, 1860, 2406, 2409
Sau96I GGNCC 2 cut(s) 326, 737
ScaI AGTACT 1 cut(s) 617
SchI GAGTC 3 cut(s) 4, 70, 144
ScrFI CCNGG 3 cut(s) 735, 1830, 2175
SduI GDGCHC 7 cut(s) 1068, 1186, 1874, 2001, 2236, 2283, 2360
SexAI ACCWGGT 1 cut(s) 733
SfaNI GCATC 3 cut(s) 511, 1498, 2106
Sfr303I CCGCGG 1 cut(s) 1863
SgrBI CCGCGG 1 cut(s) 1863
SinI GGWCC 2 cut(s) 326, 737
SmiMI CAYNNNNRTG 1 cut(s) 2274
SmlI CTYRAG 3 cut(s) 1187, 1257, 2017
SmoI CTYRAG 3 cut(s) 1187, 1257, 2017
SpeI ACTAGT 1 cut(s) 1193
SsiI CCGC 9 cut(s) 17, 372, 1270, 1406, 1860, 1862, 2353, 2405, 2469
SspI AATATT 2 cut(s) 703, 2098
SstI GAGCTC 4 cut(s) 1068, 1186, 1874, 2001
StyD4I CCNGG 3 cut(s) 733, 1828, 2173
TaaI ACNGT 8 cut(s) 92, 158, 322, 542, 958, 1363, 1836, 1923
TaiI ACGT 4 cut(s) 40, 905, 1461, 2529
TaqI TCGA 6 cut(s) 713, 990, 1263, 1599, 1944, 2459
TatI WGTACW 1 cut(s) 615
TauI GCSGC 3 cut(s) 1272, 1862, 2408
TfiI GAWTC 8 cut(s) 109, 238, 310, 1139, 1150, 1463, 2038, 2072
TscAI CASTG 5 cut(s) 893, 963, 1519, 1893, 2074
TseFI GTSAC 2 cut(s) 1400, 2201
TseI GCWGC 2 cut(s) 560, 2408
Tsp45I GTSAC 2 cut(s) 1400, 2201
TspDTI ATGAA 6 cut(s) 210, 650, 1460, 1638, 2444, 2445
TspGWI ACGGA 1 cut(s) 971
TspRI CASTG 5 cut(s) 893, 963, 1519, 1893, 2074
Van91I CCANNNNNTGG 1 cut(s) 1881
VpaK11BI GGWCC 2 cut(s) 326, 737
VspI ATTAAT 1 cut(s) 1326
XapI RAATTY 5 cut(s) 428, 935, 1112, 1580, 1735
XbaI TCTAGA 2 cut(s) 346, 517
XceI RCATGY 1 cut(s) 2446
XmiI GTMKAC 1 cut(s) 2346
XmnI GAANNNNTTC 2 cut(s) 249, 2141
ZrmI AGTACT 1 cut(s) 617
Zsp2I ATGCAT 1 cut(s) 1595
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.