Rw1G008560

Argonaute

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0001623
Physical Location & Seq
Reverse (-)
18542475 .. 18543874
1400 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G008560.1

Sequence Viewer

Length: 528 bp
ATGTTGGATGGAGTGAGTGAATCACAGTTCAACCAAGTCTTGAATGTAGAACTGGATCAGATTATTGAGGCCTGCAAGTTTCTTGACGAGAGCTGGTGTCCAAAGTCCATGGTTATTTTTGCACAAAAGAATCATCATACAAAATTTTTTCAGGCTGGTGGTTCTCCTGACAATGTTCCACCTGGGACTATTATTGACAACAAAGTCTGTCATCCAAAGAACAATGATTTCTACTTATGTTCTCATGCTGGGATTATTGGAACAACTCGTCCTACTCATTATCATGTTCTATATGATGAACATGGCTTCTCAGCGGATGACTTCACAATGGCCATATCTGTAGTTGCTCCGGTATGCTATGCCCACCTAGCAGCAGCACAGATGAGTCAGTTTATTAAGTTCGATGAAATATCAGAGACTGCTTCTAGCCAAGGTGGTTCTGGTGGTGTTACTGTTGCTGGAGCTGTGCCAGTGCAGGAGGTACCAAGGCTACATAAAAGTGTCGTCAATTACATGTTCTTCTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

175

Amino Acids

19.19

Weight (kDa)

5.46

Isoelectric Point (pI)

49.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Piwi PF02171 3 - 133 1e-43 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 203
Acc65I GGTACC 1 cut(s) 481
AccB1I GGYRCC 1 cut(s) 481
AciI CCGC 1 cut(s) 314
AclWI GGATC 1 cut(s) 63
AcoI YGGCCR 1 cut(s) 330
AcsI RAATTY 1 cut(s) 143
AfaI GTAC 1 cut(s) 483
AflIII ACRYGT 1 cut(s) 513
AgsI TTSAA 2 cut(s) 31, 43
AjnI CCWGG 1 cut(s) 181
AloI GAACNNNNNNTCC 2 cut(s) 253, 285
AluBI AGCT 2 cut(s) 93, 464
AluI AGCT 2 cut(s) 93, 464
Alw26I GTCTC 1 cut(s) 410
AlwI GGATC 1 cut(s) 63
AlwNI CAGNNNCTG 1 cut(s) 419
AoxI GGCC 2 cut(s) 69, 330
ApeKI GCWGC 2 cut(s) 371, 374
ApoI RAATTY 1 cut(s) 143
Asp718I GGTACC 1 cut(s) 481
BalI TGGCCA 1 cut(s) 332
BanI GGYRCC 1 cut(s) 481
BbvI GCAGC 2 cut(s) 383, 386
BccI CCATC 1 cut(s) 2
BciT130I CCWGG 1 cut(s) 183
BcoDI GTCTC 1 cut(s) 410
BfaI CTAG 2 cut(s) 368, 426
BfmI CTRYAG 1 cut(s) 339
BisI GCNGC 2 cut(s) 372, 375
BlsI GCNGC 2 cut(s) 373, 376
Bme1390I CCNGG 1 cut(s) 183
BmiI GGNNCC 1 cut(s) 483
BmrFI CCNGG 1 cut(s) 183
BpmI CTGGAG 1 cut(s) 480
BsaJI CCNNGG 4 cut(s) 108, 182, 430, 485
BsaWI WCCGGW 1 cut(s) 349
Bse1I ACTGG 2 cut(s) 57, 470
BseBI CCWGG 1 cut(s) 183
BseDI CCNNGG 4 cut(s) 108, 182, 430, 485
BseGI GGATG 3 cut(s) 13, 211, 322
BseMII CTCAG 1 cut(s) 324
BseNI ACTGG 2 cut(s) 57, 470
BseXI GCAGC 2 cut(s) 383, 386
BseYI CCCAGC 1 cut(s) 248
BsgI GTGCAG 1 cut(s) 494
BshFI GGCC 2 cut(s) 71, 332
BshNI GGYRCC 1 cut(s) 481
BsiSI CCGG 1 cut(s) 350
BslFI GGGAC 1 cut(s) 199
BsmAI GTCTC 1 cut(s) 410
BsmFI GGGAC 1 cut(s) 199
BsnI GGCC 2 cut(s) 71, 332
Bsp143I GATC 1 cut(s) 55
Bsp19I CCATGG 1 cut(s) 108
BspACI CCGC 1 cut(s) 314
BspANI GGCC 2 cut(s) 71, 332
BspCNI CTCAG 1 cut(s) 323
BspLI GGNNCC 1 cut(s) 483
BspPI GGATC 1 cut(s) 63
BspT107I GGYRCC 1 cut(s) 481
BsrI ACTGG 2 cut(s) 57, 470
BssECI CCNNGG 4 cut(s) 108, 182, 430, 485
BssMI GATC 1 cut(s) 55
BssT1I CCWWGG 3 cut(s) 108, 430, 485
Bst2UI CCWGG 1 cut(s) 183
Bst4CI ACNGT 2 cut(s) 27, 454
BstC8I GCNNGC 1 cut(s) 73
BstDEI CTNAG 1 cut(s) 310
BstDSI CCRYGG 1 cut(s) 108
BstF5I GGATG 3 cut(s) 13, 211, 322
BstKTI GATC 1 cut(s) 58
BstMAI GTCTC 1 cut(s) 410
BstMBI GATC 1 cut(s) 55
BstMWI GCNNNNNNNGC 1 cut(s) 368
BstNI CCWGG 1 cut(s) 183
BstNSI RCATGY 1 cut(s) 517
BstSCI CCNGG 1 cut(s) 181
BstSFI CTRYAG 1 cut(s) 339
BstV1I GCAGC 2 cut(s) 383, 386
BsuRI GGCC 2 cut(s) 71, 332
BtgI CCRYGG 1 cut(s) 108
BtsCI GGATG 3 cut(s) 13, 211, 322
BtsIMutI CAGTG 1 cut(s) 477
Cac8I GCNNGC 1 cut(s) 73
CaiI CAGNNNCTG 1 cut(s) 419
Csp6I GTAC 1 cut(s) 482
CviAII CATG 5 cut(s) 109, 245, 284, 302, 514
CviJI RGCY 8 cut(s) 71, 93, 155, 306, 332, 429, 464, 490
CviKI_1 RGCY 8 cut(s) 71, 93, 155, 306, 332, 429, 464, 490
CviQI GTAC 1 cut(s) 482
DdeI CTNAG 1 cut(s) 310
DpnI GATC 1 cut(s) 57
DpnII GATC 1 cut(s) 55
DrdI GACNNNNNNGTC 1 cut(s) 203
DseDI GACNNNNNNGTC 1 cut(s) 203
EaeI YGGCCR 1 cut(s) 330
Eco130I CCWWGG 3 cut(s) 108, 430, 485
Eco147I AGGCCT 1 cut(s) 71
EcoRII CCWGG 1 cut(s) 181
EcoT14I CCWWGG 3 cut(s) 108, 430, 485
ErhI CCWWGG 3 cut(s) 108, 430, 485
FaeI CATG 5 cut(s) 112, 248, 287, 305, 517
FaqI GGGAC 1 cut(s) 199
FatI CATG 5 cut(s) 108, 244, 283, 301, 513
Fnu4HI GCNGC 2 cut(s) 372, 375
FokI GGATG 3 cut(s) 20, 198, 329
Fsp4HI GCNGC 2 cut(s) 372, 375
FspBI CTAG 2 cut(s) 368, 426
GluI GCNGC 2 cut(s) 372, 375
GsaI CCCAGC 1 cut(s) 252
GsuI CTGGAG 1 cut(s) 480
HaeIII GGCC 2 cut(s) 71, 332
HapII CCGG 1 cut(s) 350
Hin1II CATG 5 cut(s) 112, 248, 287, 305, 517
HinfI GANTC 3 cut(s) 20, 130, 385
HpaII CCGG 1 cut(s) 350
Hpy188I TCNGA 2 cut(s) 60, 415
Hpy188III TCNNGA 3 cut(s) 40, 83, 167
HpyCH4III ACNGT 2 cut(s) 27, 454
HpyCH4V TGCA 3 cut(s) 75, 122, 475
HpyF10VI GCNNNNNNNGC 1 cut(s) 368
HpyF3I CTNAG 1 cut(s) 310
Hsp92II CATG 5 cut(s) 112, 248, 287, 305, 517
KpnI GGTACC 1 cut(s) 485
Kzo9I GATC 1 cut(s) 55
LmnI GCTCC 2 cut(s) 352, 461
Lsp1109I GCAGC 2 cut(s) 383, 386
MaeI CTAG 2 cut(s) 368, 426
MaeIII GTNAC 1 cut(s) 448
MalI GATC 1 cut(s) 57
MboI GATC 1 cut(s) 55
MboII GAAGA 1 cut(s) 511
MlsI TGGCCA 1 cut(s) 332
MluCI AATT 2 cut(s) 143, 508
MluNI TGGCCA 1 cut(s) 332
MlyI GAGTC 1 cut(s) 394
MnlI CCTC 2 cut(s) 61, 472
Mox20I TGGCCA 1 cut(s) 332
MscI TGGCCA 1 cut(s) 332
MseI TTAA 1 cut(s) 396
MslI CAYNNNNRTG 2 cut(s) 282, 498
Msp20I TGGCCA 1 cut(s) 332
MspA1I CMGCKG 1 cut(s) 314
MspI CCGG 1 cut(s) 350
MspR9I CCNGG 1 cut(s) 183
MvaI CCWGG 1 cut(s) 183
MwoI GCNNNNNNNGC 1 cut(s) 368
NcoI CCATGG 1 cut(s) 108
NdeII GATC 1 cut(s) 55
NlaIII CATG 5 cut(s) 112, 248, 287, 305, 517
NlaIV GGNNCC 1 cut(s) 483
NspI RCATGY 1 cut(s) 517
PceI AGGCCT 1 cut(s) 71
PciI ACATGT 1 cut(s) 513
PfeI GAWTC 2 cut(s) 20, 130
PkrI GCNGC 2 cut(s) 373, 376
PleI GAGTC 1 cut(s) 393
PpsI GAGTC 1 cut(s) 393
PscI ACATGT 1 cut(s) 513
Psp6I CCWGG 1 cut(s) 181
PspFI CCCAGC 1 cut(s) 248
PspGI CCWGG 1 cut(s) 181
PspN4I GGNNCC 1 cut(s) 483
PstNI CAGNNNCTG 1 cut(s) 419
RsaI GTAC 1 cut(s) 483
RsaNI GTAC 1 cut(s) 482
RseI CAYNNNNRTG 2 cut(s) 282, 498
SaqAI TTAA 1 cut(s) 396
SatI GCNGC 2 cut(s) 372, 375
Sau3AI GATC 1 cut(s) 55
SchI GAGTC 1 cut(s) 394
ScrFI CCNGG 1 cut(s) 183
SetI ASST 6 cut(s) 95, 184, 369, 436, 466, 483
SfcI CTRYAG 1 cut(s) 339
SmiMI CAYNNNNRTG 2 cut(s) 282, 498
Sse9I AATT 2 cut(s) 143, 508
SseBI AGGCCT 1 cut(s) 71
SsiI CCGC 1 cut(s) 314
SspMI CTAG 2 cut(s) 368, 426
StuI AGGCCT 1 cut(s) 71
StyD4I CCNGG 1 cut(s) 181
StyI CCWWGG 3 cut(s) 108, 430, 485
TaaI ACNGT 2 cut(s) 27, 454
TaqI TCGA 1 cut(s) 402
TasI AATT 2 cut(s) 143, 508
TfiI GAWTC 2 cut(s) 20, 130
Tru1I TTAA 1 cut(s) 396
Tru9I TTAA 1 cut(s) 396
TscAI CASTG 1 cut(s) 477
TseI GCWGC 2 cut(s) 371, 374
TspDTI ATGAA 2 cut(s) 312, 420
TspRI CASTG 1 cut(s) 477
XapI RAATTY 1 cut(s) 143
XceI RCATGY 1 cut(s) 517
XcmI CCANNNNNNNNNTGG 1 cut(s) 437
XspI CTAG 2 cut(s) 368, 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.