Rroxscaffold_7G00201270

Argonaute

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0001623
Physical Location & Seq
Forward (+)
48853387 .. 48859379
5993 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00201270.1

Sequence Viewer

Length: 480 bp
ATGCTATACCAAGGCTTTATCCAATCTCAAAGGGCTTCACTAGTGGAGAAGTCTCGGCAGAAACCCCAGGAGCGAATGTCAACTTTGCGTGATCTTGGTGGAATCAACTCCTTGCTAATGGTGGAGCATTCTCCTTCTCTGCCCTTGGTATCCAAGTCCCCCACTCTGATTTTAGGGATGGATGTGTCACATGGCTCACCTGGGCGTTCCGATGTGCCTTCTATTGCAGCGCCTTCGGACAACGGGGAAGCATATAAGCCCCCGTTGTCCGGCACACATTCGGACAACGCCAACTGTGGGAATGAGGTCTTGGCAGTTGGAAAGAGAAATATTGATGCTGCAACTATGGGTTTGACCACTTTAGAGCCAACCGTGGCTAATTCGGGGAAGAGGAAGAGAGGTCATCCTGTTGGTAGCCAAAACAAAATGTCGTATGGCACTACCGGGAAATGTAAGAAAGGCAAGGAAGCGAGGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

159

Amino Acids

16.93

Weight (kDa)

9.85

Isoelectric Point (pI)

56.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Piwi PF02171 32 - 77 1.6e-06 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfiI CCNNNNNNNGG 2 cut(s) 269, 297
AhlI ACTAGT 1 cut(s) 40
AjnI CCWGG 2 cut(s) 66, 199
AjuI GAANNNNNNNTTGG 2 cut(s) 293, 325
Alw26I GTCTC 1 cut(s) 57
ApeKI GCWGC 2 cut(s) 227, 338
AspLEI GCGC 1 cut(s) 232
AsuC2I CCSGG 1 cut(s) 445
AsuHPI GGTGA 1 cut(s) 189
BbvI GCAGC 2 cut(s) 239, 325
BccI CCATC 1 cut(s) 172
BcgI CGANNNNNNTGC 2 cut(s) 216, 250
BciT130I CCWGG 2 cut(s) 68, 201
BciVI GTATCC 1 cut(s) 160
BcnI CCSGG 1 cut(s) 445
BcoDI GTCTC 1 cut(s) 57
BcuI ACTAGT 1 cut(s) 40
BfaI CTAG 1 cut(s) 41
BfoI RGCGCY 1 cut(s) 233
BfuI GTATCC 1 cut(s) 160
BisI GCNGC 2 cut(s) 228, 339
BlsI GCNGC 2 cut(s) 229, 340
Bme1390I CCNGG 3 cut(s) 68, 201, 445
BmrFI CCNGG 3 cut(s) 68, 201, 445
BmsI GCATC 1 cut(s) 325
BpuMI CCSGG 1 cut(s) 445
BsaJI CCNNGG 5 cut(s) 10, 66, 144, 200, 372
BsaXI ACNNNNNCTCC 2 cut(s) 62, 92
Bsc4I CCNNNNNNNGG 2 cut(s) 269, 297
BseBI CCWGG 2 cut(s) 68, 201
BseDI CCNNGG 5 cut(s) 10, 66, 144, 200, 372
BseGI GGATG 3 cut(s) 183, 187, 403
BseLI CCNNNNNNNGG 2 cut(s) 269, 297
BseXI GCAGC 2 cut(s) 239, 325
BsiSI CCGG 2 cut(s) 270, 444
BslFI GGGAC 1 cut(s) 142
BslI CCNNNNNNNGG 2 cut(s) 269, 297
BsmAI GTCTC 1 cut(s) 57
BsmFI GGGAC 1 cut(s) 142
BsmI GAATGC 1 cut(s) 127
Bsp143I GATC 1 cut(s) 91
BssECI CCNNGG 5 cut(s) 10, 66, 144, 200, 372
BssMI GATC 1 cut(s) 91
BssT1I CCWWGG 2 cut(s) 10, 144
Bst2UI CCWGG 2 cut(s) 68, 201
Bst4CI ACNGT 2 cut(s) 296, 373
Bst6I CTCTTC 2 cut(s) 383, 389
BstDSI CCRYGG 1 cut(s) 372
BstF5I GGATG 3 cut(s) 183, 187, 403
BstH2I RGCGCY 1 cut(s) 233
BstHHI GCGC 1 cut(s) 232
BstKTI GATC 1 cut(s) 94
BstMAI GTCTC 1 cut(s) 57
BstMBI GATC 1 cut(s) 91
BstNI CCWGG 2 cut(s) 68, 201
BstSCI CCNGG 3 cut(s) 66, 199, 443
BstV1I GCAGC 2 cut(s) 239, 325
BsuI GTATCC 1 cut(s) 160
BtgI CCRYGG 1 cut(s) 372
BtsCI GGATG 3 cut(s) 183, 187, 403
CfoI GCGC 1 cut(s) 232
CviAII CATG 1 cut(s) 191
CviJI RGCY 7 cut(s) 15, 35, 195, 259, 367, 377, 417
CviKI_1 RGCY 7 cut(s) 15, 35, 195, 259, 367, 377, 417
DpnI GATC 1 cut(s) 93
DpnII GATC 1 cut(s) 91
Eam1104I CTCTTC 2 cut(s) 383, 389
EarI CTCTTC 2 cut(s) 383, 389
Eco130I CCWWGG 2 cut(s) 10, 144
EcoRII CCWGG 2 cut(s) 66, 199
EcoT14I CCWWGG 2 cut(s) 10, 144
ErhI CCWWGG 2 cut(s) 10, 144
FaeI CATG 1 cut(s) 194
FaiI YATR 6 cut(s) 7, 192, 253, 255, 347, 435
FaqI GGGAC 1 cut(s) 142
FatI CATG 1 cut(s) 190
Fnu4HI GCNGC 2 cut(s) 228, 339
FokI GGATG 3 cut(s) 190, 194, 390
Fsp4HI GCNGC 2 cut(s) 228, 339
FspBI CTAG 1 cut(s) 41
GlaI GCGC 1 cut(s) 231
GluI GCNGC 2 cut(s) 228, 339
HaeII RGCGCY 1 cut(s) 233
HapII CCGG 2 cut(s) 270, 444
HhaI GCGC 1 cut(s) 232
Hin1II CATG 1 cut(s) 194
Hin6I GCGC 1 cut(s) 230
HinP1I GCGC 1 cut(s) 230
HincII GTYRAC 1 cut(s) 81
HindII GTYRAC 1 cut(s) 81
HinfI GANTC 1 cut(s) 102
HpaII CCGG 2 cut(s) 270, 444
HphI GGTGA 1 cut(s) 189
Hpy166II GTNNAC 1 cut(s) 81
Hpy188I TCNGA 4 cut(s) 168, 211, 238, 283
Hpy8I GTNNAC 1 cut(s) 81
HpyAV CCTTC 3 cut(s) 144, 228, 243
HpyCH4III ACNGT 2 cut(s) 296, 373
HpyCH4V TGCA 2 cut(s) 227, 341
Hsp92II CATG 1 cut(s) 194
HspAI GCGC 1 cut(s) 230
Kzo9I GATC 1 cut(s) 91
LmnI GCTCC 2 cut(s) 70, 124
LpnPI CCDG 7 cut(s) 53, 80, 186, 213, 283, 420, 457
Lsp1109I GCAGC 2 cut(s) 239, 325
LweI GCATC 1 cut(s) 325
MaeI CTAG 1 cut(s) 41
MaeIII GTNAC 1 cut(s) 186
MalI GATC 1 cut(s) 93
MboI GATC 1 cut(s) 91
MboII GAAGA 2 cut(s) 400, 406
MluCI AATT 1 cut(s) 379
MmeI TCCRAC 1 cut(s) 298
MnlI CCTC 5 cut(s) 298, 384, 392, 465, 468
MspI CCGG 2 cut(s) 270, 444
MspR9I CCNGG 3 cut(s) 68, 201, 445
Mva1269I GAATGC 1 cut(s) 127
MvaI CCWGG 2 cut(s) 68, 201
NciI CCSGG 1 cut(s) 445
NdeII GATC 1 cut(s) 91
NlaIII CATG 1 cut(s) 194
NmeAIII GCCGAG 1 cut(s) 34
NmuCI GTSAC 1 cut(s) 186
PctI GAATGC 1 cut(s) 127
PfeI GAWTC 1 cut(s) 102
PkrI GCNGC 2 cut(s) 229, 340
Psp6I CCWGG 2 cut(s) 66, 199
PspGI CCWGG 2 cut(s) 66, 199
SatI GCNGC 2 cut(s) 228, 339
Sau3AI GATC 1 cut(s) 91
ScrFI CCNGG 3 cut(s) 68, 201, 445
SetI ASST 4 cut(s) 202, 309, 403, 479
SfaNI GCATC 1 cut(s) 325
SpeI ACTAGT 1 cut(s) 40
Sse9I AATT 1 cut(s) 379
SspI AATATT 1 cut(s) 331
SspMI CTAG 1 cut(s) 41
StyD4I CCNGG 3 cut(s) 66, 199, 443
StyI CCWWGG 2 cut(s) 10, 144
TaaI ACNGT 2 cut(s) 296, 373
TasI AATT 1 cut(s) 379
TfiI GAWTC 1 cut(s) 102
TseFI GTSAC 1 cut(s) 186
TseI GCWGC 2 cut(s) 227, 338
Tsp45I GTSAC 1 cut(s) 186
XspI CTAG 1 cut(s) 41
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.