Rh3CG350500

Argonaute

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0001623
Physical Location & Seq
Forward (+)
42059110 .. 42095965
36856 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG350500.1

Sequence Viewer

Length: 594 bp
ATGATGTTACAGATTGCCATGCAACATGATGATGGAACCGAAGTTGACGGGAAAGGCATTGGACGTAAGGTTCTTGAAAAAGTAAAAGACACATATAGTGTGGAGCTGGAAAACAAGGATTTTGCTTATGATGGAGAGAAGAGCTTGTTTACTGTTGGGCAGCTCCCTCATAAAAAACTTGATTTCACAATAGTACTGGATGACATATCATCAAGCAGGTCTAGGACGAATGGGGGATCTGGGGGCAGTTACTCCCTAGAAGAGAGTCAGGGCGATAGGAAGAGAGTCAAGCAGTCATTTCAATCCAAAACAATAAAGGTACAGGTCAACTTTGCTGCTAAAATCCCAATGAAAGCAATCACGAATGCCTTGCGAGGTCTGGACTCTGACCATTTTCAGGAAGCAGTTACAGTTCTTGACATCATTATAAGGCAGAATGCAGCAAAGCAGCGTTCGTGTGGTCCTATTGCCGCTGTTCCTTTGCACATTGTTTGGTATTATTTCAACGTTGTATACTTCTTTAGCTCTTTGGGACAGTGTGCTATCAAGCCCTTCGGCTATTTCAAATTCAGGTCCTACTATCTTCATACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

197

Amino Acids

22.19

Weight (kDa)

9.1

Isoelectric Point (pI)

35.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArgoN PF16486 14 - 143 5.5e-18 N-terminal domain of argonaute
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 428
Acc36I ACCTGC 1 cut(s) 207
AccI GTMKAC 1 cut(s) 513
AciI CCGC 1 cut(s) 471
AclI AACGTT 1 cut(s) 507
AclWI GGATC 1 cut(s) 244
AcsI RAATTY 1 cut(s) 566
AfaI GTAC 2 cut(s) 195, 321
AfiI CCNNNNNNNGG 1 cut(s) 397
AgsI TTSAA 4 cut(s) 77, 302, 505, 565
AloI GAACNNNNNNTCC 2 cut(s) 54, 86
AluBI AGCT 4 cut(s) 106, 144, 163, 525
AluI AGCT 4 cut(s) 106, 144, 163, 525
AlwI GGATC 1 cut(s) 244
ApeKI GCWGC 4 cut(s) 160, 335, 440, 448
ApoI RAATTY 1 cut(s) 566
AspS9I GGNCC 2 cut(s) 461, 573
AvaII GGWCC 2 cut(s) 461, 573
BbvI GCAGC 4 cut(s) 172, 322, 452, 460
BccI CCATC 2 cut(s) 26, 125
BcgI CGANNNNNNTGC 2 cut(s) 352, 386
BfaI CTAG 2 cut(s) 222, 257
BfuAI ACCTGC 1 cut(s) 207
BisI GCNGC 5 cut(s) 161, 336, 441, 449, 471
BlsI GCNGC 5 cut(s) 162, 337, 442, 450, 472
BmcAI AGTACT 1 cut(s) 195
Bme18I GGWCC 2 cut(s) 461, 573
BmgT120I GGNCC 2 cut(s) 461, 573
BmiI GGNNCC 1 cut(s) 37
Bsc4I CCNNNNNNNGG 1 cut(s) 397
Bse1I ACTGG 1 cut(s) 201
BseGI GGATG 1 cut(s) 205
BseLI CCNNNNNNNGG 1 cut(s) 397
BseNI ACTGG 1 cut(s) 201
BseXI GCAGC 4 cut(s) 172, 322, 452, 460
BslFI GGGAC 1 cut(s) 546
BslI CCNNNNNNNGG 1 cut(s) 397
BsmFI GGGAC 1 cut(s) 546
BsmI GAATGC 2 cut(s) 370, 442
Bsp143I GATC 1 cut(s) 236
BspACI CCGC 1 cut(s) 471
BspLI GGNNCC 1 cut(s) 37
BspMI ACCTGC 1 cut(s) 207
BspPI GGATC 1 cut(s) 244
BspQI GCTCTTC 1 cut(s) 134
BsrI ACTGG 1 cut(s) 201
BssMI GATC 1 cut(s) 236
BssNAI GTATAC 1 cut(s) 514
Bst1107I GTATAC 1 cut(s) 514
Bst4CI ACNGT 3 cut(s) 154, 412, 537
Bst6I CTCTTC 3 cut(s) 134, 255, 275
BstF5I GGATG 1 cut(s) 205
BstKTI GATC 1 cut(s) 239
BstMBI GATC 1 cut(s) 236
BstV1I GCAGC 4 cut(s) 172, 322, 452, 460
BstX2I RGATCY 1 cut(s) 236
BstYI RGATCY 1 cut(s) 236
BstZ17I GTATAC 1 cut(s) 514
BtsCI GGATG 1 cut(s) 205
BtsIMutI CAGTG 1 cut(s) 542
BveI ACCTGC 1 cut(s) 207
Cfr13I GGNCC 2 cut(s) 461, 573
Csp6I GTAC 2 cut(s) 194, 320
CviAII CATG 2 cut(s) 19, 26
CviJI RGCY 6 cut(s) 106, 144, 163, 525, 550, 558
CviKI_1 RGCY 6 cut(s) 106, 144, 163, 525, 550, 558
CviQI GTAC 2 cut(s) 194, 320
DpnI GATC 1 cut(s) 238
DpnII GATC 1 cut(s) 236
Eam1104I CTCTTC 3 cut(s) 134, 255, 275
EarI CTCTTC 3 cut(s) 134, 255, 275
Eco47I GGWCC 2 cut(s) 461, 573
EcoO109I RGGNCCY 1 cut(s) 573
FaeI CATG 2 cut(s) 22, 29
FaqI GGGAC 1 cut(s) 546
FatI CATG 2 cut(s) 18, 25
FblI GTMKAC 1 cut(s) 513
Fnu4HI GCNGC 5 cut(s) 161, 336, 441, 449, 471
FokI GGATG 1 cut(s) 212
Fsp4HI GCNGC 5 cut(s) 161, 336, 441, 449, 471
FspBI CTAG 2 cut(s) 222, 257
GluI GCNGC 5 cut(s) 161, 336, 441, 449, 471
Hin1II CATG 2 cut(s) 22, 29
HincII GTYRAC 2 cut(s) 46, 328
HindII GTYRAC 2 cut(s) 46, 328
HinfI GANTC 3 cut(s) 265, 285, 383
Hpy166II GTNNAC 4 cut(s) 46, 150, 328, 514
Hpy188I TCNGA 1 cut(s) 388
Hpy188III TCNNGA 5 cut(s) 74, 361, 380, 398, 416
Hpy8I GTNNAC 4 cut(s) 46, 150, 328, 514
HpyAV CCTTC 1 cut(s) 562
HpyCH4III ACNGT 3 cut(s) 154, 412, 537
HpyCH4IV ACGT 2 cut(s) 64, 507
HpyCH4V TGCA 3 cut(s) 22, 440, 484
HpySE526I ACGT 2 cut(s) 64, 507
Hsp92II CATG 2 cut(s) 22, 29
Kzo9I GATC 1 cut(s) 236
LguI GCTCTTC 1 cut(s) 134
LmnI GCTCC 2 cut(s) 103, 168
LpnPI CCDG 9 cut(s) 92, 182, 202, 225, 254, 308, 365, 383, 556
Lsp1109I GCAGC 4 cut(s) 172, 322, 452, 460
MaeI CTAG 2 cut(s) 222, 257
MaeII ACGT 2 cut(s) 64, 507
MaeIII GTNAC 3 cut(s) 6, 248, 406
MalI GATC 1 cut(s) 238
MboI GATC 1 cut(s) 236
MboII GAAGA 4 cut(s) 151, 272, 292, 575
MflI RGATCY 1 cut(s) 236
MluCI AATT 1 cut(s) 566
MlyI GAGTC 3 cut(s) 274, 294, 377
MnlI CCTC 2 cut(s) 177, 368
MslI CAYNNNNRTG 1 cut(s) 30
MspA1I CMGCKG 1 cut(s) 473
Mva1269I GAATGC 2 cut(s) 370, 442
NdeII GATC 1 cut(s) 236
NlaIII CATG 2 cut(s) 22, 29
NlaIV GGNNCC 1 cut(s) 37
PciSI GCTCTTC 1 cut(s) 134
PctI GAATGC 2 cut(s) 370, 442
PkrI GCNGC 5 cut(s) 162, 337, 442, 450, 472
PleI GAGTC 3 cut(s) 273, 293, 377
PpsI GAGTC 3 cut(s) 273, 293, 377
PpuMI RGGWCCY 1 cut(s) 573
PsiI TTATAA 1 cut(s) 428
Psp1406I AACGTT 1 cut(s) 507
Psp5II RGGWCCY 1 cut(s) 573
PspN4I GGNNCC 1 cut(s) 37
PspPI GGNCC 2 cut(s) 461, 573
PspPPI RGGWCCY 1 cut(s) 573
PsuI RGATCY 1 cut(s) 236
RsaI GTAC 2 cut(s) 195, 321
RsaNI GTAC 2 cut(s) 194, 320
RseI CAYNNNNRTG 1 cut(s) 30
SapI GCTCTTC 1 cut(s) 134
SatI GCNGC 5 cut(s) 161, 336, 441, 449, 471
Sau3AI GATC 1 cut(s) 236
Sau96I GGNCC 2 cut(s) 461, 573
ScaI AGTACT 1 cut(s) 195
SchI GAGTC 3 cut(s) 274, 294, 377
SinI GGWCC 2 cut(s) 461, 573
SmiMI CAYNNNNRTG 1 cut(s) 30
Sse9I AATT 1 cut(s) 566
SsiI CCGC 1 cut(s) 471
SspMI CTAG 2 cut(s) 222, 257
TaaI ACNGT 3 cut(s) 154, 412, 537
TaiI ACGT 2 cut(s) 67, 510
TasI AATT 1 cut(s) 566
TatI WGTACW 1 cut(s) 193
TauI GCSGC 1 cut(s) 473
TscAI CASTG 1 cut(s) 542
TseI GCWGC 4 cut(s) 160, 335, 440, 448
TspDTI ATGAA 2 cut(s) 365, 575
TspRI CASTG 1 cut(s) 542
VpaK11BI GGWCC 2 cut(s) 461, 573
XapI RAATTY 1 cut(s) 566
XmiI GTMKAC 1 cut(s) 513
XspI CTAG 2 cut(s) 222, 257
ZrmI AGTACT 1 cut(s) 195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.