Rh4BG175500

gene silencing by RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0001623
Physical Location & Seq
Reverse (-)
31232895 .. 31235897
3003 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG175500.1

Sequence Viewer

Length: 801 bp
ATGCTTCGTTCTGCTGGAATATCGATTGGTACAGAACTCATGCAGGTTGAAGGTCGTGTGTTGTCAGCCCCAAATTTGAAAGTGGGAAATGGAGATGTCATTTTTCCTAGGAATGGGAGATGGAATTTCAACAATAAAAAACTAGTAGATCCTGTAAAACTAGAACGCTGGGCTGTTGTGAATTTTTCTGCTCGCTGTGACATTCGGTACCTGGTTGACACTATGATAAAGTGTGGAGATATGAAAGGAATTTCCATCAAGCCTCCATTTGGTGTGTTTGAAGAGAGTATTCAGAACAGACGTCAGTCAGCTCCGGTTAGAGTAGAAAAGATGTTTGAATACATTAAGCCCAAACTTCCAGGACCACCACAGTTTTTGTTGTGCATTCTCCCAGAGAGGAAGAATTCTGAGATATATGGTCCATGGAAAAGGAAAAACCTTTCAGACATTGGGATTGCAACGCAGTGCATTGCACCTACGAAGGTGAACGATCAATACATCACTAACGTGCTCCTGAAAATTAATGCAAAGCTAGGTGGAATGAACTCCTTGCTAACTATGGAGCATTCTCCTTCTCTACCCTTGGTTTCCAAGTCCCCTACTCTGATATTAGGGATGGATGTGTCACATGGCTCACCTGGGCAGTCAAATATGCCTTCTATTGCAGCAGTGTCGTTGAACCTGCCAGTAGACCTAAGAGGACAGCCCCTCAATCCCGCACACTTAAGCCAGCTGAGAGCCCCGAATCTGACACCTCTAGCCCCACTGAAAGCAATAGAAAGGGGTCCTGAGCCTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

266

Amino Acids

29.32

Weight (kDa)

9.64

Isoelectric Point (pI)

45.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArgoL2 PF16488 2 - 27 3.8e-07 Argonaute linker 2 domain
ArgoMid PF16487 36 - 87 1.8e-06 Mid domain of argonaute
Piwi PF02171 126 - 228 5.8e-25 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 304
Acc36I ACCTGC 2 cut(s) 34, 690
Acc65I GGTACC 1 cut(s) 207
AccB1I GGYRCC 1 cut(s) 207
AccI GTMKAC 1 cut(s) 690
AciI CCGC 1 cut(s) 717
AclWI GGATC 1 cut(s) 143
AcsI RAATTY 5 cut(s) 73, 124, 181, 249, 403
AcyI GRCGYC 1 cut(s) 301
AfaI GTAC 2 cut(s) 31, 209
AfiI CCNNNNNNNGG 2 cut(s) 113, 269
AflII CTTAAG 1 cut(s) 724
AgsI TTSAA 6 cut(s) 50, 79, 130, 281, 338, 679
AhlI ACTAGT 1 cut(s) 142
AjnI CCWGG 3 cut(s) 210, 358, 637
AleI CACNNNNGTG 1 cut(s) 506
AluBI AGCT 3 cut(s) 311, 532, 733
AluI AGCT 3 cut(s) 311, 532, 733
Alw21I GWGCWC 1 cut(s) 513
AlwI GGATC 1 cut(s) 143
ApeKI GCWGC 1 cut(s) 665
ApoI RAATTY 5 cut(s) 73, 124, 181, 249, 403
AseI ATTAAT 1 cut(s) 522
Asp718I GGTACC 1 cut(s) 207
AspA2I CCTAGG 1 cut(s) 107
AspS9I GGNCC 3 cut(s) 362, 419, 785
AsuHPI GGTGA 2 cut(s) 496, 627
AvaII GGWCC 3 cut(s) 362, 419, 785
AvrII CCTAGG 1 cut(s) 107
BanI GGYRCC 1 cut(s) 207
BanII GRGCYC 1 cut(s) 742
Bbv12I GWGCWC 1 cut(s) 513
BbvI GCAGC 1 cut(s) 677
BccI CCATC 3 cut(s) 114, 263, 610
BcgI CGANNNNNNTGC 2 cut(s) 654, 688
BciT130I CCWGG 3 cut(s) 212, 360, 639
BcuI ACTAGT 1 cut(s) 142
BfaI CTAG 5 cut(s) 108, 143, 161, 533, 758
BfrI CTTAAG 1 cut(s) 724
BfuAI ACCTGC 2 cut(s) 34, 690
BisI GCNGC 1 cut(s) 666
BlnI CCTAGG 1 cut(s) 107
BlsI GCNGC 1 cut(s) 667
Bme1390I CCNGG 3 cut(s) 212, 360, 639
Bme18I GGWCC 3 cut(s) 362, 419, 785
BmgT120I GGNCC 3 cut(s) 362, 419, 785
BmiI GGNNCC 2 cut(s) 209, 786
BmrFI CCNGG 3 cut(s) 212, 360, 639
BoxI GACNNNNGTC 1 cut(s) 304
Bpu10I CCTNAGC 1 cut(s) 789
Bsa29I ATCGAT 1 cut(s) 23
BsaHI GRCGYC 1 cut(s) 301
BsaJI CCNNGG 4 cut(s) 107, 422, 582, 638
BsaWI WCCGGW 1 cut(s) 313
Bsc4I CCNNNNNNNGG 2 cut(s) 113, 269
Bse1I ACTGG 1 cut(s) 686
Bse3DI GCAATG 1 cut(s) 468
BseBI CCWGG 3 cut(s) 212, 360, 639
BseCI ATCGAT 1 cut(s) 23
BseDI CCNNGG 4 cut(s) 107, 422, 582, 638
BseGI GGATG 2 cut(s) 621, 625
BseLI CCNNNNNNNGG 2 cut(s) 113, 269
BseMI GCAATG 1 cut(s) 468
BseMII CTCAG 4 cut(s) 399, 725, 780, 786
BseNI ACTGG 1 cut(s) 686
BseXI GCAGC 1 cut(s) 677
BseYI CCCAGC 1 cut(s) 168
BshNI GGYRCC 1 cut(s) 207
BshVI ATCGAT 1 cut(s) 23
BsiHKAI GWGCWC 1 cut(s) 513
BsiSI CCGG 1 cut(s) 314
BslFI GGGAC 1 cut(s) 580
BslI CCNNNNNNNGG 2 cut(s) 113, 269
BsmFI GGGAC 1 cut(s) 580
BsmI GAATGC 2 cut(s) 384, 565
Bsp1286I GDGCHC 2 cut(s) 513, 742
Bsp143I GATC 2 cut(s) 148, 490
Bsp19I CCATGG 1 cut(s) 422
BspACI CCGC 1 cut(s) 717
BspCNI CTCAG 4 cut(s) 400, 726, 781, 787
BspDI ATCGAT 1 cut(s) 23
BspLI GGNNCC 2 cut(s) 209, 786
BspMI ACCTGC 2 cut(s) 34, 690
BspPI GGATC 1 cut(s) 143
BspT107I GGYRCC 1 cut(s) 207
BspTI CTTAAG 1 cut(s) 724
BsrDI GCAATG 1 cut(s) 468
BsrI ACTGG 1 cut(s) 686
BssECI CCNNGG 4 cut(s) 107, 422, 582, 638
BssMI GATC 2 cut(s) 148, 490
BssNI GRCGYC 1 cut(s) 301
BssT1I CCWWGG 3 cut(s) 107, 422, 582
Bst2UI CCWGG 3 cut(s) 212, 360, 639
Bst4CI ACNGT 1 cut(s) 372
Bst6I CTCTTC 1 cut(s) 276
BstACI GRCGYC 1 cut(s) 301
BstAFI CTTAAG 1 cut(s) 724
BstC8I GCNNGC 2 cut(s) 193, 731
BstDEI CTNAG 5 cut(s) 408, 695, 734, 789, 795
BstDSI CCRYGG 1 cut(s) 422
BstF5I GGATG 2 cut(s) 621, 625
BstKTI GATC 2 cut(s) 151, 493
BstMBI GATC 2 cut(s) 148, 490
BstNI CCWGG 3 cut(s) 212, 360, 639
BstPAI GACNNNNGTC 1 cut(s) 304
BstSCI CCNGG 3 cut(s) 210, 358, 637
BstV1I GCAGC 1 cut(s) 677
BstX2I RGATCY 1 cut(s) 148
BstYI RGATCY 1 cut(s) 148
Bsu15I ATCGAT 1 cut(s) 23
BsuTUI ATCGAT 1 cut(s) 23
BtgI CCRYGG 1 cut(s) 422
BtsCI GGATG 2 cut(s) 621, 625
BtsI GCAGTG 2 cut(s) 470, 675
BtsIMutI CAGTG 3 cut(s) 470, 675, 764
BveI ACCTGC 2 cut(s) 34, 690
Cac8I GCNNGC 2 cut(s) 193, 731
Cfr13I GGNCC 3 cut(s) 362, 419, 785
ClaI ATCGAT 1 cut(s) 23
CsiI ACCWGGT 1 cut(s) 210
Csp6I GTAC 2 cut(s) 30, 208
CspCI CAANNNNNGTGG 2 cut(s) 357, 392
CviAII CATG 3 cut(s) 40, 423, 629
CviQI GTAC 2 cut(s) 30, 208
DdeI CTNAG 5 cut(s) 408, 695, 734, 789, 795
DpnI GATC 2 cut(s) 150, 492
DpnII GATC 2 cut(s) 148, 490
Eam1104I CTCTTC 1 cut(s) 276
EarI CTCTTC 1 cut(s) 276
Eco130I CCWWGG 3 cut(s) 107, 422, 582
Eco24I GRGCYC 1 cut(s) 742
Eco47I GGWCC 3 cut(s) 362, 419, 785
EcoO109I RGGNCCY 1 cut(s) 785
EcoRI GAATTC 1 cut(s) 403
EcoRII CCWGG 3 cut(s) 210, 358, 637
EcoT14I CCWWGG 3 cut(s) 107, 422, 582
EcoT38I GRGCYC 1 cut(s) 742
ErhI CCWWGG 3 cut(s) 107, 422, 582
FaeI CATG 3 cut(s) 43, 426, 632
FaiI YATR 9 cut(s) 41, 224, 242, 415, 417, 424, 560, 630, 653
FaqI GGGAC 1 cut(s) 580
FatI CATG 3 cut(s) 39, 422, 628
FauI CCCGC 1 cut(s) 724
FblI GTMKAC 1 cut(s) 690
Fnu4HI GCNGC 1 cut(s) 666
FokI GGATG 2 cut(s) 628, 632
FriOI GRGCYC 1 cut(s) 742
Fsp4HI GCNGC 1 cut(s) 666
FspBI CTAG 5 cut(s) 108, 143, 161, 533, 758
GluI GCNGC 1 cut(s) 666
GsaI CCCAGC 1 cut(s) 172
HapII CCGG 1 cut(s) 314
Hin1I GRCGYC 1 cut(s) 301
Hin1II CATG 3 cut(s) 43, 426, 632
HincII GTYRAC 1 cut(s) 217
HindII GTYRAC 1 cut(s) 217
HinfI GANTC 1 cut(s) 745
HpaII CCGG 1 cut(s) 314
HphI GGTGA 2 cut(s) 496, 627
Hpy166II GTNNAC 3 cut(s) 217, 487, 691
Hpy188I TCNGA 5 cut(s) 294, 409, 445, 606, 750
Hpy188III TCNNGA 2 cut(s) 514, 788
Hpy8I GTNNAC 3 cut(s) 217, 487, 691
HpyAV CCTTC 4 cut(s) 44, 475, 582, 666
HpyCH4III ACNGT 1 cut(s) 372
HpyCH4IV ACGT 2 cut(s) 301, 507
HpyCH4V TGCA 7 cut(s) 43, 384, 458, 468, 473, 527, 665
HpyF3I CTNAG 5 cut(s) 408, 695, 734, 789, 795
HpySE526I ACGT 2 cut(s) 301, 507
Hsp92I GRCGYC 1 cut(s) 301
Hsp92II CATG 3 cut(s) 43, 426, 632
KpnI GGTACC 1 cut(s) 211
Kzo9I GATC 2 cut(s) 148, 490
LmnI GCTCC 3 cut(s) 316, 516, 562
Lsp1109I GCAGC 1 cut(s) 677
MabI ACCWGGT 1 cut(s) 210
MaeI CTAG 5 cut(s) 108, 143, 161, 533, 758
MaeII ACGT 2 cut(s) 301, 507
MaeIII GTNAC 2 cut(s) 197, 624
MalI GATC 2 cut(s) 150, 492
MboI GATC 2 cut(s) 148, 490
MboII GAAGA 2 cut(s) 293, 412
MflI RGATCY 1 cut(s) 148
MhlI GDGCHC 2 cut(s) 513, 742
MluCI AATT 6 cut(s) 73, 124, 181, 249, 403, 519
MnlI CCTC 5 cut(s) 273, 390, 692, 719, 765
MseI TTAA 3 cut(s) 345, 522, 725
MslI CAYNNNNRTG 1 cut(s) 506
MspA1I CMGCKG 1 cut(s) 733
MspCI CTTAAG 1 cut(s) 724
MspI CCGG 1 cut(s) 314
MspR9I CCNGG 3 cut(s) 212, 360, 639
Mva1269I GAATGC 2 cut(s) 384, 565
MvaI CCWGG 3 cut(s) 212, 360, 639
NcoI CCATGG 1 cut(s) 422
NdeII GATC 2 cut(s) 148, 490
NlaIII CATG 3 cut(s) 43, 426, 632
NlaIV GGNNCC 2 cut(s) 209, 786
NmuCI GTSAC 2 cut(s) 197, 624
OliI CACNNNNGTG 1 cut(s) 506
PctI GAATGC 2 cut(s) 384, 565
PfeI GAWTC 1 cut(s) 745
PfoI TCCNGGA 1 cut(s) 358
PkrI GCNGC 1 cut(s) 667
PpuMI RGGWCCY 1 cut(s) 785
PshAI GACNNNNGTC 1 cut(s) 304
PshBI ATTAAT 1 cut(s) 522
Psp5II RGGWCCY 1 cut(s) 785
Psp6I CCWGG 3 cut(s) 210, 358, 637
PspFI CCCAGC 1 cut(s) 168
PspGI CCWGG 3 cut(s) 210, 358, 637
PspN4I GGNNCC 2 cut(s) 209, 786
PspPI GGNCC 3 cut(s) 362, 419, 785
PspPPI RGGWCCY 1 cut(s) 785
PsrI GAACNNNNNNTAC 2 cut(s) 479, 511
PsuI RGATCY 1 cut(s) 148
PvuII CAGCTG 1 cut(s) 733
RsaI GTAC 2 cut(s) 31, 209
RsaNI GTAC 2 cut(s) 30, 208
RseI CAYNNNNRTG 1 cut(s) 506
SaqAI TTAA 3 cut(s) 345, 522, 725
SatI GCNGC 1 cut(s) 666
Sau3AI GATC 2 cut(s) 148, 490
Sau96I GGNCC 3 cut(s) 362, 419, 785
ScrFI CCNGG 3 cut(s) 212, 360, 639
SduI GDGCHC 2 cut(s) 513, 742
SexAI ACCWGGT 1 cut(s) 210
SinI GGWCC 3 cut(s) 362, 419, 785
SmiMI CAYNNNNRTG 1 cut(s) 506
SmlI CTYRAG 1 cut(s) 724
SmoI CTYRAG 1 cut(s) 724
SpeI ACTAGT 1 cut(s) 142
Sse9I AATT 6 cut(s) 73, 124, 181, 249, 403, 519
SsiI CCGC 1 cut(s) 717
SspMI CTAG 5 cut(s) 108, 143, 161, 533, 758
StyD4I CCNGG 3 cut(s) 210, 358, 637
StyI CCWWGG 3 cut(s) 107, 422, 582
TaaI ACNGT 1 cut(s) 372
TaiI ACGT 2 cut(s) 304, 510
TaqI TCGA 1 cut(s) 23
TasI AATT 6 cut(s) 73, 124, 181, 249, 403, 519
TfiI GAWTC 1 cut(s) 745
Tru1I TTAA 3 cut(s) 345, 522, 725
Tru9I TTAA 3 cut(s) 345, 522, 725
TscAI CASTG 3 cut(s) 470, 675, 771
TseFI GTSAC 2 cut(s) 197, 624
TseI GCWGC 1 cut(s) 665
Tsp45I GTSAC 2 cut(s) 197, 624
TspDTI ATGAA 2 cut(s) 257, 557
TspRI CASTG 3 cut(s) 470, 675, 771
Vha464I CTTAAG 1 cut(s) 724
VpaK11BI GGWCC 3 cut(s) 362, 419, 785
VspI ATTAAT 1 cut(s) 522
XapI RAATTY 5 cut(s) 73, 124, 181, 249, 403
XmaJI CCTAGG 1 cut(s) 107
XmiI GTMKAC 1 cut(s) 690
XspI CTAG 5 cut(s) 108, 143, 161, 533, 758
ZraI GACGTC 1 cut(s) 302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.