Rroxscaffold_5G00347500

Argonaute

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001623
Physical Location & Seq
Reverse (-)
18950143 .. 18952128
1986 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00347500.1

Sequence Viewer

Length: 699 bp
ATGGCCTCCAAGAGCATCTTGAAGGAGCTTAAGGATTTGCGAAGGATCCTCCCACTTCCTTCACACAGGTACACTCAAAGATTTATTATTTACAAACTAAATTTAGGTGTTCCTAGCATACCCCATAAATCTAATGCTGAGTTTTACCATTATAGTATTGCCATGCAATATGATGATGGAACCGAAGTTGACGGGAAAGGCATTAGACGTAAGGTTCTTGAAAAAGTAAAAGACACATATAGTGTGGGGCTGGAAAACAAGGATTTTGCTTATGATGGAGAGAAGAGCTTGTTTCTTGTTGGGCAGCTCCCTCATAAAAAACTTGATTTCACGGTAGTACCGGATGACATATCATCAAGCAGATGTGTCAACTACAATGATTGTAAAACCTGGTCCTGTTTGGAATTTCCTCATGGAGAACCGAATGTCAAAACTCCTTACCGATTGATTGGATTAAGGTTCTTTTTGAAATCAAAAGGAGGACACGATGGAGATGGAGAAGAGATCACTGTTTATGCATACTTTGCCCAGCATAAGCACATGCGATTACGCGATTCAGCAGATTTTCCCTGCATCAATGTTGGAAAACCAAAGAAACCTTCTTACTTTCCCCTAGAGCTTGGTGGAATCAACTCCTTGCTAATGGTGGAGCATTCTCCTTCTCTGCCCTTGGTATCCAAGTCCCCCACTCGAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

232

Amino Acids

26.42

Weight (kDa)

9.1

Isoelectric Point (pI)

39.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArgoN PF16486 44 - 116 1.2e-08 N-terminal domain of argonaute
PAZ PF02170 164 - 209 4e-08 PAZ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 552
AclWI GGATC 2 cut(s) 40, 53
AcsI RAATTY 2 cut(s) 100, 404
AfaI GTAC 2 cut(s) 71, 339
AflII CTTAAG 1 cut(s) 29
AgsI TTSAA 3 cut(s) 22, 221, 469
AjnI CCWGG 1 cut(s) 389
AjuI GAANNNNNNNTTGG 2 cut(s) 583, 615
AluBI AGCT 4 cut(s) 28, 288, 307, 619
AluI AGCT 4 cut(s) 28, 288, 307, 619
AlwI GGATC 2 cut(s) 40, 53
Ama87I CYCGRG 1 cut(s) 690
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 1 cut(s) 304
ApoI RAATTY 2 cut(s) 100, 404
AspS9I GGNCC 1 cut(s) 393
AvaI CYCGRG 1 cut(s) 690
AvaII GGWCC 1 cut(s) 393
BaeI ACNNNNGTAYC 2 cut(s) 321, 354
BamHI GGATCC 1 cut(s) 45
BbvI GCAGC 1 cut(s) 316
BccI CCATC 4 cut(s) 170, 269, 482, 488
BciT130I CCWGG 1 cut(s) 391
BciVI GTATCC 1 cut(s) 685
BfaI CTAG 2 cut(s) 114, 614
BfrI CTTAAG 1 cut(s) 29
BfuI GTATCC 1 cut(s) 685
BisI GCNGC 1 cut(s) 305
BlsI GCNGC 1 cut(s) 306
Bme1390I CCNGG 1 cut(s) 391
Bme18I GGWCC 1 cut(s) 393
BmeT110I CYCGRG 1 cut(s) 690
BmgT120I GGNCC 1 cut(s) 393
BmiI GGNNCC 2 cut(s) 47, 181
BmrFI CCNGG 1 cut(s) 391
BmsI GCATC 2 cut(s) 24, 582
BsaJI CCNNGG 1 cut(s) 669
BsaWI WCCGGW 1 cut(s) 340
BseBI CCWGG 1 cut(s) 391
BseDI CCNNGG 1 cut(s) 669
BseGI GGATG 1 cut(s) 349
BseMII CTCAG 1 cut(s) 129
BseXI GCAGC 1 cut(s) 316
BseYI CCCAGC 1 cut(s) 528
Bsh1236I CGCG 1 cut(s) 552
BshFI GGCC 1 cut(s) 5
BsiHKCI CYCGRG 1 cut(s) 690
BsiSI CCGG 1 cut(s) 341
BslFI GGGAC 1 cut(s) 667
BsmFI GGGAC 1 cut(s) 667
BsmI GAATGC 1 cut(s) 652
BsnI GGCC 1 cut(s) 5
BsoBI CYCGRG 1 cut(s) 690
Bsp143I GATC 2 cut(s) 45, 504
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 130
BspFNI CGCG 1 cut(s) 552
BspLI GGNNCC 2 cut(s) 47, 181
BspPI GGATC 2 cut(s) 40, 53
BspQI GCTCTTC 1 cut(s) 278
BspTI CTTAAG 1 cut(s) 29
BssECI CCNNGG 1 cut(s) 669
BssMI GATC 2 cut(s) 45, 504
BssT1I CCWWGG 1 cut(s) 669
Bst2UI CCWGG 1 cut(s) 391
Bst4CI ACNGT 2 cut(s) 334, 511
Bst6I CTCTTC 2 cut(s) 278, 495
BstAFI CTTAAG 1 cut(s) 29
BstAPI GCANNNNNTGC 1 cut(s) 524
BstDEI CTNAG 1 cut(s) 138
BstF5I GGATG 1 cut(s) 349
BstFNI CGCG 1 cut(s) 552
BstKTI GATC 2 cut(s) 48, 507
BstMBI GATC 2 cut(s) 45, 504
BstMWI GCNNNNNNNGC 1 cut(s) 524
BstNI CCWGG 1 cut(s) 391
BstNSI RCATGY 1 cut(s) 544
BstSCI CCNGG 1 cut(s) 389
BstUI CGCG 1 cut(s) 552
BstV1I GCAGC 1 cut(s) 316
BstX2I RGATCY 1 cut(s) 45
BstYI RGATCY 1 cut(s) 45
BsuI GTATCC 1 cut(s) 685
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 1 cut(s) 349
BtsIMutI CAGTG 1 cut(s) 507
Cfr13I GGNCC 1 cut(s) 393
CsiI ACCWGGT 1 cut(s) 389
Csp6I GTAC 2 cut(s) 70, 338
CviAII CATG 3 cut(s) 163, 413, 541
CviJI RGCY 6 cut(s) 5, 28, 250, 288, 307, 619
CviKI_1 RGCY 6 cut(s) 5, 28, 250, 288, 307, 619
CviQI GTAC 2 cut(s) 70, 338
DdeI CTNAG 1 cut(s) 138
DpnI GATC 2 cut(s) 47, 506
DpnII GATC 2 cut(s) 45, 504
Eam1104I CTCTTC 2 cut(s) 278, 495
EarI CTCTTC 2 cut(s) 278, 495
Eco130I CCWWGG 1 cut(s) 669
Eco47I GGWCC 1 cut(s) 393
Eco88I CYCGRG 1 cut(s) 690
EcoRII CCWGG 1 cut(s) 389
EcoT14I CCWWGG 1 cut(s) 669
EcoT22I ATGCAT 1 cut(s) 520
ErhI CCWWGG 1 cut(s) 669
FaeI CATG 3 cut(s) 166, 416, 544
FalI AAGNNNNNCTT 1 cut(s) 34
FaqI GGGAC 1 cut(s) 667
FatI CATG 3 cut(s) 162, 412, 540
Fnu4HI GCNGC 1 cut(s) 305
FokI GGATG 1 cut(s) 356
Fsp4HI GCNGC 1 cut(s) 305
FspBI CTAG 2 cut(s) 114, 614
GluI GCNGC 1 cut(s) 305
GsaI CCCAGC 1 cut(s) 532
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 341
Hin1II CATG 3 cut(s) 166, 416, 544
HincII GTYRAC 2 cut(s) 190, 370
HindII GTYRAC 2 cut(s) 190, 370
HinfI GANTC 2 cut(s) 554, 627
HpaII CCGG 1 cut(s) 341
Hpy166II GTNNAC 3 cut(s) 72, 190, 370
Hpy188III TCNNGA 2 cut(s) 19, 218
Hpy8I GTNNAC 3 cut(s) 72, 190, 370
HpyAV CCTTC 5 cut(s) 16, 36, 69, 609, 669
HpyCH4III ACNGT 2 cut(s) 334, 511
HpyCH4IV ACGT 1 cut(s) 208
HpyCH4V TGCA 3 cut(s) 166, 518, 573
HpyF10VI GCNNNNNNNGC 1 cut(s) 524
HpyF3I CTNAG 1 cut(s) 138
HpySE526I ACGT 1 cut(s) 208
Hsp92II CATG 3 cut(s) 166, 416, 544
Kzo9I GATC 2 cut(s) 45, 504
LguI GCTCTTC 1 cut(s) 278
LmnI GCTCC 3 cut(s) 25, 312, 649
LpnPI CCDG 8 cut(s) 52, 236, 354, 376, 403, 409, 542, 583
Lsp1109I GCAGC 1 cut(s) 316
LweI GCATC 2 cut(s) 24, 582
MabI ACCWGGT 1 cut(s) 389
MaeI CTAG 2 cut(s) 114, 614
MaeII ACGT 1 cut(s) 208
MalI GATC 2 cut(s) 47, 506
MboI GATC 2 cut(s) 45, 504
MboII GAAGA 2 cut(s) 295, 512
MflI RGATCY 1 cut(s) 45
MluCI AATT 2 cut(s) 100, 404
MmeI TCCRAC 1 cut(s) 562
MnlI CCTC 5 cut(s) 16, 59, 321, 420, 473
Mph1103I ATGCAT 1 cut(s) 520
MseI TTAA 2 cut(s) 30, 455
MspCI CTTAAG 1 cut(s) 29
MspI CCGG 1 cut(s) 341
MspR9I CCNGG 1 cut(s) 391
Mva1269I GAATGC 1 cut(s) 652
MvaI CCWGG 1 cut(s) 391
MvnI CGCG 1 cut(s) 552
MwoI GCNNNNNNNGC 1 cut(s) 524
NdeII GATC 2 cut(s) 45, 504
NlaIII CATG 3 cut(s) 166, 416, 544
NlaIV GGNNCC 2 cut(s) 47, 181
NsiI ATGCAT 1 cut(s) 520
NspI RCATGY 1 cut(s) 544
PaeR7I CTCGAG 1 cut(s) 690
PciSI GCTCTTC 1 cut(s) 278
PctI GAATGC 1 cut(s) 652
PfeI GAWTC 2 cut(s) 554, 627
PkrI GCNGC 1 cut(s) 306
Psp6I CCWGG 1 cut(s) 389
PspFI CCCAGC 1 cut(s) 528
PspGI CCWGG 1 cut(s) 389
PspN4I GGNNCC 2 cut(s) 47, 181
PspPI GGNCC 1 cut(s) 393
PspXI VCTCGAGB 1 cut(s) 690
PsuI RGATCY 1 cut(s) 45
RsaI GTAC 2 cut(s) 71, 339
RsaNI GTAC 2 cut(s) 70, 338
SapI GCTCTTC 1 cut(s) 278
SaqAI TTAA 2 cut(s) 30, 455
SatI GCNGC 1 cut(s) 305
Sau3AI GATC 2 cut(s) 45, 504
Sau96I GGNCC 1 cut(s) 393
ScrFI CCNGG 1 cut(s) 391
SexAI ACCWGGT 1 cut(s) 389
SfaNI GCATC 2 cut(s) 24, 582
Sfr274I CTCGAG 1 cut(s) 690
SinI GGWCC 1 cut(s) 393
SlaI CTCGAG 1 cut(s) 690
SmlI CTYRAG 2 cut(s) 29, 690
SmoI CTYRAG 2 cut(s) 29, 690
Sse9I AATT 2 cut(s) 100, 404
SspMI CTAG 2 cut(s) 114, 614
StyD4I CCNGG 1 cut(s) 389
StyI CCWWGG 1 cut(s) 669
TaaI ACNGT 2 cut(s) 334, 511
TaiI ACGT 1 cut(s) 211
TaqI TCGA 1 cut(s) 691
TasI AATT 2 cut(s) 100, 404
TfiI GAWTC 2 cut(s) 554, 627
Tru1I TTAA 2 cut(s) 30, 455
Tru9I TTAA 2 cut(s) 30, 455
TscAI CASTG 1 cut(s) 514
TseI GCWGC 1 cut(s) 304
TspRI CASTG 1 cut(s) 514
Vha464I CTTAAG 1 cut(s) 29
VpaK11BI GGWCC 1 cut(s) 393
XapI RAATTY 2 cut(s) 100, 404
XceI RCATGY 1 cut(s) 544
XhoI CTCGAG 1 cut(s) 690
XspI CTAG 2 cut(s) 114, 614
Zsp2I ATGCAT 1 cut(s) 520
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.