RchiOBHm_Chr7g0206231

Argonaute

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001623
Physical Location & Seq
Reverse (-)
23964227 .. 23966379
2153 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ18456

Sequence Viewer

Length: 1146 bp
ATGCAATATGATGATGGAACCGAAGTTGACGGGAAAGGCATTGGACGTAAGGTTCTTGAAAAAGTAAAAGACACATATAGTGTGGAGCTGGAAAACAAGGATTTTGCTTATGATGGAGAGAAGAGCTTGTTTACTGTTGGGCAGCTCCCTCATAAAAAACTTGATTTCACAGTAGTACTGGATGACATATCATCAAGCAGTGGTTATTTTGCACACAGGTCTAGGACGAATGGGGGATCTGGGGGCAGTTACTCCCTAGAAGAGAGTCAGGGCGATAGGAAGAGAGTCAAGCAGTCATTTCAATCCAAAACAATAAAGGTACAGGTCAACTTTGCTGCTAAAATCCCAATGCAAGCAATCACGAATGCCTTGCGAGGTCAGGACTCTGACCATTTTCAGGAAGCAATTAGAGTTCTTGACATCATTATAAGGCAGAATGCAGCAAAGCAGGGTTGTCTCCTTGTTCGGGAATCTTTCTTCCATAACAACCCGAGGAACTTCATAGAATTAGGAGGAGGGGTGTTAGGCTGTAGGGGTTTTCATTCAAGCTTCCGGGCCACGCAAGGCGGCCTATCCCTGAATATGGGACACGATGGAGATGGAGAAGAGATCACTGTTTATGAATACTTTGCCCAGCATAAGCACATGCAGTTACACGATTCAGCAGATTTTCCCTACATCAATGTTGGAAAACCAAAGAAACCTTCTTACTTTCCCCTAGAGCTTTGTAATTTGGTTTCCTTGCAACGCTATACCAAGGCTTTATCCAATCTCCAAAGGGCTTCACTAGTGGAGAAGTCTCGGCAGAAACCCCAGGAGCGAATGTCAACTTTGCGTGATCTTGGCGAAATCAACTCCTTGCTAATGGTGGAGCATTCTCCTTCTCTGCCCTTGGTATCCAAGTCCCCCACTCTGATTTTAGGGATGGATGTGTCACATGGCTCACCTGGGCGTTCCGATGTGCCTTCTATTGCAGCGGTGGTCAGTTCCAGGAAATGGCCTTTGATTTCTCGTTACCGAGCTGCAGTTCGTACTCAATCACCAAAAGTAGAAATGATTGCTAATCTGTTCAAACCTGTGTCTGATAAAGAGGACCAAGGCATAATCAAGTTGGCTCCTATCTCCCCCTATACTCTGCCACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

381

Amino Acids

42.35

Weight (kDa)

9.1

Isoelectric Point (pI)

39.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArgoN PF16486 2 - 143 7.4e-20 N-terminal domain of argonaute
ArgoL1 PF08699 155 - 195 4.8e-13 Argonaute linker 1 domain
PAZ PF02170 199 - 263 7e-15 PAZ domain
Piwi PF02171 281 - 357 9.3e-13 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 428
AccB7I CCANNNNNTGG 1 cut(s) 996
AciI CCGC 2 cut(s) 567, 977
AclWI GGATC 1 cut(s) 244
AfaI GTAC 3 cut(s) 177, 321, 1033
AfiI CCNNNNNNNGG 4 cut(s) 397, 466, 583, 996
AgsI TTSAA 4 cut(s) 59, 302, 546, 1072
AhlI ACTAGT 1 cut(s) 787
AjnI CCWGG 3 cut(s) 813, 946, 989
AjuI GAANNNNNNNTTGG 2 cut(s) 688, 720
AloI GAACNNNNNNTCC 2 cut(s) 36, 68
AluBI AGCT 6 cut(s) 88, 126, 145, 549, 724, 1022
AluI AGCT 6 cut(s) 88, 126, 145, 549, 724, 1022
Alw26I GTCTC 2 cut(s) 461, 804
AlwI GGATC 1 cut(s) 244
Ama87I CYCGRG 1 cut(s) 490
AoxI GGCC 3 cut(s) 555, 568, 998
ApeKI GCWGC 5 cut(s) 142, 335, 440, 974, 1022
AspS9I GGNCC 2 cut(s) 555, 1093
AsuC2I CCSGG 1 cut(s) 554
AsuHPI GGTGA 2 cut(s) 936, 1032
AvaI CYCGRG 1 cut(s) 490
AvaII GGWCC 1 cut(s) 1093
BbvI GCAGC 5 cut(s) 154, 322, 452, 986, 1009
BccI CCATC 5 cut(s) 8, 107, 587, 593, 919
BcgI CGANNNNNNTGC 2 cut(s) 352, 386
BciT130I CCWGG 3 cut(s) 815, 948, 991
BciVI GTATCC 1 cut(s) 907
BcnI CCSGG 1 cut(s) 554
BcoDI GTCTC 2 cut(s) 461, 804
BcuI ACTAGT 1 cut(s) 787
BfaI CTAG 4 cut(s) 222, 257, 719, 788
BfmI CTRYAG 2 cut(s) 529, 1023
BfuI GTATCC 1 cut(s) 907
BisI GCNGC 6 cut(s) 143, 336, 441, 568, 975, 1023
BlsI GCNGC 6 cut(s) 144, 337, 442, 569, 976, 1024
BmcAI AGTACT 1 cut(s) 177
Bme1390I CCNGG 4 cut(s) 554, 815, 948, 991
Bme18I GGWCC 1 cut(s) 1093
BmeT110I CYCGRG 1 cut(s) 490
BmgT120I GGNCC 2 cut(s) 555, 1093
BmiI GGNNCC 2 cut(s) 19, 1116
BmrFI CCNGG 4 cut(s) 554, 815, 948, 991
BpuMI CCSGG 1 cut(s) 554
BsaJI CCNNGG 6 cut(s) 491, 756, 813, 891, 947, 1096
BsaXI ACNNNNNCTCC 4 cut(s) 504, 534, 809, 839
Bsc4I CCNNNNNNNGG 4 cut(s) 397, 466, 583, 996
Bse1I ACTGG 1 cut(s) 183
BseBI CCWGG 3 cut(s) 815, 948, 991
BseDI CCNNGG 6 cut(s) 491, 756, 813, 891, 947, 1096
BseGI GGATG 3 cut(s) 187, 930, 934
BseLI CCNNNNNNNGG 4 cut(s) 397, 466, 583, 996
BseNI ACTGG 1 cut(s) 183
BseRI GAGGAG 1 cut(s) 528
BseXI GCAGC 5 cut(s) 154, 322, 452, 986, 1009
BseYI CCCAGC 1 cut(s) 633
BshFI GGCC 3 cut(s) 557, 570, 1000
BsiHKCI CYCGRG 1 cut(s) 490
BsiSI CCGG 1 cut(s) 553
BslFI GGGAC 2 cut(s) 600, 889
BslI CCNNNNNNNGG 4 cut(s) 397, 466, 583, 996
BsmAI GTCTC 2 cut(s) 461, 804
BsmFI GGGAC 2 cut(s) 600, 889
BsmI GAATGC 3 cut(s) 370, 442, 874
BsnI GGCC 3 cut(s) 557, 570, 1000
BsoBI CYCGRG 1 cut(s) 490
Bsp143I GATC 3 cut(s) 236, 609, 838
BspACI CCGC 2 cut(s) 567, 977
BspANI GGCC 3 cut(s) 557, 570, 1000
BspLI GGNNCC 2 cut(s) 19, 1116
BspMAI CTGCAG 1 cut(s) 1027
BspPI GGATC 1 cut(s) 244
BspQI GCTCTTC 1 cut(s) 116
BsrI ACTGG 1 cut(s) 183
BssECI CCNNGG 6 cut(s) 491, 756, 813, 891, 947, 1096
BssMI GATC 3 cut(s) 236, 609, 838
BssT1I CCWWGG 3 cut(s) 756, 891, 1096
Bst2UI CCWGG 3 cut(s) 815, 948, 991
Bst4CI ACNGT 3 cut(s) 136, 172, 616
Bst6I CTCTTC 4 cut(s) 116, 255, 275, 600
BstC8I GCNNGC 1 cut(s) 354
BstF5I GGATG 3 cut(s) 187, 930, 934
BstKTI GATC 3 cut(s) 239, 612, 841
BstMAI GTCTC 2 cut(s) 461, 804
BstMBI GATC 3 cut(s) 236, 609, 838
BstNI CCWGG 3 cut(s) 815, 948, 991
BstNSI RCATGY 1 cut(s) 649
BstSCI CCNGG 4 cut(s) 552, 813, 946, 989
BstSFI CTRYAG 2 cut(s) 529, 1023
BstV1I GCAGC 5 cut(s) 154, 322, 452, 986, 1009
BstX2I RGATCY 1 cut(s) 236
BstYI RGATCY 1 cut(s) 236
BsuI GTATCC 1 cut(s) 907
BsuRI GGCC 3 cut(s) 557, 570, 1000
BtsCI GGATG 3 cut(s) 187, 930, 934
BtsI GCAGTG 1 cut(s) 205
BtsIMutI CAGTG 2 cut(s) 205, 612
Cac8I GCNNGC 1 cut(s) 354
Cfr13I GGNCC 2 cut(s) 555, 1093
Csp6I GTAC 3 cut(s) 176, 320, 1032
CviAII CATG 2 cut(s) 646, 938
CviQI GTAC 3 cut(s) 176, 320, 1032
DpnI GATC 3 cut(s) 238, 611, 840
DpnII GATC 3 cut(s) 236, 609, 838
Eam1104I CTCTTC 4 cut(s) 116, 255, 275, 600
EarI CTCTTC 4 cut(s) 116, 255, 275, 600
Eco130I CCWWGG 3 cut(s) 756, 891, 1096
Eco47I GGWCC 1 cut(s) 1093
Eco88I CYCGRG 1 cut(s) 490
EcoRII CCWGG 3 cut(s) 813, 946, 989
EcoT14I CCWWGG 3 cut(s) 756, 891, 1096
ErhI CCWWGG 3 cut(s) 756, 891, 1096
FaeI CATG 2 cut(s) 649, 941
FaqI GGGAC 2 cut(s) 600, 889
FatI CATG 2 cut(s) 645, 937
Fnu4HI GCNGC 6 cut(s) 143, 336, 441, 568, 975, 1023
FokI GGATG 3 cut(s) 194, 937, 941
Fsp4HI GCNGC 6 cut(s) 143, 336, 441, 568, 975, 1023
FspBI CTAG 4 cut(s) 222, 257, 719, 788
GluI GCNGC 6 cut(s) 143, 336, 441, 568, 975, 1023
GsaI CCCAGC 1 cut(s) 637
HaeIII GGCC 3 cut(s) 557, 570, 1000
HapII CCGG 1 cut(s) 553
Hin1II CATG 2 cut(s) 649, 941
HincII GTYRAC 3 cut(s) 28, 328, 828
HindII GTYRAC 3 cut(s) 28, 328, 828
HindIII AAGCTT 1 cut(s) 547
HinfI GANTC 5 cut(s) 265, 285, 383, 470, 659
HpaII CCGG 1 cut(s) 553
HphI GGTGA 2 cut(s) 936, 1032
Hpy166II GTNNAC 4 cut(s) 28, 132, 328, 828
Hpy188I TCNGA 4 cut(s) 388, 915, 958, 1084
Hpy188III TCNNGA 6 cut(s) 56, 361, 380, 398, 416, 467
Hpy8I GTNNAC 4 cut(s) 28, 132, 328, 828
HpyAV CCTTC 3 cut(s) 714, 891, 975
HpyCH4III ACNGT 3 cut(s) 136, 172, 616
HpyCH4IV ACGT 1 cut(s) 46
HpyCH4V TGCA 8 cut(s) 4, 212, 352, 440, 649, 745, 974, 1025
HpySE526I ACGT 1 cut(s) 46
Hsp92II CATG 2 cut(s) 649, 941
Kzo9I GATC 3 cut(s) 236, 609, 838
LguI GCTCTTC 1 cut(s) 116
LmnI GCTCC 5 cut(s) 85, 150, 817, 871, 1120
Lsp1109I GCAGC 5 cut(s) 154, 322, 452, 986, 1009
MaeI CTAG 4 cut(s) 222, 257, 719, 788
MaeII ACGT 1 cut(s) 46
MaeIII GTNAC 4 cut(s) 248, 651, 933, 1013
MalI GATC 3 cut(s) 238, 611, 840
MboI GATC 3 cut(s) 236, 609, 838
MboII GAAGA 5 cut(s) 133, 272, 292, 469, 617
MflI RGATCY 1 cut(s) 236
MluCI AATT 3 cut(s) 405, 506, 730
MlyI GAGTC 3 cut(s) 274, 294, 377
MmeI TCCRAC 1 cut(s) 667
MnlI CCTC 6 cut(s) 159, 368, 486, 506, 509, 1084
MspA1I CMGCKG 1 cut(s) 977
MspI CCGG 1 cut(s) 553
MspR9I CCNGG 4 cut(s) 554, 815, 948, 991
Mva1269I GAATGC 3 cut(s) 370, 442, 874
MvaI CCWGG 3 cut(s) 815, 948, 991
NciI CCSGG 1 cut(s) 554
NdeII GATC 3 cut(s) 236, 609, 838
NlaIII CATG 2 cut(s) 649, 941
NlaIV GGNNCC 2 cut(s) 19, 1116
NmeAIII GCCGAG 1 cut(s) 781
NmuCI GTSAC 1 cut(s) 933
NspI RCATGY 1 cut(s) 649
PciSI GCTCTTC 1 cut(s) 116
PctI GAATGC 3 cut(s) 370, 442, 874
PfeI GAWTC 2 cut(s) 470, 659
PflMI CCANNNNNTGG 1 cut(s) 996
PfoI TCCNGGA 1 cut(s) 989
PkrI GCNGC 6 cut(s) 144, 337, 442, 569, 976, 1024
PleI GAGTC 3 cut(s) 273, 293, 377
PpsI GAGTC 3 cut(s) 273, 293, 377
PsiI TTATAA 1 cut(s) 428
Psp6I CCWGG 3 cut(s) 813, 946, 989
PspFI CCCAGC 1 cut(s) 633
PspGI CCWGG 3 cut(s) 813, 946, 989
PspN4I GGNNCC 2 cut(s) 19, 1116
PspPI GGNCC 2 cut(s) 555, 1093
PstI CTGCAG 1 cut(s) 1027
PsuI RGATCY 1 cut(s) 236
RsaI GTAC 3 cut(s) 177, 321, 1033
RsaNI GTAC 3 cut(s) 176, 320, 1032
SapI GCTCTTC 1 cut(s) 116
SatI GCNGC 6 cut(s) 143, 336, 441, 568, 975, 1023
Sau3AI GATC 3 cut(s) 236, 609, 838
Sau96I GGNCC 2 cut(s) 555, 1093
ScaI AGTACT 1 cut(s) 177
SchI GAGTC 3 cut(s) 274, 294, 377
ScrFI CCNGG 4 cut(s) 554, 815, 948, 991
SfcI CTRYAG 2 cut(s) 529, 1023
SinI GGWCC 1 cut(s) 1093
SpeI ACTAGT 1 cut(s) 787
Sse9I AATT 3 cut(s) 405, 506, 730
SsiI CCGC 2 cut(s) 567, 977
SspMI CTAG 4 cut(s) 222, 257, 719, 788
StyD4I CCNGG 4 cut(s) 552, 813, 946, 989
StyI CCWWGG 3 cut(s) 756, 891, 1096
TaaI ACNGT 3 cut(s) 136, 172, 616
TaiI ACGT 1 cut(s) 49
TasI AATT 3 cut(s) 405, 506, 730
TatI WGTACW 1 cut(s) 175
TauI GCSGC 1 cut(s) 570
TfiI GAWTC 2 cut(s) 470, 659
TscAI CASTG 2 cut(s) 205, 619
TseFI GTSAC 1 cut(s) 933
TseI GCWGC 5 cut(s) 142, 335, 440, 974, 1022
Tsp45I GTSAC 1 cut(s) 933
TspDTI ATGAA 3 cut(s) 490, 530, 636
TspRI CASTG 2 cut(s) 205, 619
Van91I CCANNNNNTGG 1 cut(s) 996
VpaK11BI GGWCC 1 cut(s) 1093
XceI RCATGY 1 cut(s) 649
XspI CTAG 4 cut(s) 222, 257, 719, 788
ZrmI AGTACT 1 cut(s) 177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.