Rw4G014740

gene silencing by RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0001623
Physical Location & Seq
Reverse (-)
34148557 .. 34151231
2675 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G014740.1

Sequence Viewer

Length: 2592 bp
ATGTCGACGAAGGCGTTAATTGTAGCAGGAGAAAAACCTCTTGAACAAATTAACAACGCATCTACTCGTATACGTGTTCCCATGAAGAGGTCTGGCATTGGATCTAGAGGTAACAGGATTCCACTTCTTACTAACCATTTCAAGGTGGGAGTGAACAAATATACAGATGACTTTTACCACTATAGTATTGCAATGCAATATGATGACGGAACTCCAGTTGAAGGAAAAGGCATTGGATGCAAAGTTCTTGATAAAATTAAAGAGATATACGGCATGGAGCTGGCAAACAAAGAATTTGCGTATGATGGACAGAAGAGCTTGTTTACTGTTGGTCAACTCCCTCATAAAAGGCTTGATTTCATGATCGTATTGGATGACATGTCATTGAGCAGGTCTGGGGGATCCAGACGCGGTTACTTTTTAGAAGAGAGGGAGGGTGATAGGAAGAGAGTAAAGCAGTCATTTGGTTCCAAAACAATAAAGGTGCAGTTCAACTTTGCTGCTATAATCCCAATGCAAGCAATTGTGAATGCCTTACGAGGTCAGGACTCTGACCATTTTCAGGAAGCAGTTAGAGTTCTTGACATCATTCTTAGGCAGAATGCAGCAAAGCAGGGTTGTCTCCTTGTTCGGCAGTCATTCTTCCATAACAAGCCGAGGAGCTTTATAGAGCTGGGAGGAGGGGTGTTAGGCTGTAGGGGTTTACATTCAAGCTTCCGAGCCACCCAAGGTGGCCTATGTCTGAATATGGATGTATCCGCTACAATGATTCTAAAACCTGGTCCTGTTGTGAATTTCCTCATTGAGAACCAAAATGTCAAAACTCCTTACCAGATTGATTGGACTAAGGAGTACAAAATCACTGGATTGAGTGATAAACCCTGCAGACAGCAGACCTTCTTTTTGAAATCAAAAAAAGGACAGAATGAAGATGGAGTAGAGATCACAGTGTGTGAATACTTTGCTCAGTATAAGCAGATGCAGTTACGCGATTCAGCAGATTTTCCATGCATTAATGTTGGAAAGCCAAAGAAACCATCCTACTTTCCTCTTGAGCTTTGTGACTTGGTTTCTTTGCAACGCTATACCAAGTCATTGTCCAATATCCAAAGGGCTTCACTAGTGGAGAAGTCTAGGCAGAAGCCCCAGGAGCGAATGTCAACTTTGCGTGATGCACTGAGGAGTAGCAAGTATGAGGCTGACGTGATGCTTCGTTCTGCTGGAATATCGATTGGTACAGAACTCATGCAGGTTGAAGGTCGTGTGTTGTCAGCCCCAAATTTGAAAGTGGGAAATGGAGATGTCATTTTTCCTAGGAATGGGAGATGGAATTTCAACAATAAAAAACTAGTAGATCCTGTAAAACTAGAACGCTGGGCTGTTGTGAATTTCTCTGCTCGCTGTGACATTCGGTACCTGGTTGACACTATGATAAAGTGTGGAGATATGAAAGGAATTTCCATCAAGCCTCCATTTGGTGTGTTTGAAGAGAGTATTCAGAACAGACGTCAGTCAGCTCCGGTTAGAGTAGAAAAGATGTTTGAATACATTAAGCCCAAACTTCCAGGACCACCACAGTTTTTGTTGTGCATTCTCCCAGAGAGGAAGAATTCTGAGATATATGGTCCATGGAAAAGGAAAAACCTTTCAGACATTGGGATTGCAACGCAGTGCATTGCACCTACGAAGGTGAACGATCAATACATCACTAACGTGCTCCTGAAAATTAATGCAAAGCTAGGTGGAATGAACTCCTTGCTAACTATGGAGCATTCTCCTTCTCTACCCTTGGTTTCCAAGTCCCCTACTCTGATATTAGGGATGGATGTGTCACATGGCTCACCTGGGCAGTCAAATATGCCTTCTATTGCAGCAGTGGTCAGTTCTAGGCACTGGCCTTGGATTTCTCGTTACCGAGCTGCAGTTCGTACTCAGTCACCAAAAGTAGAAATGATTGCCAATCTGTTCAAGCCTGAACTTTTGCTAGACTTTTATAATACTTCAAACTACAGGAAGCCGGAGCAGATTATTATCTTCAGGGATGGAGTGAGTGAATCACAGTTCAACCAAGTCTTGAATCTGGAATTGGATCAGATTATTAAGGCCTGCAAGTTCATTGACGAGAGCTGGTTGCCAAAGTTCATGGTTATTATTGCACAAAAGCGACATCACACAAAATTCTTTCAGGCTGGTGGTTCTTCTGACAATGTTCCACCTGGGACTATCGTTGACAACAAAGTCTGTGATCCAAGAAACAATGATTTCTACTTGTGTTCTCATGCTGGGATGATTGGAACAAGTCGGCCTACTCATTATCATGTTCTATATGATGAACTTGGCTTTTCAGCAGATGACTTGCAAGATCTAGTTCATTCTTTGTCGTATGTTTATCAAAGGAGCACGACGGCCGTATCTCTAGTTGCTCCAATATGCTATGCCCATCTAGCAGCAGCACAGATGAGTCAGTTCATTAGGTTTGATGAAATGTCAGAGACTGCCTCAAGCCAAGGTAGTGTTACTGTTGCTGGAGATGCCAGCAAATGGCCAGGTCAGGTCATCATGGGTCTGGCCCAGTCCTACCCTAAATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

863

Amino Acids

97.06

Weight (kDa)

9.36

Isoelectric Point (pI)

42.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArgoN PF16486 39 - 198 3.2e-23 N-terminal domain of argonaute
ArgoL1 PF08699 210 - 258 7.6e-16 Argonaute linker 1 domain
PAZ PF02170 269 - 374 3.2e-21 PAZ domain
ArgoL2 PF16488 384 - 429 1.6e-09 Argonaute linker 2 domain
ArgoMid PF16487 438 - 489 1e-05 Mid domain of argonaute
Piwi PF02171 528 - 824 1.3e-103 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1995
AasI GACNNNNNNGTC 1 cut(s) 2237
AatII GACGTC 1 cut(s) 1510
Acc36I ACCTGC 2 cut(s) 381, 1240
Acc65I GGTACC 1 cut(s) 1413
AccB1I GGYRCC 1 cut(s) 1413
AccB7I CCANNNNNTGG 1 cut(s) 2541
AccI GTMKAC 2 cut(s) 5, 70
AccII CGCG 2 cut(s) 411, 990
AciI CCGC 2 cut(s) 411, 759
AclWI GGATC 6 cut(s) 109, 396, 409, 1349, 2097, 2240
AcoI YGGCCR 2 cut(s) 2406, 2543
AcsI RAATTY 9 cut(s) 293, 793, 1279, 1330, 1387, 1455, 1609, 2177, 2585
AcuI CTGAAG 1 cut(s) 2020
AcyI GRCGYC 1 cut(s) 1507
AdeI CACNNNGTG 1 cut(s) 951
AfaI GTAC 4 cut(s) 854, 1237, 1415, 1930
AfiI CCNNNNNNNGG 7 cut(s) 87, 142, 221, 562, 1319, 1475, 2541
AflIII ACRYGT 2 cut(s) 73, 378
AhlI ACTAGT 2 cut(s) 1120, 1348
AjiI CACGTC 1 cut(s) 1204
AjnI CCWGG 7 cut(s) 778, 1146, 1416, 1564, 1843, 2215, 2545
AjuI GAANNNNNNNTTGG 2 cut(s) 2069, 2101
AleI CACNNNNGTG 1 cut(s) 1712
AloI GAACNNNNNNTCC 2 cut(s) 228, 260
Alw21I GWGCWC 2 cut(s) 1719, 2402
Alw26I GTCTC 2 cut(s) 626, 2486
AlwI GGATC 6 cut(s) 109, 396, 409, 1349, 2097, 2240
AlwNI CAGNNNCTG 1 cut(s) 2495
AoxI GGCC 7 cut(s) 733, 1895, 2103, 2303, 2406, 2543, 2568
ApeKI GCWGC 6 cut(s) 500, 605, 1871, 1919, 2447, 2450
ApoI RAATTY 9 cut(s) 293, 793, 1279, 1330, 1387, 1455, 1609, 2177, 2585
ArsI GACNNNNNNTTYG 2 cut(s) 886, 918
AseI ATTAAT 2 cut(s) 1014, 1728
Asp718I GGTACC 1 cut(s) 1413
AspA2I CCTAGG 1 cut(s) 1313
AspS9I GGNCC 4 cut(s) 782, 1568, 1625, 2569
AsuHPI GGTGA 4 cut(s) 449, 1702, 1833, 1929
AvaII GGWCC 3 cut(s) 782, 1568, 1625
AvrII CCTAGG 1 cut(s) 1313
BaeI ACNNNNGTAYC 2 cut(s) 2394, 2427
BalI TGGCCA 1 cut(s) 2545
BamHI GGATCC 1 cut(s) 401
BanI GGYRCC 1 cut(s) 1413
Bbv12I GWGCWC 2 cut(s) 1719, 2402
BbvI GCAGC 6 cut(s) 487, 617, 1883, 1906, 2459, 2462
BccI CCATC 8 cut(s) 299, 926, 1045, 1320, 1469, 1816, 2036, 2448
BceAI ACGGC 3 cut(s) 286, 2393, 2421
BciT130I CCWGG 7 cut(s) 780, 1148, 1418, 1566, 1845, 2217, 2547
BciVI GTATCC 1 cut(s) 766
BcoDI GTCTC 2 cut(s) 626, 2486
BcuI ACTAGT 2 cut(s) 1120, 1348
BfmI CTRYAG 5 cut(s) 181, 694, 883, 1920, 2008
BfuAI ACCTGC 2 cut(s) 381, 1240
BfuI GTATCC 1 cut(s) 766
BglII AGATCT 1 cut(s) 2362
BisI GCNGC 6 cut(s) 501, 606, 1872, 1920, 2448, 2451
BlnI CCTAGG 1 cut(s) 1313
BlsI GCNGC 6 cut(s) 502, 607, 1873, 1921, 2449, 2452
Bme1390I CCNGG 7 cut(s) 780, 1148, 1418, 1566, 1845, 2217, 2547
Bme18I GGWCC 3 cut(s) 782, 1568, 1625
BmgBI CACGTC 1 cut(s) 1204
BmgT120I GGNCC 4 cut(s) 782, 1568, 1625, 2569
BmiI GGNNCC 3 cut(s) 403, 469, 1415
BmrFI CCNGG 7 cut(s) 780, 1148, 1418, 1566, 1845, 2217, 2547
BmrI ACTGGG 1 cut(s) 2566
BmsI GCATC 6 cut(s) 68, 227, 969, 1162, 1197, 2521
BmuI ACTGGG 1 cut(s) 2566
BoxI GACNNNNGTC 1 cut(s) 1510
BplI GAGNNNNNCTC 2 cut(s) 2483, 2515
BpmI CTGGAG 2 cut(s) 198, 2547
BpuEI CTTGAG 2 cut(s) 1073, 2485
Bsa29I ATCGAT 1 cut(s) 1229
BsaAI YACGTR 1 cut(s) 74
BsaBI GATNNNNATC 1 cut(s) 2030
BsaHI GRCGYC 1 cut(s) 1507
BsaWI WCCGGW 1 cut(s) 1519
BsaXI ACNNNNNCTCC 4 cut(s) 669, 699, 1142, 1172
Bsc4I CCNNNNNNNGG 7 cut(s) 87, 142, 221, 562, 1319, 1475, 2541
Bse1I ACTGG 4 cut(s) 215, 868, 1898, 2572
Bse3DI GCAATG 2 cut(s) 198, 1674
Bse8I GATNNNNATC 1 cut(s) 2030
BseBI CCWGG 7 cut(s) 780, 1148, 1418, 1566, 1845, 2217, 2547
BseCI ATCGAT 1 cut(s) 1229
BseGI GGATG 8 cut(s) 242, 379, 757, 1037, 1827, 1831, 2047, 2292
BseJI GATNNNNATC 1 cut(s) 2030
BseLI CCNNNNNNNGG 7 cut(s) 87, 142, 221, 562, 1319, 1475, 2541
BseMI GCAATG 2 cut(s) 198, 1674
BseMII CTCAG 4 cut(s) 980, 1169, 1605, 1946
BseNI ACTGG 4 cut(s) 215, 868, 1898, 2572
BseRI GAGGAG 3 cut(s) 673, 693, 1195
BseX3I CGGCCG 1 cut(s) 2406
BseXI GCAGC 6 cut(s) 487, 617, 1883, 1906, 2459, 2462
BseYI CCCAGC 3 cut(s) 673, 1374, 2282
BsgI GTGCAG 1 cut(s) 506
Bsh1236I CGCG 2 cut(s) 411, 990
Bsh1285I CGRYCG 1 cut(s) 2409
BshFI GGCC 7 cut(s) 735, 1897, 2105, 2305, 2408, 2545, 2570
BshNI GGYRCC 1 cut(s) 1413
BshVI ATCGAT 1 cut(s) 1229
BsiEI CGRYCG 1 cut(s) 2409
BsiHKAI GWGCWC 2 cut(s) 1719, 2402
BsiSI CCGG 2 cut(s) 1520, 2018
BslFI GGGAC 2 cut(s) 1786, 2233
BslI CCNNNNNNNGG 7 cut(s) 87, 142, 221, 562, 1319, 1475, 2541
BsmAI GTCTC 2 cut(s) 626, 2486
BsmFI GGGAC 2 cut(s) 1786, 2233
BsmI GAATGC 4 cut(s) 535, 607, 1590, 1771
BsnI GGCC 7 cut(s) 735, 1897, 2105, 2305, 2408, 2545, 2570
Bsp1286I GDGCHC 2 cut(s) 1719, 2402
Bsp143I GATC 9 cut(s) 101, 363, 401, 942, 1354, 1696, 2089, 2245, 2362
Bsp19I CCATGG 1 cut(s) 1628
BspACI CCGC 2 cut(s) 411, 759
BspANI GGCC 7 cut(s) 735, 1897, 2105, 2305, 2408, 2545, 2570
BspCNI CTCAG 4 cut(s) 979, 1170, 1606, 1945
BspDI ATCGAT 1 cut(s) 1229
BspFNI CGCG 2 cut(s) 411, 990
BspHI TCATGA 1 cut(s) 360
BspLI GGNNCC 3 cut(s) 403, 469, 1415
BspMAI CTGCAG 2 cut(s) 887, 1924
BspMI ACCTGC 2 cut(s) 381, 1240
BspPI GGATC 6 cut(s) 109, 396, 409, 1349, 2097, 2240
BspQI GCTCTTC 1 cut(s) 308
BspT107I GGYRCC 1 cut(s) 1413
BsrDI GCAATG 2 cut(s) 198, 1674
BsrI ACTGG 4 cut(s) 215, 868, 1898, 2572
BssMI GATC 9 cut(s) 101, 363, 401, 942, 1354, 1696, 2089, 2245, 2362
BssNAI GTATAC 1 cut(s) 71
BssNI GRCGYC 1 cut(s) 1507
BssT1I CCWWGG 6 cut(s) 727, 1313, 1628, 1788, 1898, 2506
Bst1107I GTATAC 1 cut(s) 71
Bst2UI CCWGG 7 cut(s) 780, 1148, 1418, 1566, 1845, 2217, 2547
Bst4CI ACNGT 5 cut(s) 328, 949, 1578, 2061, 2521
Bst6I CTCTTC 5 cut(s) 80, 308, 420, 440, 1482
BstACI GRCGYC 1 cut(s) 1507
BstAPI GCANNNNNTGC 1 cut(s) 237
BstBAI YACGTR 1 cut(s) 74
BstC8I GCNNGC 5 cut(s) 282, 519, 1399, 2107, 2536
BstDEI CTNAG 6 cut(s) 593, 846, 966, 1178, 1614, 1932
BstDSI CCRYGG 1 cut(s) 1628
BstF5I GGATG 8 cut(s) 242, 379, 757, 1037, 1827, 1831, 2047, 2292
BstFNI CGCG 2 cut(s) 411, 990
BstKTI GATC 9 cut(s) 104, 366, 404, 945, 1357, 1699, 2092, 2248, 2365
BstMAI GTCTC 2 cut(s) 626, 2486
BstMBI GATC 9 cut(s) 101, 363, 401, 942, 1354, 1696, 2089, 2245, 2362
BstMCI CGRYCG 1 cut(s) 2409
BstMWI GCNNNNNNNGC 4 cut(s) 237, 1150, 2444, 2531
BstNI CCWGG 7 cut(s) 780, 1148, 1418, 1566, 1845, 2217, 2547
BstNSI RCATGY 1 cut(s) 382
BstPAI GACNNNNGTC 1 cut(s) 1510
BstSCI CCNGG 7 cut(s) 778, 1146, 1416, 1564, 1843, 2215, 2545
BstSFI CTRYAG 5 cut(s) 181, 694, 883, 1920, 2008
BstUI CGCG 2 cut(s) 411, 990
BstV1I GCAGC 6 cut(s) 487, 617, 1883, 1906, 2459, 2462
BstX2I RGATCY 4 cut(s) 101, 401, 1354, 2362
BstYI RGATCY 4 cut(s) 101, 401, 1354, 2362
BstZ17I GTATAC 1 cut(s) 71
BstZI CGGCCG 1 cut(s) 2406
Bsu15I ATCGAT 1 cut(s) 1229
BsuI GTATCC 1 cut(s) 766
BsuRI GGCC 7 cut(s) 735, 1897, 2105, 2305, 2408, 2545, 2570
BsuTUI ATCGAT 1 cut(s) 1229
BtgI CCRYGG 1 cut(s) 1628
BtrI CACGTC 1 cut(s) 1204
BtsCI GGATG 8 cut(s) 242, 379, 757, 1037, 1827, 1831, 2047, 2292
BtsI GCAGTG 2 cut(s) 1676, 1881
BtsIMutI CAGTG 6 cut(s) 861, 954, 1175, 1676, 1881, 1891
BveI ACCTGC 2 cut(s) 381, 1240
Cac8I GCNNGC 5 cut(s) 282, 519, 1399, 2107, 2536
CaiI CAGNNNCTG 1 cut(s) 2495
CciI TCATGA 1 cut(s) 360
Cfr13I GGNCC 4 cut(s) 782, 1568, 1625, 2569
ClaI ATCGAT 1 cut(s) 1229
CseI GACGC 1 cut(s) 417
CsiI ACCWGGT 2 cut(s) 778, 1416
Csp6I GTAC 4 cut(s) 853, 1236, 1414, 1929
CspCI CAANNNNNGTGG 2 cut(s) 1563, 1598
CviQI GTAC 4 cut(s) 853, 1236, 1414, 1929
DdeI CTNAG 6 cut(s) 593, 846, 966, 1178, 1614, 1932
DpnI GATC 9 cut(s) 103, 365, 403, 944, 1356, 1698, 2091, 2247, 2364
DpnII GATC 9 cut(s) 101, 363, 401, 942, 1354, 1696, 2089, 2245, 2362
DraIII CACNNNGTG 1 cut(s) 951
DrdI GACNNNNNNGTC 1 cut(s) 2237
DseDI GACNNNNNNGTC 1 cut(s) 2237
EaeI YGGCCR 2 cut(s) 2406, 2543
EagI CGGCCG 1 cut(s) 2406
Eam1104I CTCTTC 5 cut(s) 80, 308, 420, 440, 1482
EarI CTCTTC 5 cut(s) 80, 308, 420, 440, 1482
EclXI CGGCCG 1 cut(s) 2406
Eco130I CCWWGG 6 cut(s) 727, 1313, 1628, 1788, 1898, 2506
Eco147I AGGCCT 1 cut(s) 2105
Eco47I GGWCC 3 cut(s) 782, 1568, 1625
Eco52I CGGCCG 1 cut(s) 2406
Eco57I CTGAAG 1 cut(s) 2020
EcoRI GAATTC 1 cut(s) 1609
EcoRII CCWGG 7 cut(s) 778, 1146, 1416, 1564, 1843, 2215, 2545
EcoT14I CCWWGG 6 cut(s) 727, 1313, 1628, 1788, 1898, 2506
EcoT22I ATGCAT 1 cut(s) 1013
ErhI CCWWGG 6 cut(s) 727, 1313, 1628, 1788, 1898, 2506
FaqI GGGAC 2 cut(s) 1786, 2233
FblI GTMKAC 2 cut(s) 5, 70
Fnu4HI GCNGC 6 cut(s) 501, 606, 1872, 1920, 2448, 2451
FokI GGATG 8 cut(s) 249, 386, 764, 1024, 1834, 1838, 2054, 2299
Fsp4HI GCNGC 6 cut(s) 501, 606, 1872, 1920, 2448, 2451
GluI GCNGC 6 cut(s) 501, 606, 1872, 1920, 2448, 2451
GsaI CCCAGC 3 cut(s) 677, 1378, 2286
GsuI CTGGAG 2 cut(s) 198, 2547
HaeIII GGCC 7 cut(s) 735, 1897, 2105, 2305, 2408, 2545, 2570
HapII CCGG 2 cut(s) 1520, 2018
HgaI GACGC 1 cut(s) 417
Hin1I GRCGYC 1 cut(s) 1507
HincII GTYRAC 5 cut(s) 6, 335, 1161, 1423, 2230
HindII GTYRAC 5 cut(s) 6, 335, 1161, 1423, 2230
HindIII AAGCTT 1 cut(s) 712
HinfI GANTC 7 cut(s) 118, 548, 769, 992, 2054, 2077, 2461
HpaII CCGG 2 cut(s) 1520, 2018
HphI GGTGA 4 cut(s) 449, 1702, 1833, 1929
Hpy99I CGWCG 2 cut(s) 10, 2407
HpyAV CCTTC 7 cut(s) 4, 215, 907, 1250, 1681, 1788, 1872
HpyCH4III ACNGT 5 cut(s) 328, 949, 1578, 2061, 2521
HpyCH4IV ACGT 4 cut(s) 73, 1203, 1507, 1713
HpyF10VI GCNNNNNNNGC 4 cut(s) 237, 1150, 2444, 2531
HpyF3I CTNAG 6 cut(s) 593, 846, 966, 1178, 1614, 1932
HpySE526I ACGT 4 cut(s) 73, 1203, 1507, 1713
Hsp92I GRCGYC 1 cut(s) 1507
KpnI GGTACC 1 cut(s) 1417
Kzo9I GATC 9 cut(s) 101, 363, 401, 942, 1354, 1696, 2089, 2245, 2362
LguI GCTCTTC 1 cut(s) 308
LmnI GCTCC 9 cut(s) 277, 660, 1150, 1522, 1722, 1768, 2020, 2397, 2428
Lsp1109I GCAGC 6 cut(s) 487, 617, 1883, 1906, 2459, 2462
LweI GCATC 6 cut(s) 68, 227, 969, 1162, 1197, 2521
MabI ACCWGGT 2 cut(s) 778, 1416
MaeII ACGT 4 cut(s) 73, 1203, 1507, 1713
MaeIII GTNAC 9 cut(s) 110, 413, 984, 1061, 1403, 1830, 1910, 1935, 2515
MalI GATC 9 cut(s) 103, 365, 403, 944, 1356, 1698, 2091, 2247, 2364
MboI GATC 9 cut(s) 101, 363, 401, 942, 1354, 1696, 2089, 2245, 2362
MfeI CAATTG 1 cut(s) 522
MflI RGATCY 4 cut(s) 101, 401, 1354, 2362
MhlI GDGCHC 2 cut(s) 1719, 2402
MlsI TGGCCA 1 cut(s) 2545
MluNI TGGCCA 1 cut(s) 2545
MlyI GAGTC 2 cut(s) 542, 2470
MmeI TCCRAC 1 cut(s) 1000
Mox20I TGGCCA 1 cut(s) 2545
Mph1103I ATGCAT 1 cut(s) 1013
MscI TGGCCA 1 cut(s) 2545
MseI TTAA 7 cut(s) 17, 51, 258, 1014, 1551, 1728, 2100
MslI CAYNNNNRTG 2 cut(s) 1712, 2316
Msp20I TGGCCA 1 cut(s) 2545
MspI CCGG 2 cut(s) 1520, 2018
MspR9I CCNGG 7 cut(s) 780, 1148, 1418, 1566, 1845, 2217, 2547
MunI CAATTG 1 cut(s) 522
Mva1269I GAATGC 4 cut(s) 535, 607, 1590, 1771
MvaI CCWGG 7 cut(s) 780, 1148, 1418, 1566, 1845, 2217, 2547
MvnI CGCG 2 cut(s) 411, 990
MwoI GCNNNNNNNGC 4 cut(s) 237, 1150, 2444, 2531
NcoI CCATGG 1 cut(s) 1628
NdeII GATC 9 cut(s) 101, 363, 401, 942, 1354, 1696, 2089, 2245, 2362
NlaIV GGNNCC 3 cut(s) 403, 469, 1415
NmeAIII GCCGAG 1 cut(s) 681
NmuCI GTSAC 4 cut(s) 1061, 1403, 1830, 1935
NsiI ATGCAT 1 cut(s) 1013
NspI RCATGY 1 cut(s) 382
OliI CACNNNNGTG 1 cut(s) 1712
PagI TCATGA 1 cut(s) 360
PceI AGGCCT 1 cut(s) 2105
PciI ACATGT 1 cut(s) 378
PciSI GCTCTTC 1 cut(s) 308
PcsI WCGNNNNNNNCGW 1 cut(s) 11
PctI GAATGC 4 cut(s) 535, 607, 1590, 1771
PfeI GAWTC 5 cut(s) 118, 769, 992, 2054, 2077
PflMI CCANNNNNTGG 1 cut(s) 2541
PfoI TCCNGGA 1 cut(s) 1564
PkrI GCNGC 6 cut(s) 502, 607, 1873, 1921, 2449, 2452
PleI GAGTC 2 cut(s) 542, 2469
PpsI GAGTC 2 cut(s) 542, 2469
Ppu21I YACGTR 1 cut(s) 74
PscI ACATGT 1 cut(s) 378
PshAI GACNNNNGTC 1 cut(s) 1510
PshBI ATTAAT 2 cut(s) 1014, 1728
PsiI TTATAA 1 cut(s) 1995
Psp6I CCWGG 7 cut(s) 778, 1146, 1416, 1564, 1843, 2215, 2545
PspFI CCCAGC 3 cut(s) 673, 1374, 2282
PspGI CCWGG 7 cut(s) 778, 1146, 1416, 1564, 1843, 2215, 2545
PspN4I GGNNCC 3 cut(s) 403, 469, 1415
PspPI GGNCC 4 cut(s) 782, 1568, 1625, 2569
PsrI GAACNNNNNNTAC 2 cut(s) 1685, 1717
PstI CTGCAG 2 cut(s) 887, 1924
PstNI CAGNNNCTG 1 cut(s) 2495
PsuI RGATCY 4 cut(s) 101, 401, 1354, 2362
RsaI GTAC 4 cut(s) 854, 1237, 1415, 1930
RsaNI GTAC 4 cut(s) 853, 1236, 1414, 1929
RseI CAYNNNNRTG 2 cut(s) 1712, 2316
SalI GTCGAC 1 cut(s) 4
SapI GCTCTTC 1 cut(s) 308
SaqAI TTAA 7 cut(s) 17, 51, 258, 1014, 1551, 1728, 2100
SatI GCNGC 6 cut(s) 501, 606, 1872, 1920, 2448, 2451
Sau3AI GATC 9 cut(s) 101, 363, 401, 942, 1354, 1696, 2089, 2245, 2362
Sau96I GGNCC 4 cut(s) 782, 1568, 1625, 2569
SchI GAGTC 2 cut(s) 542, 2470
ScrFI CCNGG 7 cut(s) 780, 1148, 1418, 1566, 1845, 2217, 2547
SduI GDGCHC 2 cut(s) 1719, 2402
SexAI ACCWGGT 2 cut(s) 778, 1416
SfaNI GCATC 6 cut(s) 68, 227, 969, 1162, 1197, 2521
SfcI CTRYAG 5 cut(s) 181, 694, 883, 1920, 2008
SinI GGWCC 3 cut(s) 782, 1568, 1625
SmiMI CAYNNNNRTG 2 cut(s) 1712, 2316
SmlI CTYRAG 2 cut(s) 1052, 2500
SmoI CTYRAG 2 cut(s) 1052, 2500
SpeI ACTAGT 2 cut(s) 1120, 1348
SseBI AGGCCT 1 cut(s) 2105
SsiI CCGC 2 cut(s) 411, 759
StuI AGGCCT 1 cut(s) 2105
StyD4I CCNGG 7 cut(s) 778, 1146, 1416, 1564, 1843, 2215, 2545
StyI CCWWGG 6 cut(s) 727, 1313, 1628, 1788, 1898, 2506
TaaI ACNGT 5 cut(s) 328, 949, 1578, 2061, 2521
TaiI ACGT 4 cut(s) 76, 1206, 1510, 1716
TaqI TCGA 2 cut(s) 5, 1229
TatI WGTACW 1 cut(s) 852
TfiI GAWTC 5 cut(s) 118, 769, 992, 2054, 2077
Tru1I TTAA 7 cut(s) 17, 51, 258, 1014, 1551, 1728, 2100
Tru9I TTAA 7 cut(s) 17, 51, 258, 1014, 1551, 1728, 2100
TscAI CASTG 6 cut(s) 868, 954, 1182, 1676, 1881, 1898
TseFI GTSAC 4 cut(s) 1061, 1403, 1830, 1935
TseI GCWGC 6 cut(s) 500, 605, 1871, 1919, 2447, 2450
Tsp45I GTSAC 4 cut(s) 1061, 1403, 1830, 1935
TspGWI ACGGA 1 cut(s) 222
TspRI CASTG 6 cut(s) 868, 954, 1182, 1676, 1881, 1898
Van91I CCANNNNNTGG 1 cut(s) 2541
VpaK11BI GGWCC 3 cut(s) 782, 1568, 1625
VspI ATTAAT 2 cut(s) 1014, 1728
XapI RAATTY 9 cut(s) 293, 793, 1279, 1330, 1387, 1455, 1609, 2177, 2585
XbaI TCTAGA 1 cut(s) 104
XceI RCATGY 1 cut(s) 382
XmaJI CCTAGG 1 cut(s) 1313
XmiI GTMKAC 2 cut(s) 5, 70
ZraI GACGTC 1 cut(s) 1508
Zsp2I ATGCAT 1 cut(s) 1013
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.